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Protein

Pikachurin

Gene

Egflam

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in both the retinal photoreceptor ribbon synapse formation and physiological functions of visual perception. Necessary for proper bipolar dendritic tip apposition to the photoreceptor ribbon synapse. Promotes matrix assembly and cell adhesiveness.2 Publications

GO - Molecular functioni

  • calcium ion binding Source: InterPro
  • glycosaminoglycan binding Source: MGI

GO - Biological processi

Names & Taxonomyi

Protein namesi
Recommended name:
Pikachurin
Alternative name(s):
EGF-like, fibronectin type-III and laminin G-like domain-containing protein
Nectican
Gene namesi
Name:Egflam
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:2146149 Egflam

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Extracellular matrix, Secreted, Synapse

Pathology & Biotechi

Disruption phenotypei

No morphological abnormalities. Knockout mice are viable and fertile. However, the terminal of bipolar cells do not appose to the synapse terminals in the rod photoreceptor ribbon synapses. The signal transmission from the rod photoreceptor to the rod bipolar cells is less sensitive and is delayed compared to the wild type mouse. The signal transmission from cone photoreceptors to the cone bipolar cells is also impaired. These mice show reduced visual function.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
ChainiPRO_000030680425 – 1017PikachurinAdd BLAST993

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi47N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi347 ↔ 358By similarity
Disulfide bondi352 ↔ 369By similarity
Disulfide bondi371 ↔ 380By similarity
Disulfide bondi534 ↔ 564By similarity
Disulfide bondi569 ↔ 580By similarity
Disulfide bondi574 ↔ 590By similarity
Disulfide bondi592 ↔ 601By similarity
Disulfide bondi788 ↔ 799By similarity
Disulfide bondi793 ↔ 808By similarity
Disulfide bondi810 ↔ 819By similarity
Disulfide bondi987 ↔ 1014By similarity

Post-translational modificationi

O-glycosylated; contains chondroitin sulfate and heparan sulfate.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ4VBE4
PRIDEiQ4VBE4

PTM databases

PhosphoSitePlusiQ4VBE4

Expressioni

Tissue specificityi

Expressed in the outer plexiform layer (first synaptic region) but not in the inner plexiform layer (second synaptic region) of the retina (at protein level). Strongly expressed in the photoreceptor layer of the retina. Moderately expressed in pineal gland and brain. Weakly expressed in lung and ovary.1 Publication

Developmental stagei

Expressed in the apical side of the neuroblastic layer (NBL) of the retina at E14.5 and E17.5. At E16.5, present in rib cartilage and hair follicle (at protein level).2 Publications

Gene expression databases

BgeeiENSMUSG00000042961 Expressed in 165 organ(s), highest expression level in retina
ExpressionAtlasiQ4VBE4 baseline and differential
GenevisibleiQ4VBE4 MM

Interactioni

Subunit structurei

Interacts with DAG1 alpha-dystroglycan.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Dag1Q621652EBI-2025048,EBI-2025154

Protein-protein interaction databases

IntActiQ4VBE4, 4 interactors
STRINGi10090.ENSMUSP00000055599

Structurei

3D structure databases

ProteinModelPortaliQ4VBE4
SMRiQ4VBE4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini37 – 136Fibronectin type-III 1PROSITE-ProRule annotationAdd BLAST100
Domaini144 – 239Fibronectin type-III 2PROSITE-ProRule annotationAdd BLAST96
Domaini343 – 381EGF-like 1PROSITE-ProRule annotationAdd BLAST39
Domaini386 – 564Laminin G-like 1PROSITE-ProRule annotationAdd BLAST179
Domaini565 – 602EGF-like 2PROSITE-ProRule annotationAdd BLAST38
Domaini609 – 788Laminin G-like 2PROSITE-ProRule annotationAdd BLAST180
Domaini784 – 820EGF-like 3PROSITE-ProRule annotationAdd BLAST37
Domaini835 – 1014Laminin G-like 3PROSITE-ProRule annotationAdd BLAST180

Keywords - Domaini

EGF-like domain, Repeat, Signal

Phylogenomic databases

eggNOGiKOG0613 Eukaryota
KOG3509 Eukaryota
ENOG410XRPX LUCA
GeneTreeiENSGT00930000150942
HOGENOMiHOG000112344
HOVERGENiHBG107839
InParanoidiQ4VBE4
OMAiLGHWHEL
OrthoDBiEOG091G048M
PhylomeDBiQ4VBE4
TreeFamiTF326548

Family and domain databases

CDDicd00063 FN3, 2 hits
Gene3Di2.60.40.10, 2 hits
InterProiView protein in InterPro
IPR013320 ConA-like_dom_sf
IPR001881 EGF-like_Ca-bd_dom
IPR013032 EGF-like_CS
IPR000742 EGF-like_dom
IPR003961 FN3_dom
IPR036116 FN3_sf
IPR013783 Ig-like_fold
IPR001791 Laminin_G
PfamiView protein in Pfam
PF00008 EGF, 2 hits
PF00041 fn3, 2 hits
PF00054 Laminin_G_1, 1 hit
PF02210 Laminin_G_2, 2 hits
SMARTiView protein in SMART
SM00181 EGF, 3 hits
SM00179 EGF_CA, 2 hits
SM00060 FN3, 2 hits
SM00282 LamG, 3 hits
SUPFAMiSSF49265 SSF49265, 1 hit
SSF49899 SSF49899, 3 hits
PROSITEiView protein in PROSITE
PS00022 EGF_1, 3 hits
PS01186 EGF_2, 2 hits
PS50026 EGF_3, 3 hits
PS50853 FN3, 2 hits
PS50025 LAM_G_DOMAIN, 3 hits

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: Q4VBE4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MDLISTFSLH FLLLACSLPP GAVSLRTALR KSGKVGPPLD IKLGALNCTA
60 70 80 90 100
FSIQWKTPKR SGSSIIGYTV FYSEVGSDKS LRERSHNVPV GQDTLITEEV
110 120 130 140 150
IGDLKPGTEY QVSVAAYSQT GKGRLSFPRH VTTLSQDSCL PPAAPQQPHV
160 170 180 190 200
LVVSDSEVAL SWRPGENEGS APIQSYSVEF IRPDFDKSWT IIQERLQMDS
210 220 230 240 250
MVIKGLDPDT NYQFAVKAMN AHGFSPRSWP SNTVRTLGPG EAGSGHYGPG
260 270 280 290 300
YITNPGVSED DDGSEDELDL DVSFEEVKPL PATKVGNKKF SVESKKTSVS
310 320 330 340 350
NSVMGSRLAQ PTSASLHETT VAIPPTPAQR KGKNSVAMMS RLFDMSCDET
360 370 380 390 400
LCSADSFCVN DYAWGGSRCH CNLGKGGEAC SEDIFIQYPQ FFGHSYVTFE
410 420 430 440 450
PLKNSYQAFQ VTLEFRAEAE DGLLLYCGES EHGRGDFMSL ALIRRSLHFR
460 470 480 490 500
FNCGTGIAII ISETKIKLGA WHTVTLYRDG LNGMLQLNNG TPVTGQSQGQ
510 520 530 540 550
YSKITFRTPL YLGGAPSAYW LVRATGTNRG FQGCVQSLSV NGKKIDMRPW
560 570 580 590 600
PLGKALNGAD VGECSSGICD EASCIHGGTC AAIKADSYIC LCPLGFRGRH
610 620 630 640 650
CEDAFALTIP QFRESLRSYA ATPWPLEPQH YLSFTEFEIT FRPDSGDGVL
660 670 680 690 700
LYSYDTGSKD FLSINMAAGH VEFRFDCGSG TGVLRSEAPL TLGQWHDLRV
710 720 730 740 750
SRTAKNGILQ VDKQKVVEGM AEGGFTQIKC NTDIFIGGVP NYDDVKKNSG
760 770 780 790 800
ILHPFSGSIQ KIILNDRTIH VKHDFTSGVN VENAAHPCVG APCAHGGSCR
810 820 830 840 850
PRKEGYECDC PLGFEGLNCQ KECGNHCLNT IIEAIEIPQF IGRSYLTYDN
860 870 880 890 900
PNILKRVSGS RSNAFMRFKT TAKDGLLLWR GDSPMRPNSD FISLGLRDGA
910 920 930 940 950
LIFSYNLGSG VASIMVNGSF SDGRWHRVKA VRDGQSGKIT VDDYGARTGK
960 970 980 990 1000
SPGLMRQLNI NGALYVGGMK EIALHTNRQY LRGLVGCISH FTLSTDYHIS
1010
LVEDAVDGKN INTCGAK
Length:1,017
Mass (Da):110,735
Last modified:July 5, 2005 - v1
Checksum:iF8A21D89B251D455
GO
Isoform 2 (identifier: Q4VBE4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     822-830: ECGNHCLNT → A

Show »
Length:1,009
Mass (Da):109,834
Checksum:i80DD954F255C102B
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E0CXM4E0CXM4_MOUSE
Pikachurin
Egflam
37Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti45A → T in AAI50711 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_028482822 – 830ECGNHCLNT → A in isoform 2. 2 Publications9

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ223720 mRNA Translation: ABB48431.1
AK031136 mRNA Translation: BAC27271.1
AK033332 mRNA Translation: BAC28235.1
AK037223 mRNA Translation: BAC29762.1
AK041546 mRNA Translation: BAC30982.1
AC105969 Genomic DNA No translation available.
AC158747 Genomic DNA No translation available.
BC051455 mRNA Translation: AAH51455.1
BC095994 mRNA Translation: AAH95994.1
BC150710 mRNA Translation: AAI50711.1
CCDSiCCDS27370.1 [Q4VBE4-2]
CCDS79359.1 [Q4VBE4-1]
RefSeqiNP_001276425.1, NM_001289496.1 [Q4VBE4-1]
NP_848863.1, NM_178748.6 [Q4VBE4-2]
XP_017172117.1, XM_017316628.1 [Q4VBE4-2]
UniGeneiMm.203208

Genome annotation databases

EnsembliENSMUST00000058593; ENSMUSP00000055599; ENSMUSG00000042961 [Q4VBE4-2]
ENSMUST00000096494; ENSMUSP00000094238; ENSMUSG00000042961 [Q4VBE4-1]
GeneIDi268780
KEGGimmu:268780
UCSCiuc007vea.2 mouse [Q4VBE4-2]
uc007veb.2 mouse [Q4VBE4-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ223720 mRNA Translation: ABB48431.1
AK031136 mRNA Translation: BAC27271.1
AK033332 mRNA Translation: BAC28235.1
AK037223 mRNA Translation: BAC29762.1
AK041546 mRNA Translation: BAC30982.1
AC105969 Genomic DNA No translation available.
AC158747 Genomic DNA No translation available.
BC051455 mRNA Translation: AAH51455.1
BC095994 mRNA Translation: AAH95994.1
BC150710 mRNA Translation: AAI50711.1
CCDSiCCDS27370.1 [Q4VBE4-2]
CCDS79359.1 [Q4VBE4-1]
RefSeqiNP_001276425.1, NM_001289496.1 [Q4VBE4-1]
NP_848863.1, NM_178748.6 [Q4VBE4-2]
XP_017172117.1, XM_017316628.1 [Q4VBE4-2]
UniGeneiMm.203208

3D structure databases

ProteinModelPortaliQ4VBE4
SMRiQ4VBE4
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ4VBE4, 4 interactors
STRINGi10090.ENSMUSP00000055599

PTM databases

PhosphoSitePlusiQ4VBE4

Proteomic databases

PaxDbiQ4VBE4
PRIDEiQ4VBE4

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000058593; ENSMUSP00000055599; ENSMUSG00000042961 [Q4VBE4-2]
ENSMUST00000096494; ENSMUSP00000094238; ENSMUSG00000042961 [Q4VBE4-1]
GeneIDi268780
KEGGimmu:268780
UCSCiuc007vea.2 mouse [Q4VBE4-2]
uc007veb.2 mouse [Q4VBE4-1]

Organism-specific databases

CTDi133584
MGIiMGI:2146149 Egflam

Phylogenomic databases

eggNOGiKOG0613 Eukaryota
KOG3509 Eukaryota
ENOG410XRPX LUCA
GeneTreeiENSGT00930000150942
HOGENOMiHOG000112344
HOVERGENiHBG107839
InParanoidiQ4VBE4
OMAiLGHWHEL
OrthoDBiEOG091G048M
PhylomeDBiQ4VBE4
TreeFamiTF326548

Miscellaneous databases

PROiPR:Q4VBE4
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000042961 Expressed in 165 organ(s), highest expression level in retina
ExpressionAtlasiQ4VBE4 baseline and differential
GenevisibleiQ4VBE4 MM

Family and domain databases

CDDicd00063 FN3, 2 hits
Gene3Di2.60.40.10, 2 hits
InterProiView protein in InterPro
IPR013320 ConA-like_dom_sf
IPR001881 EGF-like_Ca-bd_dom
IPR013032 EGF-like_CS
IPR000742 EGF-like_dom
IPR003961 FN3_dom
IPR036116 FN3_sf
IPR013783 Ig-like_fold
IPR001791 Laminin_G
PfamiView protein in Pfam
PF00008 EGF, 2 hits
PF00041 fn3, 2 hits
PF00054 Laminin_G_1, 1 hit
PF02210 Laminin_G_2, 2 hits
SMARTiView protein in SMART
SM00181 EGF, 3 hits
SM00179 EGF_CA, 2 hits
SM00060 FN3, 2 hits
SM00282 LamG, 3 hits
SUPFAMiSSF49265 SSF49265, 1 hit
SSF49899 SSF49899, 3 hits
PROSITEiView protein in PROSITE
PS00022 EGF_1, 3 hits
PS01186 EGF_2, 2 hits
PS50026 EGF_3, 3 hits
PS50853 FN3, 2 hits
PS50025 LAM_G_DOMAIN, 3 hits
ProtoNetiSearch...

Entry informationi

Entry nameiEGFLA_MOUSE
AccessioniPrimary (citable) accession number: Q4VBE4
Secondary accession number(s): B2RWU6
, B6JU28, Q80WX4, Q8BGP3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: July 5, 2005
Last modified: November 7, 2018
This is version 108 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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