Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Entry version 88 (02 Dec 2020)
Sequence version 1 (01 Nov 1996)
Previous versions | rss
Add a publicationFeedback
Protein

4-hydroxybenzoate transporter PcaK

Gene

pcaK

Organism
Pseudomonas putida (Arthrobacter siderocapsulatus)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Transports 4-hydroxybenzoate (4-HBA) and protocatechuate across the membrane. Driven by the proton motive force. Functions also as a chemoreceptor, which is required for chemotaxis to aromatic acids.

2 Publications

<p>This subsection of the 'Function' section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

  1. KM=6 µM for 4-hydroxybenzoate1 Publication
  1. Vmax=25 nmol/min/mg enzyme with 4-hydroxybenzoate as substrate1 Publication

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Biological processChemotaxis, Transport

Protein family/group databases

Transport Classification Database

More...
TCDBi
2.A.1.15.1, the major facilitator superfamily (mfs)

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
4-hydroxybenzoate transporter PcaK
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:pcaK
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiPseudomonas putida (Arthrobacter siderocapsulatus)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri303 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular%5Flocation%5Fsection">'Subcellular location'</a> section describes the subcellular compartment where each non-membrane region of a membrane-spanning protein is found.<p><a href='/help/topo_dom' target='_top'>More...</a></p>Topological domaini1 – 30CytoplasmicSequence analysisAdd BLAST30
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular%5Flocation%5Fsection">'Subcellular location'</a> section describes the extent of a membrane-spanning region of the protein. It denotes the presence of both alpha-helical transmembrane regions and the membrane spanning regions of beta-barrel transmembrane proteins.<p><a href='/help/transmem' target='_top'>More...</a></p>Transmembranei31 – 51Helical; Name=1Sequence analysisAdd BLAST21
Topological domaini52 – 67PeriplasmicSequence analysisAdd BLAST16
Transmembranei68 – 88Helical; Name=2Sequence analysisAdd BLAST21
Topological domaini89 – 94CytoplasmicSequence analysis6
Transmembranei95 – 115Helical; Name=3Sequence analysisAdd BLAST21
Topological domaini116 – 119PeriplasmicSequence analysis4
Transmembranei120 – 140Helical; Name=4Sequence analysisAdd BLAST21
Topological domaini141 – 152CytoplasmicSequence analysisAdd BLAST12
Transmembranei153 – 173Helical; Name=5Sequence analysisAdd BLAST21
Topological domaini174 – 184PeriplasmicSequence analysisAdd BLAST11
Transmembranei185 – 205Helical; Name=6Sequence analysisAdd BLAST21
Topological domaini206 – 261CytoplasmicSequence analysisAdd BLAST56
Transmembranei262 – 282Helical; Name=7Sequence analysisAdd BLAST21
Topological domaini283 – 301PeriplasmicSequence analysisAdd BLAST19
Transmembranei302 – 322Helical; Name=8Sequence analysisAdd BLAST21
Topological domaini323 – 329CytoplasmicSequence analysis7
Transmembranei330 – 350Helical; Name=9Sequence analysisAdd BLAST21
Topological domaini351PeriplasmicSequence analysis1
Transmembranei352 – 372Helical; Name=10Sequence analysisAdd BLAST21
Topological domaini373 – 398CytoplasmicSequence analysisAdd BLAST26
Transmembranei399 – 419Helical; Name=11Sequence analysisAdd BLAST21
Topological domaini420 – 421PeriplasmicSequence analysis2
Transmembranei422 – 442Helical; Name=12Sequence analysisAdd BLAST21
Topological domaini443 – 448CytoplasmicSequence analysis6

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology%5Fand%5Fbiotech%5Fsection">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi41D → A or N: Abolishes 4-HBA transport. 1 Publication1
Mutagenesisi41D → E: Decrease in 4-HBA transport. 1 Publication1
Mutagenesisi44D → A or N: Abolishes 4-HBA transport. 1 Publication1
Mutagenesisi44D → E: Decrease in 4-HBA transport. 1 Publication1
Mutagenesisi85G → V: Abolishes 4-HBA transport and chemotaxis. 1 Publication1
Mutagenesisi89D → N: Abolishes 4-HBA transport and chemotaxis. 1 Publication1
Mutagenesisi92G → A: Decrease in 4-HBA transport and chemotaxis. 1 Publication1
Mutagenesisi92G → C: No change in 4-HBA transport and chemotaxis. 1 Publication1
Mutagenesisi92G → L or V: Abolishes 4-HBA transport and chemotaxis. 1 Publication1
Mutagenesisi92G → Q: Decrease in 4-HBA transport and strong decrease in chemotaxis. 1 Publication1
Mutagenesisi124R → A: Abolishes 4-HBA transport. 1 Publication1
Mutagenesisi144E → A: Strong decrease in 4-HBA transport. 1 Publication1
Mutagenesisi183H → A: Decrease in 4-HBA transport and chemotaxis. 1 Publication1
Mutagenesisi323D → N: Abolishes 4-HBA transport and chemotaxis. 1 Publication1
Mutagenesisi328H → A: Decrease in 4-HBA transport and chemotaxis. 1 Publication1
Mutagenesisi328H → R: Decrease in 4-HBA transport and loss of chemotaxis. 1 Publication1
Mutagenesisi386R → A: Strong decrease in 4-HBA transport. 1 Publication1
Mutagenesisi398R → A: Abolishes 4-HBA transport. 1 Publication1
Mutagenesisi444H → A: No change in 4-HBA transport and chemotaxis. 1 Publication1

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00000503211 – 4484-hydroxybenzoate transporter PcaKAdd BLAST448

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

STRING: functional protein association networks

More...
STRINGi
1240350.AMZE01000001_gene2837

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the 'Family and domains' section provides general information on the biological role of a domain. The term 'domain' is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

Amino acids located between the second and third, and the eighth and ninth transmembrane regions are required for substrate transport.1 Publication

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
COG2814, Bacteria

Family and domain databases

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR011701, MFS
IPR004746, MFS_AAHS
IPR020846, MFS_dom
IPR036259, MFS_trans_sf
IPR005829, Sugar_transporter_CS

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF07690, MFS_1, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF103473, SSF103473, 1 hit

TIGRFAMs; a protein family database

More...
TIGRFAMsi
TIGR00895, 2A0115, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS50850, MFS, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

Q51955-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MNQAQNSVGK SLDVQSFINQ QPLSRYQWRV VLLCFLIVFL DGLDTAAMGF
60 70 80 90 100
IAPALSQEWG IDRASLGPVM SAALIGMVFG ALGSGPLADR FGRKGVLVGA
110 120 130 140 150
VLVFGGFSLA SAYATNVDQL LVLRFLTGLG LGAGMPNATT LLSEYTPERL
160 170 180 190 200
KSLLVTSMFC GFNLGMAGGG FISAKMIPAY GWHSLLVIGG VLPLLLALVL
210 220 230 240 250
MVWLPESARF LVVRNRGTDK IRKTLSPIAP QVVAEAGSFS VPEQKAVAAR
260 270 280 290 300
SVFAVIFSGT YGLGTMLLWL TYFMGLVIVY LLTSWLPTLM RDSGASMEQA
310 320 330 340 350
AFIGALFQFG GVLSAVGVGW AMDRYNPHKV IGIFYLLAGV FAYAVGQSLG
360 370 380 390 400
NITVLATLVL IAGMCVNGAQ SAMPSLAARF YPTQGRATGV SWMLGIGRFG
410 420 430 440
AILGAWSGAT LLGLGWNFEQ VLTALLVPAA LATVGVIVKG LVSHADAT
Length:448
Mass (Da):47,177
Last modified:November 1, 1996 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i6D51C143123E99BC
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
U10895 Genomic DNA Translation: AAA85137.1

NCBI Reference Sequences

More...
RefSeqi
WP_016498119.1, NZ_UGUX01000003.1

Genome annotation databases

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
45522452

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10895 Genomic DNA Translation: AAA85137.1
RefSeqiWP_016498119.1, NZ_UGUX01000003.1

3D structure databases

Database of comparative protein structure models

More...
ModBasei
Search...

SWISS-MODEL Interactive Workspace

More...
SWISS-MODEL-Workspacei
Submit a new modelling project...

Protein-protein interaction databases

STRINGi1240350.AMZE01000001_gene2837

Protein family/group databases

TCDBi2.A.1.15.1, the major facilitator superfamily (mfs)

Genome annotation databases

GeneIDi45522452

Phylogenomic databases

eggNOGiCOG2814, Bacteria

Family and domain databases

InterProiView protein in InterPro
IPR011701, MFS
IPR004746, MFS_AAHS
IPR020846, MFS_dom
IPR036259, MFS_trans_sf
IPR005829, Sugar_transporter_CS
PfamiView protein in Pfam
PF07690, MFS_1, 1 hit
SUPFAMiSSF103473, SSF103473, 1 hit
TIGRFAMsiTIGR00895, 2A0115, 1 hit
PROSITEiView protein in PROSITE
PS50850, MFS, 1 hit

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiPCAK_PSEPU
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q51955
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: November 1, 1996
Last modified: December 2, 2020
This is version 88 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again