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Protein

Volume-regulated anion channel subunit LRRC8A

Gene

Lrrc8a

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Essential component of the volume-regulated anion channel (VRAC, also named VSOAC channel), an anion channel required to maintain a constant cell volume in response to extracellular or intracellular osmotic changes (PubMed:28833202). The VRAC channel conducts iodide better than chloride and can also conduct organic osmolytes like taurine (By similarity). Mediates efflux of amino acids, such as aspartate and glutamate, in response to osmotic stress (PubMed:28833202). Required for in vivo channel activity, together with at least one other family member (LRRC8B, LRRC8C, LRRC8D or LRRC8E); channel characteristics depend on the precise subunit composition (PubMed:28833202). Can form functional channels by itself (in vitro). Involved in B-cell development: required for the pro-B cell to pre-B cell transition (By similarity). Also required for T-cell development (By similarity).By similarity1 Publication

GO - Molecular functioni

  • identical protein binding Source: Ensembl
  • volume-sensitive anion channel activity Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionIon channel
Biological processDifferentiation, Ion transport, Transport

Enzyme and pathway databases

ReactomeiR-RNO-5223345 Miscellaneous transport and binding events

Names & Taxonomyi

Protein namesi
Recommended name:
Volume-regulated anion channel subunit LRRC8A
Alternative name(s):
Leucine-rich repeat-containing protein 8A
Gene namesi
Name:Lrrc8a
Synonyms:Lrrc8
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi1311690 Lrrc8a

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 22CytoplasmicSequence analysisAdd BLAST22
Transmembranei23 – 43HelicalSequence analysisAdd BLAST21
Topological domaini44 – 123ExtracellularSequence analysisAdd BLAST80
Transmembranei124 – 144HelicalSequence analysisAdd BLAST21
Topological domaini145 – 268CytoplasmicSequence analysisAdd BLAST124
Transmembranei269 – 289HelicalSequence analysisAdd BLAST21
Topological domaini290 – 323ExtracellularSequence analysisAdd BLAST34
Transmembranei324 – 344HelicalSequence analysisAdd BLAST21
Topological domaini345 – 810CytoplasmicSequence analysisAdd BLAST466

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000845011 – 810Volume-regulated anion channel subunit LRRC8AAdd BLAST810

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Disulfide bondi54 ↔ 310By similarity
Disulfide bondi57 ↔ 65By similarity
Glycosylationi66N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi83N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi113 ↔ 295By similarity
Modified residuei200PhosphothreonineBy similarity1
Modified residuei202PhosphoserineCombined sources1
Modified residuei215PhosphothreonineBy similarity1
Modified residuei217PhosphoserineCombined sources1

Post-translational modificationi

N-glycosylated.By similarity

Keywords - PTMi

Acetylation, Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ4V8I7
PRIDEiQ4V8I7

PTM databases

iPTMnetiQ4V8I7
PhosphoSitePlusiQ4V8I7

Expressioni

Gene expression databases

BgeeiENSRNOG00000025296 Expressed in 10 organ(s), highest expression level in brain
GenevisibleiQ4V8I7 RN

Interactioni

Subunit structurei

Hexamer. Heterohexamer; oligomerizes with other LRRC8 proteins (LRRC8B, LRRC8C, LRRC8D and/or LRRC8E) to form a heterohexamer. Can form homohexamers in vitro, but these have lower conductance than heterohexamers (By similarity). Detected in a channel complex that contains LRRC8A, LRRC8C and LRRC8E (By similarity). In vivo, the subunit composition may depend primarily on expression levels, and heterooligomeric channels containing various proportions of the different LRRC8 proteins may coexist (Probable).By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000031726

Structurei

3D structure databases

ProteinModelPortaliQ4V8I7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati399 – 422LRR 1Sequence analysisAdd BLAST24
Repeati423 – 445LRR 2Sequence analysisAdd BLAST23
Repeati447 – 468LRR 3Sequence analysisAdd BLAST22
Repeati469 – 492LRR 4Sequence analysisAdd BLAST24
Repeati493 – 515LRR 5Sequence analysisAdd BLAST23
Repeati518 – 542LRR 6Sequence analysisAdd BLAST25
Repeati543 – 565LRR 7Sequence analysisAdd BLAST23
Repeati567 – 589LRR 8Sequence analysisAdd BLAST23
Repeati590 – 613LRR 9Sequence analysisAdd BLAST24
Repeati614 – 637LRR 10Sequence analysisAdd BLAST24
Repeati639 – 661LRR 11Sequence analysisAdd BLAST23
Repeati662 – 684LRR 12Sequence analysisAdd BLAST23
Repeati686 – 707LRR 13Sequence analysisAdd BLAST22
Repeati708 – 730LRR 14Sequence analysisAdd BLAST23
Repeati732 – 753LRR 15Sequence analysisAdd BLAST22
Repeati754 – 776LRR 16Sequence analysisAdd BLAST23
Repeati778 – 801LRR 17Sequence analysisAdd BLAST24

Sequence similaritiesi

Belongs to the LRRC8 family.Curated

Keywords - Domaini

Leucine-rich repeat, Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0619 Eukaryota
COG4886 LUCA
GeneTreeiENSGT00920000148937
HOGENOMiHOG000231806
HOVERGENiHBG052360
InParanoidiQ4V8I7
KOiK22038
OMAiVDTQPAY
PhylomeDBiQ4V8I7
TreeFamiTF331443

Family and domain databases

Gene3Di3.80.10.10, 2 hits
InterProiView protein in InterPro
IPR001611 Leu-rich_rpt
IPR003591 Leu-rich_rpt_typical-subtyp
IPR032675 LRR_dom_sf
IPR038960 LRRC8
IPR021040 LRRC8_Pannexin-like
PANTHERiPTHR43945 PTHR43945, 1 hit
PfamiView protein in Pfam
PF13855 LRR_8, 3 hits
PF12534 Pannexin_like, 1 hit
SMARTiView protein in SMART
SM00369 LRR_TYP, 8 hits
PROSITEiView protein in PROSITE
PS51450 LRR, 11 hits

Sequencei

Sequence statusi: Complete.

Q4V8I7-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MIPVTELRYF ADTQPAYRIL KPWWDVFTDY ISIVMLMIAV FGGTLQVTQD
60 70 80 90 100
KMICLPCKWV TKDSCNDSFR GWGASSPEPT YPNSTVLPTP DTGPTGIKYD
110 120 130 140 150
LDRHQYNYVD AVCYENRLHW FAKYFPYLVL LHTLIFLACS NFWFKFPRTS
160 170 180 190 200
SKLEHFVSIL LKCFDSPWTT RALSETVVEE SDPKPAFSKM NGSMDKKSST
210 220 230 240 250
VSEDVEATVP MLQRTKSRIE QGIVDRSETG VLDKKEGEQA KALFEKVKKF
260 270 280 290 300
RTHVEEGDIV YRLYMRQTII KVIKFFLIIC YTVYYVHNIK FDVDCTVDIE
310 320 330 340 350
SLTGYRTYRC AHPLATLFKI LASFYISLVI FYGLICMYTL WWMLRRSLKK
360 370 380 390 400
YSFESIREES SYSDIPDVKN DFAFMLHLID QYDPLYSKRF AVFLSEVSEN
410 420 430 440 450
KLRQLNLNNE WTLDKLRQRL TKNAQDKLEL HLFMLSGIPD TVFDLLELEV
460 470 480 490 500
LKLELIPDVT IPPSIAQLTG LKELWLYHTA AKIEAPALAF LRENLRALHI
510 520 530 540 550
KFTDIKEIPL WIYSLKTLEE LHLTGNLSAE NNRYIVIDGL RELKRLKVLR
560 570 580 590 600
LKSNLSKLPQ VVTDVGVHLQ KLSINNEGTK LIVLNSLKKM VNLTELELIR
610 620 630 640 650
CDLERIPHSI FSLHNLQEID LKDNNLKTIE EIISFQHLHR LTCLKLWYNH
660 670 680 690 700
IAYIPIQIGN LTNLERLYLN RNKIEKIPTQ LFYCRKLRYL DLSHNNLTLL
710 720 730 740 750
PADIGLLQNL QNLAVTANRI EALPPELFQC RKLRALHLGN NVLQSLPSRV
760 770 780 790 800
GELTNLTQIE LRGNRLECLP VELGECPLLK RSGLVVEEDL FSTLPPEVKE
810
RLWRADKEQA
Length:810
Mass (Da):94,162
Last modified:July 5, 2005 - v1
Checksum:iA05EE234C10615C0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC097371 mRNA Translation: AAH97371.1
RefSeqiNP_001019953.1, NM_001024782.1
XP_017447329.1, XM_017591840.1
XP_017447330.1, XM_017591841.1
XP_017447331.1, XM_017591842.1
XP_017447332.1, XM_017591843.1
UniGeneiRn.33512

Genome annotation databases

EnsembliENSRNOT00000033934; ENSRNOP00000031726; ENSRNOG00000025296
ENSRNOT00000078932; ENSRNOP00000071543; ENSRNOG00000025296
GeneIDi311846
KEGGirno:311846
UCSCiRGD:1311690 rat

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC097371 mRNA Translation: AAH97371.1
RefSeqiNP_001019953.1, NM_001024782.1
XP_017447329.1, XM_017591840.1
XP_017447330.1, XM_017591841.1
XP_017447331.1, XM_017591842.1
XP_017447332.1, XM_017591843.1
UniGeneiRn.33512

3D structure databases

ProteinModelPortaliQ4V8I7
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000031726

PTM databases

iPTMnetiQ4V8I7
PhosphoSitePlusiQ4V8I7

Proteomic databases

PaxDbiQ4V8I7
PRIDEiQ4V8I7

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000033934; ENSRNOP00000031726; ENSRNOG00000025296
ENSRNOT00000078932; ENSRNOP00000071543; ENSRNOG00000025296
GeneIDi311846
KEGGirno:311846
UCSCiRGD:1311690 rat

Organism-specific databases

CTDi56262
RGDi1311690 Lrrc8a

Phylogenomic databases

eggNOGiKOG0619 Eukaryota
COG4886 LUCA
GeneTreeiENSGT00920000148937
HOGENOMiHOG000231806
HOVERGENiHBG052360
InParanoidiQ4V8I7
KOiK22038
OMAiVDTQPAY
PhylomeDBiQ4V8I7
TreeFamiTF331443

Enzyme and pathway databases

ReactomeiR-RNO-5223345 Miscellaneous transport and binding events

Miscellaneous databases

PROiPR:Q4V8I7

Gene expression databases

BgeeiENSRNOG00000025296 Expressed in 10 organ(s), highest expression level in brain
GenevisibleiQ4V8I7 RN

Family and domain databases

Gene3Di3.80.10.10, 2 hits
InterProiView protein in InterPro
IPR001611 Leu-rich_rpt
IPR003591 Leu-rich_rpt_typical-subtyp
IPR032675 LRR_dom_sf
IPR038960 LRRC8
IPR021040 LRRC8_Pannexin-like
PANTHERiPTHR43945 PTHR43945, 1 hit
PfamiView protein in Pfam
PF13855 LRR_8, 3 hits
PF12534 Pannexin_like, 1 hit
SMARTiView protein in SMART
SM00369 LRR_TYP, 8 hits
PROSITEiView protein in PROSITE
PS51450 LRR, 11 hits
ProtoNetiSearch...

Entry informationi

Entry nameiLRC8A_RAT
AccessioniPrimary (citable) accession number: Q4V8I7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: July 5, 2005
Last modified: September 12, 2018
This is version 106 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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