UniProtKB - Q50L43 (PA24D_MOUSE)
Protein
Cytosolic phospholipase A2 delta
Gene
Pla2g4d
Organism
Mus musculus (Mouse)
Status
Functioni
Calcium-dependent phospholipase A2 that selectively hydrolyzes glycerophospholipids in the sn-2 position (PubMed:15866882). Compared to its human ortholog, may have no preference for the fatty acid found at the sn-2 position (PubMed:15866882).1 Publication
Catalytic activityi
- a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1-acyl-sn-glycero-3-phosphocholine + a fatty acid + H+1 PublicationEC:3.1.1.41 PublicationThis reaction proceeds in the forward1 Publication direction.
- 1-hexadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphocholine + H2O = (5Z,8Z,11Z,14Z)-eicosatetraenoate + 1-hexadecanoyl-sn-glycero-3-phosphocholine + H+1 PublicationThis reaction proceeds in the forward1 Publication direction.
- 1-hexadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphocholine + H2O = (9Z,12Z)-octadecadienoate + 1-hexadecanoyl-sn-glycero-3-phosphocholine + H+1 PublicationThis reaction proceeds in the forward1 Publication direction.
- 1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + H2O = (9Z)-octadecenoate + 1-hexadecanoyl-sn-glycero-3-phosphocholine + H+By similarityThis reaction proceeds in the forwardBy similarity direction.
- 1-hexadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphoethanolamine + H2O = (5Z,8Z,11Z,14Z)-eicosatetraenoate + 1-hexadecanoyl-sn-glycero-3-phosphoethanolamine + H+1 PublicationThis reaction proceeds in the forward1 Publication direction.
- 1-hexadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphoethanolamine + H2O = (9Z,12Z)-octadecadienoate + 1-hexadecanoyl-sn-glycero-3-phosphoethanolamine + H+1 PublicationThis reaction proceeds in the forward1 Publication direction.
- 1-hexadecanoyl-sn-glycero-3-phosphocholine + H2O = H+ + hexadecanoate + sn-glycerol 3-phosphocholine1 PublicationThis reaction proceeds in the forward1 Publication direction.
Cofactori
Ca2+PROSITE-ProRule annotation
Activity regulationi
Stimulated by cytosolic Ca2+.1 Publication
Kineticsi
- Vmax=13.4 pmol/min/mg enzyme with 1-hexadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphocholine as substrate (at 37 degrees Celsius)1 Publication
- Vmax=8.1 pmol/min/mg enzyme with 1-hexadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphocholine as substrate (at 37 degrees Celsius)1 Publication
- Vmax=14.5 pmol/min/mg enzyme with 1-hexadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphoethanolamine as substrate (at 37 degrees Celsius)1 Publication
- Vmax=19.9 pmol/min/mg enzyme with 1-hexadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphoethanolamine as substrate (at 37 degrees Celsius)1 Publication
- Vmax=16.4 pmol/min/mg enzyme with 1-hexadecanoyl-sn-glycero-3-phosphocholine as substrate (at 37 degrees Celsius)1 Publication
: fatty acid metabolism Pathwayi
This protein is involved in the pathway fatty acid metabolism, which is part of Lipid metabolism.1 PublicationView all proteins of this organism that are known to be involved in the pathway fatty acid metabolism and in Lipid metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 47 | Calcium 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 47 | Calcium 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 53 | Calcium 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 103 | Calcium 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 103 | Calcium 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 105 | Calcium 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 105 | Calcium 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 111 | Calcium 2PROSITE-ProRule annotation | 1 | |
Active sitei | 370 | NucleophileBy similarity | 1 | |
Active sitei | 654 | Proton acceptorBy similarity | 1 |
GO - Molecular functioni
- calcium-dependent phospholipase A2 activity Source: UniProtKB
- calcium-dependent phospholipid binding Source: GO_Central
- calcium ion binding Source: GO_Central
- phospholipase A2 activity Source: MGI
- phospholipase A2 activity (consuming 1,2-dipalmitoylphosphatidylcholine) Source: UniProtKB-EC
- phospholipase A2 activity consuming 1,2-dioleoylphosphatidylethanolamine) Source: UniProtKB-EC
GO - Biological processi
- fatty acid metabolic process Source: UniProtKB-UniPathway
- glycerophospholipid catabolic process Source: UniProtKB
Keywordsi
Molecular function | Hydrolase |
Biological process | Lipid degradation, Lipid metabolism |
Ligand | Calcium, Metal-binding |
Enzyme and pathway databases
Reactomei | R-MMU-1482788, Acyl chain remodelling of PC R-MMU-1482801, Acyl chain remodelling of PS R-MMU-1482839, Acyl chain remodelling of PE R-MMU-1482922, Acyl chain remodelling of PI R-MMU-1482925, Acyl chain remodelling of PG R-MMU-1483115, Hydrolysis of LPC R-MMU-1483166, Synthesis of PA |
UniPathwayi | UPA00199 |
Names & Taxonomyi
Protein namesi | Recommended name: Cytosolic phospholipase A2 deltaCurated (EC:3.1.1.41 Publication)Short name: cPLA2-delta1 Publication Alternative name(s): Phospholipase A2 group IVD |
Gene namesi | Name:Pla2g4dImported |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1925640, Pla2g4d |
Subcellular locationi
Cytoplasm and Cytosol
- cytosol 1 Publication
Other locations
- Membrane 1 Publication; Peripheral membrane protein 1 Publication; Cytoplasmic side 1 Publication
Note: Translocates to perinuclear membranes that may correspond to endoplasmic reticulum or Golgi in a calcium-dependent fashion.1 Publication
Keywords - Cellular componenti
Cytoplasm, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000247024 | 1 – 825 | Cytosolic phospholipase A2 deltaAdd BLAST | 825 |
Proteomic databases
PaxDbi | Q50L43 |
PRIDEi | Q50L43 |
ProteomicsDBi | 294370 |
PTM databases
iPTMneti | Q50L43 |
PhosphoSitePlusi | Q50L43 |
Expressioni
Tissue specificityi
Weakly or not expressed in most tissues. Detected in placenta of 17.5 dpc embryos.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000070719, Expressed in oocyte and 47 other tissues |
Genevisiblei | Q50L43, MM |
Interactioni
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000092252 |
Miscellaneous databases
RNActi | Q50L43, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 14 – 133 | C2PROSITE-ProRule annotationAdd BLAST | 120 | |
Domaini | 281 – 825 | PLA2cPROSITE-ProRule annotationAdd BLAST | 545 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 339 – 340 | Substrate bindingBy similarity | 2 |
Domaini
The N-terminal C2 domain mediates the association with lipid membranes upon calcium binding. An additional second C2 domain may stand in between the C2 domain and the PLA2c domain.By similarity
Phylogenomic databases
eggNOGi | KOG1028, Eukaryota KOG1325, Eukaryota |
GeneTreei | ENSGT01000000214525 |
HOGENOMi | CLU_011663_0_0_1 |
InParanoidi | Q50L43 |
OMAi | NSSHPVW |
OrthoDBi | 302848at2759 |
TreeFami | TF325228 |
Family and domain databases
CDDi | cd04036, C2_cPLA2, 1 hit |
Gene3Di | 2.60.40.150, 1 hit |
InterProi | View protein in InterPro IPR016035, Acyl_Trfase/lysoPLipase IPR041847, C2_cPLA2 IPR000008, C2_dom IPR035892, C2_domain_sf IPR040723, cPLA2_C2 IPR002642, LysoPLipase_cat_dom |
Pfami | View protein in Pfam PF00168, C2, 1 hit PF18695, cPLA2_C2, 1 hit PF01735, PLA2_B, 1 hit |
SMARTi | View protein in SMART SM00239, C2, 1 hit SM00022, PLAc, 1 hit |
SUPFAMi | SSF52151, SSF52151, 1 hit |
PROSITEi | View protein in PROSITE PS50004, C2, 1 hit PS51210, PLA2C, 1 hit |
i Sequence
Sequence statusi: Complete.
Q50L43-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MWSGDRRVGM ESLSPERLHG HPYQEEASVF CRLTVKILEA RSLPRADLLS
60 70 80 90 100
QADPYVTVQL PTASGMKFKT QTVTNSSHPV WNETFSFLIQ SQVKNILELT
110 120 130 140 150
IYDEDVITKD DICFKVSYDV SEILPGQLLQ KTFSLNPQGP EELDVELLME
160 170 180 190 200
RTWDPPENLI TNNVLVAREL SHLDVSLDRA GNTAMAAGQD KLELELMLKG
210 220 230 240 250
SYEDTQTFFP DTAFTFSFHY MRGQDTELNG YLRGPRNSGW NSDTSVTPFN
260 270 280 290 300
VPLMSLAAGK EMTIDIPAMK APEGKLQLKT DCCPKELSVR LSYGLCPEEQ
310 320 330 340 350
AFLSRRKKVV AAALKQALQL DEDLNEDEVP VVGINAEGGG MRAMISLYGH
360 370 380 390 400
LLALQKLGLL DCVTYFSGIS GSTWTMAHLY RDPEWSQRDL EGPISHAREH
410 420 430 440 450
VAKTLLKEFL PEHLASYRQT LKLREEQGYT VTVADLWGLV LESKLHGQVT
460 470 480 490 500
DQKLSGQRAA LERGQNPLPL YLSLNVKENH LETLHFKEWV EFSPYEVGFL
510 520 530 540 550
KYGGFVPSEL FGSEFFMGRL MKRLPESQIC FLEGIWSNLF SVNLMDIWYD
560 570 580 590 600
ITYGKDSNNF PVDVRNSEKE FSGSAGTSSG VEAPWLESGT ALAQALKGFL
610 620 630 640 650
TGRPFHQRSA NFLHGLQLHR DYCNQRHFST WADCNLDDTP NQLTPQDPQL
660 670 680 690 700
CLIDAGCFMN SSCPSLFRPG RQVDLIISFN YNQSLPFKGL QQSEKYSRAR
710 720 730 740 750
GLPFPRVEPS PEDHSQPQEC YLFSDPTCPE APVVLHFPLV NDSFRDHSAP
760 770 780 790 800
GVRRSPDELK AGQVNLTGAA SPYFMYNMTY KNEDFDRLLQ LSDYNVQNNQ
810 820
GTILQALRTV LKRRASETRP LGVKT
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 222 | R → G in BAE39570 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 222 | R → G in BAE26807 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 482 | E → G in BAE39570 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 482 | E → G in BAE26807 (PubMed:16141072).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB195276 mRNA Translation: BAD98152.1 AK167492 mRNA Translation: BAE39570.1 AK145985 mRNA Translation: BAE26807.1 AL844608 Genomic DNA No translation available. |
CCDSi | CCDS16617.1 |
RefSeqi | NP_001019308.1, NM_001024137.1 |
Genome annotation databases
Ensembli | ENSMUST00000094665; ENSMUSP00000092252; ENSMUSG00000070719 |
GeneIDi | 78390 |
KEGGi | mmu:78390 |
UCSCi | uc008lvh.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB195276 mRNA Translation: BAD98152.1 AK167492 mRNA Translation: BAE39570.1 AK145985 mRNA Translation: BAE26807.1 AL844608 Genomic DNA No translation available. |
CCDSi | CCDS16617.1 |
RefSeqi | NP_001019308.1, NM_001024137.1 |
3D structure databases
SMRi | Q50L43 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000092252 |
PTM databases
iPTMneti | Q50L43 |
PhosphoSitePlusi | Q50L43 |
Proteomic databases
PaxDbi | Q50L43 |
PRIDEi | Q50L43 |
ProteomicsDBi | 294370 |
Protocols and materials databases
Antibodypediai | 23488, 72 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000094665; ENSMUSP00000092252; ENSMUSG00000070719 |
GeneIDi | 78390 |
KEGGi | mmu:78390 |
UCSCi | uc008lvh.1, mouse |
Organism-specific databases
CTDi | 283748 |
MGIi | MGI:1925640, Pla2g4d |
Phylogenomic databases
eggNOGi | KOG1028, Eukaryota KOG1325, Eukaryota |
GeneTreei | ENSGT01000000214525 |
HOGENOMi | CLU_011663_0_0_1 |
InParanoidi | Q50L43 |
OMAi | NSSHPVW |
OrthoDBi | 302848at2759 |
TreeFami | TF325228 |
Enzyme and pathway databases
UniPathwayi | UPA00199 |
Reactomei | R-MMU-1482788, Acyl chain remodelling of PC R-MMU-1482801, Acyl chain remodelling of PS R-MMU-1482839, Acyl chain remodelling of PE R-MMU-1482922, Acyl chain remodelling of PI R-MMU-1482925, Acyl chain remodelling of PG R-MMU-1483115, Hydrolysis of LPC R-MMU-1483166, Synthesis of PA |
Miscellaneous databases
BioGRID-ORCSi | 78390, 1 hit in 52 CRISPR screens |
PROi | PR:Q50L43 |
RNActi | Q50L43, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000070719, Expressed in oocyte and 47 other tissues |
Genevisiblei | Q50L43, MM |
Family and domain databases
CDDi | cd04036, C2_cPLA2, 1 hit |
Gene3Di | 2.60.40.150, 1 hit |
InterProi | View protein in InterPro IPR016035, Acyl_Trfase/lysoPLipase IPR041847, C2_cPLA2 IPR000008, C2_dom IPR035892, C2_domain_sf IPR040723, cPLA2_C2 IPR002642, LysoPLipase_cat_dom |
Pfami | View protein in Pfam PF00168, C2, 1 hit PF18695, cPLA2_C2, 1 hit PF01735, PLA2_B, 1 hit |
SMARTi | View protein in SMART SM00239, C2, 1 hit SM00022, PLAc, 1 hit |
SUPFAMi | SSF52151, SSF52151, 1 hit |
PROSITEi | View protein in PROSITE PS50004, C2, 1 hit PS51210, PLA2C, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PA24D_MOUSE | |
Accessioni | Q50L43Primary (citable) accession number: Q50L43 Secondary accession number(s): A2AQJ1, Q3TJC5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 25, 2006 |
Last sequence update: | July 27, 2011 | |
Last modified: | April 7, 2021 | |
This is version 117 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways