UniProtKB - Q53HL2 (BOREA_HUMAN)
Protein
Borealin
Gene
CDCA8
Organism
Homo sapiens (Human)
Status
Functioni
Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Major effector of the TTK kinase in the control of attachment-error-correction and chromosome alignment.4 Publications
Miscellaneous
Cells lacking CDCA8 display a slight decrease in histone H3 'Ser-10' phosphorylation, suggesting that the CPC complex mediates phosphorylation of 'Ser-10' of histone H3.
GO - Biological processi
- cell division Source: UniProtKB-KW
- chromosome organization Source: UniProtKB
- mitotic metaphase plate congression Source: UniProtKB
- mitotic sister chromatid segregation Source: GO_Central
Keywordsi
Biological process | Cell cycle, Cell division, Mitosis |
Enzyme and pathway databases
PathwayCommonsi | Q53HL2 |
Reactomei | R-HSA-141444, Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal R-HSA-2467813, Separation of Sister Chromatids R-HSA-2500257, Resolution of Sister Chromatid Cohesion R-HSA-4615885, SUMOylation of DNA replication proteins R-HSA-5663220, RHO GTPases Activate Formins R-HSA-68877, Mitotic Prometaphase R-HSA-9648025, EML4 and NUDC in mitotic spindle formation |
SIGNORi | Q53HL2 |
Names & Taxonomyi
Protein namesi | Recommended name: BorealinAlternative name(s): Cell division cycle-associated protein 8 Dasra-B Short name: hDasra-B Pluripotent embryonic stem cell-related gene 3 protein |
Gene namesi | Name:CDCA8 Synonyms:PESCRG3 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000134690.10 |
HGNCi | HGNC:14629, CDCA8 |
MIMi | 609977, gene |
neXtProti | NX_Q53HL2 |
Subcellular locationi
Nucleus
- nucleolus 1 Publication
Cytoskeleton
- spindle 1 Publication
Other locations
- Cytoplasm 1 Publication
- centromere 1 Publication
Note: Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalizes with SENP3 in the nucleolus in interphase cells.1 Publication
Cytoskeleton
- chromosome passenger complex Source: UniProtKB
- spindle midzone Source: GO_Central
Cytosol
- cytosol Source: Reactome
Extracellular region or secreted
- intercellular bridge Source: HPA
Nucleus
- nucleolus Source: HPA
- nucleoplasm Source: HPA
Other locations
- chromocenter Source: Ensembl
- chromosome, centromeric region Source: UniProtKB
- midbody Source: FlyBase
- protein-containing complex Source: UniProtKB
Keywords - Cellular componenti
Centromere, Chromosome, Cytoplasm, Cytoskeleton, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 17 | R → E: Loss of localization to the central spindle and midbody in anaphase or cytokinesis; when associated with E-19 and E-20. 1 Publication | 1 | |
Mutagenesisi | 19 | R → E: Loss of localization to the central spindle and midbody in anaphase or cytokinesis; when associated with E-17 and E-20. 1 Publication | 1 | |
Mutagenesisi | 20 | K → E: Loss of localization to the central spindle and midbody in anaphase or cytokinesis; when associated with E-17 and E-19. 1 Publication | 1 | |
Mutagenesisi | 26 | K → R: Fails to exhibit normal localization to the nucleolus in interphase depleted cells. 1 Publication | 1 | |
Mutagenesisi | 35 | R → E: Loss of binding to INCENP; when associated with Y-46. 1 Publication | 1 | |
Mutagenesisi | 46 | L → Y: Loss of binding to INCENP; when associated with E-35. 1 Publication | 1 | |
Mutagenesisi | 70 | W → E: Loss of binding to BIRC5; when associated with E-74. 1 Publication | 1 | |
Mutagenesisi | 74 | F → E: Loss of binding to BIRC5; when associated with E-70. 1 Publication | 1 | |
Mutagenesisi | 88 | T → A: Decrease in AURKB activity and almost no phosphorylation by TTK; when associated with A-94; A-169 and A-230. 1 Publication | 1 | |
Mutagenesisi | 94 | T → A: Decrease in AURKB activity and almost no phosphorylation by TTK; when associated with A-88; A-169 and A-230. 1 Publication | 1 | |
Mutagenesisi | 106 | T → A: Decreases interaction with SOG1 and SOG2, abolishes localization to centromeres in prometaphase; when associated with A-171, A-185, A-189, A-199, A-204 and A-219. 1 Publication | 1 | |
Mutagenesisi | 165 | S → A: Results in reduction but not abolition of phosphorylation. 1 Publication | 1 | |
Mutagenesisi | 169 | T → A: Decrease in AURKB activity and almost no phosphorylation by TTK; when associated with A-88; A-94 and A-230. 1 Publication | 1 | |
Mutagenesisi | 171 | T → A: Decreases interaction with SOG1 and SOG2, abolishes localization to centromeres in prometaphase; when associated with A-106, A-185, A-189, A-199, A-204 and A-219. 1 Publication | 1 | |
Mutagenesisi | 185 | T → A: Decreases interaction with SOG1 and SOG2, abolishes localization to centromeres in prometaphase; when associated with A-106, A-171, A-189, A-199, A-204 and A-219. 1 Publication | 1 | |
Mutagenesisi | 189 | T → A: Decreases interaction with SOG1 and SOG2, abolishes localization to centromeres in prometaphase; when associated with A-106, A-171, A-185, A-199, A-204 and A-219. 1 Publication | 1 | |
Mutagenesisi | 199 | T → A: Decreases interaction with SOG1 and SOG2, abolishes localization to centromeres in prometaphase; when associated with A-106, A-171, A-185, A-189, A-204, A-219. 1 Publication | 1 | |
Mutagenesisi | 204 | T → A: Decreases interaction with SOG1 and SOG2, abolishes localization to centromeres in prometaphase; when associated with A-106, A-171, A-185, A-189, A-199 and A-219. 1 Publication | 1 | |
Mutagenesisi | 219 | S → A: Decreases interaction with SOG1 and SOG2, abolishes localization to centromeres in prometaphase; when associated with A-106, A-171, A-185, A-189, A-199 and A-204. 1 Publication | 1 | |
Mutagenesisi | 219 | S → D or K: No effect on the structure. | 1 | |
Mutagenesisi | 230 | T → A: Decrease in AURKB activity and dimer disruption. Decrease in AURKB activity and almost no phosphorylation by TTK; when associated with A-88; A-94 and A-230. 1 Publication | 1 | |
Mutagenesisi | 230 | T → D or K: Substantial loss of structure. 1 Publication | 1 | |
Mutagenesisi | 230 | T → V: Decrease in AURKB activity and no effect on the structure. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 55143 |
OpenTargetsi | ENSG00000134690 |
PharmGKBi | PA26281 |
Miscellaneous databases
Pharosi | Q53HL2, Tbio |
Polymorphism and mutation databases
BioMutai | CDCA8 |
DMDMi | 110832774 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000247075 | 1 – 280 | BorealinAdd BLAST | 280 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 88 | Phosphothreonine; by TTK1 Publication | 1 | |
Modified residuei | 94 | Phosphothreonine; by TTK1 Publication | 1 | |
Modified residuei | 106 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 110 | PhosphoserineCombined sources | 1 | |
Cross-linki | 135 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Modified residuei | 165 | Phosphoserine; by AURKB1 Publication | 1 | |
Modified residuei | 169 | Phosphothreonine; by TTK1 Publication | 1 | |
Modified residuei | 189 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 204 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 219 | PhosphoserineCombined sources | 1 | |
Modified residuei | 224 | PhosphoserineCombined sources | 1 | |
Modified residuei | 230 | Phosphothreonine; by TTK1 Publication | 1 | |
Modified residuei | 238 | Phosphoserine1 Publication | 1 | |
Modified residuei | 244 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Phosphorylated by TTK, essentially at Thr-88, Thr94, Thr-169 and Thr-230. Phosphorylation (probably by CDK1) promotes targeting of the CPC to centromeric DNA.3 Publications
Sumoylated by UBE2I and RANBP2. Desumoylated by SENP3 through the removal of SUMO2 and SUMO3.1 Publication
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q53HL2 |
jPOSTi | Q53HL2 |
MassIVEi | Q53HL2 |
MaxQBi | Q53HL2 |
PaxDbi | Q53HL2 |
PeptideAtlasi | Q53HL2 |
PRIDEi | Q53HL2 |
ProteomicsDBi | 62507 |
PTM databases
iPTMneti | Q53HL2 |
PhosphoSitePlusi | Q53HL2 |
Expressioni
Developmental stagei
Cell-cycle regulated. Increases during G2/M phase and then reduces after exit from M phase.1 Publication
Gene expression databases
Bgeei | ENSG00000134690, Expressed in oocyte and 146 other tissues |
Genevisiblei | Q53HL2, HS |
Organism-specific databases
HPAi | ENSG00000134690, Group enriched (bone marrow, lymphoid tissue, testis) |
Interactioni
Subunit structurei
May form homooligomers and homodimers.
Component of the chromosomal passenger complex (CPC) composed of at least BIRC5/survivin, CDCA8/borealin, INCENP, AURKB or AURKC; in the complex forms a triple-helix bundle-based subcomplex with INCENP and BIRC5 (PubMed:17956729).
Interacts with SENP3, UBE2I and RANBP2.
Interacts (phosphorylated) with SGO1 and SGO2; the association is dependent on CDK1.
9 PublicationsBinary interactionsi
Hide detailsQ53HL2
Protein-protein interaction databases
BioGRIDi | 120446, 82 interactors |
ComplexPortali | CPX-116, Chromosomal passenger complex |
CORUMi | Q53HL2 |
DIPi | DIP-37995N |
IntActi | Q53HL2, 53 interactors |
MINTi | Q53HL2 |
STRINGi | 9606.ENSP00000362146 |
Miscellaneous databases
RNActi | Q53HL2, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | Q53HL2 |
SMRi | Q53HL2 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q53HL2 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 140 | Required for interaction with SENP31 PublicationAdd BLAST | 140 | |
Regioni | 1 – 88 | Required for centromere localizationAdd BLAST | 88 | |
Regioni | 1 – 58 | Required for interaction with INCENP1 PublicationAdd BLAST | 58 | |
Regioni | 10 – 109 | Required to form a minimal CPC core complex that localizes to the central spindle and midbody and properly executes the role of the CPC during cytokinesisAdd BLAST | 100 | |
Regioni | 20 – 78 | Required for interaction with INCENP and BIRC51 PublicationAdd BLAST | 59 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 125 – 133 | Poly-Glu | 9 |
Domaini
The C-terminal region (aa 207-280) represents the dimerization motif.
Sequence similaritiesi
Belongs to the borealin family.Curated
Phylogenomic databases
eggNOGi | ENOG502QS4S, Eukaryota |
GeneTreei | ENSGT00390000011115 |
HOGENOMi | CLU_074128_0_0_1 |
InParanoidi | Q53HL2 |
OMAi | PLITPKF |
OrthoDBi | 1587028at2759 |
PhylomeDBi | Q53HL2 |
TreeFami | TF101077 |
Family and domain databases
IDEALi | IID00215 |
InterProi | View protein in InterPro IPR018851, Borealin_N IPR018867, Cell_div_borealin |
Pfami | View protein in Pfam PF10512, Borealin, 1 hit PF10444, Nbl1_Borealin_N, 1 hit |
i Sequence
Sequence statusi: Complete.
Q53HL2-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAPRKGSSRV AKTNSLRRRK LASFLKDFDR EVEIRIKQIE SDRQNLLKEV
60 70 80 90 100
DNLYNIEILR LPKALREMNW LDYFALGGNK QALEEAATAD LDITEINKLT
110 120 130 140 150
AEAIQTPLKS AKTRKVIQVD EMIVEEEEEE ENERKNLQTA RVKRCPPSKK
160 170 180 190 200
RTQSIQGKGK GKRSSRANTV TPAVGRLEVS MVKPTPGLTP RFDSRVFKTP
210 220 230 240 250
GLRTPAAGER IYNISGNGSP LADSKEIFLT VPVGGGESLR LLASDLQRHS
260 270 280
IAQLDPEALG NIKKLSNRLA QICSSIRTHK
Sequence cautioni
The sequence BG354581 differs from that shown. Reason: Frameshift.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 155 | I → M in BAD96288 (Ref. 4) Curated | 1 | |
Sequence conflicti | 213 | N → D in BAD96269 (Ref. 4) Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_027063 | 12 | K → N1 PublicationCorresponds to variant dbSNP:rs17851453Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BG354581 mRNA No translation available. AY508815 mRNA Translation: AAR91699.1 AK001330 mRNA Translation: BAA91629.1 AK022104 mRNA Translation: BAB13961.1 AK022606 mRNA Translation: BAB14125.1 AK222549 mRNA Translation: BAD96269.1 AK222568 mRNA Translation: BAD96288.1 CH471059 Genomic DNA Translation: EAX07324.1 CH471059 Genomic DNA Translation: EAX07325.1 BC000703 mRNA Translation: AAH00703.1 BC001651 mRNA Translation: AAH01651.1 BC016944 mRNA Translation: AAH16944.1 BC008079 mRNA Translation: AAH08079.1 |
CCDSi | CCDS424.1 |
RefSeqi | NP_001243804.1, NM_001256875.1 NP_060571.1, NM_018101.3 |
Genome annotation databases
Ensembli | ENST00000327331; ENSP00000316121; ENSG00000134690 ENST00000373055; ENSP00000362146; ENSG00000134690 |
GeneIDi | 55143 |
KEGGi | hsa:55143 |
UCSCi | uc001cbr.5, human |
Keywords - Coding sequence diversityi
PolymorphismSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BG354581 mRNA No translation available. AY508815 mRNA Translation: AAR91699.1 AK001330 mRNA Translation: BAA91629.1 AK022104 mRNA Translation: BAB13961.1 AK022606 mRNA Translation: BAB14125.1 AK222549 mRNA Translation: BAD96269.1 AK222568 mRNA Translation: BAD96288.1 CH471059 Genomic DNA Translation: EAX07324.1 CH471059 Genomic DNA Translation: EAX07325.1 BC000703 mRNA Translation: AAH00703.1 BC001651 mRNA Translation: AAH01651.1 BC016944 mRNA Translation: AAH16944.1 BC008079 mRNA Translation: AAH08079.1 |
CCDSi | CCDS424.1 |
RefSeqi | NP_001243804.1, NM_001256875.1 NP_060571.1, NM_018101.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2KDD | NMR | - | A/B | 207-280 | [»] | |
2QFA | X-ray | 1.40 | B | 15-76 | [»] | |
2RAW | X-ray | 2.40 | B | 20-78 | [»] | |
2RAX | X-ray | 3.30 | B/F/Y | 20-78 | [»] | |
6YIE | X-ray | 3.49 | B/E | 10-109 | [»] | |
6YIF | X-ray | 1.81 | B | 10-76 | [»] | |
6YIH | X-ray | 2.55 | B | 10-76 | [»] | |
BMRBi | Q53HL2 | |||||
SMRi | Q53HL2 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 120446, 82 interactors |
ComplexPortali | CPX-116, Chromosomal passenger complex |
CORUMi | Q53HL2 |
DIPi | DIP-37995N |
IntActi | Q53HL2, 53 interactors |
MINTi | Q53HL2 |
STRINGi | 9606.ENSP00000362146 |
PTM databases
iPTMneti | Q53HL2 |
PhosphoSitePlusi | Q53HL2 |
Polymorphism and mutation databases
BioMutai | CDCA8 |
DMDMi | 110832774 |
Proteomic databases
EPDi | Q53HL2 |
jPOSTi | Q53HL2 |
MassIVEi | Q53HL2 |
MaxQBi | Q53HL2 |
PaxDbi | Q53HL2 |
PeptideAtlasi | Q53HL2 |
PRIDEi | Q53HL2 |
ProteomicsDBi | 62507 |
Protocols and materials databases
Antibodypediai | 31756, 454 antibodies |
CPTCi | Q53HL2, 1 antibody |
DNASUi | 55143 |
Genome annotation databases
Ensembli | ENST00000327331; ENSP00000316121; ENSG00000134690 ENST00000373055; ENSP00000362146; ENSG00000134690 |
GeneIDi | 55143 |
KEGGi | hsa:55143 |
UCSCi | uc001cbr.5, human |
Organism-specific databases
CTDi | 55143 |
DisGeNETi | 55143 |
EuPathDBi | HostDB:ENSG00000134690.10 |
GeneCardsi | CDCA8 |
HGNCi | HGNC:14629, CDCA8 |
HPAi | ENSG00000134690, Group enriched (bone marrow, lymphoid tissue, testis) |
MIMi | 609977, gene |
neXtProti | NX_Q53HL2 |
OpenTargetsi | ENSG00000134690 |
PharmGKBi | PA26281 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502QS4S, Eukaryota |
GeneTreei | ENSGT00390000011115 |
HOGENOMi | CLU_074128_0_0_1 |
InParanoidi | Q53HL2 |
OMAi | PLITPKF |
OrthoDBi | 1587028at2759 |
PhylomeDBi | Q53HL2 |
TreeFami | TF101077 |
Enzyme and pathway databases
PathwayCommonsi | Q53HL2 |
Reactomei | R-HSA-141444, Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal R-HSA-2467813, Separation of Sister Chromatids R-HSA-2500257, Resolution of Sister Chromatid Cohesion R-HSA-4615885, SUMOylation of DNA replication proteins R-HSA-5663220, RHO GTPases Activate Formins R-HSA-68877, Mitotic Prometaphase R-HSA-9648025, EML4 and NUDC in mitotic spindle formation |
SIGNORi | Q53HL2 |
Miscellaneous databases
BioGRID-ORCSi | 55143, 661 hits in 855 CRISPR screens |
ChiTaRSi | CDCA8, human |
EvolutionaryTracei | Q53HL2 |
GeneWikii | CDCA8 |
GenomeRNAii | 55143 |
Pharosi | Q53HL2, Tbio |
PROi | PR:Q53HL2 |
RNActi | Q53HL2, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000134690, Expressed in oocyte and 146 other tissues |
Genevisiblei | Q53HL2, HS |
Family and domain databases
IDEALi | IID00215 |
InterProi | View protein in InterPro IPR018851, Borealin_N IPR018867, Cell_div_borealin |
Pfami | View protein in Pfam PF10512, Borealin, 1 hit PF10444, Nbl1_Borealin_N, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | BOREA_HUMAN | |
Accessioni | Q53HL2Primary (citable) accession number: Q53HL2 Secondary accession number(s): D3DPT4 Q9NVW5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 25, 2006 |
Last sequence update: | July 25, 2006 | |
Last modified: | December 2, 2020 | |
This is version 144 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Human chromosome 1
Human chromosome 1: entries, gene names and cross-references to MIM - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations