UniProtKB - Q545K4 (Q545K4_MOUSE)
Eukaryotic translation initiation factor 6
Eif6
Functioni
Binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit to form the 80S initiation complex in the cytoplasm. Behaves as a stimulatory translation initiation factor downstream insulin/growth factors. Is also involved in ribosome biogenesis. Associates with pre-60S subunits in the nucleus and is involved in its nuclear export. Cytoplasmic release of TIF6 from 60S subunits and nuclear relocalization is promoted by a RACK1 (RACK1)-dependent protein kinase C activity. In tissues responsive to insulin, controls fatty acid synthesis and glycolysis by exerting translational control of adipogenic transcription factors such as CEBPB, CEBPD and ATF4 that have G/C rich or uORF in their 5'UTR. Required for ROS-dependent megakaryocyte maturation and platelets formation, controls the expression of mitochondrial respiratory chain genes involved in reactive oxygen species (ROS) synthesis. Involved in miRNA-mediated gene silencing by the RNA-induced silencing complex (RISC). Required for both miRNA-mediated translational repression and miRNA-mediated cleavage of complementary mRNAs by RISC. Modulates cell cycle progression and global translation of pre-B cells, its activation seems to be rate-limiting in tumorigenesis and tumor growth.
UniRule annotationGO - Molecular functioni
- ribosomal large subunit binding Source: UniProtKB-UniRule
- ribosome binding Source: Ensembl
- translation initiation factor activity Source: UniProtKB-UniRule
GO - Biological processi
- mature ribosome assembly Source: UniProtKB-UniRule
- miRNA-mediated gene silencing by inhibition of translation Source: Ensembl
- ribosomal large subunit biogenesis Source: UniProtKB-UniRule
- ribosomal subunit export from nucleus Source: UniProtKB-UniRule
Keywordsi
Molecular function | Initiation factorUniRule annotationARBA annotation |
Biological process | Protein biosynthesis, Ribosome biogenesisUniRule annotation |
Names & Taxonomyi
Protein namesi | Recommended name: Eukaryotic translation initiation factor 6UniRule annotationShort name: eIF-6UniRule annotation |
Gene namesi | |
Organismi | Mus musculus (Mouse)Imported |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Organism-specific databases
MGIi | MGI:1196288, Eif6 |
VEuPathDBi | HostDB:ENSMUSG00000027613 |
Subcellular locationi
Nucleus
- nucleolus Source: UniProtKB-SubCell
- nucleoplasm Source: Ensembl
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmUniRule annotation, NucleusUniRule annotationPTM / Processingi
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 165 | PhosphothreonineUniRule annotation | 1 | |
Modified residuei | 166 | PhosphoserineUniRule annotation | 1 | |
Modified residuei | 174 | Phosphoserine; by CK1UniRule annotation | 1 | |
Modified residuei | 175 | Phosphoserine; by CK1UniRule annotation | 1 |
Post-translational modificationi
Keywords - PTMi
PhosphoproteinUniRule annotationExpressioni
Gene expression databases
Bgeei | ENSMUSG00000027613, Expressed in adult mammalian kidney and 319 other tissues |
Interactioni
Subunit structurei
Monomer. Associates with the 60S ribosomal subunit.
Interacts with RACK1.
Interacts with DICER1, AGO2, TARBP2, MOV10 and RPL7A; they form a large RNA-induced silencing complex (RISC).
UniRule annotationStructurei
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
HOGENOMi | CLU_071894_0_0_1 |
OMAi | GEDTTGP |
OrthoDBi | 1157302at2759 |
Family and domain databases
CDDi | cd00527, IF6, 1 hit |
HAMAPi | MF_00032, eIF_6, 1 hit |
InterProi | View protein in InterPro IPR002769, eIF6 |
PANTHERi | PTHR10784, PTHR10784, 1 hit |
Pfami | View protein in Pfam PF01912, eIF-6, 1 hit |
PIRSFi | PIRSF006413, IF-6, 1 hit |
SMARTi | View protein in SMART SM00654, eIF6, 1 hit |
TIGRFAMsi | TIGR00323, eIF-6, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MAVRASFENN CEVGCFAKLT NAYCLVAIGG SENFYSVFEG ELSDAIPVVH
60 70 80 90 100
ASIAGCRIIG RMCVGNRHGL LVPNNTTDQE LQHIRNSLPD SVQIRRVEER
110 120 130 140 150
LSALGNVTTC NDYVALVHPD LDRETEEILA DVLKVEVFRQ TVADQVLVGS
160 170 180 190 200
YCVFSNQGGL VHPKTSIEDQ DELSSLLQVP LVAGTVNRGS EVIAAGMVVN
210 220 230 240
DWCAFCGLDT TSTELSVVES VFKLNEAKPS TIATSMRDSL IDSLT
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK003447 mRNA Translation: BAB22796.1 AK007594 mRNA Translation: BAB25125.1 AK012218 mRNA Translation: BAB28105.1 AK088707 mRNA Translation: BAC40517.1 AK134364 mRNA Translation: BAE22116.1 AK150400 mRNA Translation: BAE29526.1 AK150720 mRNA Translation: BAE29798.1 AK151089 mRNA Translation: BAE30102.1 AK151102 mRNA Translation: BAE30112.1 AK151644 mRNA Translation: BAE30574.1 AK152093 mRNA Translation: BAE30941.1 AK159157 mRNA Translation: BAE34862.1 AK165946 mRNA Translation: BAE38478.1 AK167422 mRNA Translation: BAE39510.1 AK168257 mRNA Translation: BAE40205.1 |
RefSeqi | NP_034709.1, NM_010579.2 |
Genome annotation databases
GeneIDi | 16418 |
KEGGi | mmu:16418 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK003447 mRNA Translation: BAB22796.1 AK007594 mRNA Translation: BAB25125.1 AK012218 mRNA Translation: BAB28105.1 AK088707 mRNA Translation: BAC40517.1 AK134364 mRNA Translation: BAE22116.1 AK150400 mRNA Translation: BAE29526.1 AK150720 mRNA Translation: BAE29798.1 AK151089 mRNA Translation: BAE30102.1 AK151102 mRNA Translation: BAE30112.1 AK151644 mRNA Translation: BAE30574.1 AK152093 mRNA Translation: BAE30941.1 AK159157 mRNA Translation: BAE34862.1 AK165946 mRNA Translation: BAE38478.1 AK167422 mRNA Translation: BAE39510.1 AK168257 mRNA Translation: BAE40205.1 |
RefSeqi | NP_034709.1, NM_010579.2 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protocols and materials databases
Antibodypediai | 35156, 257 antibodies from 35 providers |
DNASUi | 16418 |
Genome annotation databases
GeneIDi | 16418 |
KEGGi | mmu:16418 |
Organism-specific databases
CTDi | 3692 |
MGIi | MGI:1196288, Eif6 |
VEuPathDBi | HostDB:ENSMUSG00000027613 |
Phylogenomic databases
HOGENOMi | CLU_071894_0_0_1 |
OMAi | GEDTTGP |
OrthoDBi | 1157302at2759 |
Miscellaneous databases
BioGRID-ORCSi | 16418, 34 hits in 78 CRISPR screens |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000027613, Expressed in adult mammalian kidney and 319 other tissues |
Family and domain databases
CDDi | cd00527, IF6, 1 hit |
HAMAPi | MF_00032, eIF_6, 1 hit |
InterProi | View protein in InterPro IPR002769, eIF6 |
PANTHERi | PTHR10784, PTHR10784, 1 hit |
Pfami | View protein in Pfam PF01912, eIF-6, 1 hit |
PIRSFi | PIRSF006413, IF-6, 1 hit |
SMARTi | View protein in SMART SM00654, eIF6, 1 hit |
TIGRFAMsi | TIGR00323, eIF-6, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | Q545K4_MOUSE | |
Accessioni | Q545K4Primary (citable) accession number: Q545K4 | |
Entry historyi | Integrated into UniProtKB/TrEMBL: | May 24, 2005 |
Last sequence update: | May 24, 2005 | |
Last modified: | May 25, 2022 | |
This is version 145 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Unreviewed (UniProtKB/TrEMBL) |