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Protein

Thiamine thiazole synthase

Gene

thiA

Organism
Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in biosynthesis of the thiamine precursor thiazole. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylic acid (ADT), an adenylated thiazole intermediate. The reaction includes an iron-dependent sulfide transfer from a conserved cysteine residue of the protein to a thiazole intermediate. The enzyme can only undergo a single turnover, which suggests it is a suicide enzyme. May have additional roles in adaptation to various stress conditions and in DNA damage tolerance.UniRule annotation

Cofactori

Fe cationUniRule annotationNote: Binds 1 Fe cation per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei87Substrate; via amide nitrogenUniRule annotation1
Binding sitei116Substrate; via amide nitrogenUniRule annotation1
Binding sitei184Substrate; via amide nitrogen and carbonyl oxygenUniRule annotation1
Binding sitei223SubstrateUniRule annotation1
Binding sitei238SubstrateUniRule annotation1
Binding sitei290Substrate; via amide nitrogenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processThiamine biosynthesis
LigandIron, Metal-binding, NAD

Names & Taxonomyi

Protein namesi
Recommended name:
Thiamine thiazole synthaseUniRule annotation
Alternative name(s):
Thiazole biosynthetic enzymeUniRule annotation
Gene namesi
Name:thiAUniRule annotation
ORF Names:AFUA_6G08360
OrganismiNeosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
Taxonomic identifieri330879 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000002530 Componentsi: Chromosome 6, Unassembled WGS sequence

Organism-specific databases

EuPathDBiFungiDB:Afu6g08360

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004158761 – 332Thiamine thiazole synthaseAdd BLAST332

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2212,3-didehydroalanine (Cys)UniRule annotation1

Post-translational modificationi

During the catalytic reaction, a sulfide is transferred from Cys-221 to a reaction intermediate, generating a dehydroalanine residue.UniRule annotation

Proteomic databases

PRIDEiQ4WMX7

Interactioni

Subunit structurei

Homooctamer.UniRule annotation

Protein-protein interaction databases

STRINGi5085.CADAFUBP00007239

Structurei

3D structure databases

ProteinModelPortaliQ4WMX7
SMRiQ4WMX7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni108 – 109Substrate bindingUniRule annotation2
Regioni300 – 302Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the THI4 family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000106048
InParanoidiQ4WMX7
KOiK03146
OMAiMWGGGMM
OrthoDBiEOG092C3CAV

Family and domain databases

Gene3Di3.50.50.60, 1 hit
HAMAPiMF_03158 THI4, 1 hit
InterProiView protein in InterPro
IPR036188 FAD/NAD-bd_sf
IPR027495 Sti35
IPR002922 Thi4_fam
SUPFAMiSSF51905 SSF51905, 1 hit
TIGRFAMsiTIGR00292 TIGR00292, 1 hit

Sequencei

Sequence statusi: Complete.

Q4WMX7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSPPAAIFEP AVAPSPSLKA KVLVPETVPA SGTSQTHLLD HFGGKWDDFK
60 70 80 90 100
FAPIRESQVS RAMTRRYFQD LDKYAESDIV IVGAGSCGLS TAYVLAKARP
110 120 130 140 150
DLKIAIIEAS VSPGGGAWLG GQLFSAMVLR RPAEVFLNEI GVPFEEDPAN
160 170 180 190 200
PNFVVVKHAS LFTSTLMSKV LSFPNVKLFN ATAVEDLVTR PSASGDAKDT
210 220 230 240 250
QIAGVVVNWT LVTLHHDDHS CMDPNTINAP VVISTTGHDG PFGAFCAKRL
260 270 280 290 300
VSMNTVDKLG GMRGLDMNSA EDAIVKNTRE VAKGLIIGGM ELSEIDGFNR
310 320 330
MGPTFGAMVL SGVKAAEEAL KVFDQRQREC AE
Length:332
Mass (Da):35,492
Last modified:July 5, 2005 - v1
Checksum:i3F70D7BA8543D00F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAHF01000006 Genomic DNA Translation: EAL88687.1
RefSeqiXP_750725.1, XM_745632.1

Genome annotation databases

EnsemblFungiiEAL88687; EAL88687; AFUA_6G08360
GeneIDi3508853
KEGGiafm:AFUA_6G08360

Similar proteinsi

Entry informationi

Entry nameiTHI4_ASPFU
AccessioniPrimary (citable) accession number: Q4WMX7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 22, 2012
Last sequence update: July 5, 2005
Last modified: June 20, 2018
This is version 78 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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