UniProtKB - Q4U2V3 (CBPC1_DANRE)
Cytosolic carboxypeptidase 1
agtpbp1
Functioni
Metallocarboxypeptidase that mediates protein deglutamylation of tubulin and non-tubulin target proteins. Catalyzes the removal of polyglutamate side chains present on the gamma-carboxyl group of glutamate residues within the C-terminal tail of alpha- and beta-tubulin. Specifically cleaves tubulin long-side-chains, while it is not able to remove the branching point glutamate. Also catalyzes the removal of polyglutamate residues from the carboxy-terminus of alpha-tubulin as well as non-tubulin proteins.
By similarityCatalytic activityi
- (L-glutamyl)(n+1)-γ-L-glutamyl-L-glutamyl-[protein] + H2O = (L-glutamyl)(n)-γ-L-glutamyl-L-glutamyl-[protein] + L-glutamateBy similarityThis reaction proceeds in the forwardBy similarity direction.
- C-terminal L-α-aminoacyl-L-glutamyl-L-glutamyl-[tubulin] + H2O = C-terminal L-α-aminoacyl-L-glutamyl-[tubulin] + L-glutamateBy similarityEC:3.4.17.24By similarityThis reaction proceeds in the forwardBy similarity direction.
Cofactori
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 845 | ZincBy similarity | 1 | |
Metal bindingi | 848 | ZincBy similarity | 1 | |
Active sitei | 895 | NucleophileBy similarity | 1 | |
Metal bindingi | 942 | ZincBy similarity | 1 |
GO - Molecular functioni
- metallocarboxypeptidase activity Source: UniProtKB
- tubulin binding Source: UniProtKB
- zinc ion binding Source: InterPro
GO - Biological processi
- cerebellar Purkinje cell differentiation Source: UniProtKB
- chordate embryonic development Source: ZFIN
- C-terminal protein deglutamylation Source: UniProtKB
- eye photoreceptor cell differentiation Source: UniProtKB
- mitochondrion organization Source: UniProtKB
- neuromuscular process Source: UniProtKB
- olfactory bulb development Source: UniProtKB
- protein side chain deglutamylation Source: UniProtKB
- T cell differentiation in thymus Source: ZFIN
Keywordsi
Molecular function | Carboxypeptidase, Hydrolase, Metalloprotease, Protease |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
BRENDAi | 3.4.17.24, 928 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:agtpbp1 Synonyms:ccp1 |
Organismi | Danio rerio (Zebrafish) (Brachydanio rerio) |
Taxonomic identifieri | 7955 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Danionidae › Danioninae › Danio |
Proteomesi |
|
Organism-specific databases
ZFINi | ZDB-GENE-081104-267, agtpbp1 |
Subcellular locationi
Mitochondrion
- Mitochondrion By similarity
Cytoplasm and Cytosol
Nucleus
- Nucleus By similarity
Cytosol
- cytosol Source: UniProtKB
Mitochondrion
- mitochondrion Source: UniProtKB
Nucleus
- nucleus Source: UniProtKB
Other locations
- cytoplasm Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, Mitochondrion, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000308692 | 1 – 1153 | Cytosolic carboxypeptidase 1Add BLAST | 1153 |
Proteomic databases
PaxDbi | Q4U2V3 |
PRIDEi | Q4U2V3 |
Interactioni
GO - Molecular functioni
- tubulin binding Source: UniProtKB
Protein-protein interaction databases
STRINGi | 7955.ENSDARP00000025120 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 357 – 400 | DisorderedSequence analysisAdd BLAST | 44 | |
Regioni | 1108 – 1153 | DisorderedSequence analysisAdd BLAST | 46 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 364 – 385 | Acidic residuesSequence analysisAdd BLAST | 22 | |
Compositional biasi | 386 – 400 | Basic and acidic residuesSequence analysisAdd BLAST | 15 | |
Compositional biasi | 1108 – 1127 | Acidic residuesSequence analysisAdd BLAST | 20 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG3641, Eukaryota |
InParanoidi | Q4U2V3 |
Family and domain databases
CDDi | cd06906, M14_Nna1, 1 hit |
Gene3Di | 1.25.10.10, 1 hit |
InterProi | View protein in InterPro IPR011989, ARM-like IPR016024, ARM-type_fold IPR033852, CBPC1/4 IPR040626, Pepdidase_M14_N IPR000834, Peptidase_M14 |
Pfami | View protein in Pfam PF18027, Pepdidase_M14_N, 1 hit PF00246, Peptidase_M14, 1 hit |
SUPFAMi | SSF48371, SSF48371, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MNKPKMAAEK SASRVLMLLS QLERLNEESR VSDNDAIRQV TGKILHLIQT
60 70 80 90 100
QEKTRKEVGS KGSGGMEIIL SSLENTRDLQ TTLNILGILN ELLTVGGGRR
110 120 130 140 150
TGLFVSKGGT AILLQLLVSS SKDPPANEEL MLHIHSLLVK VGPKDRKFGV
160 170 180 190 200
KARLNGALNV TLNLAKHNLQ NYKLLLPCLQ VLRVYSSNSV NAVSLGKSGA
210 220 230 240 250
LEILFEIVGP FSKKSTTLLK VALDTLAALL KSGMNARRAV DRGYLPTLLA
260 270 280 290 300
IYQDWHRNDT RHRHVIIRKS ILGCIKNITN IKLGRKAFIE ASGMRILYNT
310 320 330 340 350
STECLPVRTL DPLVNTSSLI MRKCFPKNRL PLPTIKSVFL YPLPHIPAGG
360 370 380 390 400
PVAQLYNQPP GVDDVVDESD ENEATEVDTE NDTENEEDDT GHKTQNDDIE
410 420 430 440 450
TDINKLRPKQ MNTRPFEELR VYEHFCREFT ETFQDIDFED SISAIPPSRV
460 470 480 490 500
NSELHRPIII PTTQSSPGLQ NRRPLRESAL PLKPEQSPLE LDSISIAKRP
510 520 530 540 550
DGRADADLVC SLGHLILDAA VNGGSVDGCQ DDGGEQSVLE VPDTAALLPL
560 570 580 590 600
HDPELYLEMV KSTRSVPGYT EVAYPDYFGH VALNLREPIL ERVYGVQRTK
610 620 630 640 650
IFQDIERLIH SSDILDKVVY DLDNQSSPLT DNGESLKFNS KFESGNLRKA
660 670 680 690 700
IQVRKFEYDL ILNSDINSNH YHQWFYFEVG NMRPGVRYRF NIINCEKSNS
710 720 730 740 750
QFNYGMQVIM YSVQEAINGS PHWVRTGSDI CYYKNHFARS SIAAGGQKGK
760 770 780 790 800
SYFTMTFTVT FQHKDDVCYF AYHYPYTYSM LKMHLQKLSA LCTPEIYYRQ
810 820 830 840 850
EDLCETLGGN GCPLLTITAM PESSSDEHIS QFRSRPVIFL SARVHPGETN
860 870 880 890 900
SSWVMKGSLE FLMSCSPQAQ SLRQSYIFKI MPMLNPDGVI NGNHRCSLSG
910 920 930 940 950
EDLNRQWQNP NAELHPTIYH AKSLLQYLRA TGRTPLVFCD YHGHSRKKNV
960 970 980 990 1000
FMYGCSIKET MWQSSVNTST CDLNEDLGYR TLPKLLSQMA PAFSLSSCSF
1010 1020 1030 1040 1050
VVERSKEATA RVVVWREIGV QRSYTMESTL CGCDQGKYKG LQIGTSELEE
1060 1070 1080 1090 1100
MGSQFCLALL RLRRFTSPLE LHNHNSHLLD MENELIDTRH IPNITSPTTY
1110 1120 1130 1140 1150
VLDEDEPAFL EEVDYSAESN DENDPELEPD LRDNHALPDP SSDSELSHQD
SLT
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A0R4IG37 | A0A0R4IG37_DANRE | Tubulin-glutamate carboxypeptidase | agtpbp1 | 1,153 | Annotation score: | ||
A0A0R4IRP2 | A0A0R4IRP2_DANRE | Cytosolic carboxypeptidase 1 | agtpbp1 | 722 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | DQ018111 mRNA Translation: AAY42353.1 |
RefSeqi | NP_001019616.1, NM_001024445.2 |
Genome annotation databases
GeneIDi | 554164 |
KEGGi | dre:554164 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | DQ018111 mRNA Translation: AAY42353.1 |
RefSeqi | NP_001019616.1, NM_001024445.2 |
3D structure databases
SMRi | Q4U2V3 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 7955.ENSDARP00000025120 |
Proteomic databases
PaxDbi | Q4U2V3 |
PRIDEi | Q4U2V3 |
Genome annotation databases
GeneIDi | 554164 |
KEGGi | dre:554164 |
Organism-specific databases
CTDi | 23287 |
ZFINi | ZDB-GENE-081104-267, agtpbp1 |
Phylogenomic databases
eggNOGi | KOG3641, Eukaryota |
InParanoidi | Q4U2V3 |
Enzyme and pathway databases
BRENDAi | 3.4.17.24, 928 |
Miscellaneous databases
PROi | PR:Q4U2V3 |
Family and domain databases
CDDi | cd06906, M14_Nna1, 1 hit |
Gene3Di | 1.25.10.10, 1 hit |
InterProi | View protein in InterPro IPR011989, ARM-like IPR016024, ARM-type_fold IPR033852, CBPC1/4 IPR040626, Pepdidase_M14_N IPR000834, Peptidase_M14 |
Pfami | View protein in Pfam PF18027, Pepdidase_M14_N, 1 hit PF00246, Peptidase_M14, 1 hit |
SUPFAMi | SSF48371, SSF48371, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CBPC1_DANRE | |
Accessioni | Q4U2V3Primary (citable) accession number: Q4U2V3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 23, 2007 |
Last sequence update: | July 5, 2005 | |
Last modified: | February 23, 2022 | |
This is version 82 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - SIMILARITY comments
Index of protein domains and families