UniProtKB - Q53GT1 (KLH22_HUMAN)
Kelch-like protein 22
KLHL22
Functioni
Miscellaneous
: protein ubiquitination Pathwayi
This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.2 PublicationsView all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.
GO - Molecular functioni
- 14-3-3 protein binding Source: UniProtKB
GO - Biological processi
- cell division Source: UniProtKB
- cellular response to leucine Source: UniProtKB
- mitotic sister chromatid segregation Source: UniProtKB
- mitotic spindle assembly checkpoint Source: UniProtKB
- negative regulation of autophagy Source: UniProtKB
- positive regulation of cell growth Source: UniProtKB
- positive regulation of TORC1 signaling Source: UniProtKB
- post-translational protein modification Source: Reactome
- proteasome-mediated ubiquitin-dependent protein catabolic process Source: UniProtKB
- protein monoubiquitination Source: UniProtKB
Keywordsi
Biological process | Cell cycle, Cell division, Mitosis, Ubl conjugation pathway |
Enzyme and pathway databases
PathwayCommonsi | Q53GT1 |
Reactomei | R-HSA-8951664, Neddylation R-HSA-983168, Antigen processing: Ubiquitination & Proteasome degradation |
UniPathwayi | UPA00143 |
Names & Taxonomyi
Protein namesi | Recommended name: Kelch-like protein 22Curated |
Gene namesi | Name:KLHL22Imported |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:25888, KLHL22 |
MIMi | 618020, gene |
neXtProti | NX_Q53GT1 |
VEuPathDBi | HostDB:ENSG00000099910.16 |
Subcellular locationi
Lysosome
- Lysosome 1 Publication
Nucleus
- Nucleus 1 Publication
Cytoskeleton
- centrosome 1 Publication
- spindle 1 Publication
Cytoplasm and Cytosol
- cytosol 2 Publications
Note: Mainly cytoplasmic in prophase and prometaphase. Associates with the mitotic spindle as the cells reach chromosome bi-orientation. Localizes to the centrosomes shortly before cells enter anaphase After anaphase onset, predominantly associates with the polar microtubules connecting the 2 opposing centrosomes and gradually diffuses into the cytoplasm during telophase (PubMed:23455478). Localizes to the nucleus upon amino acid starvation (PubMed:29769719). Relocalizes to the cytosol and associates with lysosomes when amino acids are available (PubMed:29769719).2 Publications
Cytoskeleton
- centrosome Source: UniProtKB
- microtubule cytoskeleton Source: HPA
- mitotic spindle Source: UniProtKB
- polar microtubule Source: UniProtKB
Cytosol
- cytosol Source: UniProtKB
Extracellular region or secreted
- intercellular bridge Source: HPA
Lysosome
- lysosome Source: UniProtKB-SubCell
Nucleus
- nucleus Source: UniProtKB-SubCell
Other locations
- Cul3-RING ubiquitin ligase complex Source: UniProtKB
- cytoplasm Source: UniProtKB
- intracellular membrane-bounded organelle Source: HPA
Keywords - Cellular componenti
Cytoplasm, Cytoskeleton, Lysosome, NucleusPathology & Biotechi
Involvement in diseasei
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 18 | S → A: Loss of interaction with YWHAE. Loss of nuclear localization upon amino acid starvation. 1 Publication | 1 |
Keywords - Diseasei
Disease variantOrganism-specific databases
DisGeNETi | 84861 |
OpenTargetsi | ENSG00000099910 |
PharmGKBi | PA142671574 |
Miscellaneous databases
Pharosi | Q53GT1, Tbio |
Genetic variation databases
BioMutai | KLHL22 |
DMDMi | 109892504 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | |||
ChainiPRO_0000242155 | 2 – 634 | Kelch-like protein 22Add BLAST | 633 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineCombined sources | 1 | |
Modified residuei | 463 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 466 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 475 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 605 | PhosphothreonineCombined sources | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | Q53GT1 |
jPOSTi | Q53GT1 |
MassIVEi | Q53GT1 |
MaxQBi | Q53GT1 |
PaxDbi | Q53GT1 |
PeptideAtlasi | Q53GT1 |
PRIDEi | Q53GT1 |
ProteomicsDBi | 62490 [Q53GT1-1] |
PTM databases
iPTMneti | Q53GT1 |
PhosphoSitePlusi | Q53GT1 |
Expressioni
Gene expression databases
Bgeei | ENSG00000099910, Expressed in stomach and 210 other tissues |
ExpressionAtlasi | Q53GT1, baseline and differential |
Genevisiblei | Q53GT1, HS |
Organism-specific databases
HPAi | ENSG00000099910, Low tissue specificity |
Interactioni
Subunit structurei
Component of the BCR(KLHL22) E3 ubiquitin ligase complex, at least composed of CUL3, KLHL22 and RBX1 (PubMed:19995937).
Interacts with PLK1 (PubMed:24067371, PubMed:23455478).
Interacts with DEPDC5 (via DEP domain); the interaction depends on amino acid availability (PubMed:29769719).
Interacts with YWHAE; required for the nuclear localization of KLHL22 upon amino acid starvation (PubMed:29769719).
4 PublicationsBinary interactionsi
Hide detailsGO - Molecular functioni
- 14-3-3 protein binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 124308, 63 interactors |
IntActi | Q53GT1, 49 interactors |
MINTi | Q53GT1 |
STRINGi | 9606.ENSP00000331682 |
Miscellaneous databases
RNActi | Q53GT1, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 50 – 117 | BTBPROSITE-ProRule annotationAdd BLAST | 68 | |
Repeati | 299 – 349 | Kelch 1Add BLAST | 51 | |
Repeati | 350 – 399 | Kelch 2Add BLAST | 50 | |
Repeati | 400 – 446 | Kelch 3Add BLAST | 47 | |
Repeati | 448 – 493 | Kelch 4Add BLAST | 46 | |
Repeati | 494 – 544 | Kelch 5Add BLAST | 51 | |
Repeati | 545 – 593 | Kelch 6Add BLAST | 49 |
Keywords - Domaini
Kelch repeat, RepeatPhylogenomic databases
eggNOGi | KOG4441, Eukaryota |
GeneTreei | ENSGT00940000159598 |
HOGENOMi | CLU_004253_14_3_1 |
InParanoidi | Q53GT1 |
OMAi | HYHYSPE |
OrthoDBi | 250404at2759 |
PhylomeDBi | Q53GT1 |
TreeFami | TF328485 |
Family and domain databases
Gene3Di | 2.120.10.80, 1 hit |
InterProi | View protein in InterPro IPR011705, BACK IPR017096, BTB-kelch_protein IPR000210, BTB/POZ_dom IPR015915, Kelch-typ_b-propeller IPR006652, Kelch_1 IPR030575, KLHL22 IPR011333, SKP1/BTB/POZ_sf |
PANTHERi | PTHR45632:SF5, PTHR45632:SF5, 1 hit |
Pfami | View protein in Pfam PF07707, BACK, 1 hit PF00651, BTB, 1 hit PF01344, Kelch_1, 2 hits |
PIRSFi | PIRSF037037, Kelch-like_protein_gigaxonin, 1 hit |
SMARTi | View protein in SMART SM00875, BACK, 1 hit SM00225, BTB, 1 hit SM00612, Kelch, 6 hits |
SUPFAMi | SSF117281, SSF117281, 1 hit SSF54695, SSF54695, 1 hit |
PROSITEi | View protein in PROSITE PS50097, BTB, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 6 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAEEQEFTQL CKLPAQPSHP HCVNNTYRSA QHSQALLRGL LALRDSGILF
60 70 80 90 100
DVVLVVEGRH IEAHRILLAA SCDYFRGMFA GGLKEMEQEE VLIHGVSYNA
110 120 130 140 150
MCQILHFIYT SELELSLSNV QETLVAACQL QIPEIIHFCC DFLMSWVDEE
160 170 180 190 200
NILDVYRLAE LFDLSRLTEQ LDTYILKNFV AFSRTDKYRQ LPLEKVYSLL
210 220 230 240 250
SSNRLEVSCE TEVYEGALLY HYSLEQVQAD QISLHEPPKL LETVRFPLME
260 270 280 290 300
AEVLQRLHDK LDPSPLRDTV ASALMYHRNE SLQPSLQSPQ TELRSDFQCV
310 320 330 340 350
VGFGGIHSTP STVLSDQAKY LNPLLGEWKH FTASLAPRMS NQGIAVLNNF
360 370 380 390 400
VYLIGGDNNV QGFRAESRCW RYDPRHNRWF QIQSLQQEHA DLSVCVVGRY
410 420 430 440 450
IYAVAGRDYH NDLNAVERYD PATNSWAYVA PLKREVYAHA GATLEGKMYI
460 470 480 490 500
TCGRRGEDYL KETHCYDPGS NTWHTLADGP VRRAWHGMAT LLNKLYVIGG
510 520 530 540 550
SNNDAGYRRD VHQVACYSCT SGQWSSVCPL PAGHGEPGIA VLDNRIYVLG
560 570 580 590 600
GRSHNRGSRT GYVHIYDVEK DCWEEGPQLD NSISGLAACV LTLPRSLLLE
610 620 630
PPRGTPDRSQ ADPDFASEVM SVSDWEEFDN SSED
Computationally mapped potential isoform sequencesi
There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketC9JHG1 | C9JHG1_HUMAN | Kelch-like protein 22 | KLHL22 | 295 | Annotation score: | ||
C9JRG9 | C9JRG9_HUMAN | Kelch-like protein 22 | KLHL22 | 107 | Annotation score: | ||
C9J191 | C9J191_HUMAN | Kelch-like protein 22 | KLHL22 | 225 | Annotation score: | ||
C9J2T1 | C9J2T1_HUMAN | Kelch-like protein 22 | KLHL22 | 235 | Annotation score: | ||
C9J5F8 | C9J5F8_HUMAN | Kelch-like protein 22 | KLHL22 | 251 | Annotation score: | ||
C9JLE9 | C9JLE9_HUMAN | Kelch-like protein 22 | KLHL22 | 158 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 352 | Y → H in BAD96570 (Ref. 3) Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_079854 | 20 | P → Q Found in a patient with isolated coloboma; unknown pathological significance. 1 Publication | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_057028 | 1 – 143 | Missing in isoform 2. 1 PublicationAdd BLAST | 143 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CR456352 mRNA Translation: CAG30238.1 AK027266 mRNA Translation: BAB55007.1 Sequence problems. AK290669 mRNA Translation: BAF83358.1 AK294682 mRNA Translation: BAH11847.1 AK222850 mRNA Translation: BAD96570.1 AC007731 Genomic DNA No translation available. CH471176 Genomic DNA Translation: EAX02961.1 CH471176 Genomic DNA Translation: EAX02962.1 CH471176 Genomic DNA Translation: EAX02963.1 BC015923 mRNA Translation: AAH15923.1 |
CCDSi | CCDS13780.1 [Q53GT1-1] |
RefSeqi | NP_116164.2, NM_032775.3 [Q53GT1-1] XP_016884510.1, XM_017029021.1 [Q53GT1-1] |
Genome annotation databases
Ensembli | ENST00000328879; ENSP00000331682; ENSG00000099910 [Q53GT1-1] |
GeneIDi | 84861 |
KEGGi | hsa:84861 |
UCSCi | uc002zsl.2, human [Q53GT1-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CR456352 mRNA Translation: CAG30238.1 AK027266 mRNA Translation: BAB55007.1 Sequence problems. AK290669 mRNA Translation: BAF83358.1 AK294682 mRNA Translation: BAH11847.1 AK222850 mRNA Translation: BAD96570.1 AC007731 Genomic DNA No translation available. CH471176 Genomic DNA Translation: EAX02961.1 CH471176 Genomic DNA Translation: EAX02962.1 CH471176 Genomic DNA Translation: EAX02963.1 BC015923 mRNA Translation: AAH15923.1 |
CCDSi | CCDS13780.1 [Q53GT1-1] |
RefSeqi | NP_116164.2, NM_032775.3 [Q53GT1-1] XP_016884510.1, XM_017029021.1 [Q53GT1-1] |
3D structure databases
SMRi | Q53GT1 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 124308, 63 interactors |
IntActi | Q53GT1, 49 interactors |
MINTi | Q53GT1 |
STRINGi | 9606.ENSP00000331682 |
PTM databases
iPTMneti | Q53GT1 |
PhosphoSitePlusi | Q53GT1 |
Genetic variation databases
BioMutai | KLHL22 |
DMDMi | 109892504 |
Proteomic databases
EPDi | Q53GT1 |
jPOSTi | Q53GT1 |
MassIVEi | Q53GT1 |
MaxQBi | Q53GT1 |
PaxDbi | Q53GT1 |
PeptideAtlasi | Q53GT1 |
PRIDEi | Q53GT1 |
ProteomicsDBi | 62490 [Q53GT1-1] |
Protocols and materials databases
Antibodypediai | 34893, 180 antibodies |
DNASUi | 84861 |
Genome annotation databases
Ensembli | ENST00000328879; ENSP00000331682; ENSG00000099910 [Q53GT1-1] |
GeneIDi | 84861 |
KEGGi | hsa:84861 |
UCSCi | uc002zsl.2, human [Q53GT1-1] |
Organism-specific databases
CTDi | 84861 |
DisGeNETi | 84861 |
GeneCardsi | KLHL22 |
HGNCi | HGNC:25888, KLHL22 |
HPAi | ENSG00000099910, Low tissue specificity |
MIMi | 618020, gene |
neXtProti | NX_Q53GT1 |
OpenTargetsi | ENSG00000099910 |
PharmGKBi | PA142671574 |
VEuPathDBi | HostDB:ENSG00000099910.16 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG4441, Eukaryota |
GeneTreei | ENSGT00940000159598 |
HOGENOMi | CLU_004253_14_3_1 |
InParanoidi | Q53GT1 |
OMAi | HYHYSPE |
OrthoDBi | 250404at2759 |
PhylomeDBi | Q53GT1 |
TreeFami | TF328485 |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
PathwayCommonsi | Q53GT1 |
Reactomei | R-HSA-8951664, Neddylation R-HSA-983168, Antigen processing: Ubiquitination & Proteasome degradation |
Miscellaneous databases
BioGRID-ORCSi | 84861, 17 hits in 998 CRISPR screens |
ChiTaRSi | KLHL22, human |
GenomeRNAii | 84861 |
Pharosi | Q53GT1, Tbio |
PROi | PR:Q53GT1 |
RNActi | Q53GT1, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000099910, Expressed in stomach and 210 other tissues |
ExpressionAtlasi | Q53GT1, baseline and differential |
Genevisiblei | Q53GT1, HS |
Family and domain databases
Gene3Di | 2.120.10.80, 1 hit |
InterProi | View protein in InterPro IPR011705, BACK IPR017096, BTB-kelch_protein IPR000210, BTB/POZ_dom IPR015915, Kelch-typ_b-propeller IPR006652, Kelch_1 IPR030575, KLHL22 IPR011333, SKP1/BTB/POZ_sf |
PANTHERi | PTHR45632:SF5, PTHR45632:SF5, 1 hit |
Pfami | View protein in Pfam PF07707, BACK, 1 hit PF00651, BTB, 1 hit PF01344, Kelch_1, 2 hits |
PIRSFi | PIRSF037037, Kelch-like_protein_gigaxonin, 1 hit |
SMARTi | View protein in SMART SM00875, BACK, 1 hit SM00225, BTB, 1 hit SM00612, Kelch, 6 hits |
SUPFAMi | SSF117281, SSF117281, 1 hit SSF54695, SSF54695, 1 hit |
PROSITEi | View protein in PROSITE PS50097, BTB, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | KLH22_HUMAN | |
Accessioni | Q53GT1Primary (citable) accession number: Q53GT1 Secondary accession number(s): A8K3Q4 Q96KC6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 27, 2006 |
Last sequence update: | June 27, 2006 | |
Last modified: | April 7, 2021 | |
This is version 138 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 22
Human chromosome 22: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways