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UniProtKB - Q53HL2 (BOREA_HUMAN)

Protein

Borealin

Gene

CDCA8

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Major effector of the TTK kinase in the control of attachment-error-correction and chromosome alignment.4 Publications

Miscellaneous

Cells lacking CDCA8 display a slight decrease in histone H3 'Ser-10' phosphorylation, suggesting that the CPC complex mediates phosphorylation of 'Ser-10' of histone H3.

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • chromosome organization Source: UniProtKB
  • mitotic metaphase plate congression Source: UniProtKB
  • mitotic sister chromatid segregation Source: GO_Central
View the complete GO annotation on QuickGO ...

Keywordsi

Biological processCell cycle, Cell division, Mitosis

Enzyme and pathway databases

ReactomeiR-HSA-141444 Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal
R-HSA-2467813 Separation of Sister Chromatids
R-HSA-2500257 Resolution of Sister Chromatid Cohesion
R-HSA-4615885 SUMOylation of DNA replication proteins
R-HSA-5663220 RHO GTPases Activate Formins
R-HSA-68877 Mitotic Prometaphase
SIGNORiQ53HL2

Names & Taxonomyi

Protein namesi
Recommended name:
Borealin
Alternative name(s):
Cell division cycle-associated protein 8
Dasra-B
Short name:
hDasra-B
Pluripotent embryonic stem cell-related gene 3 protein
Gene namesi
Name:CDCA8
Synonyms:PESCRG3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiHostDB:ENSG00000134690.10
HGNCiHGNC:14629 CDCA8
MIMi609977 gene
neXtProtiNX_Q53HL2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Centromere, Chromosome, Cytoplasm, Cytoskeleton, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi17R → E: Loss of localization to the central spindle and midbody in anaphase or cytokinesis; when associated with E-19 and E-20. 1 Publication1
Mutagenesisi19R → E: Loss of localization to the central spindle and midbody in anaphase or cytokinesis; when associated with E-17 and E-20. 1 Publication1
Mutagenesisi20K → E: Loss of localization to the central spindle and midbody in anaphase or cytokinesis; when associated with E-17 and E-19. 1 Publication1
Mutagenesisi26K → R: Fails to exhibit normal localization to the nucleolus in interphase depleted cells. 1 Publication1
Mutagenesisi35R → E: Loss of binding to INCENP; when associated with Y-46. 1 Publication1
Mutagenesisi46L → Y: Loss of binding to INCENP; when associated with E-35. 1 Publication1
Mutagenesisi70W → E: Loss of binding to BIRC5; when associated with E-74. 1 Publication1
Mutagenesisi74F → E: Loss of binding to BIRC5; when associated with E-70. 1 Publication1
Mutagenesisi88T → A: Decrease in AURKB activity and almost no phosphorylation by TTK; when associated with A-94; A-169 and A-230. 1 Publication1
Mutagenesisi94T → A: Decrease in AURKB activity and almost no phosphorylation by TTK; when associated with A-88; A-169 and A-230. 1 Publication1
Mutagenesisi106T → A: Decreases interaction with SOG1 and SOG2, abolishes localization to centromeres in prometaphase; when associated with A-171, A-185, A-189, A-199, A-204 and A-219. 1 Publication1
Mutagenesisi165S → A: Results in reduction but not abolition of phosphorylation. 1 Publication1
Mutagenesisi169T → A: Decrease in AURKB activity and almost no phosphorylation by TTK; when associated with A-88; A-94 and A-230. 1 Publication1
Mutagenesisi171T → A: Decreases interaction with SOG1 and SOG2, abolishes localization to centromeres in prometaphase; when associated with A-106, A-185, A-189, A-199, A-204 and A-219. 1 Publication1
Mutagenesisi185T → A: Decreases interaction with SOG1 and SOG2, abolishes localization to centromeres in prometaphase; when associated with A-106, A-171, A-189, A-199, A-204 and A-219. 1 Publication1
Mutagenesisi189T → A: Decreases interaction with SOG1 and SOG2, abolishes localization to centromeres in prometaphase; when associated with A-106, A-171, A-185, A-199, A-204 and A-219. 1 Publication1
Mutagenesisi199T → A: Decreases interaction with SOG1 and SOG2, abolishes localization to centromeres in prometaphase; when associated with A-106, A-171, A-185, A-189, A-204, A-219. 1 Publication1
Mutagenesisi204T → A: Decreases interaction with SOG1 and SOG2, abolishes localization to centromeres in prometaphase; when associated with A-106, A-171, A-185, A-189, A-199 and A-219. 1 Publication1
Mutagenesisi219S → A: Decreases interaction with SOG1 and SOG2, abolishes localization to centromeres in prometaphase; when associated with A-106, A-171, A-185, A-189, A-199 and A-204. 1 Publication1
Mutagenesisi219S → D or K: No effect on the structure. 1
Mutagenesisi230T → A: Decrease in AURKB activity and dimer disruption. Decrease in AURKB activity and almost no phosphorylation by TTK; when associated with A-88; A-94 and A-230. 1 Publication1
Mutagenesisi230T → D or K: Substantial loss of structure. 1 Publication1
Mutagenesisi230T → V: Decrease in AURKB activity and no effect on the structure. 1 Publication1

Organism-specific databases

DisGeNETi55143
OpenTargetsiENSG00000134690
PharmGKBiPA26281

Polymorphism and mutation databases

BioMutaiCDCA8
DMDMi110832774

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002470751 – 280BorealinAdd BLAST280

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei88Phosphothreonine; by TTK1 Publication1
Modified residuei94Phosphothreonine; by TTK1 Publication1
Modified residuei106PhosphothreonineCombined sources1
Modified residuei110PhosphoserineCombined sources1
Cross-linki135Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei165Phosphoserine; by AURKB1 Publication1
Modified residuei169Phosphothreonine; by TTK1 Publication1
Modified residuei189PhosphothreonineCombined sources1
Modified residuei204PhosphothreonineCombined sources1
Modified residuei219PhosphoserineCombined sources1
Modified residuei224PhosphoserineCombined sources1
Modified residuei230Phosphothreonine; by TTK1 Publication1
Modified residuei238Phosphoserine1 Publication1
Modified residuei244PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated by TTK, essentially at Thr-88, Thr94, Thr-169 and Thr-230. Phosphorylation (probably by CDK1) promotes targeting of the CPC to centromeric DNA.3 Publications
Sumoylated by UBE2I and RANBP2. Desumoylated by SENP3 through the removal of SUMO2 and SUMO3.1 Publication

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ53HL2
MaxQBiQ53HL2
PaxDbiQ53HL2
PeptideAtlasiQ53HL2
PRIDEiQ53HL2
ProteomicsDBi62507

PTM databases

iPTMnetiQ53HL2
PhosphoSitePlusiQ53HL2

Expressioni

Developmental stagei

Cell-cycle regulated. Increases during G2/M phase and then reduces after exit from M phase.1 Publication

Gene expression databases

BgeeiENSG00000134690 Expressed in 126 organ(s), highest expression level in oocyte
CleanExiHS_CDCA8
GenevisibleiQ53HL2 HS

Organism-specific databases

HPAiCAB040294
HPA028120
HPA028258
HPA028783

Interactioni

Subunit structurei

May form homooligomers and homodimers. Component of the chromosomal passenger complex (CPC) composed of at least BIRC5/survivin, CDCA8/borealin, INCENP, AURKB or AURKC; in the complex forms a triple-helix bundle-based subcomplex with INCENP and BIRC5 (PubMed:17956729). Interacts with SENP3, UBE2I and RANBP2. Interacts (phosphorylated) with SGO1 and SGO2; the association is dependent on CDK1.9 Publications

Binary interactionsi

Protein-protein interaction databases

BioGridi120446, 67 interactors
ComplexPortaliCPX-116 Chromosomal passenger complex
CORUMiQ53HL2
DIPiDIP-37995N
IntActiQ53HL2, 26 interactors
MINTiQ53HL2
STRINGi9606.ENSP00000316121

Structurei

Secondary structure

1280
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ53HL2
SMRiQ53HL2
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ53HL2

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 140Required for interaction with SENP31 PublicationAdd BLAST140
Regioni1 – 88Required for centromere localizationAdd BLAST88
Regioni1 – 58Required for interaction with INCENP1 PublicationAdd BLAST58
Regioni10 – 109Required to form a minimal CPC core complex that localizes to the central spindle and midbody and properly executes the role of the CPC during cytokinesisAdd BLAST100
Regioni20 – 78Required for interaction with INCENP and BIRC51 PublicationAdd BLAST59

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi125 – 133Poly-Glu9

Domaini

The C-terminal region (aa 207-280) represents the dimerization motif.

Sequence similaritiesi

Belongs to the borealin family.Curated

Phylogenomic databases

eggNOGiENOG410IICJ Eukaryota
ENOG4111N8R LUCA
GeneTreeiENSGT00390000011115
HOGENOMiHOG000261628
HOVERGENiHBG080103
InParanoidiQ53HL2
KOiK11514
OMAiMNWLEYF
OrthoDBiEOG091G167R
PhylomeDBiQ53HL2
TreeFamiTF101077

Family and domain databases

InterProiView protein in InterPro
IPR018851 Borealin_N
IPR018867 Cell_div_borealin
PfamiView protein in Pfam
PF10512 Borealin, 1 hit
PF10444 Nbl1_Borealin_N, 1 hit

Sequencei

Sequence statusi: Complete.

Q53HL2-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAPRKGSSRV AKTNSLRRRK LASFLKDFDR EVEIRIKQIE SDRQNLLKEV 
        60         70         80         90        100
DNLYNIEILR LPKALREMNW LDYFALGGNK QALEEAATAD LDITEINKLT 
       110        120        130        140        150
AEAIQTPLKS AKTRKVIQVD EMIVEEEEEE ENERKNLQTA RVKRCPPSKK 
       160        170        180        190        200
RTQSIQGKGK GKRSSRANTV TPAVGRLEVS MVKPTPGLTP RFDSRVFKTP 
       210        220        230        240        250
GLRTPAAGER IYNISGNGSP LADSKEIFLT VPVGGGESLR LLASDLQRHS 
       260        270        280
IAQLDPEALG NIKKLSNRLA QICSSIRTHK
Length:280
Mass (Da):31,323
Last modified:July 25, 2006 - v2
Checksum:i519978A7C295C571
GO

Sequence cautioni

The sequence BG354581 differs from that shown. Reason: Frameshift at positions 123 and 200.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti155I → M in BAD96288 (Ref. 4) Curated1
Sequence conflicti213N → D in BAD96269 (Ref. 4) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_02706312K → N1 PublicationCorresponds to variant dbSNP:rs17851453Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BG354581 mRNA No translation available.
AY508815 mRNA Translation: AAR91699.1
AK001330 mRNA Translation: BAA91629.1
AK022104 mRNA Translation: BAB13961.1
AK022606 mRNA Translation: BAB14125.1
AK222549 mRNA Translation: BAD96269.1
AK222568 mRNA Translation: BAD96288.1
CH471059 Genomic DNA Translation: EAX07324.1
CH471059 Genomic DNA Translation: EAX07325.1
BC000703 mRNA Translation: AAH00703.1
BC001651 mRNA Translation: AAH01651.1
BC016944 mRNA Translation: AAH16944.1
BC008079 mRNA Translation: AAH08079.1
CCDSiCCDS424.1
RefSeqiNP_001243804.1, NM_001256875.1
NP_060571.1, NM_018101.3
UniGeneiHs.524571

Genome annotation databases

EnsembliENST00000327331; ENSP00000316121; ENSG00000134690
ENST00000373055; ENSP00000362146; ENSG00000134690
GeneIDi55143
KEGGihsa:55143
UCSCiuc001cbr.5 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BG354581 mRNA No translation available.
AY508815 mRNA Translation: AAR91699.1
AK001330 mRNA Translation: BAA91629.1
AK022104 mRNA Translation: BAB13961.1
AK022606 mRNA Translation: BAB14125.1
AK222549 mRNA Translation: BAD96269.1
AK222568 mRNA Translation: BAD96288.1
CH471059 Genomic DNA Translation: EAX07324.1
CH471059 Genomic DNA Translation: EAX07325.1
BC000703 mRNA Translation: AAH00703.1
BC001651 mRNA Translation: AAH01651.1
BC016944 mRNA Translation: AAH16944.1
BC008079 mRNA Translation: AAH08079.1
CCDSiCCDS424.1
RefSeqiNP_001243804.1, NM_001256875.1
NP_060571.1, NM_018101.3
UniGeneiHs.524571

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2KDDNMR-A/B207-280[»]
2QFAX-ray1.40B15-76[»]
2RAWX-ray2.40B20-78[»]
2RAXX-ray3.30B/F/Y20-78[»]
ProteinModelPortaliQ53HL2
SMRiQ53HL2
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120446, 67 interactors
ComplexPortaliCPX-116 Chromosomal passenger complex
CORUMiQ53HL2
DIPiDIP-37995N
IntActiQ53HL2, 26 interactors
MINTiQ53HL2
STRINGi9606.ENSP00000316121

PTM databases

iPTMnetiQ53HL2
PhosphoSitePlusiQ53HL2

Polymorphism and mutation databases

BioMutaiCDCA8
DMDMi110832774

Proteomic databases

EPDiQ53HL2
MaxQBiQ53HL2
PaxDbiQ53HL2
PeptideAtlasiQ53HL2
PRIDEiQ53HL2
ProteomicsDBi62507

Protocols and materials databases

DNASUi55143
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000327331; ENSP00000316121; ENSG00000134690
ENST00000373055; ENSP00000362146; ENSG00000134690
GeneIDi55143
KEGGihsa:55143
UCSCiuc001cbr.5 human

Organism-specific databases

CTDi55143
DisGeNETi55143
EuPathDBiHostDB:ENSG00000134690.10
GeneCardsiCDCA8
HGNCiHGNC:14629 CDCA8
HPAiCAB040294
HPA028120
HPA028258
HPA028783
MIMi609977 gene
neXtProtiNX_Q53HL2
OpenTargetsiENSG00000134690
PharmGKBiPA26281
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IICJ Eukaryota
ENOG4111N8R LUCA
GeneTreeiENSGT00390000011115
HOGENOMiHOG000261628
HOVERGENiHBG080103
InParanoidiQ53HL2
KOiK11514
OMAiMNWLEYF
OrthoDBiEOG091G167R
PhylomeDBiQ53HL2
TreeFamiTF101077

Enzyme and pathway databases

ReactomeiR-HSA-141444 Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal
R-HSA-2467813 Separation of Sister Chromatids
R-HSA-2500257 Resolution of Sister Chromatid Cohesion
R-HSA-4615885 SUMOylation of DNA replication proteins
R-HSA-5663220 RHO GTPases Activate Formins
R-HSA-68877 Mitotic Prometaphase
SIGNORiQ53HL2

Miscellaneous databases

ChiTaRSiCDCA8 human
EvolutionaryTraceiQ53HL2
GeneWikiiCDCA8
GenomeRNAii55143
PROiPR:Q53HL2
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000134690 Expressed in 126 organ(s), highest expression level in oocyte
CleanExiHS_CDCA8
GenevisibleiQ53HL2 HS

Family and domain databases

InterProiView protein in InterPro
IPR018851 Borealin_N
IPR018867 Cell_div_borealin
PfamiView protein in Pfam
PF10512 Borealin, 1 hit
PF10444 Nbl1_Borealin_N, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiBOREA_HUMAN
AccessioniPrimary (citable) accession number: Q53HL2
Secondary accession number(s): D3DPT4
, Q53HN1, Q96AM3, Q9NVW5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: July 25, 2006
Last modified: November 7, 2018
This is version 130 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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