UniProtKB - Q53563 (MMOC_METTR)
Protein
Methane monooxygenase component C
Gene
mmoC
Organism
Methylosinus trichosporium
Status
Functioni
Responsible for the initial oxygenation of methane to methanol in methanotrophs. It also catalyzes the monohydroxylation of a variety of unactivated alkenes, alicyclic, aromatic and heterocyclic compounds. The component C is the iron-sulfur flavoprotein of sMMO.
Catalytic activityi
- EC:1.14.13.25
- EC:1.14.13.25
Cofactori
[2Fe-2S] clusterNote: Binds 1 [2Fe-2S] cluster.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 37 | Iron-sulfur (2Fe-2S) | 1 | |
Metal bindingi | 41 | Iron-sulfur (2Fe-2S) | 1 | |
Metal bindingi | 44 | Iron-sulfur (2Fe-2S) | 1 | |
Metal bindingi | 76 | Iron-sulfur (2Fe-2S) | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 215 – 229 | FADBy similarityAdd BLAST | 15 |
GO - Molecular functioni
- 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
- electron transfer activity Source: InterPro
- metal ion binding Source: UniProtKB-KW
- methane monooxygenase activity Source: UniProtKB-EC
GO - Biological processi
- one-carbon metabolic process Source: UniProtKB-KW
Keywordsi
Molecular function | Monooxygenase, Oxidoreductase |
Biological process | Electron transport, One-carbon metabolism, Transport |
Ligand | 2Fe-2S, FAD, Flavoprotein, Iron, Iron-sulfur, Metal-binding, NADP |
Enzyme and pathway databases
BioCyci | MetaCyc:MONOMER-3870 |
Names & Taxonomyi
Protein namesi | Recommended name: Methane monooxygenase component C (EC:1.14.13.25)Alternative name(s): Methane hydroxylase Methane monooxygenase reductase Short name: MMOR |
Gene namesi | Name:mmoC |
Organismi | Methylosinus trichosporium |
Taxonomic identifieri | 426 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Methylocystaceae › Methylosinus |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000189409 | 1 – 340 | Methane monooxygenase component CAdd BLAST | 340 |
Interactioni
Subunit structurei
The soluble methane monooxygenase (sMMO) consists of four components A/MMOH (composed of alpha/MmoX, beta/MmoY and gamma/MmoZ), B/MMOB (MmoB), C/MMOR (MmoC) and D/MMOD (MmoD).
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1 – 92 | 2Fe-2S ferredoxin-typeAdd BLAST | 92 | |
Domaini | 101 – 205 | FAD-binding FR-typePROSITE-ProRule annotationAdd BLAST | 105 |
Family and domain databases
CDDi | cd00207, fer2, 1 hit |
Gene3Di | 3.10.20.30, 1 hit 3.40.50.80, 1 hit |
InterProi | View protein in InterPro IPR036010, 2Fe-2S_ferredoxin-like_sf IPR001041, 2Fe-2S_ferredoxin-type IPR012675, Beta-grasp_dom_sf IPR008333, Cbr1-like_FAD-bd_dom IPR017927, FAD-bd_FR_type IPR001709, Flavoprot_Pyr_Nucl_cyt_Rdtase IPR039261, FNR_nucleotide-bd IPR001433, OxRdtase_FAD/NAD-bd IPR017938, Riboflavin_synthase-like_b-brl |
Pfami | View protein in Pfam PF00970, FAD_binding_6, 1 hit PF00111, Fer2, 1 hit PF00175, NAD_binding_1, 1 hit |
PRINTSi | PR00371, FPNCR |
SUPFAMi | SSF52343, SSF52343, 1 hit SSF54292, SSF54292, 1 hit SSF63380, SSF63380, 1 hit |
PROSITEi | View protein in PROSITE PS51384, FAD_FR, 1 hit |
i Sequence
Sequence statusi: Complete.
Q53563-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MYQIVIETED GETCRRMRPS EDWISRAEAE RNLLASCRAG CATCKADCTD
60 70 80 90 100
GDYELIDVKV QAVPPDEEED GKVLLCRTFP RSDLHLLVPY TYDRISFEAI
110 120 130 140 150
QTNWLAEILA CDRVSSNVVR LVLQRSRPMA ARISLNFVPG QFVDIEIPGT
160 170 180 190 200
HTRRSYSMAS VAEDGQLEFI IRLLPDGAFS KFLQTEAKVG MRVDLRGPAG
210 220 230 240 250
SFFLHDHGGR SRVFVAGGTG LSPVLSMIRQ LGKASDPSPA TLLFGVTNRE
260 270 280 290 300
ELFYVDELKT LAQSMPTLGV RIAVVNDDGG NGVDKGTVID LLRAELEIDL
310 320 330 340
LLGHARRRRR RETARSCRED HRDRCPAWRS DFLEKFLASG
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | S81887 Genomic DNA Translation: AAB21393.1 |
PIRi | C48360 |
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | S81887 Genomic DNA Translation: AAB21393.1 |
PIRi | C48360 |
3D structure databases
SMRi | Q53563 |
ModBasei | Search... |
Enzyme and pathway databases
BioCyci | MetaCyc:MONOMER-3870 |
Family and domain databases
CDDi | cd00207, fer2, 1 hit |
Gene3Di | 3.10.20.30, 1 hit 3.40.50.80, 1 hit |
InterProi | View protein in InterPro IPR036010, 2Fe-2S_ferredoxin-like_sf IPR001041, 2Fe-2S_ferredoxin-type IPR012675, Beta-grasp_dom_sf IPR008333, Cbr1-like_FAD-bd_dom IPR017927, FAD-bd_FR_type IPR001709, Flavoprot_Pyr_Nucl_cyt_Rdtase IPR039261, FNR_nucleotide-bd IPR001433, OxRdtase_FAD/NAD-bd IPR017938, Riboflavin_synthase-like_b-brl |
Pfami | View protein in Pfam PF00970, FAD_binding_6, 1 hit PF00111, Fer2, 1 hit PF00175, NAD_binding_1, 1 hit |
PRINTSi | PR00371, FPNCR |
SUPFAMi | SSF52343, SSF52343, 1 hit SSF54292, SSF54292, 1 hit SSF63380, SSF63380, 1 hit |
PROSITEi | View protein in PROSITE PS51384, FAD_FR, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MMOC_METTR | |
Accessioni | Q53563Primary (citable) accession number: Q53563 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 6, 2002 |
Last sequence update: | November 1, 1996 | |
Last modified: | February 26, 2020 | |
This is version 103 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |