UniProtKB - Q53EZ4 (CEP55_HUMAN)
Centrosomal protein of 55 kDa
CEP55
Functioni
Plays a role in mitotic exit and cytokinesis (PubMed:16198290, PubMed:17853893).
Recruits PDCD6IP and TSG101 to midbody during cytokinesis. Required for successful completion of cytokinesis (PubMed:17853893).
Not required for microtubule nucleation (PubMed:16198290).
Plays a role in the development of the brain and kidney (PubMed:28264986).
3 PublicationsGO - Molecular functioni
- identical protein binding Source: IntAct
GO - Biological processi
- cranial skeletal system development Source: UniProtKB
- establishment of protein localization Source: UniProtKB
- midbody abscission Source: UniProtKB
- mitotic cytokinesis Source: MGI
- regulation of phosphatidylinositol 3-kinase signaling Source: InterPro
Keywordsi
Molecular function | Developmental protein |
Biological process | Cell cycle, Cell division, Mitosis |
Enzyme and pathway databases
PathwayCommonsi | Q53EZ4 |
SignaLinki | Q53EZ4 |
SIGNORi | Q53EZ4 |
Names & Taxonomyi
Protein namesi | Recommended name: Centrosomal protein of 55 kDaImportedShort name: Cep55 Alternative name(s): Up-regulated in colon cancer 6 |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:1161, CEP55 |
MIMi | 610000, gene |
neXtProti | NX_Q53EZ4 |
VEuPathDBi | HostDB:ENSG00000138180 |
Subcellular locationi
Cytoskeleton
- centriole 1 Publication
- centrosome 1 Publication
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Other locations
- Cleavage furrow 1 Publication
- Midbody ring 5 Publications
Note: Present at the centrosomes at interphase. A small portion is associated preferentially with the mother centriole, whereas the majority localizes to the pericentriolar material. During mitosis, loses affinity for the centrosome at the onset of prophase and diffuses throughout the cell. This dissociation from the centrosome is phosphorylation-dependent. May remain localized at the centrosome during mitosis in certain cell types. Appears at the cleavage furrow in late anaphase and in the midbody in cytokinesis.1 Publication
Cytoskeleton
- centriolar satellite Source: HPA
- centriole Source: UniProtKB-SubCell
- centrosome Source: UniProtKB
Extracellular region or secreted
- intercellular bridge Source: Ensembl
Plasma Membrane
- cleavage furrow Source: UniProtKB-SubCell
- plasma membrane Source: HPA
Other locations
- cytoplasm Source: UniProtKB-SubCell
- Flemming body Source: UniProtKB
- membrane Source: UniProtKB
- midbody Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, CytoskeletonPathology & Biotechi
Involvement in diseasei
Multinucleated neurons, anhydramnios, renal dysplasia, cerebellar hypoplasia and hydranencephaly (MARCH)2 Publications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_079363 | 86 – 464 | Missing in MARCH. 1 PublicationAdd BLAST | 379 | |
Natural variantiVAR_079364 | 425 – 464 | Missing in MARCH; loss of protein localization to midbody ring; loss of function; fails to rescue craniofacial development when expressed in a zebrafish heterologous system. 1 PublicationAdd BLAST | 40 |
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 184 | W → A: Abolishes interaction with PDCD6IP. 1 Publication | 1 | |
Mutagenesisi | 187 | Y → A: Abolishes interaction with PDCD6IP. 1 Publication | 1 | |
Mutagenesisi | 188 | D → A: Diminishes interaction with PDCD6IP. 1 Publication | 1 | |
Mutagenesisi | 191 | R → A: Abolishes interaction with PDCD6IP. 1 Publication | 1 | |
Mutagenesisi | 192 | E → A: Abolishes interaction with PDCD6IP. 1 Publication | 1 | |
Mutagenesisi | 396 | S → A: No effect on phosphorylation in mitotic cells. 1 Publication | 1 | |
Mutagenesisi | 425 | S → A: Partial loss of phosphorylation in mitotic cells. Complete loss of phosphorylation in mitotic cells; when associated with A-428. Remains associated with the centrosome throughout mitosis; when associated with A-428. Arrests mitotic cells at the midbody stage; when associated with A-428 and A-436. 1 Publication | 1 | |
Mutagenesisi | 428 | S → A: Partial loss of phosphorylation in mitotic cells. Complete loss of phosphorylation in mitotic cells; when associated with A-425. Remains associated with the centrosome throughout mitosis; when associated with A-425. Arrests mitotic cells at the midbody stage; when associated with A-425 and A-436. 1 Publication | 1 | |
Mutagenesisi | 436 | S → A: No effect on phosphorylation in mitotic cells. Arrests mitotic cells at the midbody stage; when associated with A-425 and A-428. 1 Publication | 1 |
Keywords - Diseasei
Disease variantOrganism-specific databases
DisGeNETi | 55165 |
MalaCardsi | CEP55 |
MIMi | 236500, phenotype |
OpenTargetsi | ENSG00000138180 |
Orphaneti | 500135, Multinucleated neurons-anhydramnios-renal dysplasia-cerebellar hypoplasia-hydranencephaly syndrome |
PharmGKBi | PA25475 |
Miscellaneous databases
Pharosi | Q53EZ4, Tbio |
Genetic variation databases
BioMutai | CEP55 |
DMDMi | 296439403 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000089777 | 1 – 464 | Centrosomal protein of 55 kDaAdd BLAST | 464 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 96 | PhosphoserineBy similarity | 1 | |
Modified residuei | 425 | Phosphoserine; by CDK1 and MAPK1Combined sources1 Publication | 1 | |
Modified residuei | 428 | Phosphoserine; by CDK1 and MAPK1Combined sources1 Publication | 1 | |
Modified residuei | 430 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 436 | Phosphoserine; by PLK1Combined sources1 Publication | 1 |
Post-translational modificationi
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q53EZ4 |
jPOSTi | Q53EZ4 |
MassIVEi | Q53EZ4 |
MaxQBi | Q53EZ4 |
PaxDbi | Q53EZ4 |
PeptideAtlasi | Q53EZ4 |
PRIDEi | Q53EZ4 |
ProteomicsDBi | 62452 [Q53EZ4-1] 62453 [Q53EZ4-2] |
PTM databases
iPTMneti | Q53EZ4 |
PhosphoSitePlusi | Q53EZ4 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000138180, Expressed in female gonad and 140 other tissues |
ExpressionAtlasi | Q53EZ4, baseline and differential |
Genevisiblei | Q53EZ4, HS |
Organism-specific databases
HPAi | ENSG00000138180, Tissue enhanced (lymphoid tissue, testis) |
Interactioni
Subunit structurei
Homodimer (PubMed:16406728).
Interacts (phosphorylated on Ser-425 and Ser-428) with PLK1 (PubMed:16198290).
Interacts with AKAP9/CG-NAP; the interaction occurs in interphase and is lost upon mitotic entry (PubMed:16198290).
Interacts with PCNT/Kendrin; the interaction occurs in interphase and is lost upon mitotic entry (PubMed:16198290). Directly interacts with PDCD6IP; this interaction is required for PDCD6IP targeting to the midbody; CEP55 binds PDCD6IP in a 2:1 stoichiometry; PDCD6IP competes with TSG101 for the same binding site (PubMed:17853893, PubMed:17556548, PubMed:18641129, PubMed:18948538).
Interacts with TSG101; TSG101 competes with PDCD6IP for the same binding site; interaction is required for cytokinesis but not for viral budding (PubMed:17853893, PubMed:17556548, PubMed:18948538).
Interacts with MVB12A, VPS37B, VPS37C and VPS28 (PubMed:17853893).
6 PublicationsBinary interactionsi
Q53EZ4
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 120465, 211 interactors |
CORUMi | Q53EZ4 |
DIPi | DIP-44581N |
ELMi | Q53EZ4 |
IntActi | Q53EZ4, 128 interactors |
MINTi | Q53EZ4 |
STRINGi | 9606.ENSP00000360540 |
Miscellaneous databases
RNActi | Q53EZ4, protein |
Structurei
Secondary structure
3D structure databases
SMRi | Q53EZ4 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q53EZ4 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 26 | DisorderedSequence analysisAdd BLAST | 26 | |
Regioni | 157 – 236 | Interaction with TSG101Add BLAST | 80 | |
Regioni | 160 – 214 | Interaction with PDCD6IPAdd BLAST | 55 | |
Regioni | 355 – 464 | Required for localization to the interphase centrosome and to the midbody during cytokinesis1 PublicationAdd BLAST | 110 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 22 – 186 | Sequence analysisAdd BLAST | 165 | |
Coiled coili | 238 – 337 | Sequence analysisAdd BLAST | 100 | |
Coiled coili | 374 – 403 | Sequence analysisAdd BLAST | 30 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 1 – 23 | Polar residuesSequence analysisAdd BLAST | 23 |
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | ENOG502QTDI, Eukaryota |
GeneTreei | ENSGT00510000047961 |
HOGENOMi | CLU_047132_0_0_1 |
InParanoidi | Q53EZ4 |
OMAi | HIDFCTA |
OrthoDBi | 850483at2759 |
PhylomeDBi | Q53EZ4 |
TreeFami | TF331107 |
Family and domain databases
InterProi | View protein in InterPro IPR038926, CEP55 IPR022008, EABR |
PANTHERi | PTHR31838, PTHR31838, 1 hit |
Pfami | View protein in Pfam PF12180, EABR, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSSRSTKDLI KSKWGSKPSN SKSETTLEKL KGEIAHLKTS VDEITSGKGK
60 70 80 90 100
LTDKERHRLL EKIRVLEAEK EKNAYQLTEK DKEIQRLRDQ LKARYSTTTL
110 120 130 140 150
LEQLEETTRE GERREQVLKA LSEEKDVLKQ QLSAATSRIA ELESKTNTLR
160 170 180 190 200
LSQTVAPNCF NSSINNIHEM EIQLKDALEK NQQWLVYDQQ REVYVKGLLA
210 220 230 240 250
KIFELEKKTE TAAHSLPQQT KKPESEGYLQ EEKQKCYNDL LASAKKDLEV
260 270 280 290 300
ERQTITQLSF ELSEFRRKYE ETQKEVHNLN QLLYSQRRAD VQHLEDDRHK
310 320 330 340 350
TEKIQKLREE NDIARGKLEE EKKRSEELLS QVQFLYTSLL KQQEEQTRVA
360 370 380 390 400
LLEQQMQACT LDFENEKLDR QHVQHQLHVI LKELRKARNQ ITQLESLKQL
410 420 430 440 450
HEFAITEPLV TFQGETENRE KVAASPKSPT AALNESLVEC PKCNIQYPAT
460
EHRDLLVHVE YCSK
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketH0Y432 | H0Y432_HUMAN | Centrosomal protein of 55 kDa | CEP55 | 208 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 155 | V → A in BAD97215 (Ref. 4) Curated | 1 | |
Sequence conflicti | 204 | E → G in BAD97215 (Ref. 4) Curated | 1 | |
Sequence conflicti | 298 | R → G in CAE45837 (PubMed:17974005).Curated | 1 | |
Sequence conflicti | 334 | F → S in AAX14687 (PubMed:16406728).Curated | 1 | |
Sequence conflicti | 334 | F → S in BAA91670 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 435 | E → G in AAX14687 (PubMed:16406728).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_026559 | 57 | H → Q No effect on protein localization to midbody ring. 3 PublicationsCorresponds to variant dbSNP:rs3740370Ensembl. | 1 | |
Natural variantiVAR_079363 | 86 – 464 | Missing in MARCH. 1 PublicationAdd BLAST | 379 | |
Natural variantiVAR_022996 | 99 | T → ACombined sources7 PublicationsCorresponds to variant dbSNP:rs7080916Ensembl. | 1 | |
Natural variantiVAR_056791 | 236 | C → R. Corresponds to variant dbSNP:rs7072484Ensembl. | 1 | |
Natural variantiVAR_022997 | 378 | H → L No effect on protein localization to midbody ring; rescues craniofacial development when expressed in a zebrafish heterologous system. 5 PublicationsCorresponds to variant dbSNP:rs2293277Ensembl. | 1 | |
Natural variantiVAR_079364 | 425 – 464 | Missing in MARCH; loss of protein localization to midbody ring; loss of function; fails to rescue craniofacial development when expressed in a zebrafish heterologous system. 1 PublicationAdd BLAST | 40 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_014750 | 389 – 400 | NQITQ…SLKQL → KNNTVGILETAS in isoform 2. 1 PublicationAdd BLAST | 12 | |
Alternative sequenceiVSP_014751 | 401 – 464 | Missing in isoform 2. 1 PublicationAdd BLAST | 64 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY788918 mRNA Translation: AAX14687.1 AB091343 mRNA Translation: BAE45243.1 AK001402 mRNA Translation: BAA91670.1 Different initiation. AK315536 mRNA Translation: BAG37915.1 AK223495 mRNA Translation: BAD97215.1 BX640718 mRNA Translation: CAE45837.1 AL356214 Genomic DNA No translation available. CH471066 Genomic DNA Translation: EAW50071.1 CH471066 Genomic DNA Translation: EAW50072.1 BC008947 mRNA Translation: AAH08947.1 |
CCDSi | CCDS7428.1 [Q53EZ4-1] |
RefSeqi | NP_001120654.1, NM_001127182.1 [Q53EZ4-1] NP_060601.3, NM_018131.4 [Q53EZ4-1] |
Genome annotation databases
Ensembli | ENST00000371485; ENSP00000360540; ENSG00000138180 |
GeneIDi | 55165 |
KEGGi | hsa:55165 |
MANE-Selecti | ENST00000371485.8; ENSP00000360540.3; NM_018131.5; NP_060601.4 |
UCSCi | uc001kiq.4, human [Q53EZ4-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY788918 mRNA Translation: AAX14687.1 AB091343 mRNA Translation: BAE45243.1 AK001402 mRNA Translation: BAA91670.1 Different initiation. AK315536 mRNA Translation: BAG37915.1 AK223495 mRNA Translation: BAD97215.1 BX640718 mRNA Translation: CAE45837.1 AL356214 Genomic DNA No translation available. CH471066 Genomic DNA Translation: EAW50071.1 CH471066 Genomic DNA Translation: EAW50072.1 BC008947 mRNA Translation: AAH08947.1 |
CCDSi | CCDS7428.1 [Q53EZ4-1] |
RefSeqi | NP_001120654.1, NM_001127182.1 [Q53EZ4-1] NP_060601.3, NM_018131.4 [Q53EZ4-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3E1R | X-ray | 2.00 | A/B | 160-217 | [»] | |
3WUT | X-ray | 2.30 | A/B/D/E/G/H/J/K | 160-217 | [»] | |
3WUU | X-ray | 2.90 | A/B/D/E/G/H/J/K | 160-217 | [»] | |
3WUV | X-ray | 2.79 | A/B/D/E/G/H/J/K/M/N/P/Q | 160-217 | [»] | |
SMRi | Q53EZ4 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 120465, 211 interactors |
CORUMi | Q53EZ4 |
DIPi | DIP-44581N |
ELMi | Q53EZ4 |
IntActi | Q53EZ4, 128 interactors |
MINTi | Q53EZ4 |
STRINGi | 9606.ENSP00000360540 |
PTM databases
iPTMneti | Q53EZ4 |
PhosphoSitePlusi | Q53EZ4 |
Genetic variation databases
BioMutai | CEP55 |
DMDMi | 296439403 |
Proteomic databases
EPDi | Q53EZ4 |
jPOSTi | Q53EZ4 |
MassIVEi | Q53EZ4 |
MaxQBi | Q53EZ4 |
PaxDbi | Q53EZ4 |
PeptideAtlasi | Q53EZ4 |
PRIDEi | Q53EZ4 |
ProteomicsDBi | 62452 [Q53EZ4-1] 62453 [Q53EZ4-2] |
Protocols and materials databases
Antibodypediai | 30468, 319 antibodies from 32 providers |
DNASUi | 55165 |
Genome annotation databases
Ensembli | ENST00000371485; ENSP00000360540; ENSG00000138180 |
GeneIDi | 55165 |
KEGGi | hsa:55165 |
MANE-Selecti | ENST00000371485.8; ENSP00000360540.3; NM_018131.5; NP_060601.4 |
UCSCi | uc001kiq.4, human [Q53EZ4-1] |
Organism-specific databases
CTDi | 55165 |
DisGeNETi | 55165 |
GeneCardsi | CEP55 |
HGNCi | HGNC:1161, CEP55 |
HPAi | ENSG00000138180, Tissue enhanced (lymphoid tissue, testis) |
MalaCardsi | CEP55 |
MIMi | 236500, phenotype 610000, gene |
neXtProti | NX_Q53EZ4 |
OpenTargetsi | ENSG00000138180 |
Orphaneti | 500135, Multinucleated neurons-anhydramnios-renal dysplasia-cerebellar hypoplasia-hydranencephaly syndrome |
PharmGKBi | PA25475 |
VEuPathDBi | HostDB:ENSG00000138180 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502QTDI, Eukaryota |
GeneTreei | ENSGT00510000047961 |
HOGENOMi | CLU_047132_0_0_1 |
InParanoidi | Q53EZ4 |
OMAi | HIDFCTA |
OrthoDBi | 850483at2759 |
PhylomeDBi | Q53EZ4 |
TreeFami | TF331107 |
Enzyme and pathway databases
PathwayCommonsi | Q53EZ4 |
SignaLinki | Q53EZ4 |
SIGNORi | Q53EZ4 |
Miscellaneous databases
BioGRID-ORCSi | 55165, 188 hits in 1052 CRISPR screens |
ChiTaRSi | CEP55, human |
EvolutionaryTracei | Q53EZ4 |
GeneWikii | CEP55 |
GenomeRNAii | 55165 |
Pharosi | Q53EZ4, Tbio |
PROi | PR:Q53EZ4 |
RNActi | Q53EZ4, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000138180, Expressed in female gonad and 140 other tissues |
ExpressionAtlasi | Q53EZ4, baseline and differential |
Genevisiblei | Q53EZ4, HS |
Family and domain databases
InterProi | View protein in InterPro IPR038926, CEP55 IPR022008, EABR |
PANTHERi | PTHR31838, PTHR31838, 1 hit |
Pfami | View protein in Pfam PF12180, EABR, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CEP55_HUMAN | |
Accessioni | Q53EZ4Primary (citable) accession number: Q53EZ4 Secondary accession number(s): B2RDG8 Q9NVS7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 19, 2005 |
Last sequence update: | May 18, 2010 | |
Last modified: | February 23, 2022 | |
This is version 160 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 10
Human chromosome 10: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references