UniProtKB - Q4VCS5 (AMOT_HUMAN)
Protein
Angiomotin
Gene
AMOT
Organism
Homo sapiens (Human)
Status
Functioni
Plays a central role in tight junction maintenance via the complex formed with ARHGAP17, which acts by regulating the uptake of polarity proteins at tight junctions. Appears to regulate endothelial cell migration and tube formation. May also play a role in the assembly of endothelial cell-cell junctions.2 Publications
Miscellaneous
'Motus' means 'motility' in Latin.
GO - Molecular functioni
- angiostatin binding Source: UniProtKB
- signaling receptor activity Source: MGI
GO - Biological processi
- actin cytoskeleton organization Source: GO_Central
- angiogenesis Source: GO_Central
- blood vessel endothelial cell migration Source: Ensembl
- cell-cell junction assembly Source: UniProtKB
- cell migration involved in gastrulation Source: Ensembl
- cellular protein localization Source: MGI
- chemotaxis Source: Ensembl
- establishment of cell polarity involved in ameboidal cell migration Source: GO_Central
- gastrulation with mouth forming second Source: Ensembl
- hippo signaling Source: MGI
- in utero embryonic development Source: Ensembl
- negative regulation of angiogenesis Source: MGI
- negative regulation of GTPase activity Source: Ensembl
- negative regulation of vascular permeability Source: UniProtKB
- positive regulation of blood vessel endothelial cell migration Source: UniProtKB
- positive regulation of cell size Source: UniProtKB
- positive regulation of embryonic development Source: Ensembl
- positive regulation of stress fiber assembly Source: UniProtKB
- regulation of cell migration Source: MGI
- regulation of small GTPase mediated signal transduction Source: Ensembl
- vasculogenesis Source: Ensembl
Enzyme and pathway databases
PathwayCommonsi | Q4VCS5 |
Reactomei | R-HSA-2028269, Signaling by Hippo [Q4VCS5-1] |
SIGNORi | Q4VCS5 |
Names & Taxonomyi
Protein namesi | Recommended name: Angiomotin |
Gene namesi | Name:AMOT Synonyms:KIAA1071 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:17810, AMOT |
MIMi | 300410, gene |
neXtProti | NX_Q4VCS5 |
VEuPathDBi | HostDB:ENSG00000126016.13 |
Subcellular locationi
Other locations
- tight junction 1 Publication
Note: Localized on the cell surface. May act as a transmembrane protein.
Cytoskeleton
- actin filament Source: UniProtKB
- stress fiber Source: UniProtKB
Cytosol
- cytosol Source: Reactome
Nucleus
- nucleoplasm Source: HPA
Plasma Membrane
- external side of plasma membrane Source: MGI
- plasma membrane Source: GO_Central
Other locations
- bicellular tight junction Source: UniProtKB
- cell junction Source: HPA
- cell surface Source: UniProtKB
- cytoplasm Source: UniProtKB
- cytoplasmic vesicle Source: GO_Central
- endocytic vesicle Source: MGI
- integral component of membrane Source: UniProtKB
- lamellipodium Source: UniProtKB
- ruffle Source: MGI
Keywords - Cellular componenti
Cell junction, Tight junctionPathology & Biotechi
Organism-specific databases
DisGeNETi | 154796 |
OpenTargetsi | ENSG00000126016 |
PharmGKBi | PA24773 |
Miscellaneous databases
Pharosi | Q4VCS5, Tbio |
Chemistry databases
ChEMBLi | CHEMBL3392949 |
Genetic variation databases
BioMutai | AMOT |
DMDMi | 74753814 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000190668 | 1 – 1084 | AngiomotinAdd BLAST | 1084 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 594 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Cross-linki | 595 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Modified residuei | 714 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Polyubiquitinated by NEDD4, NEDD4L and ITCH, leading to proteasomal degradation.1 Publication
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q4VCS5 |
jPOSTi | Q4VCS5 |
MassIVEi | Q4VCS5 |
MaxQBi | Q4VCS5 |
PaxDbi | Q4VCS5 |
PeptideAtlasi | Q4VCS5 |
PRIDEi | Q4VCS5 |
ProteomicsDBi | 62307 [Q4VCS5-1] 62308 [Q4VCS5-2] |
PTM databases
iPTMneti | Q4VCS5 |
PhosphoSitePlusi | Q4VCS5 |
Expressioni
Tissue specificityi
Expressed in placenta and skeletal muscle. Found in the endothelial cells of capillaries as well as larger vessels of the placenta.1 Publication
Gene expression databases
Bgeei | ENSG00000126016, Expressed in quadriceps femoris and 141 other tissues |
ExpressionAtlasi | Q4VCS5, baseline and differential |
Genevisiblei | Q4VCS5, HS |
Organism-specific databases
HPAi | ENSG00000126016, Tissue enhanced (ductus deferens, epididymis) |
Interactioni
Subunit structurei
Component of a complex whose core is composed of ARHGAP17, AMOT, MPP5/PALS1, PATJ and PARD3/PAR3.
Interacts with MAGI1.
Isoform 1 interacts with angiostatin.
2 PublicationsBinary interactionsi
Hide detailsQ4VCS5
With | #Exp. | IntAct |
---|---|---|
ARHGAP17 [Q68EM7] | 2 | EBI-2511319,EBI-1642807 |
IKBKG [Q9Y6K9] | 3 | EBI-2511319,EBI-81279 |
NF2 [P35240] | 9 | EBI-2511319,EBI-1014472 |
Nf2 [P46662] from Mus musculus. | 2 | EBI-2511319,EBI-644586 |
Isoform 1 [Q4VCS5-1]
With | #Exp. | IntAct |
---|---|---|
NF2 [P35240] | 2 | EBI-3903812,EBI-1014472 |
Isoform 2 [Q4VCS5-2]
GO - Molecular functioni
- angiostatin binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 127557, 161 interactors |
CORUMi | Q4VCS5 |
DIPi | DIP-53711N |
ELMi | Q4VCS5 |
IntActi | Q4VCS5, 104 interactors |
MINTi | Q4VCS5 |
STRINGi | 9606.ENSP00000361027 |
Miscellaneous databases
RNActi | Q4VCS5, protein |
Family & Domainsi
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 429 – 689 | Sequence analysisAdd BLAST | 261 | |
Coiled coili | 721 – 751 | Sequence analysisAdd BLAST | 31 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 1081 – 1084 | PDZ-binding | 4 |
Domaini
The coiled coil domain interacts directly with the BAR domain of ARHGAP17.1 Publication
The angiostatin binding domain (871-1005) allows the binding to angiostatin.1 Publication
Sequence similaritiesi
Belongs to the angiomotin family.Curated
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | ENOG502QVI5, Eukaryota |
GeneTreei | ENSGT00940000159055 |
InParanoidi | Q4VCS5 |
OMAi | GDYRAEY |
OrthoDBi | 369983at2759 |
PhylomeDBi | Q4VCS5 |
TreeFami | TF333368 |
Family and domain databases
InterProi | View protein in InterPro IPR009114, Angiomotin IPR024646, Angiomotin_C |
Pfami | View protein in Pfam PF12240, Angiomotin_C, 1 hit |
PRINTSi | PR01807, ANGIOMOTIN |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q4VCS5-1) [UniParc]FASTAAdd to basket
Also known as: p130
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MRNSEEQPSG GTTVLQRLLQ EQLRYGNPSE NRSLLAIHQQ ATGNGPPFPS
60 70 80 90 100
GSGNPGPQSD VLSPQDHHQQ LVAHAARQEP QGQEIQSENL IMEKQLSPRM
110 120 130 140 150
QNNEELPTYE EAKVQSQYFR GQQHASVGAA FYVTGVTNQK MRTEGRPSVQ
160 170 180 190 200
RLNPGKMHQD EGLRDLKQGH VRSLSERLMQ MSLATSGVKA HPPVTSAPLS
210 220 230 240 250
PPQPNDLYKN PTSSSEFYKA QGPLPNQHSL KGMEHRGPPP EYPFKGMPPQ
260 270 280 290 300
SVVCKPQEPG HFYSEHRLNQ PGRTEGQLMR YQHPPEYGAA RPAQDISLPL
310 320 330 340 350
SARNSQPHSP TSSLTSGGSL PLLQSPPSTR LSPARHPLVP NQGDHSAHLP
360 370 380 390 400
RPQQHFLPNQ AHQGDHYRLS QPGLSQQQQQ QQQQHHHHHH HQQQQQQQPQ
410 420 430 440 450
QQPGEAYSAM PRAQPSSASY QPVPADPFAI VSRAQQMVEI LSDENRNLRQ
460 470 480 490 500
ELEGCYEKVA RLQKVETEIQ RVSEAYENLV KSSSKREALE KAMRNKLEGE
510 520 530 540 550
IRRMHDFNRD LRERLETANK QLAEKEYEGS EDTRKTISQL FAKNKESQRE
560 570 580 590 600
KEKLEAELAT ARSTNEDQRR HIEIRDQALS NAQAKVVKLE EELKKKQVYV
610 620 630 640 650
DKVEKMQQAL VQLQAACEKR EQLEHRLRTR LERELESLRI QQRQGNCQPT
660 670 680 690 700
NVSEYNAAAL MELLREKEER ILALEADMTK WEQKYLEENV MRHFALDAAA
710 720 730 740 750
TVAAQRDTTV ISHSPNTSYD TALEARIQKE EEEILMANKR CLDMEGRIKT
760 770 780 790 800
LHAQIIEKDA MIKVLQQRSR KEPSKTEQLS CMRPAKSLMS ISNAGSGLLS
810 820 830 840 850
HSSTLTGSPI MEEKRDDKSW KGSLGILLGG DYRAEYVPST PSPVPPSTPL
860 870 880 890 900
LSAHSKTGSR DCSTQTERGT ESNKTAAVAP ISVPAPVAAA ATAAAITATA
910 920 930 940 950
ATITTTMVAA APVAVAAAAA PAAAAAPSPA TAAATAAAVS PAAAGQIPAA
960 970 980 990 1000
ASVASAAAVA PSAAAAAAVQ VAPAAPAPVP APALVPVPAP AAAQASAPAQ
1010 1020 1030 1040 1050
TQAPTSAPAV APTPAPTPTP AVAQAEVPAS PATGPGPHRL SIPSLTCNPD
1060 1070 1080
KTDGPVFHSN TLERKTPIQI LGQEPDAEMV EYLI
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA6NP16 | A6NP16_HUMAN | Angiomotin | AMOT | 597 | Annotation score: | ||
E7ERM3 | E7ERM3_HUMAN | Angiomotin | AMOT | 852 | Annotation score: |
Sequence cautioni
The sequence AAH94712 differs from that shown. Contaminating sequence. Potential poly-A sequence.Curated
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_015709 | 1 – 409 | Missing in isoform 2. 2 PublicationsAdd BLAST | 409 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF286598 mRNA Translation: AAG01851.1 AY987378 mRNA Translation: AAY24451.1 AB028994 mRNA Translation: BAA83023.3 BC094712 mRNA Translation: AAH94712.1 Sequence problems. |
CCDSi | CCDS14563.1 [Q4VCS5-2] CCDS48154.1 [Q4VCS5-1] |
RefSeqi | NP_001106962.1, NM_001113490.1 [Q4VCS5-1] NP_573572.1, NM_133265.2 [Q4VCS5-2] XP_005262144.1, XM_005262087.1 [Q4VCS5-1] XP_005262147.1, XM_005262090.1 [Q4VCS5-2] XP_011529177.1, XM_011530875.2 [Q4VCS5-1] XP_016884778.1, XM_017029289.1 [Q4VCS5-2] |
Genome annotation databases
Ensembli | ENST00000304758; ENSP00000305557; ENSG00000126016 [Q4VCS5-2] ENST00000371959; ENSP00000361027; ENSG00000126016 [Q4VCS5-1] ENST00000524145; ENSP00000429013; ENSG00000126016 [Q4VCS5-1] |
GeneIDi | 154796 |
KEGGi | hsa:154796 |
UCSCi | uc004epr.4, human [Q4VCS5-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Web resourcesi
Atlas of Genetics and Cytogenetics in Oncology and Haematology |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF286598 mRNA Translation: AAG01851.1 AY987378 mRNA Translation: AAY24451.1 AB028994 mRNA Translation: BAA83023.3 BC094712 mRNA Translation: AAH94712.1 Sequence problems. |
CCDSi | CCDS14563.1 [Q4VCS5-2] CCDS48154.1 [Q4VCS5-1] |
RefSeqi | NP_001106962.1, NM_001113490.1 [Q4VCS5-1] NP_573572.1, NM_133265.2 [Q4VCS5-2] XP_005262144.1, XM_005262087.1 [Q4VCS5-1] XP_005262147.1, XM_005262090.1 [Q4VCS5-2] XP_011529177.1, XM_011530875.2 [Q4VCS5-1] XP_016884778.1, XM_017029289.1 [Q4VCS5-2] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6JJX | X-ray | 2.00 | C/D | 272-293 | [»] | |
SMRi | Q4VCS5 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 127557, 161 interactors |
CORUMi | Q4VCS5 |
DIPi | DIP-53711N |
ELMi | Q4VCS5 |
IntActi | Q4VCS5, 104 interactors |
MINTi | Q4VCS5 |
STRINGi | 9606.ENSP00000361027 |
Chemistry databases
ChEMBLi | CHEMBL3392949 |
PTM databases
iPTMneti | Q4VCS5 |
PhosphoSitePlusi | Q4VCS5 |
Genetic variation databases
BioMutai | AMOT |
DMDMi | 74753814 |
Proteomic databases
EPDi | Q4VCS5 |
jPOSTi | Q4VCS5 |
MassIVEi | Q4VCS5 |
MaxQBi | Q4VCS5 |
PaxDbi | Q4VCS5 |
PeptideAtlasi | Q4VCS5 |
PRIDEi | Q4VCS5 |
ProteomicsDBi | 62307 [Q4VCS5-1] 62308 [Q4VCS5-2] |
Protocols and materials databases
Antibodypediai | 29579, 416 antibodies |
DNASUi | 154796 |
Genome annotation databases
Ensembli | ENST00000304758; ENSP00000305557; ENSG00000126016 [Q4VCS5-2] ENST00000371959; ENSP00000361027; ENSG00000126016 [Q4VCS5-1] ENST00000524145; ENSP00000429013; ENSG00000126016 [Q4VCS5-1] |
GeneIDi | 154796 |
KEGGi | hsa:154796 |
UCSCi | uc004epr.4, human [Q4VCS5-1] |
Organism-specific databases
CTDi | 154796 |
DisGeNETi | 154796 |
GeneCardsi | AMOT |
HGNCi | HGNC:17810, AMOT |
HPAi | ENSG00000126016, Tissue enhanced (ductus deferens, epididymis) |
MIMi | 300410, gene |
neXtProti | NX_Q4VCS5 |
OpenTargetsi | ENSG00000126016 |
PharmGKBi | PA24773 |
VEuPathDBi | HostDB:ENSG00000126016.13 |
HUGEi | Search... |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502QVI5, Eukaryota |
GeneTreei | ENSGT00940000159055 |
InParanoidi | Q4VCS5 |
OMAi | GDYRAEY |
OrthoDBi | 369983at2759 |
PhylomeDBi | Q4VCS5 |
TreeFami | TF333368 |
Enzyme and pathway databases
PathwayCommonsi | Q4VCS5 |
Reactomei | R-HSA-2028269, Signaling by Hippo [Q4VCS5-1] |
SIGNORi | Q4VCS5 |
Miscellaneous databases
BioGRID-ORCSi | 154796, 3 hits in 502 CRISPR screens |
ChiTaRSi | AMOT, human |
GeneWikii | AMOT |
GenomeRNAii | 154796 |
Pharosi | Q4VCS5, Tbio |
PROi | PR:Q4VCS5 |
RNActi | Q4VCS5, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000126016, Expressed in quadriceps femoris and 141 other tissues |
ExpressionAtlasi | Q4VCS5, baseline and differential |
Genevisiblei | Q4VCS5, HS |
Family and domain databases
InterProi | View protein in InterPro IPR009114, Angiomotin IPR024646, Angiomotin_C |
Pfami | View protein in Pfam PF12240, Angiomotin_C, 1 hit |
PRINTSi | PR01807, ANGIOMOTIN |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | AMOT_HUMAN | |
Accessioni | Q4VCS5Primary (citable) accession number: Q4VCS5 Secondary accession number(s): Q504X5, Q9HD27, Q9UPT1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 27, 2005 |
Last sequence update: | July 5, 2005 | |
Last modified: | February 10, 2021 | |
This is version 150 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome X
Human chromosome X: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families