Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

tRNA wybutosine-synthesizing protein 2 homolog

Gene

TRMT12

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

S-adenosyl-L-methionine-dependent transferase that acts as a component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA. Catalyzes the transfer of the alpha-amino-alpha-carboxypropyl (acp) group from S-adenosyl-L-methionine to the C-7 position of 4-demethylwyosine (imG-14) to produce wybutosine-86.1 Publication

Catalytic activityi

S-adenosyl-L-methionine + 4-demethylwyosine(37) in tRNA(Phe) = S-methyl-5'-thioadenosine + 7-((3S)-3-amino-3-carboxypropyl)-4-demethylwyosine(37) in tRNA(Phe).1 Publication

Pathwayi: wybutosine-tRNA(Phe) biosynthesis

This protein is involved in the pathway wybutosine-tRNA(Phe) biosynthesis, which is part of tRNA modification.1 Publication
View all proteins of this organism that are known to be involved in the pathway wybutosine-tRNA(Phe) biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei218S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei225S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei265S-adenosyl-L-methioninePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processtRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BRENDAi2.5.1.114 2681
ReactomeiR-HSA-6782861 Synthesis of wybutosine at G37 of tRNA(Phe)
UniPathwayi
UPA00375

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA wybutosine-synthesizing protein 2 homolog (EC:2.5.1.114)
Short name:
tRNA-yW-synthesizing protein 2
Alternative name(s):
tRNA(Phe) (4-demethylwyosine(37)-C(7)) aminocarboxypropyltransferase
Gene namesi
Name:TRMT12
Synonyms:TRM12, TYW2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 8

Organism-specific databases

EuPathDBiHostDB:ENSG00000183665.5
HGNCiHGNC:26091 TRMT12
MIMi611244 gene
neXtProtiNX_Q53H54

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi225K → A: Lack of activity. 1 Publication1
Mutagenesisi242Y → A: Does not affect activity. 1 Publication1
Mutagenesisi248F → A: Does not affect activity. 1 Publication1
Mutagenesisi265E → A: Lack of activity. 1 Publication1
Mutagenesisi293D → A: Does not affect activity. 1 Publication1

Organism-specific databases

DisGeNETi55039
OpenTargetsiENSG00000183665
PharmGKBiPA142670700

Polymorphism and mutation databases

BioMutaiTRMT12
DMDMi74726289

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002818361 – 448tRNA wybutosine-synthesizing protein 2 homologAdd BLAST448

Proteomic databases

EPDiQ53H54
MaxQBiQ53H54
PaxDbiQ53H54
PeptideAtlasiQ53H54
PRIDEiQ53H54
ProteomicsDBi62495

PTM databases

iPTMnetiQ53H54
PhosphoSitePlusiQ53H54

Expressioni

Gene expression databases

BgeeiENSG00000183665 Expressed in 185 organ(s), highest expression level in sperm
CleanExiHS_TRMT12
ExpressionAtlasiQ53H54 baseline and differential
GenevisibleiQ53H54 HS

Organism-specific databases

HPAiHPA023939

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
LNX1Q8TBB13EBI-10242598,EBI-739832

Protein-protein interaction databases

BioGridi120368, 6 interactors
IntActiQ53H54, 3 interactors
STRINGi9606.ENSP00000329858

Structurei

3D structure databases

ProteinModelPortaliQ53H54
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni293 – 294S-adenosyl-L-methionine bindingPROSITE-ProRule annotation2

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TRM5/TYW2 family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1227 Eukaryota
COG2520 LUCA
GeneTreeiENSGT00390000006078
HOGENOMiHOG000294185
HOVERGENiHBG108624
InParanoidiQ53H54
KOiK07055
OMAiHIQPVKS
OrthoDBiEOG091G06O6
PhylomeDBiQ53H54
TreeFamiTF314137

Family and domain databases

InterProiView protein in InterPro
IPR030382 MeTrfase_TRM5/TYW2
IPR029063 SAM-dependent_MTases
PfamiView protein in Pfam
PF02475 Met_10, 1 hit
SUPFAMiSSF53335 SSF53335, 2 hits
PROSITEiView protein in PROSITE
PS51684 SAM_MT_TRM5_TYW2, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q53H54-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MRENVVVSNM ERESGKPVAV VAVVTEPWFT QRYREYLQRQ KLFDTQHRVE
60 70 80 90 100
KMPDGSVALP VLGETLPEQH LQELRNRVAP GSPCMLTQLP DPVPSKRAQG
110 120 130 140 150
CSPAQKLCLE VSRWVEGRGV KWSAELEADL PRSWQRHGNL LLLSEDCFQA
160 170 180 190 200
KQWKNLGPEL WETVALALGV QRLAKRGRVS PDGTRTPAVT LLLGDHGWVE
210 220 230 240 250
HVDNGIRYKF DVTQCMFSFG NITEKLRVAS LSCAGEVLVD LYAGIGYFTL
260 270 280 290 300
PFLVHAGAAF VHACEWNPHA VVALRNNLEI NGVADRCQIH FGDNRKLKLS
310 320 330 340 350
NIADRVILGL IPSSEEGWPI ACQVLRQDAG GILHIHQNVE SFPGKNLQAL
360 370 380 390 400
GVSKVEKEHW LYPQQITTNQ WKNGATRDSR GKMLSPATKP EWQRWAESAE
410 420 430 440
TRIATLLQQV HGKPWKTQIL HIQPVKSYAP HVDHIVLDLE CCPCPSVG
Length:448
Mass (Da):50,236
Last modified:May 24, 2005 - v1
Checksum:i5E66C20AF26C4B5F
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E5RHH6E5RHH6_HUMAN
tRNA wybutosine-synthesizing protei...
TRMT12
150Annotation score:

Sequence cautioni

The sequence AAH03057 differs from that shown. Contaminating sequence. Potential poly-A sequence starting in position 371.Curated
The sequence AAH03057 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti342F → S in BAA91374 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_03129128W → R1 PublicationCorresponds to variant dbSNP:rs3812475Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK000779 mRNA Translation: BAA91374.1
AK222727 mRNA Translation: BAD96447.1
BC003057 mRNA Translation: AAH03057.2 Sequence problems.
BC011713 mRNA Translation: AAH11713.1
CCDSiCCDS6349.1
RefSeqiNP_060426.2, NM_017956.3
UniGeneiHs.9925

Genome annotation databases

EnsembliENST00000328599; ENSP00000329858; ENSG00000183665
GeneIDi55039
KEGGihsa:55039
UCSCiuc003yra.5 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK000779 mRNA Translation: BAA91374.1
AK222727 mRNA Translation: BAD96447.1
BC003057 mRNA Translation: AAH03057.2 Sequence problems.
BC011713 mRNA Translation: AAH11713.1
CCDSiCCDS6349.1
RefSeqiNP_060426.2, NM_017956.3
UniGeneiHs.9925

3D structure databases

ProteinModelPortaliQ53H54
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120368, 6 interactors
IntActiQ53H54, 3 interactors
STRINGi9606.ENSP00000329858

PTM databases

iPTMnetiQ53H54
PhosphoSitePlusiQ53H54

Polymorphism and mutation databases

BioMutaiTRMT12
DMDMi74726289

Proteomic databases

EPDiQ53H54
MaxQBiQ53H54
PaxDbiQ53H54
PeptideAtlasiQ53H54
PRIDEiQ53H54
ProteomicsDBi62495

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000328599; ENSP00000329858; ENSG00000183665
GeneIDi55039
KEGGihsa:55039
UCSCiuc003yra.5 human

Organism-specific databases

CTDi55039
DisGeNETi55039
EuPathDBiHostDB:ENSG00000183665.5
GeneCardsiTRMT12
HGNCiHGNC:26091 TRMT12
HPAiHPA023939
MIMi611244 gene
neXtProtiNX_Q53H54
OpenTargetsiENSG00000183665
PharmGKBiPA142670700
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1227 Eukaryota
COG2520 LUCA
GeneTreeiENSGT00390000006078
HOGENOMiHOG000294185
HOVERGENiHBG108624
InParanoidiQ53H54
KOiK07055
OMAiHIQPVKS
OrthoDBiEOG091G06O6
PhylomeDBiQ53H54
TreeFamiTF314137

Enzyme and pathway databases

UniPathwayi
UPA00375

BRENDAi2.5.1.114 2681
ReactomeiR-HSA-6782861 Synthesis of wybutosine at G37 of tRNA(Phe)

Miscellaneous databases

ChiTaRSiTRMT12 human
GeneWikiiTRMT12
GenomeRNAii55039
PROiPR:Q53H54
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000183665 Expressed in 185 organ(s), highest expression level in sperm
CleanExiHS_TRMT12
ExpressionAtlasiQ53H54 baseline and differential
GenevisibleiQ53H54 HS

Family and domain databases

InterProiView protein in InterPro
IPR030382 MeTrfase_TRM5/TYW2
IPR029063 SAM-dependent_MTases
PfamiView protein in Pfam
PF02475 Met_10, 1 hit
SUPFAMiSSF53335 SSF53335, 2 hits
PROSITEiView protein in PROSITE
PS51684 SAM_MT_TRM5_TYW2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTYW2_HUMAN
AccessioniPrimary (citable) accession number: Q53H54
Secondary accession number(s): Q6PKB9, Q96F21, Q9NWK6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: May 24, 2005
Last modified: September 12, 2018
This is version 101 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. SIMILARITY comments
    Index of protein domains and families
  4. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  5. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  6. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again