UniProtKB - Q50EK2 (TPSD1_PINTA)
Bifunctional levopimaradiene synthase, chloroplastic
LPS
Functioni
Involved in defensive oleoresin formation in conifers in response to insect attack or other injury. Involved in diterpene (C20) olefins biosynthesis. Bifunctional enzyme that catalyzes two sequential cyclizations of geranylgeranyl diphosphate (GGPP) to levopimaradiene. Levopimaradiene is the major products of the enzyme followed by abietadiene, neoabietadiene and palustradiene. No activity with geranyl diphosphate (GPP) or farnesyl diphosphate (FPP) as substrate.
1 PublicationCatalytic activityi
Cofactori
: oleoresin biosynthesis Pathwayi
This protein is involved in the pathway oleoresin biosynthesis, which is part of Terpene metabolism.View all proteins of this organism that are known to be involved in the pathway oleoresin biosynthesis and in Terpene metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 250 | SubstrateBy similarity | 1 | |
Metal bindingi | 383 | Magnesium 4By similarity | 1 | |
Metal bindingi | 385 | Magnesium 4By similarity | 1 | |
Binding sitei | 470 | SubstrateBy similarity | 1 | |
Metal bindingi | 602 | Magnesium 1By similarity | 1 | |
Metal bindingi | 602 | Magnesium 2By similarity | 1 | |
Metal bindingi | 606 | Magnesium 1By similarity | 1 | |
Metal bindingi | 606 | Magnesium 2By similarity | 1 | |
Metal bindingi | 746 | Magnesium 3By similarity | 1 | |
Metal bindingi | 750 | Magnesium 3By similarity | 1 | |
Metal bindingi | 754 | Magnesium 3By similarity | 1 |
GO - Molecular functioni
- copalyl diphosphate synthase activity Source: UniProtKB-EC
- levopimaradiene synthase activity Source: UniProtKB-EC
- magnesium ion binding Source: InterPro
- terpene synthase activity Source: InterPro
GO - Biological processi
- diterpenoid biosynthetic process Source: InterPro
Keywordsi
Molecular function | Isomerase, Lyase, Multifunctional enzyme |
Ligand | Magnesium, Metal-binding |
Enzyme and pathway databases
BRENDAi | 4.2.3.18, 4861 4.2.3.32, 4861 |
UniPathwayi | UPA00924 |
Names & Taxonomyi
Protein namesi | Recommended name: Bifunctional levopimaradiene synthase, chloroplasticShort name: PtLAS Alternative name(s): Diterpene synthase PtTPS-LAS Including the following 2 domains: |
Gene namesi | Name:LPS |
Organismi | Pinus taeda (Loblolly pine) |
Taxonomic identifieri | 3352 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Pinopsida › Pinidae › Conifers I › Pinaceae › Pinus › Pinus subgen. Pinus |
Subcellular locationi
Chloroplast
- chloroplast 1 Publication
Chloroplast
- chloroplast Source: UniProtKB-SubCell
Keywords - Cellular componenti
Chloroplast, PlastidPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 52 | ChloroplastSequence analysisAdd BLAST | 52 | |
ChainiPRO_0000348951 | 53 – 850 | Bifunctional levopimaradiene synthase, chloroplasticAdd BLAST | 798 |
Expressioni
Tissue specificityi
Inductioni
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 383 – 386 | DXDD motifCurated | 4 | |
Motifi | 602 – 606 | DDXXD motifCurated | 5 |
Domaini
Sequence similaritiesi
Keywords - Domaini
Transit peptidePhylogenomic databases
OMAi | HANTQCI |
Family and domain databases
CDDi | cd00684, Terpene_cyclase_plant_C1, 1 hit |
Gene3Di | 1.10.600.10, 1 hit 1.50.10.130, 1 hit |
InterProi | View protein in InterPro IPR008949, Isoprenoid_synthase_dom_sf IPR034741, Terpene_cyclase-like_1_C IPR044814, Terpene_cyclase_plant_C1 IPR001906, Terpene_synth_N IPR036965, Terpene_synth_N_sf IPR005630, Terpene_synthase_metal-bd IPR008930, Terpenoid_cyclase/PrenylTrfase |
Pfami | View protein in Pfam PF01397, Terpene_synth, 1 hit PF03936, Terpene_synth_C, 1 hit |
SFLDi | SFLDG01019, Terpene_Cyclase_Like_1_C_Termi, 1 hit |
SUPFAMi | SSF48239, SSF48239, 2 hits SSF48576, SSF48576, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MALPSSSLSS QIHTGATTQC IPHFHGSLNA GTSAGKRRSL YLRWGKGPSK
60 70 80 90 100
IVACAGQDPF SVPTLVKREF PPGFWKDHVI ESLMPSYKVA PSDEKRIETL
110 120 130 140 150
ITEIKNMFRS MGYGETNPSA YDTAWVARIP AVDGSEKPQF PETLEWILQN
160 170 180 190 200
QLKDGSWGEE FYFLAYDRIL ATLACIITLT IWQTGDTQVQ KGIEFFKTQA
210 220 230 240 250
GKIEEEADSH RPSGFEIVFP AMLKEAKALG LALPYELPFI QQIIEKREAK
260 270 280 290 300
LQRLPPDLLY ALPTTLLYSL EGLQEIVDWE KIMKLQSKDG SFLSSPASTA
310 320 330 340 350
AVFMRTGNKK CLEFLNFVLK KFGNHVPCHY PLDLFERLWA VDTVERLGID
360 370 380 390 400
HHFKEEIKDA LDYVYSHWDE RGIGWARENP VPDIDDTAMG LRILRLHGYN
410 420 430 440 450
VSSDVLKTFR DENGEFFCFL GQTQRGVTDM LNVNRCSHVA FPGETIMEEA
460 470 480 490 500
KLCTERYLRN ALEDGGASDK WALKKNIRGE VEYALKYPWH RSMPRLEARS
510 520 530 540 550
YIENYGPNDV WLGKTMYMMP NISNEKYLEL AKLDFNRVQF FHRQELQDIR
560 570 580 590 600
RWWNSSGFSQ LGFTRERVAE IYFSPASFLF EPEFATCRAV YTKTSNFTVI
610 620 630 640 650
LDDLYDAHGT LDNLKLFSES VKRWDLSLVD QMPQDMKICF KGFYNTFNEI
660 670 680 690 700
AEEGRKRQGR DVLSYIQKVW EVQLEAYTKE AEWSAVRYVP SYDEYIGNAS
710 720 730 740 750
VSIALGTVVL ISALFTGEIL TDDILSKIGR DSRFLYLMGL TGRLVNDTKT
760 770 780 790 800
YQAERGQGEV ASAVQCYMKD HPEISEEEAL KHVYTIMDNA LDELNREFVN
810 820 830 840 850
NRDVPDTCRR LVFETARIMQ LFYMDGDGLT LSHNMEIKEH VKNCLFQPVA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY779541 mRNA Translation: AAX07435.1 |
Genome annotation databases
KEGGi | ag:AAX07435 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY779541 mRNA Translation: AAX07435.1 |
3D structure databases
SMRi | Q50EK2 |
ModBasei | Search... |
Genome annotation databases
KEGGi | ag:AAX07435 |
Phylogenomic databases
OMAi | HANTQCI |
Enzyme and pathway databases
UniPathwayi | UPA00924 |
BRENDAi | 4.2.3.18, 4861 4.2.3.32, 4861 |
Family and domain databases
CDDi | cd00684, Terpene_cyclase_plant_C1, 1 hit |
Gene3Di | 1.10.600.10, 1 hit 1.50.10.130, 1 hit |
InterProi | View protein in InterPro IPR008949, Isoprenoid_synthase_dom_sf IPR034741, Terpene_cyclase-like_1_C IPR044814, Terpene_cyclase_plant_C1 IPR001906, Terpene_synth_N IPR036965, Terpene_synth_N_sf IPR005630, Terpene_synthase_metal-bd IPR008930, Terpenoid_cyclase/PrenylTrfase |
Pfami | View protein in Pfam PF01397, Terpene_synth, 1 hit PF03936, Terpene_synth_C, 1 hit |
SFLDi | SFLDG01019, Terpene_Cyclase_Like_1_C_Termi, 1 hit |
SUPFAMi | SSF48239, SSF48239, 2 hits SSF48576, SSF48576, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | TPSD1_PINTA | |
Accessioni | Q50EK2Primary (citable) accession number: Q50EK2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 2, 2008 |
Last sequence update: | June 7, 2005 | |
Last modified: | February 23, 2022 | |
This is version 70 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Documents
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families