Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Probable malate dehydrogenase 2, mitochondrial

Gene

mdhB

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the reversible oxidation of malate to oxaloacetate.By similarity

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei112SubstrateBy similarity1
Binding sitei118SubstrateBy similarity1
Binding sitei125NADBy similarity1
Binding sitei132NADBy similarity1
Binding sitei152SubstrateBy similarity1
Binding sitei183SubstrateBy similarity1
Active sitei208Proton acceptorBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi31 – 37NADBy similarity7
Nucleotide bindingi150 – 152NADBy similarity3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processTricarboxylic acid cycle
LigandNAD

Names & Taxonomyi

Protein namesi
Recommended name:
Probable malate dehydrogenase 2, mitochondrial (EC:1.1.1.37)
Gene namesi
Name:mdhB
ORF Names:DDB_G0292600
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyostelidsDictyostelialesDictyosteliaceaeDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 6, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0292600 mdhB

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 9MitochondrionSequence analysis9
ChainiPRO_000031234810 – 348Probable malate dehydrogenase 2, mitochondrialAdd BLAST339

Proteomic databases

PaxDbiQ54D04
PRIDEiQ54D04

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi44689.DDB0230188

Structurei

3D structure databases

ProteinModelPortaliQ54D04
SMRiQ54D04
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 2 family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1496 Eukaryota
COG0039 LUCA
InParanoidiQ54D04
KOiK00024
OMAiAGQISNH
PhylomeDBiQ54D04

Family and domain databases

Gene3Di3.90.110.10, 1 hit
HAMAPiMF_01517 Malate_dehydrog_2, 1 hit
InterProiView protein in InterPro
IPR001557 L-lactate/malate_DH
IPR022383 Lactate/malate_DH_C
IPR001236 Lactate/malate_DH_N
IPR015955 Lactate_DH/Glyco_Ohase_4_C
IPR010945 Malate_DH_type2
IPR036291 NAD(P)-bd_dom_sf
PANTHERiPTHR23382 PTHR23382, 1 hit
PfamiView protein in Pfam
PF02866 Ldh_1_C, 1 hit
PF00056 Ldh_1_N, 1 hit
PIRSFiPIRSF000102 Lac_mal_DH, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
SSF56327 SSF56327, 1 hit
TIGRFAMsiTIGR01759 MalateDH-SF1, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q54D04-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKILTRSFS TGANFFASGK GKAPVRVAIT GASGQIGYQL LFRIASGDML
60 70 80 90 100
GKDQPIILQC LELPGAMNSL KGVSMELDDC AFPLLKGIVQ SDKPEEAFAG
110 120 130 140 150
ADYALLVGAR PRSKGMERGD LLKANAEIFS VQGKALDKSA NRDTLRVLVV
160 170 180 190 200
GNPANTNALI AARNAPNIDP KRFSAMTRLD HNRGLAQLAD KTGSAVTDIE
210 220 230 240 250
KFCIWGNHSA TQYPDINFGT VKGKSLVDTI NDQKWVKDNF IPTVQQRGAA
260 270 280 290 300
IIAARGLSSA ASAASAAIDH MRDWTYGTNG QWTSMAIYSE GEYGADKGLY
310 320 330 340
FSFPVIVDNK GKYEIVKGLK LDQFSQERFD ATRKELLSEM DGVKELLP
Length:348
Mass (Da):37,648
Last modified:May 24, 2005 - v1
Checksum:iA70092840B76C58B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000194 Genomic DNA Translation: EAL61103.1
RefSeqiXP_629516.1, XM_629514.1

Genome annotation databases

EnsemblProtistsiEAL61103; EAL61103; DDB_G0292600
GeneIDi8628767
KEGGiddi:DDB_G0292600

Similar proteinsi

Entry informationi

Entry nameiMDHB_DICDI
AccessioniPrimary (citable) accession number: Q54D04
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 4, 2007
Last sequence update: May 24, 2005
Last modified: May 23, 2018
This is version 88 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health