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Protein

ATPase get3

Gene

get3

Organism
Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound receptors, where the tail-anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis. ATP binding drives the homodimer towards the closed dimer state, facilitating recognition of newly synthesized TA membrane proteins. ATP hydrolysis is required for insertion. Subsequently, the homodimer reverts towards the open dimer state, lowering its affinity for the membrane-bound receptor, and returning it to the cytosol to initiate a new round of targeting.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei63UniRule annotation1
Binding sitei245ATPUniRule annotation1
Binding sitei272ATP1
Metal bindingi283Zinc; shared with dimeric partnerUniRule annotation1
Metal bindingi286Zinc; shared with dimeric partnerUniRule annotation1
Binding sitei320ATP1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi34 – 41ATP8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processTransport
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
ATPase get3UniRule annotation (EC:3.6.-.-UniRule annotation)
Alternative name(s):
Arsenical pump-driving ATPaseUniRule annotation
Arsenite-stimulated ATPaseUniRule annotation
Golgi to ER traffic protein 3UniRule annotation
Guided entry of tail-anchored proteins 3UniRule annotation
Gene namesi
Name:get3
ORF Names:AFUA_3G11350
OrganismiNeosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
Taxonomic identifieri330879 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000002530 Componentsi: Chromosome 3, Unassembled WGS sequence

Organism-specific databases

EuPathDBiFungiDB:Afu3g11350

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi38G → R: Abolishes ATPase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003881891 – 340ATPase get3Add BLAST340

Proteomic databases

PRIDEiQ4WY07

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-15800715,EBI-15800715

GO - Molecular functioni

Protein-protein interaction databases

DIPiDIP-48950N
STRINGi5085.CADAFUBP00003705

Structurei

Secondary structure

1340
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi18 – 21Combined sources4
Beta strandi28 – 32Combined sources5
Beta strandi34 – 38Combined sources5
Helixi39 – 51Combined sources13
Beta strandi57 – 61Combined sources5
Helixi67 – 72Combined sources6
Beta strandi87 – 93Combined sources7
Helixi100 – 104Combined sources5
Helixi134 – 150Combined sources17
Beta strandi153 – 158Combined sources6
Turni163 – 166Combined sources4
Helixi167 – 170Combined sources4
Helixi171 – 185Combined sources15
Helixi198 – 201Combined sources4
Helixi209 – 230Combined sources22
Turni233 – 235Combined sources3
Beta strandi236 – 242Combined sources7
Helixi246 – 261Combined sources16
Beta strandi266 – 274Combined sources9
Helixi284 – 303Combined sources20
Turni304 – 306Combined sources3
Beta strandi307 – 313Combined sources7
Helixi322 – 330Combined sources9
Turni331 – 333Combined sources3

3D structure databases

ProteinModelPortaliQ4WY07
SMRiQ4WY07
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ4WY07

Family & Domainsi

Sequence similaritiesi

Belongs to the arsA ATPase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000197637
InParanoidiQ4WY07
KOiK01551
OMAiDQIADLY
OrthoDBiEOG092C33XF

Family and domain databases

CDDicd02035 ArsA, 1 hit
HAMAPiMF_03112 Asna1_Get3, 1 hit
InterProiView protein in InterPro
IPR025723 Anion-transp_ATPase-like_dom
IPR016300 ATPase_ArsA/GET3
IPR027542 ATPase_ArsA/GET3_euk
IPR027417 P-loop_NTPase
PANTHERiPTHR10803 PTHR10803, 1 hit
PTHR10803:SF3 PTHR10803:SF3, 1 hit
PfamiView protein in Pfam
PF02374 ArsA_ATPase, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00345 GET3_arsA_TRC40, 1 hit

Sequencei

Sequence statusi: Complete.

Q4WY07-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSTAVVHGD DLMEPTLQSI LSQKTLRWIF VGGKGGVGKT TTSCSLAIQL
60 70 80 90 100
AKVRKSVLLI STDPAHNLSD AFGQKFGKEA RLVDGYSNLS AMEIDPNGSI
110 120 130 140 150
QDLLASGDSQ GDDPLAGLGM GNMMQDLAFS IPGVDEAMSF AEVLKQVKSL
160 170 180 190 200
SYEVIVFDTA PTGHTLRFLQ FPTVLEKALA KLSQLSSQFG PMLNSILGAR
210 220 230 240 250
GGLPGGQNID ELLQKMESLR ETISEVNTQF KNPDMTTFVC VCIAEFLSLY
260 270 280 290 300
ETERMIQELT SYGIDTHAIV VNQLLFPKEG SGCEQCNARR KMQKKYLEQI
310 320 330 340
EELYEDFNVV RMPLLVEEVR GKEKLEKFSE MLVHPYVPPQ
Length:340
Mass (Da):37,440
Last modified:April 17, 2007 - v2
Checksum:i9F6B277957FFE461
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAHF01000002 Genomic DNA Translation: EAL92446.2
RefSeqiXP_754484.2, XM_749391.2

Genome annotation databases

EnsemblFungiiEAL92446; EAL92446; AFUA_3G11350
GeneIDi3512551
KEGGiafm:AFUA_3G11350

Similar proteinsi

Entry informationi

Entry nameiGET3_ASPFU
AccessioniPrimary (citable) accession number: Q4WY07
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 3, 2009
Last sequence update: April 17, 2007
Last modified: June 20, 2018
This is version 85 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

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