UniProtKB - Q50L41 (PA24F_MOUSE)
Protein
Cytosolic phospholipase A2 zeta
Gene
Pla2g4f
Organism
Mus musculus (Mouse)
Status
Functioni
Has calcium-dependent phospholipase and lysophospholipase activities with a potential role in membrane lipid remodeling and biosynthesis of lipid mediators (PubMed:17293613, PubMed:15866882). Preferentially hydrolyzes the ester bond of the fatty acyl group attached at sn-2 position of phospholipids (phospholipase A2 activity) (PubMed:17293613, PubMed:15866882). Selectively hydrolyzes sn-2 arachidonoyl group from membrane phospholipids, providing the precursor for eicosanoid biosynthesis (PubMed:15866882, PubMed:17293613). In myocardial mitochondria, plays a major role in arachidonate release that is metabolically channeled to the formation of cardioprotective eicosanoids, epoxyeicosatrienoates (EETs) (By similarity).By similarity2 Publications
Catalytic activityi
- a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1-acyl-sn-glycero-3-phosphocholine + a fatty acid + H+2 PublicationsEC:3.1.1.42 PublicationsThis reaction proceeds in the forward2 Publications direction.
- 1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + H2O = 2-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + H+ + hexadecanoate1 PublicationThis reaction proceeds in the forward1 Publication direction.
- 1-hexadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphocholine + H2O = (9Z,12Z)-octadecadienoate + 1-hexadecanoyl-sn-glycero-3-phosphocholine + H+1 PublicationThis reaction proceeds in the forward1 Publication direction.
- 1-hexadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphocholine + H2O = (5Z,8Z,11Z,14Z)-eicosatetraenoate + 1-hexadecanoyl-sn-glycero-3-phosphocholine + H+2 PublicationsThis reaction proceeds in the forward1 Publication direction.
- 1-hexadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphoethanolamine + H2O = (9Z,12Z)-octadecadienoate + 1-hexadecanoyl-sn-glycero-3-phosphoethanolamine + H+2 PublicationsThis reaction proceeds in the forward2 Publications direction.
- 1-hexadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphoethanolamine + H2O = (5Z,8Z,11Z,14Z)-eicosatetraenoate + 1-hexadecanoyl-sn-glycero-3-phosphoethanolamine + H+2 PublicationsThis reaction proceeds in the forward2 Publications direction.
- 1-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-2-O-hexadecyl-sn-glycero-3-phosphocholine + H2O = (5Z,8Z,11Z,14Z)-eicosatetraenoate + 2-O-hexadecyl-sn-glycero-3-phosphocholine + H+1 PublicationThis reaction proceeds in the forward1 Publication direction.
- 1-O-hexadecyl-2-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-sn-glycero-3-phosphocholine + H2O = (5Z,8Z,11Z,14Z)-eicosatetraenoate + 1-O-hexadecyl-sn-glycero-3-phosphocholine + H+1 PublicationThis reaction proceeds in the forward1 Publication direction.
- 1-hexadecanoyl-sn-glycero-3-phosphocholine + H2O = H+ + hexadecanoate + sn-glycerol 3-phosphocholine1 PublicationThis reaction proceeds in the forward1 Publication direction.
Cofactori
Ca2+PROSITE-ProRule annotation2 Publications
Activity regulationi
Stimulated by cytosolic Ca2+.2 Publications
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 60 | Calcium 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 60 | Calcium 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 66 | Calcium 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 116 | Calcium 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 116 | Calcium 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 118 | Calcium 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 118 | Calcium 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 123 | Calcium 2PROSITE-ProRule annotation | 1 | |
Active sitei | 393 | NucleophileBy similarity | 1 | |
Active sitei | 685 | Proton acceptorBy similarity | 1 |
GO - Molecular functioni
- calcium-dependent phospholipase A2 activity Source: MGI
- calcium-dependent phospholipid binding Source: GO_Central
- calcium ion binding Source: GO_Central
- lysophospholipase activity Source: MGI
- phospholipase A2 activity Source: MGI
- phospholipase A2 activity (consuming 1,2-dipalmitoylphosphatidylcholine) Source: UniProtKB-EC
- phospholipase A2 activity consuming 1,2-dioleoylphosphatidylethanolamine) Source: UniProtKB-EC
GO - Biological processi
- arachidonic acid secretion Source: MGI
- cellular response to antibiotic Source: MGI
- cellular response to organic cyclic compound Source: MGI
- fatty acid transport Source: MGI
- glycerophospholipid catabolic process Source: GO_Central
- prostaglandin biosynthetic process Source: MGI
Keywordsi
Molecular function | Hydrolase |
Biological process | Lipid degradation, Lipid metabolism, Phospholipid degradation, Phospholipid metabolism |
Ligand | Calcium, Metal-binding |
Enzyme and pathway databases
Reactomei | R-MMU-1482788, Acyl chain remodelling of PC R-MMU-1482801, Acyl chain remodelling of PS R-MMU-1482839, Acyl chain remodelling of PE R-MMU-1482922, Acyl chain remodelling of PI R-MMU-1482925, Acyl chain remodelling of PG R-MMU-1483115, Hydrolysis of LPC |
Chemistry databases
SwissLipidsi | SLP:000001085 |
Names & Taxonomyi
Protein namesi | Recommended name: Cytosolic phospholipase A2 zeta (EC:3.1.1.42 Publications)Short name: cPLA2-zeta Alternative name(s): Phospholipase A2 group IVF |
Gene namesi | Name:Pla2g4f |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2685493, Pla2g4f |
Subcellular locationi
Cytoplasm and Cytosol
- cytosol 2 Publications
Mitochondrion
- Mitochondrion By similarity
Plasma membrane
- Cell membrane 1 Publication; Peripheral membrane protein Curated
Cytosol
- cytosol Source: MGI
Mitochondrion
- mitochondrion Source: UniProtKB-SubCell
Plasma Membrane
- ruffle membrane Source: MGI
Other locations
Keywords - Cellular componenti
Cell membrane, Cytoplasm, Membrane, MitochondrionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000247028 | 1 – 855 | Cytosolic phospholipase A2 zetaAdd BLAST | 855 |
Proteomic databases
MaxQBi | Q50L41 |
PaxDbi | Q50L41 |
PRIDEi | Q50L41 |
ProteomicsDBi | 294373 |
PTM databases
PhosphoSitePlusi | Q50L41 |
Expressioni
Tissue specificityi
Strongly expressed in thyroid, expressed at intermediate level in stomach and at very low level in large intestine and prostate.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000046971, Expressed in skeletal muscle tissue and 133 other tissues |
Genevisiblei | Q50L41, MM |
Interactioni
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000062607 |
Miscellaneous databases
RNActi | Q50L41, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 27 – 145 | C2PROSITE-ProRule annotationAdd BLAST | 119 | |
Domaini | 304 – 855 | PLA2cPROSITE-ProRule annotationAdd BLAST | 552 |
Domaini
The N-terminal C2 domain associates with lipid membranes upon calcium binding. It modulates enzyme activity by presenting the active site to its substrate in response to elevations of cytosolic Ca2+ (By similarity).By similarity
Phylogenomic databases
eggNOGi | KOG1028, Eukaryota KOG1325, Eukaryota |
GeneTreei | ENSGT01000000214502 |
HOGENOMi | CLU_011663_0_0_1 |
InParanoidi | Q50L41 |
OMAi | GDFAEWC |
OrthoDBi | 302848at2759 |
TreeFami | TF325228 |
Family and domain databases
CDDi | cd04036, C2_cPLA2, 1 hit |
Gene3Di | 2.60.40.150, 1 hit |
InterProi | View protein in InterPro IPR016035, Acyl_Trfase/lysoPLipase IPR041847, C2_cPLA2 IPR000008, C2_dom IPR035892, C2_domain_sf IPR040723, cPLA2_C2 IPR002642, LysoPLipase_cat_dom |
Pfami | View protein in Pfam PF00168, C2, 1 hit PF18695, cPLA2_C2, 1 hit PF01735, PLA2_B, 1 hit |
SMARTi | View protein in SMART SM00239, C2, 1 hit SM00022, PLAc, 1 hit |
SUPFAMi | SSF52151, SSF52151, 1 hit |
PROSITEi | View protein in PROSITE PS50004, C2, 1 hit PS51210, PLA2C, 1 hit |
i Sequence
Sequence statusi: Complete.
Q50L41-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPWTLQPKWL AGKGLPLLGA ILLRKTEKSE PQWKHRRQET HPYYDLQVKV
60 70 80 90 100
LRARNIQHTD KLSKADCYVR LWLPTASVSP SQTRTVVNSS DPEWNETFPY
110 120 130 140 150
QIHGAVKNVL ELALYDEDVL DSDNVFSILF DTSTLQLGQP CTKNFTRQQD
160 170 180 190 200
PKELEVEFTL EKSQTPASEV VTNGVLVAHP CLRIQGTVTG DKTASLGELG
210 220 230 240 250
SRQIQLAVPG AYEKPQPLQP TSEPGLPVNF TFHVNPVLSP KLHIKLQEQL
260 270 280 290 300
QVFHSGPSDE LEAQTSKMDK ASILLSSLPL NEELTKLVDL EEGQQVSLRM
310 320 330 340 350
KADMSSSGDL DLRLGFDLCD GEQEFLDKRK QVASKALQRV MGLSEALHCD
360 370 380 390 400
QVPVVAVLGS GGGTRAMTSL YGSLAGLQEL GLLDAVTYLS GVSGSSWCIS
410 420 430 440 450
TLYRDPSWSQ KALQGPIKYA SERVCSSKIG MLSPKQFEYY SREKRAWESR
460 470 480 490 500
GHSMSFTDLW GLIIEYFLNQ EENPAKLSDQ QETVSQGQNP YPIYASINVH
510 520 530 540 550
KNISGDDFAE WCEFTPYEVG FPKYGAYVPT ELFGSEFFMG RLLHFWPEPR
560 570 580 590 600
ICYLQGMWGS AFAASLYEIF LKLGGLSLSF LDWHRGSVSV TDDWPKLRKQ
610 620 630 640 650
DPTRLPTRLF TPMSSFSQAV LDIFTSRITC AQTFNFTRGL CMYKDYTARK
660 670 680 690 700
DFVVSEDAWH SHNYGYPDAC PNQLTPMKDF LSLVDGGFAI NSPFPLVLQP
710 720 730 740 750
QRAVDLIVSF DYSLEGPFEV LQVTEKYCRD RGIPFPRIEV DPKDSEDPRE
760 770 780 790 800
CYLFAEAEDP CSPIVLHFPL VNRTFRTHLA PGVERQTAEE KAFGDFIING
810 820 830 840 850
PDTAYGMMDF TYEPKEFDRL VTLSRYNVLN NKETIRHALQ LALDRRRQAG
GRVGG
Sequence cautioni
The sequence AAH39947 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 99 | P → H in AAH39947 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 132 | T → M in AAH39947 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 234 | V → M in AAH39947 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 297 | S → T in AAH39947 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 507 | D → Y in AAH39947 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 507 | D → Y in AAH46400 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 526 | A → V in AAH39947 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 526 | A → V in AAH46400 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 755 | A → T in AAH39947 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 755 | A → T in AAH46400 (PubMed:15489334).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB195278 mRNA Translation: BAD98154.1 AL844608 Genomic DNA No translation available. BC039947 mRNA Translation: AAH39947.1 Different initiation. BC046400 mRNA Translation: AAH46400.1 |
CCDSi | CCDS16618.1 |
RefSeqi | NP_001019316.1, NM_001024145.2 |
Genome annotation databases
Ensembli | ENSMUST00000054651; ENSMUSP00000062607; ENSMUSG00000046971 |
GeneIDi | 271844 |
KEGGi | mmu:271844 |
UCSCi | uc008lvi.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB195278 mRNA Translation: BAD98154.1 AL844608 Genomic DNA No translation available. BC039947 mRNA Translation: AAH39947.1 Different initiation. BC046400 mRNA Translation: AAH46400.1 |
CCDSi | CCDS16618.1 |
RefSeqi | NP_001019316.1, NM_001024145.2 |
3D structure databases
SMRi | Q50L41 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000062607 |
Chemistry databases
SwissLipidsi | SLP:000001085 |
PTM databases
PhosphoSitePlusi | Q50L41 |
Proteomic databases
MaxQBi | Q50L41 |
PaxDbi | Q50L41 |
PRIDEi | Q50L41 |
ProteomicsDBi | 294373 |
Protocols and materials databases
Antibodypediai | 42117, 61 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000054651; ENSMUSP00000062607; ENSMUSG00000046971 |
GeneIDi | 271844 |
KEGGi | mmu:271844 |
UCSCi | uc008lvi.2, mouse |
Organism-specific databases
CTDi | 255189 |
MGIi | MGI:2685493, Pla2g4f |
Phylogenomic databases
eggNOGi | KOG1028, Eukaryota KOG1325, Eukaryota |
GeneTreei | ENSGT01000000214502 |
HOGENOMi | CLU_011663_0_0_1 |
InParanoidi | Q50L41 |
OMAi | GDFAEWC |
OrthoDBi | 302848at2759 |
TreeFami | TF325228 |
Enzyme and pathway databases
Reactomei | R-MMU-1482788, Acyl chain remodelling of PC R-MMU-1482801, Acyl chain remodelling of PS R-MMU-1482839, Acyl chain remodelling of PE R-MMU-1482922, Acyl chain remodelling of PI R-MMU-1482925, Acyl chain remodelling of PG R-MMU-1483115, Hydrolysis of LPC |
Miscellaneous databases
BioGRID-ORCSi | 271844, 1 hit in 52 CRISPR screens |
PROi | PR:Q50L41 |
RNActi | Q50L41, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000046971, Expressed in skeletal muscle tissue and 133 other tissues |
Genevisiblei | Q50L41, MM |
Family and domain databases
CDDi | cd04036, C2_cPLA2, 1 hit |
Gene3Di | 2.60.40.150, 1 hit |
InterProi | View protein in InterPro IPR016035, Acyl_Trfase/lysoPLipase IPR041847, C2_cPLA2 IPR000008, C2_dom IPR035892, C2_domain_sf IPR040723, cPLA2_C2 IPR002642, LysoPLipase_cat_dom |
Pfami | View protein in Pfam PF00168, C2, 1 hit PF18695, cPLA2_C2, 1 hit PF01735, PLA2_B, 1 hit |
SMARTi | View protein in SMART SM00239, C2, 1 hit SM00022, PLAc, 1 hit |
SUPFAMi | SSF52151, SSF52151, 1 hit |
PROSITEi | View protein in PROSITE PS50004, C2, 1 hit PS51210, PLA2C, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PA24F_MOUSE | |
Accessioni | Q50L41Primary (citable) accession number: Q50L41 Secondary accession number(s): A2AQJ2, Q80VQ8, Q80VV9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 25, 2006 |
Last sequence update: | July 27, 2011 | |
Last modified: | April 7, 2021 | |
This is version 116 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot