Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Hydroxylamine oxidoreductase

Gene

hao1

more
Organism
Nitrosomonas europaea (strain ATCC 19718 / CIP 103999 / KCTC 2705 / NBRC 14298)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Catalyzes the oxidation of hydroxylamine to nitrite. The electrons released in the reaction are partitioned to ammonium monooxygenase and to the respiratory chain. The immediate acceptor of electrons from HAO is cytochrome c-554.1 Publication

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the ‘Function’ section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

heme c1 PublicationNote: Binds 8 heme c groups per subunit. One specific heme c group is called heme P460.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Function’ section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei103Heme 1 (covalent)1 Publication1
Binding sitei106Heme 1 (covalent)1 Publication1
<p>This subsection of the ‘Function’ section indicates at which position the protein binds a given metal ion. The nature of the metal is indicated in the ‘Description’ field.<p><a href='/help/metal' target='_top'>More...</a></p>Metal bindingi107Iron (heme 1 axial ligand)1
Metal bindingi123Iron (heme 3 axial ligand)1
Binding sitei169Heme 2 (covalent)1 Publication1
Binding sitei172Heme 2 (covalent)1 Publication1
Metal bindingi173Iron (heme 2 axial ligand)1
Metal bindingi184Iron (heme 1 axial ligand)1
Binding sitei196Heme 3 (covalent)1 Publication1
Binding sitei199Heme 3 (covalent)1 Publication1
Metal bindingi200Iron (heme 3 axial ligand)1
Metal bindingi228Iron (heme 6 axial ligand)1
Binding sitei253Heme 4 (covalent; via 3 links); shared with Y-491 in trimeric partner 11
Binding sitei256Heme 4 (covalent; via 3 links); shared with Y-491 in trimeric partner 11
Metal bindingi257Iron (heme 4 axial ligand)1
Binding sitei263Heme 5 (covalent)1 Publication1
Binding sitei266Heme 5 (covalent)1 Publication1
Metal bindingi267Iron (heme 5 axial ligand)1
Metal bindingi270Iron (heme 2 axial ligand)1
Binding sitei283Heme 6 (covalent)1
Binding sitei286Heme 6 (covalent)1 Publication1
Metal bindingi287Iron (heme 6 axial ligand)1
Metal bindingi303Iron (heme 8 axial ligand)1
Binding sitei334Heme 7 (covalent)1 Publication1
Binding sitei337Heme 7 (covalent)1 Publication1
Metal bindingi338Iron (heme 7 axial ligand)1
Metal bindingi347Iron (heme 5 axial ligand)1
Binding sitei384Heme 8 (covalent)1 Publication1
Binding sitei387Heme 8 (covalent)1 Publication1
Metal bindingi388Iron (heme 8 axial ligand)1
Metal bindingi483Iron (heme 7 axial ligand)1
Binding sitei491Heme 4 (covalent; via 3 links); shared with C-253 and C-256 in trimeric partner 21

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

  • hydroxylamine oxidase activity Source: CACAO
  • metal ion binding Source: UniProtKB-KW
  • oxidoreductase activity Source: CACAO

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionOxidoreductase
LigandHeme, Iron, Metal-binding

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...
BioCyci
MetaCyc:HAONITRO-MONOMER

BRENDA Comprehensive Enzyme Information System

More...
BRENDAi
1.7.2.6 3654

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Hydroxylamine oxidoreductase (EC:1.7.2.61 Publication)
Short name:
HAO
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:hao1
Ordered Locus Names:NE2044
AND
Name:hao2
Ordered Locus Names:NE0962
AND
Name:hao3
Ordered Locus Names:NE2339
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiNitrosomonas europaea (strain ATCC 19718 / CIP 103999 / KCTC 2705 / NBRC 14298)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri228410 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNitrosomonadalesNitrosomonadaceaeNitrosomonas
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000001416 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Periplasm

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section denotes the presence of an N-terminal signal peptide.<p><a href='/help/signal' target='_top'>More...</a></p>Signal peptidei1 – 24Add BLAST24
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_000000660425 – 570Hydroxylamine oxidoreductase1 PublicationAdd BLAST546

<p>This subsection of the <a href="http://www.uniprot.org/help/ptm_processing_section">PTM/processing</a> section describes post-translational modifications (PTMs). This subsection <strong>complements</strong> the information provided at the sequence level or describes modifications for which <strong>position-specific data is not yet available</strong>.<p><a href='/help/post-translational_modification' target='_top'>More...</a></p>Post-translational modificationi

Binds 8 heme groups per subunit.

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Homotrimer.

Protein-protein interaction databases

STRING: functional protein association networks

More...
STRINGi
228410.NE2339

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

1570
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

Protein Model Portal of the PSI-Nature Structural Biology Knowledgebase

More...
ProteinModelPortali
Q50925

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
Q50925

Database of comparative protein structure models

More...
ModBasei
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

Miscellaneous databases

Relative evolutionary importance of amino acids within a protein sequence

More...
EvolutionaryTracei
Q50925

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Keywords - Domaini

Signal

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
ENOG410690C Bacteria
ENOG410XPVG LUCA

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
HOG000064068

KEGG Orthology (KO)

More...
KOi
K10535

Identification of Orthologs from Complete Genome Data

More...
OMAi
PQWEMWS

Database of Orthologous Groups

More...
OrthoDBi
POG091H0RC8

Family and domain databases

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR012138 HAO
IPR011031 Multihaem_cyt
IPR036280 Multihaem_cyt_sf

PIRSF; a whole-protein classification database

More...
PIRSFi
PIRSF000242 HAO, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF48695 SSF48695, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS51008 MULTIHEME_CYTC, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>.<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is in its mature form or if it represents the precursor.<p><a href='/help/sequence_processing' target='_top'>More...</a></p>Sequence processingi: The displayed sequence is further processed into a mature form.

Q50925-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MRIGEWMRGL LLCAGLMMCG VVHADISTVP DETYDALKLD RGKATPKETY
60 70 80 90 100
EALVKRYKDP AHGAGKGTMG DYWEPIAISI YMDPNTFYKP PVSPKEVAER
110 120 130 140 150
KDCVECHSDE TPVWVRAWKR STHANLDKIR NLKSDDPLYY KKGKLEEVEN
160 170 180 190 200
NLRSMGKLGE KETLKEVGCI DCHVDVNKKD KADHTKDIRM PTADTCGTCH
210 220 230 240 250
LREFAERESE RDTMVWPNGQ WPAGRPSHAL DYTANIETTV WAAMPQREVA
260 270 280 290 300
EGCTMCHTNQ NKCDNCHTRH EFSAAESRKP EACATCHSGV DHNNWEAYTM
310 320 330 340 350
SKHGKLAEMN RDKWNWEVRL KDAFSKGGQN APTCAACHME YEGEYTHNIT
360 370 380 390 400
RKTRWANYPF VPGIAENITS DWSEARLDSW VLTCTQCHSE RFARSYLDLM
410 420 430 440 450
DKGTLEGLAK YQEANAIVHK MYEDGTLTGQ KTNRPNPPEP EKPGFGIFTQ
460 470 480 490 500
LFWSKGNNPA SLELKVLEMA ENNLAKMHVG LAHVNPGGWT YTEGWGPMNR
510 520 530 540 550
AYVEIQDEYT KMQELSALQA RVNKLEGKQT SLLDLKGTGE KISLGGLGGG
560 570
MLLAGALALI GWRKRKQTRA
Length:570
Mass (Da):64,259
Last modified:May 16, 2003 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iC76AB9019512105E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Sequence’ section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti243A → T in AAC43216 (PubMed:8288544).Curated1
Sequence conflicti470A → G in AAC43216 (PubMed:8288544).Curated1

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
U04053 Unassigned DNA Translation: AAC43216.1
AL954747 Genomic DNA Translation: CAD84873.1
AL954747 Genomic DNA Translation: CAD85955.1
AL954747 Genomic DNA Translation: CAD86251.1

Protein sequence database of the Protein Information Resource

More...
PIRi
A36954

NCBI Reference Sequences

More...
RefSeqi
WP_011111571.1, NC_004757.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...
EnsemblBacteriai
CAD84873; CAD84873; NE0962
CAD85955; CAD85955; NE2044
CAD86251; CAD86251; NE2339

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
neu:NE0962
neu:NE2044
neu:NE2339

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U04053 Unassigned DNA Translation: AAC43216.1
AL954747 Genomic DNA Translation: CAD84873.1
AL954747 Genomic DNA Translation: CAD85955.1
AL954747 Genomic DNA Translation: CAD86251.1
PIRiA36954
RefSeqiWP_011111571.1, NC_004757.1

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...
PDBei

Protein Data Bank RCSB

More...
RCSB PDBi

Protein Data Bank Japan

More...
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1FGJX-ray2.80A/B25-570[»]
4FASX-ray2.10A/B/C25-570[»]
4N4NX-ray2.20A/C/E25-570[»]
4N4OX-ray2.47A/C/E25-570[»]
ProteinModelPortaliQ50925
SMRiQ50925
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi228410.NE2339

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAD84873; CAD84873; NE0962
CAD85955; CAD85955; NE2044
CAD86251; CAD86251; NE2339
KEGGineu:NE0962
neu:NE2044
neu:NE2339

Phylogenomic databases

eggNOGiENOG410690C Bacteria
ENOG410XPVG LUCA
HOGENOMiHOG000064068
KOiK10535
OMAiPQWEMWS
OrthoDBiPOG091H0RC8

Enzyme and pathway databases

BioCyciMetaCyc:HAONITRO-MONOMER
BRENDAi1.7.2.6 3654

Miscellaneous databases

EvolutionaryTraceiQ50925

Family and domain databases

InterProiView protein in InterPro
IPR012138 HAO
IPR011031 Multihaem_cyt
IPR036280 Multihaem_cyt_sf
PIRSFiPIRSF000242 HAO, 1 hit
SUPFAMiSSF48695 SSF48695, 1 hit
PROSITEiView protein in PROSITE
PS51008 MULTIHEME_CYTC, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiHAO_NITEU
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q50925
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: May 16, 2003
Last modified: December 5, 2018
This is version 128 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again