UniProtKB - Q53F19 (NCBP3_HUMAN)
Protein
Nuclear cap-binding protein subunit 3
Gene
NCBP3
Organism
Homo sapiens (Human)
Status
Functioni
Associates with NCBP1/CBP80 to form an alternative cap-binding complex (CBC) which plays a key role in mRNA export. NCBP3 serves as adapter protein linking the capped RNAs (m7GpppG-capped RNA) to NCBP1/CBP80. Unlike the conventional CBC with NCBP2 which binds both small nuclear RNA (snRNA) and messenger (mRNA) and is involved in their export from the nucleus, the alternative CBC with NCBP3 does not bind snRNA and associates only with mRNA thereby playing a role in only mRNA export. The alternative CBC is particularly important in cellular stress situations such as virus infections and the NCBP3 activity is critical to inhibit virus growth (PubMed:26382858).1 Publication
GO - Molecular functioni
- mRNA binding Source: UniProtKB
- RNA 7-methylguanosine cap binding Source: UniProtKB
- RNA binding Source: UniProtKB
- RNA cap binding Source: GO_Central
GO - Biological processi
- 7-methylguanosine mRNA capping Source: UniProtKB-KW
- defense response to virus Source: UniProtKB
- mRNA transport Source: UniProtKB-KW
Keywordsi
Molecular function | RNA-binding |
Biological process | Antiviral defense, mRNA capping, mRNA processing, mRNA transport, Transport |
Enzyme and pathway databases
PathwayCommonsi | Q53F19 |
Names & Taxonomyi
Protein namesi | Recommended name: Nuclear cap-binding protein subunit 3ImportedAlternative name(s): Protein ELG |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:24612, NCBP3 |
MIMi | 616624, gene |
neXtProti | NX_Q53F19 |
VEuPathDBi | HostDB:ENSG00000074356.16 |
Subcellular locationi
Nucleus
- nuclear speck Source: HPA
- nucleus Source: UniProtKB
Other locations
- cytoplasm Source: UniProtKB
- RNA cap binding complex Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 134 | D → A: Minor loss of 7-methylguanosine-containing mRNA cap binding. 1 Publication | 1 | |
Mutagenesisi | 155 – 158 | WLDD → ALAA: Complete loss of 7-methylguanosine-containing mRNA cap binding. 1 Publication | 4 |
Organism-specific databases
OpenTargetsi | ENSG00000074356 |
PharmGKBi | PA145149545 |
Miscellaneous databases
Pharosi | Q53F19, Tbio |
Genetic variation databases
BioMutai | NCBP3 |
DMDMi | 160017360 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000308582 | 1 – 620 | Nuclear cap-binding protein subunit 3Add BLAST | 620 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 12 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Modified residuei | 25 | PhosphoserineCombined sources | 1 | |
Cross-linki | 70 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Modified residuei | 73 | PhosphoserineCombined sources | 1 | |
Cross-linki | 186 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Modified residuei | 209 | PhosphoserineCombined sources | 1 | |
Modified residuei | 210 | PhosphoserineCombined sources | 1 | |
Modified residuei | 413 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 415 | PhosphoserineCombined sources | 1 | |
Cross-linki | 541 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Modified residuei | 620 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q53F19 |
jPOSTi | Q53F19 |
MassIVEi | Q53F19 |
MaxQBi | Q53F19 |
PaxDbi | Q53F19 |
PeptideAtlasi | Q53F19 |
PRIDEi | Q53F19 |
ProteomicsDBi | 62454 [Q53F19-1] 62455 [Q53F19-2] |
PTM databases
iPTMneti | Q53F19 |
PhosphoSitePlusi | Q53F19 |
Expressioni
Gene expression databases
Bgeei | ENSG00000074356, Expressed in testis and 216 other tissues |
ExpressionAtlasi | Q53F19, baseline and differential |
Genevisiblei | Q53F19, HS |
Organism-specific databases
HPAi | ENSG00000074356, Low tissue specificity |
Interactioni
Subunit structurei
Component of an alternative cap-binding complex (CBC) composed of NCBP1/CBP80 and NCBP3.
Interacts with SRRT, KPNA3, THOC5 and EIF4A3.
1 PublicationBinary interactionsi
Hide detailsQ53F19
With | #Exp. | IntAct |
---|---|---|
KPNA4 [O00629] | 5 | EBI-6657994,EBI-396343 |
LENG8 [Q96PV6] | 7 | EBI-6657994,EBI-739546 |
NCBP1 [Q09161] | 11 | EBI-6657994,EBI-464743 |
Protein-protein interaction databases
BioGRIDi | 120662, 73 interactors |
ComplexPortali | CPX-3624, Alternative nuclear cap-binding complex |
IntActi | Q53F19, 299 interactors |
MINTi | Q53F19 |
STRINGi | 9606.ENSP00000373657 |
Miscellaneous databases
RNActi | Q53F19, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 126 – 187 | RNA recognition motif (RRM) domain1 PublicationAdd BLAST | 62 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 155 – 158 | WLDD motif; essential for 7-methylguanosine-containing mRNA cap binding1 Publication | 4 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 340 – 376 | Glu-richAdd BLAST | 37 |
Sequence similaritiesi
Belongs to the NCBP3 family.Curated
Phylogenomic databases
eggNOGi | ENOG502QRX4, Eukaryota |
GeneTreei | ENSGT00390000005712 |
HOGENOMi | CLU_488838_0_0_1 |
InParanoidi | Q53F19 |
OMAi | QHTRPRP |
OrthoDBi | 535499at2759 |
PhylomeDBi | Q53F19 |
TreeFami | TF331339 |
Family and domain databases
Gene3Di | 3.30.70.330, 1 hit |
InterProi | View protein in InterPro IPR019416, NCBP3 IPR012677, Nucleotide-bd_a/b_plait_sf |
PANTHERi | PTHR16291, PTHR16291, 1 hit |
Pfami | View protein in Pfam PF10309, NCBP3, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q53F19-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAAVRGLRVS VKAEAPAGPA LGLPSPEAES GVDRGEPEPM EVEEGELEIV
60 70 80 90 100
PVRRSLKELI PDTSRRYENK AGSFITGIDV TSKEAIEKKE QRAKRFHFRS
110 120 130 140 150
EVNLAQRNVA LDRDMMKKAI PKVRLETIYI CGVDEMSTQD VFSYFKEYPP
160 170 180 190 200
AHIEWLDDTS CNVVWLDEMT ATRALINMSS LPAQDKIRSR DASEDKSAEK
210 220 230 240 250
RKKDKQEDSS DDDEAEEGEV EDENSSDVEL DTLSQVEEES LLRNDLRPAN
260 270 280 290 300
KLAKGNRLFM RFATKDDKKE LGAARRSQYY MKYGNPNYGG MKGILSNSWK
310 320 330 340 350
RRYHSRRIQR DVIKKRALIG DDVGLTSYKH RHSGLVNVPE EPIEEEEEEE
360 370 380 390 400
EEEEEEEEED QDMDADDRVV VEYHEELPAL KQPRERSASR RSSASSSDSD
410 420 430 440 450
EMDYDLELKM ISTPSPKKSM KMTMYADEVE SQLKNIRNSM RADSVSSSNI
460 470 480 490 500
KNRIGNKLPP EKFADVRHLL DEKRQHSRPR PPVSSTKSDI RQRLGKRPHS
510 520 530 540 550
PEKAFSSNPV VRREPSSDVH SRLGVPRQDS KGLYADTREK KSGNLWTRLG
560 570 580 590 600
SAPKTKEKNT KKVDHRAPGA EEDDSELQRA WGALIKEKEQ SRQKKSRLDN
610 620
LPSLQIEVSR ESSSGSEAES
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketK7ELP6 | K7ELP6_HUMAN | Nuclear cap-binding protein subunit... | NCBP3 | 80 | Annotation score: | ||
K7EQA5 | K7EQA5_HUMAN | Nuclear cap-binding protein subunit... | NCBP3 | 36 | Annotation score: |
Sequence cautioni
The sequence BAD97190 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 465 | D → N in BAD97190 (Ref. 5) Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_028999 | 1 – 280 | Missing in isoform 2. 2 PublicationsAdd BLAST | 280 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ277841 mRNA Translation: CAB91001.1 AC005940 Genomic DNA No translation available. AC116914 Genomic DNA No translation available. AK125048 mRNA Translation: BAG54129.1 AK223470 mRNA Translation: BAD97190.1 Different initiation. CH471108 Genomic DNA Translation: EAW90479.1 BC010707 mRNA Translation: AAH10707.1 |
CCDSi | CCDS45578.1 [Q53F19-1] |
RefSeqi | NP_001107590.1, NM_001114118.2 [Q53F19-1] XP_016880318.1, XM_017024829.1 [Q53F19-2] |
Genome annotation databases
Ensembli | ENST00000389005; ENSP00000373657; ENSG00000074356 [Q53F19-1] |
GeneIDi | 55421 |
KEGGi | hsa:55421 |
UCSCi | uc010ckl.3, human [Q53F19-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ277841 mRNA Translation: CAB91001.1 AC005940 Genomic DNA No translation available. AC116914 Genomic DNA No translation available. AK125048 mRNA Translation: BAG54129.1 AK223470 mRNA Translation: BAD97190.1 Different initiation. CH471108 Genomic DNA Translation: EAW90479.1 BC010707 mRNA Translation: AAH10707.1 |
CCDSi | CCDS45578.1 [Q53F19-1] |
RefSeqi | NP_001107590.1, NM_001114118.2 [Q53F19-1] XP_016880318.1, XM_017024829.1 [Q53F19-2] |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 120662, 73 interactors |
ComplexPortali | CPX-3624, Alternative nuclear cap-binding complex |
IntActi | Q53F19, 299 interactors |
MINTi | Q53F19 |
STRINGi | 9606.ENSP00000373657 |
PTM databases
iPTMneti | Q53F19 |
PhosphoSitePlusi | Q53F19 |
Genetic variation databases
BioMutai | NCBP3 |
DMDMi | 160017360 |
Proteomic databases
EPDi | Q53F19 |
jPOSTi | Q53F19 |
MassIVEi | Q53F19 |
MaxQBi | Q53F19 |
PaxDbi | Q53F19 |
PeptideAtlasi | Q53F19 |
PRIDEi | Q53F19 |
ProteomicsDBi | 62454 [Q53F19-1] 62455 [Q53F19-2] |
Protocols and materials databases
Antibodypediai | 64617, 38 antibodies |
DNASUi | 55421 |
Genome annotation databases
Ensembli | ENST00000389005; ENSP00000373657; ENSG00000074356 [Q53F19-1] |
GeneIDi | 55421 |
KEGGi | hsa:55421 |
UCSCi | uc010ckl.3, human [Q53F19-1] |
Organism-specific databases
CTDi | 55421 |
GeneCardsi | NCBP3 |
HGNCi | HGNC:24612, NCBP3 |
HPAi | ENSG00000074356, Low tissue specificity |
MIMi | 616624, gene |
neXtProti | NX_Q53F19 |
OpenTargetsi | ENSG00000074356 |
PharmGKBi | PA145149545 |
VEuPathDBi | HostDB:ENSG00000074356.16 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502QRX4, Eukaryota |
GeneTreei | ENSGT00390000005712 |
HOGENOMi | CLU_488838_0_0_1 |
InParanoidi | Q53F19 |
OMAi | QHTRPRP |
OrthoDBi | 535499at2759 |
PhylomeDBi | Q53F19 |
TreeFami | TF331339 |
Enzyme and pathway databases
PathwayCommonsi | Q53F19 |
Miscellaneous databases
BioGRID-ORCSi | 55421, 27 hits in 874 CRISPR screens |
ChiTaRSi | NCBP3, human |
GenomeRNAii | 55421 |
Pharosi | Q53F19, Tbio |
PROi | PR:Q53F19 |
RNActi | Q53F19, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000074356, Expressed in testis and 216 other tissues |
ExpressionAtlasi | Q53F19, baseline and differential |
Genevisiblei | Q53F19, HS |
Family and domain databases
Gene3Di | 3.30.70.330, 1 hit |
InterProi | View protein in InterPro IPR019416, NCBP3 IPR012677, Nucleotide-bd_a/b_plait_sf |
PANTHERi | PTHR16291, PTHR16291, 1 hit |
Pfami | View protein in Pfam PF10309, NCBP3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NCBP3_HUMAN | |
Accessioni | Q53F19Primary (citable) accession number: Q53F19 Secondary accession number(s): B3KWG7 Q9NXZ4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 23, 2007 |
Last sequence update: | October 23, 2007 | |
Last modified: | February 10, 2021 | |
This is version 126 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 17
Human chromosome 17: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families