Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Pleckstrin homology domain-containing family O member 1

Gene

PLEKHO1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays a role in the regulation of the actin cytoskeleton through its interactions with actin capping protein (CP). May function to target CK2 to the plasma membrane thereby serving as an adapter to facilitate the phosphorylation of CP by protein kinase 2 (CK2). Appears to target ATM to the plasma membrane. Appears to also inhibit tumor cell growth by inhibiting AKT-mediated cell-survival. Also implicated in PI3K-regulated muscle differentiation, the regulation of AP-1 activity (plasma membrane bound AP-1 regulator that translocates to the nucleus) and the promotion of apoptosis induced by tumor necrosis factor TNF. When bound to PKB, it inhibits it probably by decreasing PKB level of phosphorylation.7 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei155Capping protein1
Binding sitei157Capping protein1

GO - Biological processi

Names & Taxonomyi

Protein namesi
Recommended name:
Pleckstrin homology domain-containing family O member 1
Short name:
PH domain-containing family O member 1
Alternative name(s):
C-Jun-binding protein
Short name:
JBP
Casein kinase 2-interacting protein 1
Short name:
CK2-interacting protein 1
Short name:
CKIP-1
Osteoclast maturation-associated gene 120 protein
Gene namesi
Name:PLEKHO1
Synonyms:CKIP1, OC120
ORF Names:HQ0024c
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiHostDB:ENSG00000023902.13
HGNCiHGNC:24310 PLEKHO1
MIMi608335 gene
neXtProtiNX_Q53GL0

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi42K → C: No effect on subcellular localization. No effect on subcellular localization; when associated with C-44. Disruption of membrane localization, loss of phospholipid binding and impaired interaction with CK2; when associated with W-123. Disruption of membrane localization, loss of phospholipid binding and impaired interaction with CK2; when associated with C-44 and W-123. 1 Publication1
Mutagenesisi44R → C: No effect on subcellular localization. No effect on subcellular localization; when associated with C-42. Disruption of membrane localization, loss of phospholipid binding and impaired interaction with CK2; when associated with W-123. Disruption of membrane localization, loss of phospholipid binding and impaired interaction with CK2; when associated with C-42 and W-123. 1 Publication1
Mutagenesisi123W → A: Disruption of membrane localization and impaired interaction with CK2. Loss of phospholipid binding; when associated with C-42. Loss of phospholipid binding; when associated with C-44. Disruption of membrane localization, loss of phospholipid binding and impaired interaction with CK2; when associated with C-42 and C-44. 1 Publication1
Mutagenesisi133R → A: No effect on binding to capping proteins and loss of phospholipid binding; when associated with A-135 and A-137. 1
Mutagenesisi133R → E: No effect on binding to capping proteins; when associated with E-135. 1
Mutagenesisi135K → A: No effect on binding to capping proteins; when associated with A-133 and A-137. 1
Mutagenesisi135K → E: No effect on binding to capping proteins; when associated with E-133. 1
Mutagenesisi137R → A: No effect on binding to capping proteins; when associated with A-133 and A-135. 1
Mutagenesisi155R → A: No change in cell morphology and actin cytoskeleton. Great loss of binding to capping proteins; when associated with A-157. Great loss of binding to capping proteins; when associated with A-157 and A-159. 1 Publication1
Mutagenesisi155R → E: No change in cell morphology and actin cytoskeleton. Great loss of binding to capping proteins and no change in cell morphology and actin cytoskeleton; when associated with E-157. 1 Publication1
Mutagenesisi157R → A: No change in cell morphology and actin cytoskeleton. Great loss of binding to capping proteins; when associated with A-155. Great loss of binding to capping proteins; when associated with A-155 and A-159. 1 Publication1
Mutagenesisi157R → E: No change in cell morphology and actin cytoskeleton. Great loss of binding to capping proteins and no change in cell morphology and actin cytoskeleton; when associated with E-155. 1 Publication1
Mutagenesisi159K → A: Great loss of binding to capping proteins; when associated with A-155 and A-157. 1

Keywords - Diseasei

Tumor suppressor

Organism-specific databases

DisGeNETi51177
OpenTargetsiENSG00000023902
PharmGKBiPA142671167

Polymorphism and mutation databases

BioMutaiPLEKHO1
DMDMi160419242

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003104231 – 409Pleckstrin homology domain-containing family O member 1Add BLAST409

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei227PhosphoserineBy similarity1
Modified residuei271PhosphoserineBy similarity1
Modified residuei342PhosphoserineBy similarity1

Post-translational modificationi

C-terminal fragments could be released during apoptosis via caspase-3-dependent cleavage.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei310 – 311Cleavage; by caspase-3Sequence analysis2
Sitei345 – 346Cleavage; by caspase-3Sequence analysis2

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ53GL0
MaxQBiQ53GL0
PaxDbiQ53GL0
PeptideAtlasiQ53GL0
PRIDEiQ53GL0
ProteomicsDBi62483
62484 [Q53GL0-2]

PTM databases

iPTMnetiQ53GL0
PhosphoSitePlusiQ53GL0

Expressioni

Tissue specificityi

Abundantly expressed in skeletal muscle and heart, moderately in kidney, liver, brain and placenta and sparingly in the pancreas and lung. Easily detectable in cell lines such as MOLT-4, HEK293 and Jurkat.1 Publication

Inductioni

Up-regulated by IFNG/IFN-gamma and IL2/interleukin-2 or in C2C12 cells.2 Publications

Gene expression databases

BgeeiENSG00000023902 Expressed in 201 organ(s), highest expression level in lower esophagus muscularis layer
CleanExiHS_PLEKHO1
ExpressionAtlasiQ53GL0 baseline and differential
GenevisibleiQ53GL0 HS

Organism-specific databases

HPAiHPA061018

Interactioni

Subunit structurei

Heterodimer or homodimer. Interacts with CK2 and actin capping subunits (capping protein CP-alpha and CP-beta). CKIP1 and CK2 together inhibit the activity of actin capping protein at the barbed ends of actin filaments. Interacts with ATM, IFP35, JUN, JUND, NMI and PI3K. Interacts with AKT1, AKT2 and AKT3 (each isozyme of PKB), PtdIns(3,5)P2, PtdIns(4,5)P2 and PtdIns(3,4,5)P2.10 Publications

Binary interactionsi

Protein-protein interaction databases

BioGridi119355, 62 interactors
DIPiDIP-46903N
IntActiQ53GL0, 42 interactors
MINTiQ53GL0
STRINGi9606.ENSP00000358120

Structurei

Secondary structure

1409
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ53GL0
SMRiQ53GL0
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ53GL0

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini21 – 132PHPROSITE-ProRule annotationAdd BLAST112

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni133 – 193Interaction with capping proteins (CPs)Add BLAST61
Regioni136 – 308Interaction with ATM, CKIP, IFP35 and NMI2 PublicationsAdd BLAST173
Regioni308 – 409Negative regulator of AP-1 activityAdd BLAST102

Phylogenomic databases

eggNOGiENOG410IEJU Eukaryota
ENOG4110ACW LUCA
GeneTreeiENSGT00530000063760
HOGENOMiHOG000115563
HOVERGENiHBG108259
InParanoidiQ53GL0
OMAiKRGPQDG
OrthoDBiEOG091G0CAS
PhylomeDBiQ53GL0
TreeFamiTF333115

Family and domain databases

Gene3Di2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR028452 CKIP-1
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
PANTHERiPTHR15871:SF1 PTHR15871:SF1, 1 hit
PfamiView protein in Pfam
PF00169 PH, 1 hit
SMARTiView protein in SMART
SM00233 PH, 1 hit
PROSITEiView protein in PROSITE
PS50003 PH_DOMAIN, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: Q53GL0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MMKKNNSAKR GPQDGNQQPA PPEKVGWVRK FCGKGIFREI WKNRYVVLKG
60 70 80 90 100
DQLYISEKEV KDEKNIQEVF DLSDYEKCEE LRKSKSRSKK NHSKFTLAHS
110 120 130 140 150
KQPGNTAPNL IFLAVSPEEK ESWINALNSA ITRAKNRILD EVTVEEDSYL
160 170 180 190 200
AHPTRDRAKI QHSRRPPTRG HLMAVASTST SDGMLTLDLI QEEDPSPEEP
210 220 230 240 250
TSCAESFRVD LDKSVAQLAG SRRRADSDRI QPSADRASSL SRPWEKTDKG
260 270 280 290 300
ATYTPQAPKK LTPTEKGRCA SLEEILSQRD AASARTLQLR AEEPPTPALP
310 320 330 340 350
NPGQLSRIQD LVARKLEETQ ELLAEVQGLG DGKRKAKDPP RSPPDSESEQ
360 370 380 390 400
LLLETERLLG EASSNWSQAK RVLQEVRELR DLYRQMDLQT PDSHLRQTTP

HSQYRKSLM
Length:409
Mass (Da):46,237
Last modified:November 13, 2007 - v2
Checksum:i5077D6B559EB9F78
GO
Isoform 2 (identifier: Q53GL0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     142-175: Missing.

Note: No experimental confirmation available.
Show »
Length:375
Mass (Da):42,330
Checksum:i65CC4D44156031EA
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q5T4P9Q5T4P9_HUMAN
Pleckstrin homology domain containi...
PLEKHO1 hCG_39382, RP11-458I7.3-002
226Annotation score:

Sequence cautioni

The sequence AAF13461 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAQ13826 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1 – 10MMKKNNSAKR → VRRCCRGWRSPVFTNPHVSP LSTAPAHWAPGRPAAGSLGL RVSPEPPPERGSLPPGERSP AASKPPSSPS in AAH23533 (PubMed:15489334).Curated10
Sequence conflicti285R → H in BAD96641 (Ref. 5) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_03703421P → A. Corresponds to variant dbSNP:rs2306235Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_029282142 – 175Missing in isoform 2. CuratedAdd BLAST34

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF168676 mRNA Translation: AAF89644.1
AF192912 mRNA Translation: AAQ13826.1 Different initiation.
AF291105 mRNA Translation: AAK28027.1
AF217956 mRNA Translation: AAK71509.1
AK222921 mRNA Translation: BAD96641.1
AL358073 Genomic DNA Translation: CAI14264.1
CH471121 Genomic DNA Translation: EAW53580.1
BC010149 mRNA Translation: AAH10149.1
BC023533 mRNA Translation: AAH23533.2
AF073836 mRNA Translation: AAF13461.1 Different initiation.
CCDSiCCDS945.1 [Q53GL0-1]
RefSeqiNP_001291651.1, NM_001304722.1
NP_001291652.1, NM_001304723.1
NP_001291653.1, NM_001304724.1
NP_057358.2, NM_016274.5 [Q53GL0-1]
XP_016856909.1, XM_017001420.1
UniGeneiHs.438824

Genome annotation databases

EnsembliENST00000369124; ENSP00000358120; ENSG00000023902 [Q53GL0-1]
GeneIDi51177
KEGGihsa:51177
UCSCiuc001ett.4 human [Q53GL0-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF168676 mRNA Translation: AAF89644.1
AF192912 mRNA Translation: AAQ13826.1 Different initiation.
AF291105 mRNA Translation: AAK28027.1
AF217956 mRNA Translation: AAK71509.1
AK222921 mRNA Translation: BAD96641.1
AL358073 Genomic DNA Translation: CAI14264.1
CH471121 Genomic DNA Translation: EAW53580.1
BC010149 mRNA Translation: AAH10149.1
BC023533 mRNA Translation: AAH23533.2
AF073836 mRNA Translation: AAF13461.1 Different initiation.
CCDSiCCDS945.1 [Q53GL0-1]
RefSeqiNP_001291651.1, NM_001304722.1
NP_001291652.1, NM_001304723.1
NP_001291653.1, NM_001304724.1
NP_057358.2, NM_016274.5 [Q53GL0-1]
XP_016856909.1, XM_017001420.1
UniGeneiHs.438824

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3AA1X-ray1.90C148-170[»]
ProteinModelPortaliQ53GL0
SMRiQ53GL0
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi119355, 62 interactors
DIPiDIP-46903N
IntActiQ53GL0, 42 interactors
MINTiQ53GL0
STRINGi9606.ENSP00000358120

PTM databases

iPTMnetiQ53GL0
PhosphoSitePlusiQ53GL0

Polymorphism and mutation databases

BioMutaiPLEKHO1
DMDMi160419242

Proteomic databases

EPDiQ53GL0
MaxQBiQ53GL0
PaxDbiQ53GL0
PeptideAtlasiQ53GL0
PRIDEiQ53GL0
ProteomicsDBi62483
62484 [Q53GL0-2]

Protocols and materials databases

DNASUi51177
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000369124; ENSP00000358120; ENSG00000023902 [Q53GL0-1]
GeneIDi51177
KEGGihsa:51177
UCSCiuc001ett.4 human [Q53GL0-1]

Organism-specific databases

CTDi51177
DisGeNETi51177
EuPathDBiHostDB:ENSG00000023902.13
GeneCardsiPLEKHO1
HGNCiHGNC:24310 PLEKHO1
HPAiHPA061018
MIMi608335 gene
neXtProtiNX_Q53GL0
OpenTargetsiENSG00000023902
PharmGKBiPA142671167
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IEJU Eukaryota
ENOG4110ACW LUCA
GeneTreeiENSGT00530000063760
HOGENOMiHOG000115563
HOVERGENiHBG108259
InParanoidiQ53GL0
OMAiKRGPQDG
OrthoDBiEOG091G0CAS
PhylomeDBiQ53GL0
TreeFamiTF333115

Miscellaneous databases

ChiTaRSiPLEKHO1 human
EvolutionaryTraceiQ53GL0
GeneWikiiPLEKHO1
GenomeRNAii51177
PROiPR:Q53GL0
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000023902 Expressed in 201 organ(s), highest expression level in lower esophagus muscularis layer
CleanExiHS_PLEKHO1
ExpressionAtlasiQ53GL0 baseline and differential
GenevisibleiQ53GL0 HS

Family and domain databases

Gene3Di2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR028452 CKIP-1
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
PANTHERiPTHR15871:SF1 PTHR15871:SF1, 1 hit
PfamiView protein in Pfam
PF00169 PH, 1 hit
SMARTiView protein in SMART
SM00233 PH, 1 hit
PROSITEiView protein in PROSITE
PS50003 PH_DOMAIN, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPKHO1_HUMAN
AccessioniPrimary (citable) accession number: Q53GL0
Secondary accession number(s): Q336K5
, Q8IZ51, Q9NRV3, Q9UL48
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: November 13, 2007
Last modified: November 7, 2018
This is version 112 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again