UniProtKB - Q51422 (SYDND_PSEAE)
Protein
Aspartate--tRNA(Asp/Asn) ligase
Gene
aspS
Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Functioni
Aspartyl-tRNA synthetase with relaxed tRNA specificity since it is able to aspartylate not only its cognate tRNA(Asp) but also tRNA(Asn). Is 1.5 times more efficient at aminoacylating E.coli tRNA(Asp) over tRNA(Asn). Reaction proceeds in two steps: L-aspartate is first activated by ATP to form Asp-AMP and then transferred to the acceptor end of tRNA(Asp/Asn).1 Publication
Catalytic activityi
- EC:6.1.1.23UniRule annotation1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 31 | Important for tRNA non-discrimination | 1 | |
Sitei | 82 | Important for tRNA non-discrimination | 1 | |
Binding sitei | 174 | AspartateUniRule annotation | 1 | |
Binding sitei | 220 | AspartateUniRule annotation | 1 | |
Binding sitei | 229 | ATPUniRule annotation | 1 | |
Binding sitei | 450 | AspartateUniRule annotation | 1 | |
Binding sitei | 483 | ATPUniRule annotation | 1 | |
Binding sitei | 490 | AspartateUniRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 220 – 222 | ATPUniRule annotation | 3 | |
Nucleotide bindingi | 535 – 538 | ATPUniRule annotation | 4 |
GO - Molecular functioni
- aspartate-tRNA(Asn) ligase activity Source: UniProtKB-EC
- aspartate-tRNA ligase activity Source: UniProtKB-UniRule
- ATP binding Source: UniProtKB-UniRule
- nucleic acid binding Source: InterPro
GO - Biological processi
- aspartyl-tRNA aminoacylation Source: UniProtKB-UniRule
Keywordsi
Molecular function | Aminoacyl-tRNA synthetase, Ligase |
Biological process | Protein biosynthesis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | PAER208964:G1FZ6-984-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Aspartate--tRNA(Asp/Asn) ligaseUniRule annotation (EC:6.1.1.23UniRule annotation)Alternative name(s): Aspartyl-tRNA synthetaseUniRule annotation Short name: AspRSUniRule annotation Non-discriminating aspartyl-tRNA synthetaseUniRule annotation Short name: ND-AspRSUniRule annotation |
Gene namesi | Name:aspSUniRule annotation Ordered Locus Names:PA0963 |
Organismi | Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) |
Taxonomic identifieri | 208964 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › |
Proteomesi |
|
Organism-specific databases
PseudoCAPi | PA0963 |
Subcellular locationi
- Cytoplasm UniRule annotation
GO - Cellular componenti
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 31 | H → L: Enhances enzyme specificity for tRNA(Asp) over tRNA(Asn) by 3.5-fold, by reducing enzyme's ability to misacylate tRNA(Asn) when tested against E.coli tRNA, but shows little effect when tested against P.aeruginosa tRNA. 1 Publication | 1 | |
Mutagenesisi | 82 | G → K: Enhances enzyme specificity for tRNA(Asp) over tRNA(Asn) by 4.2-fold, by reducing enzyme's ability to misacylate tRNA(Asn) when tested against E.coli tRNA, but shows little effect when tested against P.aeruginosa tRNA. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000110924 | 1 – 591 | Aspartate--tRNA(Asp/Asn) ligaseAdd BLAST | 591 |
Proteomic databases
PaxDbi | Q51422 |
PRIDEi | Q51422 |
Interactioni
Subunit structurei
Homodimer.
UniRule annotationStructurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q51422 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 198 – 201 | AspartateUniRule annotation | 4 |
Sequence similaritiesi
Phylogenomic databases
HOGENOMi | CLU_014330_3_2_6 |
InParanoidi | Q51422 |
OMAi | YQLDVEM |
PhylomeDBi | Q51422 |
Family and domain databases
Gene3Di | 3.30.1360.30, 1 hit |
HAMAPi | MF_00044, Asp_tRNA_synth_type1, 1 hit |
InterProi | View protein in InterPro IPR004364, Aa-tRNA-synt_II IPR006195, aa-tRNA-synth_II IPR004524, Asp-tRNA-ligase_1 IPR002312, Asp/Asn-tRNA-synth_IIb IPR004115, GAD-like_sf IPR029351, GAD_dom IPR012340, NA-bd_OB-fold IPR004365, NA-bd_OB_tRNA |
PANTHERi | PTHR22594:SF5, PTHR22594:SF5, 1 hit |
Pfami | View protein in Pfam PF02938, GAD, 1 hit PF00152, tRNA-synt_2, 1 hit PF01336, tRNA_anti-codon, 1 hit |
PRINTSi | PR01042, TRNASYNTHASP |
SUPFAMi | SSF50249, SSF50249, 1 hit SSF55261, SSF55261, 1 hit |
TIGRFAMsi | TIGR00459, aspS_bact, 1 hit |
PROSITEi | View protein in PROSITE PS50862, AA_TRNA_LIGASE_II, 1 hit |
i Sequence
Sequence statusi: Complete.
Q51422-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MMRSHYCGQL NESLDGQEVT LCGWVHRRRD HGGVIFLDVR DREGLAQVVF
60 70 80 90 100
DPDRAETFAK ADRVRSEFVV KITGKVRLRP EGARNPNMAS GSIEVLGYEL
110 120 130 140 150
EVLNQAETPP FPLDEYSDVG EETRLRYRFI DLRRPEMAAK LKLRARITSS
160 170 180 190 200
IRRYLDDNGF LDVETPILGR PTPEGARDYL VPSRTYPGHF FALPQSPQLF
210 220 230 240 250
KQLLMVAGFD RYYQIAKCFR DEDLRADRQP EFTQIDIETS FLDESDIIGI
260 270 280 290 300
TEKMVRQLFK EVLDVEFDEF PHMPFEEAMR RYGSDKPDLR IPLELVDVAD
310 320 330 340 350
QLKEVEFKVF SGPANDPKGR VAALRVPGAA SMPRSQIDDY TKFVGIYGAK
360 370 380 390 400
GLAYIKVNER AKGVEGLQSP IVKFIPEANL NVILDRVGAV DGDIVFFGAD
410 420 430 440 450
KAKIVCDALG ALRIKVGHDL KLLTREWAPM WVVDFPMFEE NDDGSLSALH
460 470 480 490 500
HPFTSPKCTP AELEANPGAA LSRAYDMVLN GTELGGGSIR IHDKSMQQAV
510 520 530 540 550
FRVLGIDEAE QEEKFGFLLD ALKYGAPPHG GLAFGLDRLV MLMTGASSIR
560 570 580 590
EVIAFPKTQS AGDVMTQAPG SVDGKALREL HIRLREQPKA E
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 588 | P → A in BAA11815 (PubMed:8982068).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE004091 Genomic DNA Translation: AAG04352.1 D83138 Genomic DNA Translation: BAA11815.1 |
PIRi | E83524 PC4295 |
RefSeqi | NP_249654.1, NC_002516.2 WP_003123218.1, NZ_QZGE01000007.1 |
Genome annotation databases
EnsemblBacteriai | AAG04352; AAG04352; PA0963 |
GeneIDi | 45711899 881917 |
KEGGi | pae:PA0963 |
PATRICi | fig|208964.12.peg.1001 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE004091 Genomic DNA Translation: AAG04352.1 D83138 Genomic DNA Translation: BAA11815.1 |
PIRi | E83524 PC4295 |
RefSeqi | NP_249654.1, NC_002516.2 WP_003123218.1, NZ_QZGE01000007.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4WJ3 | X-ray | 3.70 | M/N/O/P | 1-591 | [»] | |
4WJ4 | X-ray | 3.29 | A | 1-591 | [»] | |
SMRi | Q51422 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Proteomic databases
PaxDbi | Q51422 |
PRIDEi | Q51422 |
Genome annotation databases
EnsemblBacteriai | AAG04352; AAG04352; PA0963 |
GeneIDi | 45711899 881917 |
KEGGi | pae:PA0963 |
PATRICi | fig|208964.12.peg.1001 |
Organism-specific databases
PseudoCAPi | PA0963 |
Phylogenomic databases
HOGENOMi | CLU_014330_3_2_6 |
InParanoidi | Q51422 |
OMAi | YQLDVEM |
PhylomeDBi | Q51422 |
Enzyme and pathway databases
BioCyci | PAER208964:G1FZ6-984-MONOMER |
Family and domain databases
Gene3Di | 3.30.1360.30, 1 hit |
HAMAPi | MF_00044, Asp_tRNA_synth_type1, 1 hit |
InterProi | View protein in InterPro IPR004364, Aa-tRNA-synt_II IPR006195, aa-tRNA-synth_II IPR004524, Asp-tRNA-ligase_1 IPR002312, Asp/Asn-tRNA-synth_IIb IPR004115, GAD-like_sf IPR029351, GAD_dom IPR012340, NA-bd_OB-fold IPR004365, NA-bd_OB_tRNA |
PANTHERi | PTHR22594:SF5, PTHR22594:SF5, 1 hit |
Pfami | View protein in Pfam PF02938, GAD, 1 hit PF00152, tRNA-synt_2, 1 hit PF01336, tRNA_anti-codon, 1 hit |
PRINTSi | PR01042, TRNASYNTHASP |
SUPFAMi | SSF50249, SSF50249, 1 hit SSF55261, SSF55261, 1 hit |
TIGRFAMsi | TIGR00459, aspS_bact, 1 hit |
PROSITEi | View protein in PROSITE PS50862, AA_TRNA_LIGASE_II, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SYDND_PSEAE | |
Accessioni | Q51422Primary (citable) accession number: Q51422 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | December 8, 2000 | |
Last modified: | December 2, 2020 | |
This is version 139 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families