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UniProtKB - Q544T5 (Q544T5_MOUSE)
Protein
Dihydrofolate reductase
Gene
Dhfr
Organism
Mus musculus (Mouse)
Status
Functioni
Catalytic activityi
- EC:1.5.1.3ARBA annotation
: tetrahydrofolate biosynthesis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate.ARBA annotation This subpathway is part of the pathway tetrahydrofolate biosynthesis, which is itself part of Cofactor biosynthesis.View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate, the pathway tetrahydrofolate biosynthesis and in Cofactor biosynthesis.
GO - Molecular functioni
- dihydrofolate reductase activity Source: UniProtKB-EC
- dihydrofolic acid binding Source: Ensembl
- NADP binding Source: Ensembl
GO - Biological processi
- axon regeneration Source: Ensembl
- dihydrofolate metabolic process Source: Ensembl
- folic acid metabolic process Source: Ensembl
- glycine biosynthetic process Source: InterPro
- one-carbon metabolic process Source: UniProtKB-KW
- response to methotrexate Source: UniProtKB-KW
- response to nicotine Source: Ensembl
- tetrahydrofolate biosynthetic process Source: UniProtKB-UniPathway
Keywordsi
Molecular function | OxidoreductaseARBA annotation |
Biological process | Methotrexate resistanceARBA annotation, One-carbon metabolismARBA annotation |
Ligand | NADPARBA annotation |
Enzyme and pathway databases
UniPathwayi | UPA00077;UER00158 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:DhfrImported |
Organismi | Mus musculus (Mouse)Imported |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Organism-specific databases
MGIi | MGI:94890, Dhfr |
VEuPathDBi | HostDB:ENSMUSG00000021707 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000021707, Expressed in telencephalon and 302 other tissues |
Structurei
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 4 – 185 | DHFRInterPro annotationAdd BLAST | 182 |
Sequence similaritiesi
Belongs to the dihydrofolate reductase family.UniRule annotation
Phylogenomic databases
HOGENOMi | CLU_043966_2_3_1 |
OMAi | RDNQLPW |
OrthoDBi | 1489621at2759 |
Family and domain databases
CDDi | cd00209, DHFR, 1 hit |
Gene3Di | 3.40.430.10, 1 hit |
InterProi | View protein in InterPro IPR012259, DHFR IPR024072, DHFR-like_dom_sf IPR017925, DHFR_CS IPR001796, DHFR_dom |
PANTHERi | PTHR48069, PTHR48069, 1 hit |
Pfami | View protein in Pfam PF00186, DHFR_1, 1 hit |
SUPFAMi | SSF53597, SSF53597, 1 hit |
PROSITEi | View protein in PROSITE PS00075, DHFR_1, 1 hit PS51330, DHFR_2, 1 hit |
i Sequence
Sequence statusi: Complete.
Q544T5-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MVRPLNCIVA VSQNMGIGKN GDLPWPPLRN EFKYFQRMTT TSSVEGKQNL
60 70 80 90 100
VIMGRKTWFS IPEKNRPLKD RINIVLSREL KEPPRGAHFL AKSLDDALRL
110 120 130 140 150
IEQPELASKV DMVWIVGGSS VYQEAMNQPG HLRLFVTRIM QEFESDTFFP
160 170 180
EIDLGKYKLL PEYPGVLSEV QEEKGIKYKF EVYEKKD
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK031241 mRNA Translation: BAC27315.1 AK136212 mRNA Translation: BAE22879.1 |
RefSeqi | NP_034179.1, NM_010049.3 |
Genome annotation databases
GeneIDi | 13361 |
KEGGi | mmu:13361 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK031241 mRNA Translation: BAC27315.1 AK136212 mRNA Translation: BAE22879.1 |
RefSeqi | NP_034179.1, NM_010049.3 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protocols and materials databases
DNASUi | 13361 |
Genome annotation databases
GeneIDi | 13361 |
KEGGi | mmu:13361 |
Organism-specific databases
CTDi | 1719 |
MGIi | MGI:94890, Dhfr |
VEuPathDBi | HostDB:ENSMUSG00000021707 |
Phylogenomic databases
HOGENOMi | CLU_043966_2_3_1 |
OMAi | RDNQLPW |
OrthoDBi | 1489621at2759 |
Enzyme and pathway databases
UniPathwayi | UPA00077;UER00158 |
Miscellaneous databases
BioGRID-ORCSi | 13361, 24 hits in 64 CRISPR screens |
ChiTaRSi | Dhfr, mouse |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000021707, Expressed in telencephalon and 302 other tissues |
Family and domain databases
CDDi | cd00209, DHFR, 1 hit |
Gene3Di | 3.40.430.10, 1 hit |
InterProi | View protein in InterPro IPR012259, DHFR IPR024072, DHFR-like_dom_sf IPR017925, DHFR_CS IPR001796, DHFR_dom |
PANTHERi | PTHR48069, PTHR48069, 1 hit |
Pfami | View protein in Pfam PF00186, DHFR_1, 1 hit |
SUPFAMi | SSF53597, SSF53597, 1 hit |
PROSITEi | View protein in PROSITE PS00075, DHFR_1, 1 hit PS51330, DHFR_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | Q544T5_MOUSE | |
Accessioni | Q544T5Primary (citable) accession number: Q544T5 | |
Entry historyi | Integrated into UniProtKB/TrEMBL: | May 24, 2005 |
Last sequence update: | May 24, 2005 | |
Last modified: | January 19, 2022 | |
This is version 136 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Unreviewed (UniProtKB/TrEMBL) |