UniProtKB - Q4WAW7 (FTMB_ASPFU)
Tryprostatin B synthase
ftmPT1
Functioni
Brevianamide F prenyltransferase; part of the gene cluster that mediates the biosynthesis of fumitremorgins, indole alkaloids that carry not only intriguing chemical structures, but also interesting biological and pharmacological activities (PubMed:16000710, PubMed:23649274).
The biosynthesis of fumitremorgin-type alkaloids begins by condensation of the two amino acids L-tryptophan and L-proline to brevianamide F, catalyzed by the non-ribosomal peptide synthetase ftmA (PubMed:16755625).
Brevianamide F is then prenylated by the prenyltransferase ftmPT1/ftmB in the presence of dimethylallyl diphosphate, resulting in the formation of tryprostatin B (PubMed:16000710, PubMed:21105662, PubMed:23090579).
The three cytochrome P450 monooxygenases, ftmP450-1/ftmC, ftmP450-2/ftmE and ftmP450-3/FtmG, are responsible for the conversion of tryprostatin B to 6-hydroxytryprostatin B, tryprostatin A to fumitremorgin C and fumitremorgin C to 12,13-dihydroxyfumitremorgin C, respectively (PubMed:19226505).
The putative methyltransferase ftmMT/ftmD is expected for the conversion of 6-hydroxytryprostatin B to tryprostatin A (Probable). FtmPT2/FtmH catalyzes the prenylation of 12,13-dihydroxyfumitre-morgin C in the presence of dimethylallyl diphosphate, resulting in the formation of fumitremorgin B (PubMed:18683158).
Fumitremorgin B is further converted to verruculogen by ftmOx1/ftmF via the insertion of an endoperoxide bond between the two prenyl moieties (PubMed:19763315).
In some fungal species, verruculogen is further converted to fumitremorgin A, but the enzymes involved in this step have not been identified yet (Probable).
Curated8 PublicationsCaution
Catalytic activityi
- EC:2.5.1.1061 Publication
Kineticsi
- KM=55 µM for brevianamide F1 Publication
- KM=74 µM for dimethylallyl diphosphate1 Publication
: Mycotoxin biosynthesis Pathwayi
This protein is involved in Mycotoxin biosynthesis.2 PublicationsView all proteins of this organism that are known to be involved in Mycotoxin biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 94 | Brevianamide F1 Publication | 1 | |
Binding sitei | 102 | Brevianamide F1 Publication | 1 | |
Binding sitei | 113 | Dimethylallyl diphosphate1 Publication | 1 | |
Sitei | 115 | Required for regioselectivity1 Publication | 1 | |
Binding sitei | 201 | Dimethylallyl diphosphate1 Publication | 1 | |
Binding sitei | 203 | Dimethylallyl diphosphate1 Publication | 1 | |
Binding sitei | 205 | Brevianamide F1 Publication | 1 | |
Binding sitei | 294 | Dimethylallyl diphosphate1 Publication | 1 | |
Binding sitei | 296 | Dimethylallyl diphosphate1 Publication | 1 | |
Binding sitei | 380 | Dimethylallyl diphosphate1 Publication | 1 | |
Binding sitei | 382 | Dimethylallyl diphosphate1 Publication | 1 | |
Binding sitei | 446 | Dimethylallyl diphosphate1 Publication | 1 | |
Binding sitei | 450 | Dimethylallyl diphosphate1 Publication | 1 |
GO - Molecular functioni
- prenyltransferase activity Source: UniProtKB-KW
GO - Biological processi
- verruculogen biosynthetic process Source: GO_Central
Keywordsi
Molecular function | Prenyltransferase, Transferase |
Biological process | Virulence |
Enzyme and pathway databases
BRENDAi | 2.5.1.106, 508 |
Protein family/group databases
MEROPSi | M77.004 |
Names & Taxonomyi
Protein namesi | Recommended name: Tryprostatin B synthase1 Publication (EC:2.5.1.1061 Publication)Alternative name(s): Brevianamide F prenyltransferase1 Publication Fumitremorgin biosynthesis protein B1 Publication |
Gene namesi | Name:ftmPT11 Publication Synonyms:ftmB1 Publication ORF Names:AFUA_8G00210 |
Organismi | Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus) |
Taxonomic identifieri | 330879 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Aspergillaceae › Aspergillus › Aspergillus subgen. Fumigati › |
Proteomesi |
|
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 102 | E → Q: Abolishes tryprostatin B synthase activity. 1 Publication | 1 | |
Mutagenesisi | 115 | G → T: Retains ability to consume brevianamide F but mediates formation of a different product that carries a reverse prenyl moiety at the C-3 of the indole nucleus. 1 Publication | 1 | |
Mutagenesisi | 279 | H → F: Slightly affects tryprostatin B synthase activity. 1 Publication | 1 |
Chemistry databases
ChEMBLi | CHEMBL5635 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000424111 | 1 – 464 | Tryprostatin B synthaseAdd BLAST | 464 |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | Q4WAW7 |
SMRi | Q4WAW7 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | ENOG502S2XP, Eukaryota |
HOGENOMi | CLU_037431_0_0_1 |
InParanoidi | Q4WAW7 |
OrthoDBi | 1531660at2759 |
Family and domain databases
CDDi | cd13929, PT-DMATS_CymD, 1 hit |
InterProi | View protein in InterPro IPR033964, Aro_prenylTrfase IPR017795, Aro_prenylTrfase_DMATS IPR012148, DMATS-type_fun |
PANTHERi | PTHR40627, PTHR40627, 1 hit |
Pfami | View protein in Pfam PF11991, Trp_DMAT, 1 hit |
PIRSFi | PIRSF000509, Trp_DMAT, 1 hit |
SFLDi | SFLDS00036, Aromatic_Prenyltransferase, 1 hit |
TIGRFAMsi | TIGR03429, arom_pren_DMATS, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MPPAPPDQKP CHQLQPAPYR ALSESILFGS VDEERWWHST APILSRLLIS
60 70 80 90 100
SNYDVDVQYK YLSLYRHLVL PALGPYPQRD PETGIIATQW RSGMVLTGLP
110 120 130 140 150
IEFSNNVARA LIRIGVDPVT ADSGTAQDPF NTTRPKVYLE TAARLLPGVD
160 170 180 190 200
LTRFYEFETE LVITKAEEAV LQANPDLFRS PWKSQILTAM DLQKSGTVLV
210 220 230 240 250
KAYFYPQPKS AVTGRSTEDL LVNAIRKVDR EGRFETQLAN LQRYIERRRR
260 270 280 290 300
GLHVPGVTAD KPPATAADKA FDACSFFPHF LSTDLVEPGK SRVKFYASER
310 320 330 340 350
HVNLQMVEDI WTFGGLRRDP DALRGLELLR HFWADIQMRE GYYTMPRGFC
360 370 380 390 400
ELGKSSAGFE APMMFHFHLD GSQSPFPDPQ MYVCVFGMNS RKLVEGLTTF
410 420 430 440 450
YRRVGWEEMA SHYQGNFLAN YPDEDFEKAA HLCAYVSFAY KNGGAYVTLY
460
NHSFNPVGDV SFPN
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 415 | G → A in AAX56314 (PubMed:16000710).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY861687 mRNA Translation: AAX56314.1 AAHF01000014 Genomic DNA Translation: EAL85145.1 Different initiation. |
RefSeqi | XP_747183.1, XM_742090.1 |
Genome annotation databases
GeneIDi | 3504530 |
KEGGi | afm:AFUA_8G00210 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY861687 mRNA Translation: AAX56314.1 AAHF01000014 Genomic DNA Translation: EAL85145.1 Different initiation. |
RefSeqi | XP_747183.1, XM_742090.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3O24 | X-ray | 2.50 | A | 1-464 | [»] | |
3O2K | X-ray | 2.40 | A | 1-464 | [»] | |
AlphaFoldDBi | Q4WAW7 | |||||
SMRi | Q4WAW7 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 746128.CADAFUBP00008411 |
Chemistry databases
ChEMBLi | CHEMBL5635 |
Protein family/group databases
MEROPSi | M77.004 |
Genome annotation databases
GeneIDi | 3504530 |
KEGGi | afm:AFUA_8G00210 |
Phylogenomic databases
eggNOGi | ENOG502S2XP, Eukaryota |
HOGENOMi | CLU_037431_0_0_1 |
InParanoidi | Q4WAW7 |
OrthoDBi | 1531660at2759 |
Enzyme and pathway databases
BRENDAi | 2.5.1.106, 508 |
Miscellaneous databases
PROi | PR:Q4WAW7 |
Family and domain databases
CDDi | cd13929, PT-DMATS_CymD, 1 hit |
InterProi | View protein in InterPro IPR033964, Aro_prenylTrfase IPR017795, Aro_prenylTrfase_DMATS IPR012148, DMATS-type_fun |
PANTHERi | PTHR40627, PTHR40627, 1 hit |
Pfami | View protein in Pfam PF11991, Trp_DMAT, 1 hit |
PIRSFi | PIRSF000509, Trp_DMAT, 1 hit |
SFLDi | SFLDS00036, Aromatic_Prenyltransferase, 1 hit |
TIGRFAMsi | TIGR03429, arom_pren_DMATS, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | FTMB_ASPFU | |
Accessioni | Q4WAW7Primary (citable) accession number: Q4WAW7 Secondary accession number(s): Q4G2I1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 16, 2013 |
Last sequence update: | October 16, 2013 | |
Last modified: | May 25, 2022 | |
This is version 89 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families