UniProtKB - Q53F39 (MPPE1_HUMAN)
Protein
Metallophosphoesterase 1
Gene
MPPE1
Organism
Homo sapiens (Human)
Status
Functioni
Metallophosphoesterase required for transport of GPI-anchor proteins from the endoplasmic reticulum to the Golgi. Acts in lipid remodeling steps of GPI-anchor maturation by mediating the removal of a side-chain ethanolamine-phosphate (EtNP) from the second Man (Man2) of the GPI intermediate, an essential step for efficient transport of GPI-anchor proteins.2 Publications
Cofactori
Mn2+1 PublicationNote: Binds 2 manganese ions per subunit.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 77 | Divalent metal cation 2Curated | 1 | |
Metal bindingi | 119 | Divalent metal cation 1Curated | 1 | |
Metal bindingi | 119 | Divalent metal cation 2Curated | 1 | |
Metal bindingi | 157 | Divalent metal cation 1Curated | 1 | |
Metal bindingi | 249 | Divalent metal cation 1Curated | 1 | |
Metal bindingi | 303 | Divalent metal cation 1Curated | 1 | |
Metal bindingi | 305 | Divalent metal cation 2Curated | 1 |
GO - Molecular functioni
- GPI anchor binding Source: UniProtKB
- GPI-mannose ethanolamine phosphate phosphodiesterase activity Source: UniProtKB
- manganese ion binding Source: UniProtKB
GO - Biological processi
- endoplasmic reticulum to Golgi vesicle-mediated transport Source: UniProtKB
- GPI anchor biosynthetic process Source: UniProtKB
Keywordsi
Molecular function | Hydrolase |
Biological process | ER-Golgi transport, GPI-anchor biosynthesis, Transport |
Ligand | Manganese, Metal-binding |
Enzyme and pathway databases
PathwayCommonsi | Q53F39 |
Names & Taxonomyi
Protein namesi | Recommended name: Metallophosphoesterase 1Curated (EC:3.1.-.-)Alternative name(s): Post-GPI attachment to proteins factor 51 Publication |
Gene namesi | ORF Names:PP579 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000154889.16 |
HGNCi | HGNC:15988, MPPE1 |
MIMi | 611900, gene |
neXtProti | NX_Q53F39 |
Subcellular locationi
Golgi apparatus
- cis-Golgi network membrane 1 Publication; Multi-pass membrane protein 1 Publication
Other locations
- Endoplasmic reticulum-Golgi intermediate compartment membrane 1 Publication; Multi-pass membrane protein 1 Publication
Note: Also localizes to endoplasmic reticulum exit site.
Endoplasmic reticulum
- endoplasmic reticulum exit site Source: UniProtKB
Golgi apparatus
- cis-Golgi network Source: UniProtKB
- Golgi apparatus Source: HPA
Nucleus
- nucleoplasm Source: HPA
Other locations
- endoplasmic reticulum-Golgi intermediate compartment Source: UniProtKB
- endoplasmic reticulum-Golgi intermediate compartment membrane Source: UniProtKB-SubCell
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 27 – 47 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 356 – 376 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Golgi apparatus, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 77 | D → A: Affects transport of GPI-anchor proteins. 1 Publication | 1 | |
Mutagenesisi | 79 | H → A: Affects transport of GPI-anchor proteins. 1 Publication | 1 | |
Mutagenesisi | 119 | D → A or N: Affects transport of GPI-anchor proteins. 1 Publication | 1 | |
Mutagenesisi | 157 | N → A: Affects transport of GPI-anchor proteins. 1 Publication | 1 | |
Mutagenesisi | 158 | H → A: Affects transport of GPI-anchor proteins. 1 Publication | 1 | |
Mutagenesisi | 249 | H → A: Affects transport of GPI-anchor proteins. 1 Publication | 1 | |
Mutagenesisi | 303 | H → A: Affects transport of GPI-anchor proteins. 1 Publication | 1 | |
Mutagenesisi | 305 | H → A: Affects transport of GPI-anchor proteins. 1 Publication | 1 | |
Mutagenesisi | 392 – 394 | KRK → ARA: Affects subcellular localization. 1 Publication | 3 |
Organism-specific databases
DisGeNETi | 65258 |
OpenTargetsi | ENSG00000154889 |
PharmGKBi | PA134913534 |
Miscellaneous databases
Pharosi | Q53F39, Tbio |
Polymorphism and mutation databases
BioMutai | MPPE1 |
DMDMi | 215274110 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000315727 | 1 – 396 | Metallophosphoesterase 1Add BLAST | 396 |
Proteomic databases
EPDi | Q53F39 |
jPOSTi | Q53F39 |
MassIVEi | Q53F39 |
MaxQBi | Q53F39 |
PaxDbi | Q53F39 |
PeptideAtlasi | Q53F39 |
PRIDEi | Q53F39 |
ProteomicsDBi | 62456 [Q53F39-1] 62457 [Q53F39-2] 62458 [Q53F39-3] 62459 [Q53F39-4] 62460 [Q53F39-5] |
PTM databases
GlyGeni | Q53F39, 1 site |
iPTMneti | Q53F39 |
PhosphoSitePlusi | Q53F39 |
Expressioni
Tissue specificityi
Expressed in brain.1 Publication
Gene expression databases
Bgeei | ENSG00000154889, Expressed in caudate nucleus and 234 other tissues |
ExpressionAtlasi | Q53F39, baseline and differential |
Genevisiblei | Q53F39, HS |
Organism-specific databases
HPAi | ENSG00000154889, Low tissue specificity |
Interactioni
Subunit structurei
Interacts with GPI-anchor proteins.
Interacts with TMED10.
1 PublicationBinary interactionsi
Hide detailsQ53F39
With | #Exp. | IntAct |
---|---|---|
FLJ13057 [Q53SE7] | 3 | EBI-10242229,EBI-10172181 |
ZNF426 [Q9BUY5] | 3 | EBI-10242229,EBI-743265 |
Protein-protein interaction databases
BioGRIDi | 122415, 80 interactors |
IntActi | Q53F39, 72 interactors |
STRINGi | 9606.ENSP00000465894 |
Miscellaneous databases
RNActi | Q53F39, protein |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 392 – 396 | Di-lysine motif | 5 |
Domaini
The di-lysine motif (KxKxx) acts as an endoplasmic reticulum retrieval signal.1 Publication
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3662, Eukaryota |
GeneTreei | ENSGT00390000013236 |
InParanoidi | Q53F39 |
OMAi | RTLHCMK |
OrthoDBi | 1094620at2759 |
PhylomeDBi | Q53F39 |
Family and domain databases
CDDi | cd08165, MPP_MPPE1, 1 hit |
Gene3Di | 3.60.21.10, 1 hit |
InterProi | View protein in InterPro IPR004843, Calcineurin-like_PHP_ApaH IPR029052, Metallo-depent_PP-like IPR039541, MPP_MPPE1 IPR033308, PGAP5/Cdc1/Ted1 |
PANTHERi | PTHR13315, PTHR13315, 1 hit |
Pfami | View protein in Pfam PF00149, Metallophos, 1 hit |
s (5+)i Sequence
Sequence statusi: Complete.
This entry describes 5 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 5 described isoforms and 14 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q53F39-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAMIELGFGR QNFHPLKRKS SLLLKLIAVV FAVLLFCEFL IYYLAIFQCN
60 70 80 90 100
WPEVKTTASD GEQTTREPVL KAMFLADTHL LGEFLGHWLD KLRREWQMER
110 120 130 140 150
AFQTALWLLQ PEVVFILGDI FDEGKWSTPE AWADDVERFQ KMFRHPSHVQ
160 170 180 190 200
LKVVAGNHDI GFHYEMNTYK VERFEKVFSS ERLFSWKGIN FVMVNSVALN
210 220 230 240 250
GDGCGICSET EAELIEVSHR LNCSREARGS SRCGPGPLLP TSAPVLLQHY
260 270 280 290 300
PLYRRSDANC SGEDAAPAEE RDIPFKENYD VLSREASQKL LWWLQPRLVL
310 320 330 340 350
SGHTHSACEV HHGGRVPELS VPSFSWRNRN NPSFIMGSIT PTDYTLSKCY
360 370 380 390
LPREDVVLII YCGVVGFLVV LTLTHFGLLA SPFLSGLNLL GKRKTR
Computationally mapped potential isoform sequencesi
There are 14 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A0A0MR93 | A0A0A0MR93_HUMAN | Metallophosphoesterase 1 | MPPE1 | 374 | Annotation score: | ||
K7ES91 | K7ES91_HUMAN | Metallophosphoesterase 1 | MPPE1 | 308 | Annotation score: | ||
K7EQV4 | K7EQV4_HUMAN | Metallophosphoesterase 1 | MPPE1 | 178 | Annotation score: | ||
K7EQ70 | K7EQ70_HUMAN | Metallophosphoesterase 1 | MPPE1 | 164 | Annotation score: | ||
A0A0C4DH11 | A0A0C4DH11_HUMAN | Metallophosphoesterase 1 | MPPE1 | 243 | Annotation score: | ||
K7EM03 | K7EM03_HUMAN | Metallophosphoesterase 1 | MPPE1 | 172 | Annotation score: | ||
K7EIQ0 | K7EIQ0_HUMAN | Metallophosphoesterase 1 | MPPE1 | 81 | Annotation score: | ||
K7ESB8 | K7ESB8_HUMAN | Metallophosphoesterase 1 | MPPE1 | 94 | Annotation score: | ||
K7EJU0 | K7EJU0_HUMAN | Metallophosphoesterase 1 | MPPE1 | 141 | Annotation score: | ||
K7ER13 | K7ER13_HUMAN | Metallophosphoesterase 1 | MPPE1 | 92 | Annotation score: | ||
There are more potential isoformsShow all |
Sequence cautioni
The sequence BAB13863 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
The sequence BAB14378 differs from that shown. Reason: Frameshift.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 22 | L → S in BAB14378 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 32 | A → T in BAB14378 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 97 | Q → R in AAL55744 (PubMed:15498874).Curated | 1 | |
Sequence conflicti | 301 | S → I in AAH73994 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 364 | V → E in AAM00279 (PubMed:11978971).Curated | 1 | |
Sequence conflicti | 364 | V → E in AAM00277 (PubMed:11978971).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_038294 | 138 | R → Q. Corresponds to variant dbSNP:rs11872520Ensembl. | 1 | |
Natural variantiVAR_038295 | 197 | V → M. Corresponds to variant dbSNP:rs35611363Ensembl. | 1 | |
Natural variantiVAR_038296 | 268 | A → P4 PublicationsCorresponds to variant dbSNP:rs662515Ensembl. | 1 | |
Natural variantiVAR_038297 | 336 | M → L. Corresponds to variant dbSNP:rs16976814Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_030679 | 226 | E → EQ in isoform 3. 1 Publication | 1 | |
Alternative sequenceiVSP_030680 | 227 – 289 | Missing in isoform 4. 1 PublicationAdd BLAST | 63 | |
Alternative sequenceiVSP_030681 | 227 – 256 | ARGSS…LYRRS → VGEHLNATGAFCPVLLRFGC SLSPLALLAL in isoform 5. 1 PublicationAdd BLAST | 30 | |
Alternative sequenceiVSP_030682 | 257 – 396 | Missing in isoform 5. 1 PublicationAdd BLAST | 140 | |
Alternative sequenceiVSP_030683 | 338 – 396 | SITPT…KRKTR → TDA in isoform 2 and isoform 3. 4 PublicationsAdd BLAST | 59 |
Sequence databases
Genome annotation databases
Ensembli | ENST00000309976; ENSP00000311200; ENSG00000154889 [Q53F39-4] ENST00000317235; ENSP00000327257; ENSG00000154889 [Q53F39-4] ENST00000496196; ENSP00000433950; ENSG00000154889 [Q53F39-2] ENST00000588072; ENSP00000465894; ENSG00000154889 [Q53F39-1] |
GeneIDi | 65258 |
KEGGi | hsa:65258 |
UCSCi | uc002kqf.5, human [Q53F39-1] |
Keywords - Coding sequence diversityi
Alternative splicing, PolymorphismSimilar proteinsi
Cross-referencesi
Sequence databases
3D structure databases
SMRi | Q53F39 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 122415, 80 interactors |
IntActi | Q53F39, 72 interactors |
STRINGi | 9606.ENSP00000465894 |
PTM databases
GlyGeni | Q53F39, 1 site |
iPTMneti | Q53F39 |
PhosphoSitePlusi | Q53F39 |
Polymorphism and mutation databases
BioMutai | MPPE1 |
DMDMi | 215274110 |
Proteomic databases
EPDi | Q53F39 |
jPOSTi | Q53F39 |
MassIVEi | Q53F39 |
MaxQBi | Q53F39 |
PaxDbi | Q53F39 |
PeptideAtlasi | Q53F39 |
PRIDEi | Q53F39 |
ProteomicsDBi | 62456 [Q53F39-1] 62457 [Q53F39-2] 62458 [Q53F39-3] 62459 [Q53F39-4] 62460 [Q53F39-5] |
Protocols and materials databases
Antibodypediai | 1617, 132 antibodies |
DNASUi | 65258 |
Genome annotation databases
Ensembli | ENST00000309976; ENSP00000311200; ENSG00000154889 [Q53F39-4] ENST00000317235; ENSP00000327257; ENSG00000154889 [Q53F39-4] ENST00000496196; ENSP00000433950; ENSG00000154889 [Q53F39-2] ENST00000588072; ENSP00000465894; ENSG00000154889 [Q53F39-1] |
GeneIDi | 65258 |
KEGGi | hsa:65258 |
UCSCi | uc002kqf.5, human [Q53F39-1] |
Organism-specific databases
CTDi | 65258 |
DisGeNETi | 65258 |
EuPathDBi | HostDB:ENSG00000154889.16 |
GeneCardsi | MPPE1 |
HGNCi | HGNC:15988, MPPE1 |
HPAi | ENSG00000154889, Low tissue specificity |
MIMi | 611900, gene |
neXtProti | NX_Q53F39 |
OpenTargetsi | ENSG00000154889 |
PharmGKBi | PA134913534 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3662, Eukaryota |
GeneTreei | ENSGT00390000013236 |
InParanoidi | Q53F39 |
OMAi | RTLHCMK |
OrthoDBi | 1094620at2759 |
PhylomeDBi | Q53F39 |
Enzyme and pathway databases
PathwayCommonsi | Q53F39 |
Miscellaneous databases
BioGRID-ORCSi | 65258, 38 hits in 843 CRISPR screens |
ChiTaRSi | MPPE1, human |
GenomeRNAii | 65258 |
Pharosi | Q53F39, Tbio |
PROi | PR:Q53F39 |
RNActi | Q53F39, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000154889, Expressed in caudate nucleus and 234 other tissues |
ExpressionAtlasi | Q53F39, baseline and differential |
Genevisiblei | Q53F39, HS |
Family and domain databases
CDDi | cd08165, MPP_MPPE1, 1 hit |
Gene3Di | 3.60.21.10, 1 hit |
InterProi | View protein in InterPro IPR004843, Calcineurin-like_PHP_ApaH IPR029052, Metallo-depent_PP-like IPR039541, MPP_MPPE1 IPR033308, PGAP5/Cdc1/Ted1 |
PANTHERi | PTHR13315, PTHR13315, 1 hit |
Pfami | View protein in Pfam PF00149, Metallophos, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MPPE1_HUMAN | |
Accessioni | Q53F39Primary (citable) accession number: Q53F39 Secondary accession number(s): B0YJ39 Q9HAI4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 15, 2008 |
Last sequence update: | November 25, 2008 | |
Last modified: | December 2, 2020 | |
This is version 122 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families - Human chromosome 18
Human chromosome 18: entries, gene names and cross-references to MIM - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations