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Protein

Adhesion G-protein coupled receptor G1

Gene

ADGRG1

Organism
Macaca mulatta (Rhesus macaque)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Receptor involved in cell adhesion and probably in cell-cell interactions. Mediates cell matrix adhesion in developing neurons and hematopoietic stem cells. Receptor for collagen III/COL3A1 in the developing brain and involved in regulation of cortical development, specifically in maintenance of the pial basement membrane integrity and in cortical lamination. Binding to the COL3A1 ligand inhibits neuronal migration and activates the RhoA pathway by coupling to GNA13 and possibly GNA12. Plays a role in the maintenance of hematopoietic stem cells and/or leukemia stem cells in bone marrow niche (By similarity). Plays an essential role in testis development (By similarity). Plays a critical role in tumourigenesis.By similarity

Enzyme regulationi

ADGRG1 NT is proposed to inhibit receptor signaling; its interactions with extracellular ligands and /or homophilic ADGRG1 NT interactions may relieve the inhibition. Following ligand binding to the N-terminal fragment, the N-terminal fragment is released from the seven-transmembrane C-terminal fragment to unveil a new N-terminal stalk, which then stimulates G-protein-dependent signaling activity. The N-terminal stalk has also been shown to be dispensable for at least some G-protein-dependent signaling.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein, G-protein coupled receptor, Heparin-binding, Receptor, Transducer
Biological processCell adhesion, Differentiation, Neurogenesis

Protein family/group databases

MEROPSiP02.008

Names & Taxonomyi

Protein namesi
Recommended name:
Adhesion G-protein coupled receptor G1
Alternative name(s):
G-protein coupled receptor 56
Cleaved into the following 2 chains:
ADGRG1 N-terminal fragment
Short name:
ADGRG1 NT
Alternative name(s):
GPR56 N-terminal fragment
Short name:
GPR56 NT
Short name:
GPR56(N)
GPR56 extracellular subunit
GPR56 subunit alpha
ADGRG1 C-terminal fragment
Short name:
ADGRG1 CT
Alternative name(s):
GPR56 C-terminal fragment
Short name:
GPR56 CT
Short name:
GPR56(C)
GPR56 seven-transmembrane subunit
Short name:
GPR56 7TM
GPR56 subunit beta
Gene namesi
Name:ADGRG1
Synonyms:GPR56
OrganismiMacaca mulatta (Rhesus macaque)
Taxonomic identifieri9544 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniCercopithecidaeCercopithecinaeMacaca
Proteomesi
  • UP000006718 Componenti: Chromosome 20

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini26 – 402ExtracellularSequence analysisAdd BLAST377
Transmembranei403 – 423Helical; Name=1Sequence analysisAdd BLAST21
Topological domaini424 – 442CytoplasmicSequence analysisAdd BLAST19
Transmembranei443 – 463Helical; Name=2Sequence analysisAdd BLAST21
Topological domaini464 – 470ExtracellularSequence analysis7
Transmembranei471 – 491Helical; Name=3Sequence analysisAdd BLAST21
Topological domaini492 – 512CytoplasmicSequence analysisAdd BLAST21
Transmembranei513 – 533Helical; Name=4Sequence analysisAdd BLAST21
Topological domaini534 – 570ExtracellularSequence analysisAdd BLAST37
Transmembranei571 – 591Helical; Name=5Sequence analysisAdd BLAST21
Topological domaini592 – 603CytoplasmicSequence analysisAdd BLAST12
Transmembranei604 – 624Helical; Name=6Sequence analysisAdd BLAST21
Topological domaini625 – 630ExtracellularSequence analysis6
Transmembranei631 – 651Helical; Name=7Sequence analysisAdd BLAST21
Topological domaini652 – 687CytoplasmicSequence analysisAdd BLAST36

Keywords - Cellular componenti

Cell membrane, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 25By similarityAdd BLAST25
ChainiPRO_000001288126 – 687Adhesion G-protein coupled receptor G1Add BLAST662
ChainiPRO_000042308826 – ?382ADGRG1 N-terminal fragmentBy similarityAdd BLAST357
ChainiPRO_0000423089?383 – 687ADGRG1 C-terminal fragmentBy similarityAdd BLAST305

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi39N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi148N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi156N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi171N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi234N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi303N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi324N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi341N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

Autoproteolytically cleaved into 2 fragments; the large extracellular N-terminal fragment and the membrane-bound C-terminal fragment predominantly remain associated and non-covalently linked. Shedding to yield the secreted ADGRG1 N-terminal fragment seems to involve metalloprotease(s).By similarity
N-glycosylated. Contains sialic acid residues.By similarity
Ubiquitinated. Undergoes polyubiquitination upon activation.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei382 – 383Cleavage; by autolysisBy similarity2

Keywords - PTMi

Glycoprotein, Ubl conjugation

Expressioni

Gene expression databases

ExpressionAtlasiQ50DM8 baseline

Interactioni

Subunit structurei

Heterodimer of 2 chains generated by proteolytic processing; the large extracellular N-terminal fragment (ADGRG1 NT) and the membrane-bound C-terminal fragment (ADGRG1-CT) predominantly remain associated and non-covalently linked. ADGRG1 NT self-associates in a trans-trans manner; the homophilic interaction enhances receptor signaling. ADGRG1-CT interacts with ARRB2; the interaction is impaired by ADGRG1 NT. Interacts with TGM2; TGM2 probably is not a ADGRG1 ligand and the interaction is reported controversial. Part of a GPCR-tetraspanin complex at least consisting of ADGRG1, CD81, eventually CD9, and GNA11 in which CD81 is enhancing the association of ADGRG1 with GNA11. Interacts with heparin; leading to the reduction of ADGRG1 shedding.By similarity

Protein-protein interaction databases

STRINGi9544.ENSMMUP00000020590

Structurei

3D structure databases

ProteinModelPortaliQ50DM8
SMRiQ50DM8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini343 – 394GPSPROSITE-ProRule annotationAdd BLAST52

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni26 – 33Heparin-bindingBy similarity8
Regioni190 – 200Heparin-bindingBy similarityAdd BLAST11

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4193 Eukaryota
ENOG410XSD2 LUCA
GeneTreeiENSGT00920000148984
HOGENOMiHOG000015136
HOVERGENiHBG051814
InParanoidiQ50DM8
KOiK08450

Family and domain databases

InterProiView protein in InterPro
IPR017981 GPCR_2-like
IPR000832 GPCR_2_secretin-like
IPR003910 GPR1/GPR3/GPR5
IPR000203 GPS
PfamiView protein in Pfam
PF00002 7tm_2, 1 hit
PF01825 GPS, 1 hit
PRINTSiPR00249 GPCRSECRETIN
PR01422 GPR56ORPHANR
SMARTiView protein in SMART
SM00303 GPS, 1 hit
PROSITEiView protein in PROSITE
PS50261 G_PROTEIN_RECEP_F2_4, 1 hit
PS50221 GPS, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q50DM8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTAQSLLQTT LFLLSLLFLV QGAHGRGHRE DFRFCSQRNQ THISSLHYKF
60 70 80 90 100
TPDLRISIEN SEEALTVHAP FPEAHPASRS FPHPRGLYHF CLYWDRHAGR
110 120 130 140 150
LHLLYGKHDF LLSDQASSLL CFQHQEESLA QGPPLFATSV TSWWSPQNIS
160 170 180 190 200
LPSASNFTFS FHSPPHTAAH NASVDMCELK RDLQLLSQFL KHPQKASRRP
210 220 230 240 250
SATPASQQLQ SLESKLTSVR FMGDTVSFEE DRVNATVWKL QPTAGLQDLH
260 270 280 290 300
IHSRQEQEQS EILEYSVLLP RTLFQRTKGR RGEAEKRLLL VDFSSQALFQ
310 320 330 340 350
DKNSSQVLGE KVLGIVVQNT KVANLTEPVV LTFQHQPQPK NVTLQCVFWV
360 370 380 390 400
EDPTLSNPGR WSSAGCETVR RETQTSCFCN HLTYFAVLMV SSVEVDAVHK
410 420 430 440 450
HYLSLLSYVG CVVSALACVV TIAAYLCSRR KPRDYTIKVH MNLLLAVFLL
460 470 480 490 500
DVSFLLSEPV ALTGSQSGCR ASAIFLHFSL LACLSWMGLE GYNLYRLVVE
510 520 530 540 550
VFGTYIPGYL LKLSAMGWGF PIFLVTLVAL VDVDNYGPII LAVHRTPESV
560 570 580 590 600
IYPSMCWIRD SLVSYITNLG LFSLVFLFNM AMLGTMVVQI LRLRPHTQKW
610 620 630 640 650
SHVLTLLGLS LVLGLPWALI FFSFASGTFQ LVVLYLFSII TSFQGFLIFL
660 670 680
WYWSMRLQAR GGPSPLKSNS DSARLPISTG STSSSRI
Length:687
Mass (Da):77,271
Last modified:June 7, 2005 - v1
Checksum:iCFE8C6F9C81B91DF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY845352 mRNA Translation: AAX56343.1
RefSeqiNP_001028192.1, NM_001033020.1
XP_014981658.1, XM_015126172.1
UniGeneiMmu.10085

Genome annotation databases

EnsembliENSMMUT00000039308; ENSMMUP00000032394; ENSMMUG00000015689
GeneIDi613233
KEGGimcc:613233

Similar proteinsi

Entry informationi

Entry nameiAGRG1_MACMU
AccessioniPrimary (citable) accession number: Q50DM8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: June 7, 2005
Last modified: June 20, 2018
This is version 84 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families

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