UniProtKB - Q53GL0 (PKHO1_HUMAN)
Pleckstrin homology domain-containing family O member 1
PLEKHO1
Functioni
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 155 | Capping protein | 1 | |
Binding sitei | 157 | Capping protein | 1 |
GO - Biological processi
- lamellipodium morphogenesis Source: Ensembl
- myoblast fusion Source: Ensembl
- myoblast migration Source: Ensembl
- regulation of cell shape Source: Ensembl
Enzyme and pathway databases
PathwayCommonsi | Q53GL0 |
Names & Taxonomyi
Protein namesi | Recommended name: Pleckstrin homology domain-containing family O member 1Short name: PH domain-containing family O member 1 Alternative name(s): C-Jun-binding protein Short name: JBP Casein kinase 2-interacting protein 1 Short name: CK2-interacting protein 1 Short name: CKIP-1 Osteoclast maturation-associated gene 120 protein |
Gene namesi | Name:PLEKHO1 Synonyms:CKIP1, OC120 ORF Names:HQ0024c |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:24310, PLEKHO1 |
MIMi | 608335, gene |
neXtProti | NX_Q53GL0 |
VEuPathDBi | HostDB:ENSG00000023902.13 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Plasma membrane
- Cell membrane 5 Publications; Peripheral membrane protein 1 Publication
Nucleus
- Nucleus 3 Publications
Note: Predominantly localized to the plasma membrane through the binding to phosphatidylinositol 3-phosphate (PubMed:14729969). In C2C12 cells, with the absence of growth factor, it is found in the nucleus (PubMed:14729969). It rapidly translocates to the plasma membrane after insulin stimulation (PubMed:14729969). In response to TNF, it translocates from the plasma membrane to the cytoplasm and then to the nucleus accompanied by cleavage by caspase-3 (PubMed:15706351). However, the subcellular location is highly dependent of the cell type, and this explains why it is found exclusively at the plasma membrane, in some type of cells (Probable).Curated2 Publications
Nucleus
- nucleus Source: UniProtKB-SubCell
Plasma Membrane
- muscle cell projection membrane Source: Ensembl
- ruffle membrane Source: Ensembl
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cell membrane, Cytoplasm, Membrane, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 42 | K → C: No effect on subcellular localization. No effect on subcellular localization; when associated with C-44. Disruption of membrane localization, loss of phospholipid binding and impaired interaction with CK2; when associated with W-123. Disruption of membrane localization, loss of phospholipid binding and impaired interaction with CK2; when associated with C-44 and W-123. 1 Publication | 1 | |
Mutagenesisi | 44 | R → C: No effect on subcellular localization. No effect on subcellular localization; when associated with C-42. Disruption of membrane localization, loss of phospholipid binding and impaired interaction with CK2; when associated with W-123. Disruption of membrane localization, loss of phospholipid binding and impaired interaction with CK2; when associated with C-42 and W-123. 1 Publication | 1 | |
Mutagenesisi | 123 | W → A: Disruption of membrane localization and impaired interaction with CK2. Loss of phospholipid binding; when associated with C-42. Loss of phospholipid binding; when associated with C-44. Disruption of membrane localization, loss of phospholipid binding and impaired interaction with CK2; when associated with C-42 and C-44. 1 Publication | 1 | |
Mutagenesisi | 133 | R → A: No effect on binding to capping proteins and loss of phospholipid binding; when associated with A-135 and A-137. | 1 | |
Mutagenesisi | 133 | R → E: No effect on binding to capping proteins; when associated with E-135. | 1 | |
Mutagenesisi | 135 | K → A: No effect on binding to capping proteins; when associated with A-133 and A-137. | 1 | |
Mutagenesisi | 135 | K → E: No effect on binding to capping proteins; when associated with E-133. | 1 | |
Mutagenesisi | 137 | R → A: No effect on binding to capping proteins; when associated with A-133 and A-135. | 1 | |
Mutagenesisi | 155 | R → A: No change in cell morphology and actin cytoskeleton. Great loss of binding to capping proteins; when associated with A-157. Great loss of binding to capping proteins; when associated with A-157 and A-159. 1 Publication | 1 | |
Mutagenesisi | 155 | R → E: No change in cell morphology and actin cytoskeleton. Great loss of binding to capping proteins and no change in cell morphology and actin cytoskeleton; when associated with E-157. 1 Publication | 1 | |
Mutagenesisi | 157 | R → A: No change in cell morphology and actin cytoskeleton. Great loss of binding to capping proteins; when associated with A-155. Great loss of binding to capping proteins; when associated with A-155 and A-159. 1 Publication | 1 | |
Mutagenesisi | 157 | R → E: No change in cell morphology and actin cytoskeleton. Great loss of binding to capping proteins and no change in cell morphology and actin cytoskeleton; when associated with E-155. 1 Publication | 1 | |
Mutagenesisi | 159 | K → A: Great loss of binding to capping proteins; when associated with A-155 and A-157. | 1 |
Keywords - Diseasei
Tumor suppressorOrganism-specific databases
DisGeNETi | 51177 |
OpenTargetsi | ENSG00000023902 |
PharmGKBi | PA142671167 |
Miscellaneous databases
Pharosi | Q53GL0, Tbio |
Genetic variation databases
BioMutai | PLEKHO1 |
DMDMi | 160419242 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000310423 | 1 – 409 | Pleckstrin homology domain-containing family O member 1Add BLAST | 409 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 227 | PhosphoserineBy similarity | 1 | |
Modified residuei | 271 | PhosphoserineBy similarity | 1 | |
Modified residuei | 342 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 310 – 311 | Cleavage; by caspase-3Sequence analysis | 2 | |
Sitei | 345 – 346 | Cleavage; by caspase-3Sequence analysis | 2 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q53GL0 |
jPOSTi | Q53GL0 |
MassIVEi | Q53GL0 |
MaxQBi | Q53GL0 |
PaxDbi | Q53GL0 |
PeptideAtlasi | Q53GL0 |
PRIDEi | Q53GL0 |
ProteomicsDBi | 62483 [Q53GL0-1] 62484 [Q53GL0-2] |
PTM databases
iPTMneti | Q53GL0 |
PhosphoSitePlusi | Q53GL0 |
Expressioni
Tissue specificityi
Inductioni
Gene expression databases
Bgeei | ENSG00000023902, Expressed in lower esophagus muscularis layer and 214 other tissues |
ExpressionAtlasi | Q53GL0, baseline and differential |
Genevisiblei | Q53GL0, HS |
Organism-specific databases
HPAi | ENSG00000023902, Tissue enhanced (smooth) |
Interactioni
Subunit structurei
Heterodimer or homodimer.
Interacts with CK2 and actin capping subunits (capping protein CP-alpha and CP-beta). CKIP1 and CK2 together inhibit the activity of actin capping protein at the barbed ends of actin filaments.
Interacts with ATM, IFP35, JUN, JUND, NMI and PI3K.
Interacts with AKT1, AKT2 and AKT3 (each isozyme of PKB), PtdIns(3,5)P2, PtdIns(4,5)P2 and PtdIns(3,4,5)P2.
10 PublicationsBinary interactionsi
Hide detailsQ53GL0
Protein-protein interaction databases
BioGRIDi | 119355, 86 interactors |
DIPi | DIP-46903N |
IntActi | Q53GL0, 78 interactors |
MINTi | Q53GL0 |
STRINGi | 9606.ENSP00000358120 |
Miscellaneous databases
RNActi | Q53GL0, protein |
Structurei
Secondary structure
3D structure databases
SMRi | Q53GL0 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q53GL0 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 21 – 132 | PHPROSITE-ProRule annotationAdd BLAST | 112 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 133 – 193 | Interaction with capping proteins (CPs)Add BLAST | 61 | |
Regioni | 136 – 308 | Interaction with ATM, CKIP, IFP35 and NMI2 PublicationsAdd BLAST | 173 | |
Regioni | 308 – 409 | Negative regulator of AP-1 activityAdd BLAST | 102 |
Phylogenomic databases
eggNOGi | ENOG502QSPU, Eukaryota |
GeneTreei | ENSGT00530000063760 |
HOGENOMi | CLU_052292_0_0_1 |
InParanoidi | Q53GL0 |
OMAi | KPDRGAT |
OrthoDBi | 929437at2759 |
PhylomeDBi | Q53GL0 |
TreeFami | TF333115 |
Family and domain databases
Gene3Di | 2.30.29.30, 1 hit |
InterProi | View protein in InterPro IPR028452, CKIP-1 IPR011993, PH-like_dom_sf IPR001849, PH_domain IPR043448, PKHO1/2 |
PANTHERi | PTHR15871, PTHR15871, 1 hit PTHR15871:SF1, PTHR15871:SF1, 1 hit |
Pfami | View protein in Pfam PF00169, PH, 1 hit |
SMARTi | View protein in SMART SM00233, PH, 1 hit |
PROSITEi | View protein in PROSITE PS50003, PH_DOMAIN, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MMKKNNSAKR GPQDGNQQPA PPEKVGWVRK FCGKGIFREI WKNRYVVLKG
60 70 80 90 100
DQLYISEKEV KDEKNIQEVF DLSDYEKCEE LRKSKSRSKK NHSKFTLAHS
110 120 130 140 150
KQPGNTAPNL IFLAVSPEEK ESWINALNSA ITRAKNRILD EVTVEEDSYL
160 170 180 190 200
AHPTRDRAKI QHSRRPPTRG HLMAVASTST SDGMLTLDLI QEEDPSPEEP
210 220 230 240 250
TSCAESFRVD LDKSVAQLAG SRRRADSDRI QPSADRASSL SRPWEKTDKG
260 270 280 290 300
ATYTPQAPKK LTPTEKGRCA SLEEILSQRD AASARTLQLR AEEPPTPALP
310 320 330 340 350
NPGQLSRIQD LVARKLEETQ ELLAEVQGLG DGKRKAKDPP RSPPDSESEQ
360 370 380 390 400
LLLETERLLG EASSNWSQAK RVLQEVRELR DLYRQMDLQT PDSHLRQTTP
HSQYRKSLM
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketQ5T4P9 | Q5T4P9_HUMAN | Pleckstrin homology domain containi... | PLEKHO1 hCG_39382, RP11-458I7.3-002 | 226 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 1 – 10 | MMKKNNSAKR → VRRCCRGWRSPVFTNPHVSP LSTAPAHWAPGRPAAGSLGL RVSPEPPPERGSLPPGERSP AASKPPSSPS in AAH23533 (PubMed:15489334).Curated | 10 | |
Sequence conflicti | 285 | R → H in BAD96641 (Ref. 5) Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_037034 | 21 | P → A. Corresponds to variant dbSNP:rs2306235Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_029282 | 142 – 175 | Missing in isoform 2. CuratedAdd BLAST | 34 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF168676 mRNA Translation: AAF89644.1 AF192912 mRNA Translation: AAQ13826.1 Different initiation. AF291105 mRNA Translation: AAK28027.1 AF217956 mRNA Translation: AAK71509.1 AK222921 mRNA Translation: BAD96641.1 AL358073 Genomic DNA Translation: CAI14264.1 CH471121 Genomic DNA Translation: EAW53580.1 BC010149 mRNA Translation: AAH10149.1 BC023533 mRNA Translation: AAH23533.2 AF073836 mRNA Translation: AAF13461.1 Different initiation. |
CCDSi | CCDS945.1 [Q53GL0-1] |
RefSeqi | NP_001291651.1, NM_001304722.1 NP_001291652.1, NM_001304723.1 NP_001291653.1, NM_001304724.1 NP_057358.2, NM_016274.5 [Q53GL0-1] XP_016856909.1, XM_017001420.1 |
Genome annotation databases
Ensembli | ENST00000369124; ENSP00000358120; ENSG00000023902 [Q53GL0-1] |
GeneIDi | 51177 |
KEGGi | hsa:51177 |
UCSCi | uc001ett.4, human [Q53GL0-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF168676 mRNA Translation: AAF89644.1 AF192912 mRNA Translation: AAQ13826.1 Different initiation. AF291105 mRNA Translation: AAK28027.1 AF217956 mRNA Translation: AAK71509.1 AK222921 mRNA Translation: BAD96641.1 AL358073 Genomic DNA Translation: CAI14264.1 CH471121 Genomic DNA Translation: EAW53580.1 BC010149 mRNA Translation: AAH10149.1 BC023533 mRNA Translation: AAH23533.2 AF073836 mRNA Translation: AAF13461.1 Different initiation. |
CCDSi | CCDS945.1 [Q53GL0-1] |
RefSeqi | NP_001291651.1, NM_001304722.1 NP_001291652.1, NM_001304723.1 NP_001291653.1, NM_001304724.1 NP_057358.2, NM_016274.5 [Q53GL0-1] XP_016856909.1, XM_017001420.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3AA1 | X-ray | 1.90 | C | 148-170 | [»] | |
SMRi | Q53GL0 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 119355, 86 interactors |
DIPi | DIP-46903N |
IntActi | Q53GL0, 78 interactors |
MINTi | Q53GL0 |
STRINGi | 9606.ENSP00000358120 |
PTM databases
iPTMneti | Q53GL0 |
PhosphoSitePlusi | Q53GL0 |
Genetic variation databases
BioMutai | PLEKHO1 |
DMDMi | 160419242 |
Proteomic databases
EPDi | Q53GL0 |
jPOSTi | Q53GL0 |
MassIVEi | Q53GL0 |
MaxQBi | Q53GL0 |
PaxDbi | Q53GL0 |
PeptideAtlasi | Q53GL0 |
PRIDEi | Q53GL0 |
ProteomicsDBi | 62483 [Q53GL0-1] 62484 [Q53GL0-2] |
Protocols and materials databases
Antibodypediai | 35196, 183 antibodies |
DNASUi | 51177 |
Genome annotation databases
Ensembli | ENST00000369124; ENSP00000358120; ENSG00000023902 [Q53GL0-1] |
GeneIDi | 51177 |
KEGGi | hsa:51177 |
UCSCi | uc001ett.4, human [Q53GL0-1] |
Organism-specific databases
CTDi | 51177 |
DisGeNETi | 51177 |
GeneCardsi | PLEKHO1 |
HGNCi | HGNC:24310, PLEKHO1 |
HPAi | ENSG00000023902, Tissue enhanced (smooth) |
MIMi | 608335, gene |
neXtProti | NX_Q53GL0 |
OpenTargetsi | ENSG00000023902 |
PharmGKBi | PA142671167 |
VEuPathDBi | HostDB:ENSG00000023902.13 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502QSPU, Eukaryota |
GeneTreei | ENSGT00530000063760 |
HOGENOMi | CLU_052292_0_0_1 |
InParanoidi | Q53GL0 |
OMAi | KPDRGAT |
OrthoDBi | 929437at2759 |
PhylomeDBi | Q53GL0 |
TreeFami | TF333115 |
Enzyme and pathway databases
PathwayCommonsi | Q53GL0 |
Miscellaneous databases
BioGRID-ORCSi | 51177, 8 hits in 1005 CRISPR screens |
ChiTaRSi | PLEKHO1, human |
EvolutionaryTracei | Q53GL0 |
GeneWikii | PLEKHO1 |
GenomeRNAii | 51177 |
Pharosi | Q53GL0, Tbio |
PROi | PR:Q53GL0 |
RNActi | Q53GL0, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000023902, Expressed in lower esophagus muscularis layer and 214 other tissues |
ExpressionAtlasi | Q53GL0, baseline and differential |
Genevisiblei | Q53GL0, HS |
Family and domain databases
Gene3Di | 2.30.29.30, 1 hit |
InterProi | View protein in InterPro IPR028452, CKIP-1 IPR011993, PH-like_dom_sf IPR001849, PH_domain IPR043448, PKHO1/2 |
PANTHERi | PTHR15871, PTHR15871, 1 hit PTHR15871:SF1, PTHR15871:SF1, 1 hit |
Pfami | View protein in Pfam PF00169, PH, 1 hit |
SMARTi | View protein in SMART SM00233, PH, 1 hit |
PROSITEi | View protein in PROSITE PS50003, PH_DOMAIN, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PKHO1_HUMAN | |
Accessioni | Q53GL0Primary (citable) accession number: Q53GL0 Secondary accession number(s): Q336K5 Q9UL48 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 13, 2007 |
Last sequence update: | November 13, 2007 | |
Last modified: | April 7, 2021 | |
This is version 126 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 1
Human chromosome 1: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references