Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

UniProtKB - Q53F19 (NCBP3_HUMAN)

(max 400 entries)x

Your basket is currently empty. i

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Nuclear cap-binding protein subunit 3

Gene

NCBP3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Associates with NCBP1/CBP80 to form an alternative cap-binding complex (CBC) which plays a key role in mRNA export. NCBP3 serves as adapter protein linking the capped RNAs (m7GpppG-capped RNA) to NCBP1/CBP80. Unlike the conventional CBC with NCBP2 which binds both small nuclear RNA (snRNA) and messenger (mRNA) and is involved in their export from the nucleus, the alternative CBC with NCBP3 does not bind snRNA and associates only with mRNA thereby playing a role in only mRNA export. The alternative CBC is particularly important in cellular stress situations such as virus infections and the NCBP3 activity is critical to inhibit virus growth (PubMed:26382858).1 Publication

GO - Molecular functioni

  • mRNA binding Source: UniProtKB
  • RNA 7-methylguanosine cap binding Source: UniProtKB
  • RNA binding Source: UniProtKB
View the complete GO annotation on QuickGO ...

GO - Biological processi

  • 7-methylguanosine mRNA capping Source: UniProtKB-KW
  • defense response to virus Source: UniProtKB
  • mRNA transport Source: UniProtKB-KW
View the complete GO annotation on QuickGO ...

Keywordsi

Molecular functionRNA-binding
Biological processAntiviral defense, mRNA capping, mRNA processing, mRNA transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear cap-binding protein subunit 3Imported
Alternative name(s):
Protein ELG
Gene namesi
Name:NCBP3Imported
Synonyms:C17orf85
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

EuPathDBiHostDB:ENSG00000074356.16
HGNCiHGNC:24612 NCBP3
MIMi616624 gene
neXtProtiNX_Q53F19

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi134D → A: Minor loss of 7-methylguanosine-containing mRNA cap binding. 1 Publication1
Mutagenesisi155 – 158WLDD → ALAA: Complete loss of 7-methylguanosine-containing mRNA cap binding. 1 Publication4

Organism-specific databases

OpenTargetsiENSG00000074356
PharmGKBiPA145149545

Polymorphism and mutation databases

BioMutaiC17orf85
DMDMi160017360

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003085821 – 620Nuclear cap-binding protein subunit 3Add BLAST620

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki12Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei25PhosphoserineCombined sources1
Cross-linki70Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei73PhosphoserineCombined sources1
Cross-linki186Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei209PhosphoserineCombined sources1
Modified residuei210PhosphoserineCombined sources1
Modified residuei413PhosphothreonineCombined sources1
Modified residuei415PhosphoserineCombined sources1
Cross-linki541Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei620PhosphoserineCombined sources1

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ53F19
MaxQBiQ53F19
PaxDbiQ53F19
PeptideAtlasiQ53F19
PRIDEiQ53F19
ProteomicsDBi62454
62455 [Q53F19-2]

PTM databases

iPTMnetiQ53F19
PhosphoSitePlusiQ53F19

Expressioni

Gene expression databases

BgeeiENSG00000074356
CleanExiHS_C17orf85
ExpressionAtlasiQ53F19 baseline and differential
GenevisibleiQ53F19 HS

Organism-specific databases

HPAiHPA008959
HPA013195

Interactioni

Subunit structurei

Component of an alternative cap-binding complex (CBC) composed of NCBP1/CBP80 and NCBP3. Interacts with SRRT, KPNA3, THOC5 and EIF4A3.1 Publication

Protein-protein interaction databases

BioGridi120662, 60 interactors
IntActiQ53F19, 29 interactors
MINTiQ53F19
STRINGi9606.ENSP00000373657

Structurei

3D structure databases

ProteinModelPortaliQ53F19
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni126 – 187RNA recognition motif (RRM) domain1 PublicationAdd BLAST62

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi155 – 158WLDD motif; essential for 7-methylguanosine-containing mRNA cap binding1 Publication4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi340 – 376Glu-richAdd BLAST37

Sequence similaritiesi

Belongs to the NCBP3 family.Curated

Phylogenomic databases

eggNOGiENOG410IHKG Eukaryota
ENOG4110MQP LUCA
GeneTreeiENSGT00390000005712
HOGENOMiHOG000059260
HOVERGENiHBG059983
InParanoidiQ53F19
OMAiQDIFSYF
OrthoDBiEOG091G0ELU
PhylomeDBiQ53F19
TreeFamiTF331339

Family and domain databases

Gene3Di3.30.70.330, 1 hit
InterProiView protein in InterPro
IPR019416 NCBP3
IPR012677 Nucleotide-bd_a/b_plait_sf
PANTHERiPTHR16291 PTHR16291, 1 hit
PfamiView protein in Pfam
PF10309 NCBP3, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q53F19-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAVRGLRVS VKAEAPAGPA LGLPSPEAES GVDRGEPEPM EVEEGELEIV 
        60         70         80         90        100
PVRRSLKELI PDTSRRYENK AGSFITGIDV TSKEAIEKKE QRAKRFHFRS 
       110        120        130        140        150
EVNLAQRNVA LDRDMMKKAI PKVRLETIYI CGVDEMSTQD VFSYFKEYPP 
       160        170        180        190        200
AHIEWLDDTS CNVVWLDEMT ATRALINMSS LPAQDKIRSR DASEDKSAEK 
       210        220        230        240        250
RKKDKQEDSS DDDEAEEGEV EDENSSDVEL DTLSQVEEES LLRNDLRPAN 
       260        270        280        290        300
KLAKGNRLFM RFATKDDKKE LGAARRSQYY MKYGNPNYGG MKGILSNSWK 
       310        320        330        340        350
RRYHSRRIQR DVIKKRALIG DDVGLTSYKH RHSGLVNVPE EPIEEEEEEE 
       360        370        380        390        400
EEEEEEEEED QDMDADDRVV VEYHEELPAL KQPRERSASR RSSASSSDSD 
       410        420        430        440        450
EMDYDLELKM ISTPSPKKSM KMTMYADEVE SQLKNIRNSM RADSVSSSNI 
       460        470        480        490        500
KNRIGNKLPP EKFADVRHLL DEKRQHSRPR PPVSSTKSDI RQRLGKRPHS 
       510        520        530        540        550
PEKAFSSNPV VRREPSSDVH SRLGVPRQDS KGLYADTREK KSGNLWTRLG 
       560        570        580        590        600
SAPKTKEKNT KKVDHRAPGA EEDDSELQRA WGALIKEKEQ SRQKKSRLDN 
       610        620
LPSLQIEVSR ESSSGSEAES
Length:620
Mass (Da):70,593
Last modified:October 23, 2007 - v2
Checksum:i78ABD2F3E0BE4760
GO
Isoform 2 (identifier: Q53F19-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-280: Missing.

Show »
Length:340
Mass (Da):38,920
Checksum:i7C1D9D36C19FE9B2
GO

Sequence cautioni

The sequence BAD97190 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti465D → N in BAD97190 (Ref. 5) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0289991 – 280Missing in isoform 2. 2 PublicationsAdd BLAST280

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ277841 mRNA Translation: CAB91001.1
AC005940 Genomic DNA No translation available.
AC116914 Genomic DNA No translation available.
AK125048 mRNA Translation: BAG54129.1
AK223470 mRNA Translation: BAD97190.1 Different initiation.
CH471108 Genomic DNA Translation: EAW90479.1
BC010707 mRNA Translation: AAH10707.1
CCDSiCCDS45578.1 [Q53F19-1]
RefSeqiNP_001107590.1, NM_001114118.2 [Q53F19-1]
XP_016880318.1, XM_017024829.1 [Q53F19-2]
UniGeneiHs.120963

Genome annotation databases

EnsembliENST00000389005; ENSP00000373657; ENSG00000074356 [Q53F19-1]
GeneIDi55421
KEGGihsa:55421
UCSCiuc010ckl.3 human [Q53F19-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiNCBP3_HUMAN
AccessioniPrimary (citable) accession number: Q53F19
Secondary accession number(s): B3KWG7
, Q7L406, Q96FK1, Q9NXZ4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 23, 2007
Last sequence update: October 23, 2007
Last modified: June 20, 2018
This is version 108 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health