UniProtKB - Q4V328 (GRAP1_HUMAN)
GRIP1-associated protein 1
GRIPAP1
Functioni
Regulates the endosomal recycling back to the neuronal plasma membrane, possibly by connecting early and late recycling endosomal domains and promoting segregation of recycling endosomes from early endosomal membranes. Involved in the localization of recycling endosomes to dendritic spines, thereby playing a role in the maintenance of dendritic spine morphology. Required for the activity-induced AMPA receptor recycling to dendrite membranes and for long-term potentiation and synaptic plasticity (By similarity).
By similarityFunctions as a scaffold protein to facilitate MAP3K1/MEKK1-mediated activation of the JNK1 kinase by phosphorylation, possibly by bringing MAP3K1/MEKK1 and JNK1 in close proximity.
1 PublicationMiscellaneous
GO - Molecular functioni
- identical protein binding Source: IntAct
GO - Biological processi
- neurotransmitter receptor transport, endosome to postsynaptic membrane Source: GO_Central
- regulation of modification of synaptic structure Source: GO_Central
- regulation of neurotransmitter receptor transport, endosome to postsynaptic membrane Source: GO_Central
- regulation of recycling endosome localization within postsynapse Source: GO_Central
Keywordsi
Biological process | Protein transport, Transport |
Enzyme and pathway databases
PathwayCommonsi | Q4V328 |
SignaLinki | Q4V328 |
SIGNORi | Q4V328 |
Names & Taxonomyi
Protein namesi | Recommended name: GRIP1-associated protein 1CuratedShort name: GRASP-1 Cleaved into the following chain: GRASP-1 C-terminal chainBy similarity Alternative name(s): 30kDa C-terminus form |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:18706, GRIPAP1 |
MIMi | 300408, gene |
neXtProti | NX_Q4V328 |
VEuPathDBi | HostDB:ENSG00000068400 |
Subcellular locationi
Endosome
- Early endosome membrane 1 Publication; Peripheral membrane protein Curated
- Recycling endosome membrane By similarity; Peripheral membrane protein By similarity
Other locations
Note: Localizes to recycling endosomal tubules that are emanating from early endosomes.By similarity
Cytosol
- cytosol Source: HPA
Endosome
- extrinsic component of postsynaptic early endosome membrane Source: GO_Central
- postsynaptic recycling endosome Source: GO_Central
- recycling endosome membrane Source: UniProtKB-SubCell
Extracellular region or secreted
- blood microparticle Source: UniProtKB
Nucleus
- nucleoplasm Source: HPA
Other locations
- axon Source: UniProtKB-SubCell
- dendrite Source: UniProtKB-SubCell
- glutamatergic synapse Source: GO_Central
- intracellular membrane-bounded organelle Source: HPA
Keywords - Cellular componenti
Cell junction, Cell projection, Endosome, Membrane, SynapsePathology & Biotechi
Organism-specific databases
OpenTargetsi | ENSG00000068400 |
PharmGKBi | PA38650 |
Miscellaneous databases
Pharosi | Q4V328, Tbio |
Genetic variation databases
BioMutai | GRIPAP1 |
DMDMi | 74753569 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | |||
ChainiPRO_0000087581 | 2 – 841 | GRIP1-associated protein 1Add BLAST | 840 | |
ChainiPRO_0000441811 | 599 – 841 | GRASP-1 C-terminal chainAdd BLAST | 243 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineCombined sources | 1 | |
Modified residuei | 655 | PhosphoserineCombined sources | 1 | |
Modified residuei | 666 | PhosphoserineCombined sources | 1 | |
Modified residuei | 668 | PhosphoserineBy similarity | 1 | |
Modified residuei | 669 | PhosphoserineBy similarity | 1 | |
Modified residuei | 688 | PhosphoserineBy similarity | 1 | |
Modified residuei | 690 | PhosphoserineCombined sources | 1 | |
Modified residuei | 691 | PhosphoserineBy similarity | 1 | |
Modified residuei | 692 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 598 – 599 | Cleavage; by caspase-3By similarity | 2 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | Q4V328 |
jPOSTi | Q4V328 |
MassIVEi | Q4V328 |
PaxDbi | Q4V328 |
PeptideAtlasi | Q4V328 |
PRIDEi | Q4V328 |
ProteomicsDBi | 62272 [Q4V328-1] 62273 [Q4V328-2] 62274 [Q4V328-3] 62275 [Q4V328-4] |
PTM databases
GlyGeni | Q4V328, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | Q4V328 |
MetOSitei | Q4V328 |
PhosphoSitePlusi | Q4V328 |
Expressioni
Gene expression databases
Bgeei | ENSG00000068400, Expressed in frontal cortex and 212 other tissues |
ExpressionAtlasi | Q4V328, baseline and differential |
Genevisiblei | Q4V328, HS |
Organism-specific databases
HPAi | ENSG00000068400, Low tissue specificity |
Interactioni
Subunit structurei
Interacts with GRIP1, GRIP2 and AMPA receptors (By similarity).
Interacts (via C-terminus) with MAPK8/JNK1 and MAP3K1/MEKK1; the interaction promotes MAP3K1-mediated phosphorylation of MAPK8 (PubMed:17761173).
Interacts (via N-terminus) with RAB4A (in GTP-bound form) (PubMed:20098723).
Interacts (via C-terminus) with STX12 (By similarity).
By similarity2 PublicationsBinary interactionsi
Q4V328
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 121210, 150 interactors |
CORUMi | Q4V328 |
DIPi | DIP-47299N |
IntActi | Q4V328, 77 interactors |
MINTi | Q4V328 |
STRINGi | 9606.ENSP00000365606 |
Miscellaneous databases
RNActi | Q4V328, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 532 – 551 | DisorderedSequence analysisAdd BLAST | 20 | |
Regioni | 558 – 580 | DisorderedSequence analysisAdd BLAST | 23 | |
Regioni | 681 – 706 | DisorderedSequence analysisAdd BLAST | 26 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 4 – 161 | Sequence analysisAdd BLAST | 158 | |
Coiled coili | 208 – 641 | Sequence analysisAdd BLAST | 434 | |
Coiled coili | 701 – 735 | Sequence analysisAdd BLAST | 35 | |
Coiled coili | 785 – 814 | Sequence analysisAdd BLAST | 30 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 684 – 698 | Polar residuesSequence analysisAdd BLAST | 15 |
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | ENOG502QTUD, Eukaryota |
GeneTreei | ENSGT00720000108868 |
HOGENOMi | CLU_019728_1_0_1 |
InParanoidi | Q4V328 |
OMAi | FLLVQEQ |
OrthoDBi | 1273136at2759 |
PhylomeDBi | Q4V328 |
TreeFami | TF329006 |
Family and domain databases
InterProi | View protein in InterPro IPR026204, GRIPAP1 |
PANTHERi | PTHR18978, PTHR18978, 2 hits |
s (4+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 4 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 4 described isoforms and 6 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAQALSEEEF QRMQAQLLEL RTNNYQLSDE LRKNGVELTS LRQKVAYLDK
60 70 80 90 100
EFSKAQKALS KSKKAQEVEV LLSENEMLQA KLHSQEEDFR LQNSTLMAEF
110 120 130 140 150
SKLCSQMEQL EQENQQLKEG AAGAGVAQAG PLVDGELLRL QAENTALQKN
160 170 180 190 200
VAALQERYGK EAGKFSAVSE GQGDPPGGLA PTVLAPMPLA EVELKWEMEK
210 220 230 240 250
EEKRLLWEQL QGLESSKQAE TSRLQEELAK LSEKLKKKQE SFCRLQTEKE
260 270 280 290 300
TLFNDSRNKI EELQQRKEAD HKAQLARTQK LQQELEAANQ SLAELRDQRQ
310 320 330 340 350
GERLEHAAAL RALQDQVSIQ SADAQEQVEG LLAENNALRT SLAALEQIQT
360 370 380 390 400
AKTQELNMLR EQTTGLAAEL QQQQAEYEDL MGQKDDLNSQ LQESLRANSR
410 420 430 440 450
LLEQLQEIGQ EKEQLTQELQ EARKSAEKRK AMLDELAMET LQEKSQHKEE
460 470 480 490 500
LGAVRLRHEK EVLGVRARYE RELRELHEDK KRQEEELRGQ IREEKARTRE
510 520 530 540 550
LETLQQTVEE LQAQVHSMDG AKGWFERRLK EAEESLQQQQ QEQEEALKQC
560 570 580 590 600
REQHAAELKG KEEELQDVRD QLEQAQEERD CHLKTISSLK QEVKDTVDGQ
610 620 630 640 650
RILEKKGSAA LKDLKRQLHL ERKRADKLQE RLQDILTNSK SRSGLEELVL
660 670 680 690 700
SEMNSPSRTQ TGDSSSISSF SYREILREKE SSAVPARSLS SSPQAQPPRP
710 720 730 740 750
AELSDEEVAE LFQRLAETQQ EKWMLEEKVK HLEVSSASMA EDLCRKSAII
760 770 780 790 800
ETYVMDSRID VSVAAGHTDR SGLGSVLRDL VKPGDENLRE MNKKLQNMLE
810 820 830 840
EQLTKNMHLH KDMEVLSQEI VRLSKECVGP PDPDLEPGET S
Computationally mapped potential isoform sequencesi
There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A087WT45 | A0A087WT45_HUMAN | GRIP1-associated protein 1 | GRIPAP1 | 796 | Annotation score: | ||
A0A087WXA6 | A0A087WXA6_HUMAN | GRIP1-associated protein 1 | GRIPAP1 | 302 | Annotation score: | ||
A0A087WZF5 | A0A087WZF5_HUMAN | GRIP1-associated protein 1 | GRIPAP1 | 121 | Annotation score: | ||
A0A087WW92 | A0A087WW92_HUMAN | GRIP1-associated protein 1 | GRIPAP1 | 69 | Annotation score: | ||
A0A087WV84 | A0A087WV84_HUMAN | GRIP1-associated protein 1 | GRIPAP1 | 23 | Annotation score: | ||
A0A087WWF2 | A0A087WWF2_HUMAN | GRIP1-associated protein 1 | GRIPAP1 | 61 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 677 | R → G in CAB95949 (PubMed:10908365).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_080637 | 179 | L → P3 PublicationsCorresponds to variant dbSNP:rs61735977Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_015699 | 1 – 110 | Missing in isoform 3. 1 PublicationAdd BLAST | 110 | |
Alternative sequenceiVSP_015700 | 103 – 155 | Missing in isoform 2. 1 PublicationAdd BLAST | 53 | |
Alternative sequenceiVSP_015701 | 111 – 152 | EQENQ…QKNVA → MWSFGTGTLPPEGLKASSPS IRGMARDFRDHLIHHIFLFY SP in isoform 3. 1 PublicationAdd BLAST | 42 | |
Alternative sequenceiVSP_015702 | 317 – 347 | Missing in isoform 4. CuratedAdd BLAST | 31 | |
Alternative sequenceiVSP_015703 | 534 – 559 | Missing in isoform 2. 1 PublicationAdd BLAST | 26 | |
Alternative sequenceiVSP_015704 | 688 – 704 | SLSSS…PAELS → VRGKEEPTAPASLNPKI in isoform 2 and isoform 3. 1 PublicationAdd BLAST | 17 | |
Alternative sequenceiVSP_015705 | 705 – 841 | Missing in isoform 2 and isoform 3. 1 PublicationAdd BLAST | 137 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BX640704 mRNA Translation: CAE45824.1 BX647804 mRNA Translation: CAI45985.1 Different termination. AF207550 Genomic DNA No translation available. BX530088 Genomic DNA No translation available. BC001522 mRNA Translation: AAH01522.2 BC101544 mRNA Translation: AAI01545.1 BC101546 mRNA Translation: AAI01547.1 AB032993 mRNA Translation: BAA86481.1 AJ297364 mRNA Translation: CAB95949.1 |
CCDSi | CCDS35248.1 [Q4V328-1] |
RefSeqi | NP_064522.3, NM_020137.4 [Q4V328-1] |
Genome annotation databases
Ensembli | ENST00000376423; ENSP00000365606; ENSG00000068400 ENST00000593475; ENSP00000469842; ENSG00000068400 [Q4V328-4] |
GeneIDi | 56850 |
KEGGi | hsa:56850 |
MANE-Selecti | ENST00000376423.8; ENSP00000365606.5; NM_020137.5; NP_064522.4 |
UCSCi | uc033ecr.2, human [Q4V328-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BX640704 mRNA Translation: CAE45824.1 BX647804 mRNA Translation: CAI45985.1 Different termination. AF207550 Genomic DNA No translation available. BX530088 Genomic DNA No translation available. BC001522 mRNA Translation: AAH01522.2 BC101544 mRNA Translation: AAI01545.1 BC101546 mRNA Translation: AAI01547.1 AB032993 mRNA Translation: BAA86481.1 AJ297364 mRNA Translation: CAB95949.1 |
CCDSi | CCDS35248.1 [Q4V328-1] |
RefSeqi | NP_064522.3, NM_020137.4 [Q4V328-1] |
3D structure databases
SMRi | Q4V328 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 121210, 150 interactors |
CORUMi | Q4V328 |
DIPi | DIP-47299N |
IntActi | Q4V328, 77 interactors |
MINTi | Q4V328 |
STRINGi | 9606.ENSP00000365606 |
PTM databases
GlyGeni | Q4V328, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | Q4V328 |
MetOSitei | Q4V328 |
PhosphoSitePlusi | Q4V328 |
Genetic variation databases
BioMutai | GRIPAP1 |
DMDMi | 74753569 |
Proteomic databases
EPDi | Q4V328 |
jPOSTi | Q4V328 |
MassIVEi | Q4V328 |
PaxDbi | Q4V328 |
PeptideAtlasi | Q4V328 |
PRIDEi | Q4V328 |
ProteomicsDBi | 62272 [Q4V328-1] 62273 [Q4V328-2] 62274 [Q4V328-3] 62275 [Q4V328-4] |
Protocols and materials databases
Antibodypediai | 355, 221 antibodies from 32 providers |
DNASUi | 56850 |
Genome annotation databases
Ensembli | ENST00000376423; ENSP00000365606; ENSG00000068400 ENST00000593475; ENSP00000469842; ENSG00000068400 [Q4V328-4] |
GeneIDi | 56850 |
KEGGi | hsa:56850 |
MANE-Selecti | ENST00000376423.8; ENSP00000365606.5; NM_020137.5; NP_064522.4 |
UCSCi | uc033ecr.2, human [Q4V328-1] |
Organism-specific databases
CTDi | 56850 |
GeneCardsi | GRIPAP1 |
HGNCi | HGNC:18706, GRIPAP1 |
HPAi | ENSG00000068400, Low tissue specificity |
MIMi | 300408, gene |
neXtProti | NX_Q4V328 |
OpenTargetsi | ENSG00000068400 |
PharmGKBi | PA38650 |
VEuPathDBi | HostDB:ENSG00000068400 |
HUGEi | Search... |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502QTUD, Eukaryota |
GeneTreei | ENSGT00720000108868 |
HOGENOMi | CLU_019728_1_0_1 |
InParanoidi | Q4V328 |
OMAi | FLLVQEQ |
OrthoDBi | 1273136at2759 |
PhylomeDBi | Q4V328 |
TreeFami | TF329006 |
Enzyme and pathway databases
PathwayCommonsi | Q4V328 |
SignaLinki | Q4V328 |
SIGNORi | Q4V328 |
Miscellaneous databases
BioGRID-ORCSi | 56850, 9 hits in 680 CRISPR screens |
ChiTaRSi | GRIPAP1, human |
GeneWikii | GRIPAP1 |
GenomeRNAii | 56850 |
Pharosi | Q4V328, Tbio |
PROi | PR:Q4V328 |
RNActi | Q4V328, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000068400, Expressed in frontal cortex and 212 other tissues |
ExpressionAtlasi | Q4V328, baseline and differential |
Genevisiblei | Q4V328, HS |
Family and domain databases
InterProi | View protein in InterPro IPR026204, GRIPAP1 |
PANTHERi | PTHR18978, PTHR18978, 2 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | GRAP1_HUMAN | |
Accessioni | Q4V328Primary (citable) accession number: Q4V328 Secondary accession number(s): A6NL78 Q9ULQ3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 27, 2005 |
Last sequence update: | September 12, 2018 | |
Last modified: | February 23, 2022 | |
This is version 142 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- Human chromosome X
Human chromosome X: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot