UniProtKB - Q505F1 (NR2C1_MOUSE)
Nuclear receptor subfamily 2 group C member 1
Nr2c1
Functioni
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 98 – 173 | Nuclear receptorPROSITE-ProRule annotationAdd BLAST | 76 | |
Zinc fingeri | 101 – 121 | NR C4-typePROSITE-ProRule annotationAdd BLAST | 21 | |
Zinc fingeri | 137 – 156 | NR C4-typePROSITE-ProRule annotationAdd BLAST | 20 |
GO - Molecular functioni
- DNA binding Source: UniProtKB
- DNA-binding transcription repressor activity, RNA polymerase II-specific Source: NTNU_SB
- histone deacetylase binding Source: UniProtKB
- nuclear receptor activity Source: GO_Central
- protein homodimerization activity Source: UniProtKB
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: NTNU_SB
- sequence-specific double-stranded DNA binding Source: MGI
- zinc ion binding Source: InterPro
GO - Biological processi
- anatomical structure development Source: GO_Central
- cell differentiation Source: GO_Central
- negative regulation of transcription, DNA-templated Source: UniProtKB
- negative regulation of transcription by RNA polymerase II Source: UniProtKB
- positive regulation of retinoic acid receptor signaling pathway Source: UniProtKB
- regulation of transcription by RNA polymerase II Source: GO_Central
Keywordsi
Molecular function | Activator, DNA-binding, Receptor, Repressor |
Biological process | Transcription, Transcription regulation |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-MMU-383280, Nuclear Receptor transcription pathway |
Names & Taxonomyi
Protein namesi | Recommended name: Nuclear receptor subfamily 2 group C member 1Alternative name(s): Orphan nuclear receptor TR2 Testicular receptor 2 Short name: mTR2 |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1352465, Nr2c1 |
Subcellular locationi
Nucleus
- nuclear chromatin Source: GO_Central
- nucleoplasm Source: Reactome
- nucleus Source: UniProtKB
- PML body Source: UniProtKB
Keywords - Cellular componenti
NucleusPathology & Biotechi
Disruption phenotypei
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 167 | K → A: No effect on sumoylation. 1 Publication | 1 | |
Mutagenesisi | 170 | S → A: No effect on sumoylation nor on DNA-binding and little effect on binding KAT2B. Greatly reduced DNA-binding, binding to KAT2B and activation of the RARB promoter; when associated with A-185. 2 Publications | 1 | |
Mutagenesisi | 185 | S → A: No effect on sumoylation and little effect on binding KAT2B. Some reduced DNA-binding. Greatly reduced DNA-binding, binding to KAT2B and activation of the RARB promoter; when associated with A-170. 2 Publications | 1 | |
Mutagenesisi | 203 | S → A: No effect on sumoylation. 1 Publication | 1 | |
Mutagenesisi | 208 | T → A: No effect on sumoylation. 1 Publication | 1 | |
Mutagenesisi | 210 | T → A: Abolishes sumoylation. No repression of OCT4 gene expression with or without retinoic acid and enhanced interaction with KAT2B and HDAC3. Abolishes interaction with HDAC3 and PML association; when associated with R-238. 1 Publication | 1 | |
Mutagenesisi | 210 | T → E: Sumoylated. Repressed OCT4 gene expression. Enhanced interaction with KAT2B; when associated with R-238. 1 Publication | 1 | |
Mutagenesisi | 238 | K → A: Abolishes sumoylation. Strongly associated with PML nuclear bodies. No effect on activation of OCT4 but activation not suppressed by additional SUMO1. Increased binding to KAT2B and reduced binding to NRIP1. Abolishes PML association; when associated with A-210. 2 Publications | 1 | |
Mutagenesisi | 238 | K → R: Abolishes sumoylation. No effect on activation of OCT4 but activation not suppressed by additional SUMO1. Enhanced interaction with KAT2B; when associated with A-210 or E-210. 2 Publications | 1 | |
Mutagenesisi | 240 | E → A: Abolishes sumoylation. 1 Publication | 1 | |
Mutagenesisi | 461 | S → A: Little effect on PKC-stimulated protein stability nor on activation of RARB reporter. 1 Publication | 1 | |
Mutagenesisi | 461 | S → A: No effect on sumoylation. 1 Publication | 1 | |
Mutagenesisi | 568 | S → A: Greatly reduced PKC-stimulated protein stability and activation of RARB reporter. 1 Publication | 1 | |
Mutagenesisi | 568 | S → A: No effect on sumoylation. 1 Publication | 1 | |
Mutagenesisi | 575 | K → R: No effect on sumoylation. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000053587 | 1 – 590 | Nuclear receptor subfamily 2 group C member 1Add BLAST | 590 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 185 | Phosphoserine1 Publication | 1 | |
Modified residuei | 203 | Phosphoserine1 Publication | 1 | |
Modified residuei | 208 | Phosphothreonine1 Publication | 1 | |
Modified residuei | 210 | Phosphothreonine; by MAPK11 Publication | 1 | |
Cross-linki | 238 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO); alternate1 Publication | ||
Cross-linki | 238 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity | ||
Modified residuei | 461 | Phosphoserine; by PKC1 Publication | 1 | |
Modified residuei | 568 | Phosphoserine; by PKC1 Publication | 1 | |
Cross-linki | 575 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity |
Post-translational modificationi
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q505F1 |
jPOSTi | Q505F1 |
PaxDbi | Q505F1 |
PeptideAtlasi | Q505F1 |
PRIDEi | Q505F1 |
PTM databases
iPTMneti | Q505F1 |
PhosphoSitePlusi | Q505F1 |
Expressioni
Tissue specificityi
Developmental stagei
Inductioni
Gene expression databases
Bgeei | ENSMUSG00000005897, Expressed in retina and 273 other tissues |
Genevisiblei | Q505F1, MM |
Interactioni
Subunit structurei
Homodimer. Heterodimer; with NR2C2 which is required for chromatin remodeling and for binding to promoter regions such as globin DR1 repeats.
Interacts with ESR1; the interaction prevents homodimerization of ESR1 and suppresses its transcriptional activity and cell growth (By similarity).
Interacts with NRIP1 (via its LXXLL motifs); the interaction provides corepressor activity.
Interacts with HDAC3 (via the DNA-binding domain); the interaction recruits phosphorylated NR2C1 to PML bodies for sumoylation.
Interacts with HDAC4 (via the DNA-binding domain).
Interacts with PIAS1; the interaction is required for sumoylation of NR2C1.
Interacts with UBE2I; the interaction is required for sumoylation of NR2C1.
Interacts with KAT2B; the interaction acts as a corepressor of gene expression.
By similarity6 PublicationsBinary interactionsi
Hide detailsQ505F1
GO - Molecular functioni
- histone deacetylase binding Source: UniProtKB
- protein homodimerization activity Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 204299, 2 interactors |
DIPi | DIP-29275N |
IntActi | Q505F1, 7 interactors |
STRINGi | 10090.ENSMUSP00000100927 |
Miscellaneous databases
RNActi | Q505F1, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 333 – 577 | NR LBDPROSITE-ProRule annotationAdd BLAST | 245 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 166 | Required for interaction with KAT2BAdd BLAST | 166 | |
Regioni | 571 – 590 | Required for interaction with NRIP1Add BLAST | 20 |
Sequence similaritiesi
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 101 – 121 | NR C4-typePROSITE-ProRule annotationAdd BLAST | 21 | |
Zinc fingeri | 137 – 156 | NR C4-typePROSITE-ProRule annotationAdd BLAST | 20 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | KOG3575, Eukaryota |
GeneTreei | ENSGT00940000158165 |
HOGENOMi | CLU_007368_16_2_1 |
InParanoidi | Q505F1 |
OMAi | LVMEHIF |
OrthoDBi | 278171at2759 |
TreeFami | TF316650 |
Family and domain databases
Gene3Di | 1.10.565.10, 1 hit 3.30.50.10, 1 hit |
InterProi | View protein in InterPro IPR035500, NHR-like_dom_sf IPR000536, Nucl_hrmn_rcpt_lig-bd IPR001723, Nuclear_hrmn_rcpt IPR001628, Znf_hrmn_rcpt IPR013088, Znf_NHR/GATA |
Pfami | View protein in Pfam PF00104, Hormone_recep, 1 hit PF00105, zf-C4, 1 hit |
PRINTSi | PR00398, STRDHORMONER PR00047, STROIDFINGER |
SMARTi | View protein in SMART SM00430, HOLI, 1 hit SM00399, ZnF_C4, 1 hit |
SUPFAMi | SSF48508, SSF48508, 1 hit |
PROSITEi | View protein in PROSITE PS51843, NR_LBD, 1 hit PS00031, NUCLEAR_REC_DBD_1, 1 hit PS51030, NUCLEAR_REC_DBD_2, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MATIEEIAHQ IIDQQMGEIV TEQQTGQKIQ IVTALDHSTQ GKQFILANHE
60 70 80 90 100
GSTPGKVFLT TPDAAGVNQL FFTSPDLSAP HLQLLTEKSP DQGPNKVFDL
110 120 130 140 150
CVVCGDKASG RHYGAITCEG CKGFFKRSIR KNLVYSCRGS KDCVINKHHR
160 170 180 190 200
NRCQYCRLQR CIAFGMKQDS VQCERKPIEV SREKSSNCAA STEKIYIRKD
210 220 230 240 250
LRSPLAATPT FVTDSETARS AGLLDSGMFV NIHPSGIKTE PAMLMAPDKA
260 270 280 290 300
ESCQGDLSTL ASVVTSLANL GKAKDLSHCG GDMPVVQSLR NGDTSFGAFH
310 320 330 340 350
HDIQTNGDVS RAFDTLAKAL TPGESSACQS PEEGMEGSPH LIAGEPSFVE
360 370 380 390 400
KEGPLLSESH VAFRLTMPSP MPEYLNVHYI GESASRLLFL SMHWALSIPS
410 420 430 440 450
FQALGQENSI SLVKAYWNEL FTLGLAQCWQ VMNVATILAT FVNCLHSSLQ
460 470 480 490 500
QDKMSPERRK SLMEHIFKLQ EFCNSMVKLC IDGHEYAYLK AIVLFSPDHP
510 520 530 540 550
GLENMELIER FQEKAYVEFQ DYITRTYPDD TYRLSRLLLR LPALRLMNAT
560 570 580 590
ITEELFFKGL IGNVRIDSVI PHILKMEPAD YNSQIIGHSL
The sequence of this isoform differs from the canonical sequence as follows:
220-256: SAGLLDSGMFVNIHPSGIKTEPAMLMAPDKAESCQGD → CPAAISASFASLPRSTETKTCASFVAGQLDCWIQECL
257-590: Missing.
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 88 | K → N in CAA72244 (PubMed:9504722).Curated | 1 | |
Sequence conflicti | 88 | K → N in AAC29502 (PubMed:9694834).Curated | 1 | |
Sequence conflicti | 88 | K → N in AAL31315 (Ref. 6) Curated | 1 | |
Sequence conflicti | 200 | D → N in AAC29502 (PubMed:9694834).Curated | 1 | |
Sequence conflicti | 200 | D → N in AAL31315 (Ref. 6) Curated | 1 | |
Sequence conflicti | 273 | A → T in AAC29502 (PubMed:9694834).Curated | 1 | |
Sequence conflicti | 273 | A → T in AAL31315 (Ref. 6) Curated | 1 | |
Sequence conflicti | 326 – 327 | SA → TS in AAC52787 (PubMed:8595902).Curated | 2 | |
Sequence conflicti | 326 – 327 | SA → TS in AAC53253 (PubMed:8595902).Curated | 2 | |
Sequence conflicti | 326 – 327 | SA → TS in CAA72244 (PubMed:9504722).Curated | 2 | |
Sequence conflicti | 326 – 327 | SA → TS in AAL31315 (Ref. 6) Curated | 2 | |
Sequence conflicti | 331 | P → S in AAC29502 (PubMed:9694834).Curated | 1 | |
Sequence conflicti | 331 | P → S in AAL31315 (Ref. 6) Curated | 1 | |
Sequence conflicti | 529 | D → N in BAE27509 (PubMed:16141072).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 29 | I → M in strain: CD-1. 2 Publications | 1 | |
Natural varianti | 145 | I → M in strain: CD-1. 2 Publications | 1 | |
Natural varianti | 296 – 304 | FGAFHHDIQ → SVLFIMIFK in strain: CD-1. 2 Publications | 9 | |
Natural varianti | 361 | V → I in strain: CD-1. 2 Publications | 1 | |
Natural varianti | 377 | V → A in strain: CD-1. 2 Publications | 1 | |
Natural varianti | 456 | P → A in strain: CD-1. 2 Publications | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_051921 | 220 – 256 | SAGLL…SCQGD → CPAAISASFASLPRSTETKT CASFVAGQLDCWIQECL in isoform 2. 1 PublicationAdd BLAST | 37 | |
Alternative sequenceiVSP_051922 | 257 – 590 | Missing in isoform 2. 1 PublicationAdd BLAST | 334 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U28280 , U28269, U28270, U28271, U28272, U28273, U28274, U28275, U28276, U28277, U28278, U28279 Genomic DNA Translation: AAC53253.1 U28265 mRNA Translation: AAC52787.1 Y11436 mRNA Translation: CAA72244.1 U30482 mRNA Translation: AAC29502.1 L26957 mRNA Translation: AAL31315.1 AK146891 mRNA Translation: BAE27509.1 AK149374 mRNA Translation: BAE28842.1 AC127596 Genomic DNA No translation available. AC138026 Genomic DNA No translation available. BC090662 mRNA Translation: AAH90662.1 BC094580 mRNA Translation: AAH94580.1 |
CCDSi | CCDS24131.1 [Q505F1-1] |
RefSeqi | NP_035759.3, NM_011629.3 [Q505F1-1] XP_006513655.1, XM_006513592.2 [Q505F1-1] |
Genome annotation databases
Ensembli | ENSMUST00000092213; ENSMUSP00000089858; ENSMUSG00000005897 [Q505F1-1] ENSMUST00000099343; ENSMUSP00000096945; ENSMUSG00000005897 [Q505F1-1] ENSMUST00000105290; ENSMUSP00000100927; ENSMUSG00000005897 [Q505F1-1] |
GeneIDi | 22025 |
KEGGi | mmu:22025 |
UCSCi | uc007gvo.2, mouse [Q505F1-1] |
Keywords - Coding sequence diversityi
Alternative splicing, PolymorphismSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U28280 , U28269, U28270, U28271, U28272, U28273, U28274, U28275, U28276, U28277, U28278, U28279 Genomic DNA Translation: AAC53253.1 U28265 mRNA Translation: AAC52787.1 Y11436 mRNA Translation: CAA72244.1 U30482 mRNA Translation: AAC29502.1 L26957 mRNA Translation: AAL31315.1 AK146891 mRNA Translation: BAE27509.1 AK149374 mRNA Translation: BAE28842.1 AC127596 Genomic DNA No translation available. AC138026 Genomic DNA No translation available. BC090662 mRNA Translation: AAH90662.1 BC094580 mRNA Translation: AAH94580.1 |
CCDSi | CCDS24131.1 [Q505F1-1] |
RefSeqi | NP_035759.3, NM_011629.3 [Q505F1-1] XP_006513655.1, XM_006513592.2 [Q505F1-1] |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 204299, 2 interactors |
DIPi | DIP-29275N |
IntActi | Q505F1, 7 interactors |
STRINGi | 10090.ENSMUSP00000100927 |
PTM databases
iPTMneti | Q505F1 |
PhosphoSitePlusi | Q505F1 |
Proteomic databases
EPDi | Q505F1 |
jPOSTi | Q505F1 |
PaxDbi | Q505F1 |
PeptideAtlasi | Q505F1 |
PRIDEi | Q505F1 |
Protocols and materials databases
Antibodypediai | 4166, 405 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000092213; ENSMUSP00000089858; ENSMUSG00000005897 [Q505F1-1] ENSMUST00000099343; ENSMUSP00000096945; ENSMUSG00000005897 [Q505F1-1] ENSMUST00000105290; ENSMUSP00000100927; ENSMUSG00000005897 [Q505F1-1] |
GeneIDi | 22025 |
KEGGi | mmu:22025 |
UCSCi | uc007gvo.2, mouse [Q505F1-1] |
Organism-specific databases
CTDi | 7181 |
MGIi | MGI:1352465, Nr2c1 |
Phylogenomic databases
eggNOGi | KOG3575, Eukaryota |
GeneTreei | ENSGT00940000158165 |
HOGENOMi | CLU_007368_16_2_1 |
InParanoidi | Q505F1 |
OMAi | LVMEHIF |
OrthoDBi | 278171at2759 |
TreeFami | TF316650 |
Enzyme and pathway databases
Reactomei | R-MMU-383280, Nuclear Receptor transcription pathway |
Miscellaneous databases
BioGRID-ORCSi | 22025, 0 hits in 17 CRISPR screens |
ChiTaRSi | Nr2c1, mouse |
PROi | PR:Q505F1 |
RNActi | Q505F1, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000005897, Expressed in retina and 273 other tissues |
Genevisiblei | Q505F1, MM |
Family and domain databases
Gene3Di | 1.10.565.10, 1 hit 3.30.50.10, 1 hit |
InterProi | View protein in InterPro IPR035500, NHR-like_dom_sf IPR000536, Nucl_hrmn_rcpt_lig-bd IPR001723, Nuclear_hrmn_rcpt IPR001628, Znf_hrmn_rcpt IPR013088, Znf_NHR/GATA |
Pfami | View protein in Pfam PF00104, Hormone_recep, 1 hit PF00105, zf-C4, 1 hit |
PRINTSi | PR00398, STRDHORMONER PR00047, STROIDFINGER |
SMARTi | View protein in SMART SM00430, HOLI, 1 hit SM00399, ZnF_C4, 1 hit |
SUPFAMi | SSF48508, SSF48508, 1 hit |
PROSITEi | View protein in PROSITE PS51843, NR_LBD, 1 hit PS00031, NUCLEAR_REC_DBD_1, 1 hit PS51030, NUCLEAR_REC_DBD_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NR2C1_MOUSE | |
Accessioni | Q505F1Primary (citable) accession number: Q505F1 Secondary accession number(s): P97763 Q8VIJ3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 6, 2005 |
Last sequence update: | July 27, 2011 | |
Last modified: | December 2, 2020 | |
This is version 140 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families