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Protein

GRIP1-associated protein 1

Gene

GRIPAP1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Regulates the endosomal recycling back to the neuronal plasma membrane, possibly by connecting early and late recycling endosomal domains and promoting segregation of recycling endosomes from early endosomal membranes. Involved in the localization of recycling endosomes to dendritic spines, thereby playing a role in the maintenance of dendritic spine morphology. Required for the activity-induced AMPA receptor recycling to dendrite membranes and for long-term potentiation and synaptic plasticity (By similarity).By similarity
GRASP-1 C-terminal chain: Functions as a scaffold protein to facilitate MAP3K1/MEKK1-mediated activation of the JNK1 kinase by phosphorylation, possibly by bringing MAP3K1/MEKK1 and JNK1 in close proximity.1 Publication

Miscellaneous

Antibodies against GRIPAP1 have been found in sera of a patient who developed Raynaud's syndrome and telangiectasias.

GO - Biological processi

Keywordsi

Biological processProtein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
GRIP1-associated protein 1
Short name:
GRASP-1
Cleaved into the following chain:
GRASP-1 C-terminal chainBy similarity
Alternative name(s):
30kDa C-terminus form
Gene namesi
Name:GRIPAP1
Synonyms:KIAA1167
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome X

Organism-specific databases

EuPathDBiHostDB:ENSG00000068400.13
HGNCiHGNC:18706 GRIPAP1
MIMi300408 gene
neXtProtiNX_Q4V328

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cell projection, Endosome, Membrane, Synapse

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA38650

Polymorphism and mutation databases

DMDMi74753569

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00000875812 – 841GRIP1-associated protein 1Add BLAST840
ChainiPRO_0000441811599 – 841GRASP-1 C-terminal chainAdd BLAST243

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Modified residuei655PhosphoserineCombined sources1
Modified residuei666PhosphoserineCombined sources1
Modified residuei668PhosphoserineBy similarity1
Modified residuei669PhosphoserineBy similarity1
Modified residuei688PhosphoserineBy similarity1
Modified residuei690PhosphoserineCombined sources1
Modified residuei691PhosphoserineBy similarity1
Modified residuei692PhosphoserineCombined sources1

Post-translational modificationi

Proteolytically cleaved by caspase-3. A minor C-terminal proteolytic fragment of 30 kDa is produced. Proteolytic cleavage is required for JNK signaling activation.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei598 – 599Cleavage; by caspase-3By similarity2

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ4V328
MaxQBiQ4V328
PaxDbiQ4V328
PeptideAtlasiQ4V328
PRIDEiQ4V328
ProteomicsDBi62272
62273 [Q4V328-2]
62274 [Q4V328-3]
62275 [Q4V328-4]

PTM databases

iPTMnetiQ4V328
PhosphoSitePlusiQ4V328

Miscellaneous databases

PMAP-CutDBiQ4V328

Expressioni

Gene expression databases

BgeeiENSG00000068400
ExpressionAtlasiQ4V328 baseline and differential
GenevisibleiQ4V328 HS

Organism-specific databases

HPAiHPA000282
HPA000615

Interactioni

Subunit structurei

Interacts with GRIP1, GRIP2 and AMPA receptors (By similarity). Interacts (via C-terminus) with MAPK8/JNK1 and MAP3K1/MEKK1; the interaction promotes MAP3K1-mediated phosphorylation of MAPK8 (PubMed:17761173). Interacts (via N-terminus) with RAB4A (in GTP-bound form) (PubMed:20098723). Interacts (via C-terminus) with STX12 (By similarity).By similarity2 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi121210, 51 interactors
CORUMiQ4V328
DIPiDIP-47299N
IntActiQ4V328, 97 interactors
STRINGi9606.ENSP00000365624

Structurei

3D structure databases

ProteinModelPortaliQ4V328
SMRiQ4V328
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili4 – 161Sequence analysisAdd BLAST158
Coiled coili208 – 641Sequence analysisAdd BLAST434
Coiled coili701 – 735Sequence analysisAdd BLAST35
Coiled coili785 – 814Sequence analysisAdd BLAST30

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IKP9 Eukaryota
ENOG410YB89 LUCA
HOVERGENiHBG080243
InParanoidiQ4V328
OrthoDBiEOG091G0AQ8
PhylomeDBiQ4V328
TreeFamiTF329006

Family and domain databases

InterProiView protein in InterPro
IPR026204 GRIPAP1
PANTHERiPTHR18978 PTHR18978, 1 hit

Sequences (4)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q4V328-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAQALSEEEF QRMQAQLLEL RTNNYQLSDE LRKNGVELTS LRQKVAYLDK
60 70 80 90 100
EFSKAQKALS KSKKAQEVEV LLSENEMLQA KLHSQEEDFR LQNSTLMAEF
110 120 130 140 150
SKLCSQMEQL EQENQQLKEG AAGAGVAQAG PLVDGELLRL QAENTALQKN
160 170 180 190 200
VAALQERYGK EAGKFSAVSE GQGDPPGGPA PTVLAPMPLA EVELKWEMEK
210 220 230 240 250
EEKRLLWEQL QGLESSKQAE TSRLQEELAK LSEKLKKKQE SFCRLQTEKE
260 270 280 290 300
TLFNDSRNKI EELQQRKEAD HKAQLARTQK LQQELEAANQ SLAELRDQRQ
310 320 330 340 350
GERLEHAAAL RALQDQVSIQ SADAQEQVEG LLAENNALRT SLAALEQIQT
360 370 380 390 400
AKTQELNMLR EQTTGLAAEL QQQQAEYEDL MGQKDDLNSQ LQESLRANSR
410 420 430 440 450
LLEQLQEIGQ EKEQLTQELQ EARKSAEKRK AMLDELAMET LQEKSQHKEE
460 470 480 490 500
LGAVRLRHEK EVLGVRARYE RELRELHEDK KRQEEELRGQ IREEKARTRE
510 520 530 540 550
LETLQQTVEE LQAQVHSMDG AKGWFERRLK EAEESLQQQQ QEQEEALKQC
560 570 580 590 600
REQHAAELKG KEEELQDVRD QLEQAQEERD CHLKTISSLK QEVKDTVDGQ
610 620 630 640 650
RILEKKGSAA LKDLKRQLHL ERKRADKLQE RLQDILTNSK SRSGLEELVL
660 670 680 690 700
SEMNSPSRTQ TGDSSSISSF SYREILREKE SSAVPARSLS SSPQAQPPRP
710 720 730 740 750
AELSDEEVAE LFQRLAETQQ EKWMLEEKVK HLEVSSASMA EDLCRKSAII
760 770 780 790 800
ETYVMDSRID VSVAAGHTDR SGLGSVLRDL VKPGDENLRE MNKKLQNMLE
810 820 830 840
EQLTKNMHLH KDMEVLSQEI VRLSKECVGP PDPDLEPGET S
Length:841
Mass (Da):95,990
Last modified:July 5, 2005 - v1
Checksum:i48877D04F6630213
GO
Isoform 2 (identifier: Q4V328-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     103-155: Missing.
     534-559: Missing.
     688-704: SLSSSPQAQPPRPAELS → VRGKEEPTAPASLNPKI
     705-841: Missing.

Note: No experimental confirmation available.
Show »
Length:625
Mass (Da):71,878
Checksum:iC79355243210C480
GO
Isoform 3 (identifier: Q4V328-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-110: Missing.
     111-152: EQENQQLKEG...ENTALQKNVA → MWSFGTGTLP...IHHIFLFYSP
     688-704: SLSSSPQAQPPRPAELS → VRGKEEPTAPASLNPKI
     705-841: Missing.

Note: No experimental confirmation available.
Show »
Length:594
Mass (Da):68,188
Checksum:i51E9C3CE7EA5B184
GO
Isoform 4 (identifier: Q4V328-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     317-347: Missing.

Note: No experimental confirmation available.
Show »
Length:810
Mass (Da):92,738
Checksum:iBC469E1FADB93F74
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti677R → G in CAB95949 (PubMed:10908365).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0156991 – 110Missing in isoform 3. 1 PublicationAdd BLAST110
Alternative sequenceiVSP_015700103 – 155Missing in isoform 2. 1 PublicationAdd BLAST53
Alternative sequenceiVSP_015701111 – 152EQENQ…QKNVA → MWSFGTGTLPPEGLKASSPS IRGMARDFRDHLIHHIFLFY SP in isoform 3. 1 PublicationAdd BLAST42
Alternative sequenceiVSP_015702317 – 347Missing in isoform 4. CuratedAdd BLAST31
Alternative sequenceiVSP_015703534 – 559Missing in isoform 2. 1 PublicationAdd BLAST26
Alternative sequenceiVSP_015704688 – 704SLSSS…PAELS → VRGKEEPTAPASLNPKI in isoform 2 and isoform 3. 1 PublicationAdd BLAST17
Alternative sequenceiVSP_015705705 – 841Missing in isoform 2 and isoform 3. 1 PublicationAdd BLAST137

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX640704 mRNA Translation: CAE45824.1
BX647804 mRNA Translation: CAI45985.1 Different termination.
AF207550 Genomic DNA No translation available.
BX530088 Genomic DNA No translation available.
BC001522 mRNA Translation: AAH01522.2
BC101544 mRNA Translation: AAI01545.1
BC101546 mRNA Translation: AAI01547.1
AB032993 mRNA Translation: BAA86481.1
AJ297364 mRNA Translation: CAB95949.1
CCDSiCCDS35248.1 [Q4V328-1]
RefSeqiNP_064522.3, NM_020137.4 [Q4V328-1]
UniGeneiHs.109929

Genome annotation databases

EnsembliENST00000376423; ENSP00000365606; ENSG00000068400
GeneIDi56850
KEGGihsa:56850
UCSCiuc033ecr.2 human [Q4V328-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiGRAP1_HUMAN
AccessioniPrimary (citable) accession number: Q4V328
Secondary accession number(s): A6NL78
, Q3MJ75, Q4V327, Q4V330, Q5HYG1, Q6N046, Q96DH8, Q9NQ43, Q9ULQ3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 27, 2005
Last sequence update: July 5, 2005
Last modified: July 18, 2018
This is version 116 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome X
    Human chromosome X: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

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