UniProtKB - Q4V8I7 (LRC8A_RAT)
Protein
Volume-regulated anion channel subunit LRRC8A
Gene
Lrrc8a
Organism
Rattus norvegicus (Rat)
Status
Functioni
Essential component of the volume-regulated anion channel (VRAC, also named VSOAC channel), an anion channel required to maintain a constant cell volume in response to extracellular or intracellular osmotic changes (PubMed:28833202). The VRAC channel conducts iodide better than chloride and can also conduct organic osmolytes like taurine (By similarity). Mediates efflux of amino acids, such as aspartate and glutamate, in response to osmotic stress (PubMed:28833202). Required for in vivo channel activity, together with at least one other family member (LRRC8B, LRRC8C, LRRC8D or LRRC8E); channel characteristics depend on the precise subunit composition (PubMed:28833202). Can form functional channels by itself (in vitro). Involved in B-cell development: required for the pro-B cell to pre-B cell transition (By similarity). Also required for T-cell development (By similarity). May play a role in lysosome homeostasis (By similarity).By similarity1 Publication
GO - Molecular functioni
- identical protein binding Source: RGD
- volume-sensitive anion channel activity Source: UniProtKB
GO - Biological processi
- anion transmembrane transport Source: UniProtKB
- anion transport Source: UniProtKB
- aspartate transmembrane transport Source: UniProtKB
- cell volume homeostasis Source: UniProtKB
- pre-B cell differentiation Source: UniProtKB
- protein hexamerization Source: UniProtKB
- response to osmotic stress Source: UniProtKB
- taurine transport Source: UniProtKB
Keywordsi
Molecular function | Ion channel |
Biological process | Differentiation, Ion transport, Transport |
Enzyme and pathway databases
Reactomei | R-RNO-5223345, Miscellaneous transport and binding events |
Names & Taxonomyi
Protein namesi | Recommended name: Volume-regulated anion channel subunit LRRC8AAlternative name(s): Leucine-rich repeat-containing protein 8A |
Gene namesi | Name:Lrrc8a Synonyms:Lrrc8 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 1311690, Lrrc8a |
Subcellular locationi
Plasma membrane
- Cell membrane 1 Publication; Multi-pass membrane protein By similarity
Note: The leucine-rich repeat (LRR) domain is on the cytoplasmic side of the cell membrane.By similarity
Plasma Membrane
- integral component of plasma membrane Source: UniProtKB
- plasma membrane Source: UniProtKB
Other locations
- cell surface Source: RGD
- ion channel complex Source: UniProtKB
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 22 | CytoplasmicSequence analysisAdd BLAST | 22 | |
Transmembranei | 23 – 43 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 44 – 123 | ExtracellularSequence analysisAdd BLAST | 80 | |
Transmembranei | 124 – 144 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 145 – 268 | CytoplasmicSequence analysisAdd BLAST | 124 | |
Transmembranei | 269 – 289 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 290 – 323 | ExtracellularSequence analysisAdd BLAST | 34 | |
Transmembranei | 324 – 344 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 345 – 810 | CytoplasmicSequence analysisAdd BLAST | 466 |
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000084501 | 1 – 810 | Volume-regulated anion channel subunit LRRC8AAdd BLAST | 810 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineBy similarity | 1 | |
Disulfide bondi | 54 ↔ 310 | By similarity | ||
Disulfide bondi | 57 ↔ 65 | By similarity | ||
Glycosylationi | 66 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 83 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 113 ↔ 295 | By similarity | ||
Modified residuei | 200 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 202 | PhosphoserineCombined sources | 1 | |
Modified residuei | 215 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 217 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
N-glycosylated.By similarity
Keywords - PTMi
Acetylation, Disulfide bond, Glycoprotein, PhosphoproteinProteomic databases
jPOSTi | Q4V8I7 |
PaxDbi | Q4V8I7 |
PRIDEi | Q4V8I7 |
PTM databases
GlyGeni | Q4V8I7, 2 sites |
iPTMneti | Q4V8I7 |
PhosphoSitePlusi | Q4V8I7 |
Expressioni
Gene expression databases
Bgeei | ENSRNOG00000025296, Expressed in frontal cortex and 21 other tissues |
Genevisiblei | Q4V8I7, RN |
Interactioni
Subunit structurei
Hexamer. Heterohexamer; oligomerizes with other LRRC8 proteins (LRRC8B, LRRC8C, LRRC8D and/or LRRC8E) to form a heterohexamer. Can form homohexamers in vitro, but these have lower conductance than heterohexamers (By similarity). Detected in a channel complex that contains LRRC8A, LRRC8C and LRRC8E (By similarity). In vivo, the subunit composition may depend primarily on expression levels, and heterooligomeric channels containing various proportions of the different LRRC8 proteins may coexist (Probable).
By similarity1 PublicationGO - Molecular functioni
- identical protein binding Source: RGD
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000031726 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 399 – 422 | LRR 1Sequence analysisAdd BLAST | 24 | |
Repeati | 423 – 445 | LRR 2Sequence analysisAdd BLAST | 23 | |
Repeati | 447 – 468 | LRR 3Sequence analysisAdd BLAST | 22 | |
Repeati | 469 – 492 | LRR 4Sequence analysisAdd BLAST | 24 | |
Repeati | 493 – 515 | LRR 5Sequence analysisAdd BLAST | 23 | |
Repeati | 518 – 542 | LRR 6Sequence analysisAdd BLAST | 25 | |
Repeati | 543 – 565 | LRR 7Sequence analysisAdd BLAST | 23 | |
Repeati | 567 – 589 | LRR 8Sequence analysisAdd BLAST | 23 | |
Repeati | 590 – 613 | LRR 9Sequence analysisAdd BLAST | 24 | |
Repeati | 614 – 637 | LRR 10Sequence analysisAdd BLAST | 24 | |
Repeati | 639 – 661 | LRR 11Sequence analysisAdd BLAST | 23 | |
Repeati | 662 – 684 | LRR 12Sequence analysisAdd BLAST | 23 | |
Repeati | 686 – 707 | LRR 13Sequence analysisAdd BLAST | 22 | |
Repeati | 708 – 730 | LRR 14Sequence analysisAdd BLAST | 23 | |
Repeati | 732 – 753 | LRR 15Sequence analysisAdd BLAST | 22 | |
Repeati | 754 – 776 | LRR 16Sequence analysisAdd BLAST | 23 | |
Repeati | 778 – 801 | LRR 17Sequence analysisAdd BLAST | 24 |
Sequence similaritiesi
Belongs to the LRRC8 family.Curated
Keywords - Domaini
Leucine-rich repeat, Repeat, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0619, Eukaryota |
GeneTreei | ENSGT00940000154043 |
HOGENOMi | CLU_019019_0_0_1 |
InParanoidi | Q4V8I7 |
OMAi | NELFQCK |
OrthoDBi | 158390at2759 |
PhylomeDBi | Q4V8I7 |
TreeFami | TF331443 |
Family and domain databases
Gene3Di | 3.80.10.10, 2 hits |
InterProi | View protein in InterPro IPR001611, Leu-rich_rpt IPR003591, Leu-rich_rpt_typical-subtyp IPR032675, LRR_dom_sf IPR021040, LRRC8_Pannexin-like |
Pfami | View protein in Pfam PF13855, LRR_8, 3 hits PF12534, Pannexin_like, 1 hit |
SMARTi | View protein in SMART SM00369, LRR_TYP, 8 hits |
PROSITEi | View protein in PROSITE PS51450, LRR, 11 hits |
i Sequence
Sequence statusi: Complete.
Q4V8I7-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MIPVTELRYF ADTQPAYRIL KPWWDVFTDY ISIVMLMIAV FGGTLQVTQD
60 70 80 90 100
KMICLPCKWV TKDSCNDSFR GWGASSPEPT YPNSTVLPTP DTGPTGIKYD
110 120 130 140 150
LDRHQYNYVD AVCYENRLHW FAKYFPYLVL LHTLIFLACS NFWFKFPRTS
160 170 180 190 200
SKLEHFVSIL LKCFDSPWTT RALSETVVEE SDPKPAFSKM NGSMDKKSST
210 220 230 240 250
VSEDVEATVP MLQRTKSRIE QGIVDRSETG VLDKKEGEQA KALFEKVKKF
260 270 280 290 300
RTHVEEGDIV YRLYMRQTII KVIKFFLIIC YTVYYVHNIK FDVDCTVDIE
310 320 330 340 350
SLTGYRTYRC AHPLATLFKI LASFYISLVI FYGLICMYTL WWMLRRSLKK
360 370 380 390 400
YSFESIREES SYSDIPDVKN DFAFMLHLID QYDPLYSKRF AVFLSEVSEN
410 420 430 440 450
KLRQLNLNNE WTLDKLRQRL TKNAQDKLEL HLFMLSGIPD TVFDLLELEV
460 470 480 490 500
LKLELIPDVT IPPSIAQLTG LKELWLYHTA AKIEAPALAF LRENLRALHI
510 520 530 540 550
KFTDIKEIPL WIYSLKTLEE LHLTGNLSAE NNRYIVIDGL RELKRLKVLR
560 570 580 590 600
LKSNLSKLPQ VVTDVGVHLQ KLSINNEGTK LIVLNSLKKM VNLTELELIR
610 620 630 640 650
CDLERIPHSI FSLHNLQEID LKDNNLKTIE EIISFQHLHR LTCLKLWYNH
660 670 680 690 700
IAYIPIQIGN LTNLERLYLN RNKIEKIPTQ LFYCRKLRYL DLSHNNLTLL
710 720 730 740 750
PADIGLLQNL QNLAVTANRI EALPPELFQC RKLRALHLGN NVLQSLPSRV
760 770 780 790 800
GELTNLTQIE LRGNRLECLP VELGECPLLK RSGLVVEEDL FSTLPPEVKE
810
RLWRADKEQA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC097371 mRNA Translation: AAH97371.1 |
RefSeqi | NP_001019953.1, NM_001024782.1 XP_017447329.1, XM_017591840.1 XP_017447330.1, XM_017591841.1 XP_017447331.1, XM_017591842.1 XP_017447332.1, XM_017591843.1 |
Genome annotation databases
Ensembli | ENSRNOT00000033934; ENSRNOP00000031726; ENSRNOG00000025296 ENSRNOT00000078932; ENSRNOP00000071543; ENSRNOG00000025296 |
GeneIDi | 311846 |
KEGGi | rno:311846 |
UCSCi | RGD:1311690, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC097371 mRNA Translation: AAH97371.1 |
RefSeqi | NP_001019953.1, NM_001024782.1 XP_017447329.1, XM_017591840.1 XP_017447330.1, XM_017591841.1 XP_017447331.1, XM_017591842.1 XP_017447332.1, XM_017591843.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000031726 |
PTM databases
GlyGeni | Q4V8I7, 2 sites |
iPTMneti | Q4V8I7 |
PhosphoSitePlusi | Q4V8I7 |
Proteomic databases
jPOSTi | Q4V8I7 |
PaxDbi | Q4V8I7 |
PRIDEi | Q4V8I7 |
Genome annotation databases
Ensembli | ENSRNOT00000033934; ENSRNOP00000031726; ENSRNOG00000025296 ENSRNOT00000078932; ENSRNOP00000071543; ENSRNOG00000025296 |
GeneIDi | 311846 |
KEGGi | rno:311846 |
UCSCi | RGD:1311690, rat |
Organism-specific databases
CTDi | 56262 |
RGDi | 1311690, Lrrc8a |
Phylogenomic databases
eggNOGi | KOG0619, Eukaryota |
GeneTreei | ENSGT00940000154043 |
HOGENOMi | CLU_019019_0_0_1 |
InParanoidi | Q4V8I7 |
OMAi | NELFQCK |
OrthoDBi | 158390at2759 |
PhylomeDBi | Q4V8I7 |
TreeFami | TF331443 |
Enzyme and pathway databases
Reactomei | R-RNO-5223345, Miscellaneous transport and binding events |
Miscellaneous databases
PROi | PR:Q4V8I7 |
Gene expression databases
Bgeei | ENSRNOG00000025296, Expressed in frontal cortex and 21 other tissues |
Genevisiblei | Q4V8I7, RN |
Family and domain databases
Gene3Di | 3.80.10.10, 2 hits |
InterProi | View protein in InterPro IPR001611, Leu-rich_rpt IPR003591, Leu-rich_rpt_typical-subtyp IPR032675, LRR_dom_sf IPR021040, LRRC8_Pannexin-like |
Pfami | View protein in Pfam PF13855, LRR_8, 3 hits PF12534, Pannexin_like, 1 hit |
SMARTi | View protein in SMART SM00369, LRR_TYP, 8 hits |
PROSITEi | View protein in PROSITE PS51450, LRR, 11 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | LRC8A_RAT | |
Accessioni | Q4V8I7Primary (citable) accession number: Q4V8I7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 7, 2006 |
Last sequence update: | July 5, 2005 | |
Last modified: | December 2, 2020 | |
This is version 118 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families