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UniProtKB - Q4U2R1 (HERC2_MOUSE)

Protein

E3 ubiquitin-protein ligase HERC2

Gene

Herc2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

E3 ubiquitin-protein ligase that regulates ubiquitin-dependent retention of repair proteins on damaged chromosomes. Recruited to sites of DNA damage in response to ionizing radiation (IR) and facilitates the assembly of UBE2N and RNF8 promoting DNA damage-induced formation of 'Lys-63'-linked ubiquitin chains. Acts as a mediator of binding specificity between UBE2N and RNF8. Involved in the maintenance of RNF168 levels. E3 ubiquitin-protein ligase that promotes the ubiquitination and proteasomal degradation of XPA which influences the circadian oscillation of DNA excision repair activity. By controlling the steady-state expression of the IGF1R receptor, indirectly regulates the insulin-like growth factor receptor signaling pathway.By similarity

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei4764Glycyl thioester intermediatePROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri2703 – 2750ZZ-typePROSITE-ProRule annotationAdd BLAST48

GO - Molecular functioni

View the complete GO annotation on QuickGO ...

GO - Biological processi

View the complete GO annotation on QuickGO ...

Keywordsi

Molecular functionTransferase
Biological processDNA damage, DNA repair, Ubl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-3108214 SUMOylation of DNA damage response and repair proteins
R-MMU-5693565 Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks
R-MMU-5693571 Nonhomologous End-Joining (NHEJ)
R-MMU-69473 G2/M DNA damage checkpoint
R-MMU-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayi
UPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase HERC2 (EC:2.3.2.26By similarity)
Alternative name(s):
HECT domain and RCC1-like domain-containing protein 2
HECT-type E3 ubiquitin transferase HERC2
Gene namesi
Name:Herc2Imported
Synonyms:Jdf2, Kiaa0393Imported, Rjs
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:103234 Herc2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

Pathology & Biotechi

Involvement in diseasei

Defects in Herc2 are the cause of the runty, jerky, sterile phenotype (rjs), also known as the juvenile development and fertility phenotype (jfd2), which is characterized by reduced size, jerky gait, fertility problems including spermatocyte and oocyte abnormalities, defective maternal behavior and reduced lifespan with juvenile lethality.3 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002297401 – 4836E3 ubiquitin-protein ligase HERC2Add BLAST4836

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei273PhosphothreonineBy similarity1
Modified residuei648PhosphothreonineBy similarity1
Modified residuei1578PhosphoserineCombined sources1
Modified residuei1943PhosphoserineCombined sources1
Modified residuei1945PhosphothreonineCombined sources1
Modified residuei2455PhosphoserineBy similarity1
Modified residuei2929PhosphoserineCombined sources1
Modified residuei4812PhosphoserineBy similarity1
Modified residuei4813PhosphoserineBy similarity1
Modified residuei4816PhosphoserineBy similarity1
Modified residuei4829PhosphothreonineBy similarity1

Post-translational modificationi

Phosphorylation at Thr-4829 is required for interaction with RNF8.
Sumoylated with SUMO1 by PIAS4 in response to double-strand breaks (DSBs), promoting the interaction with RNF8.By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ4U2R1
PeptideAtlasiQ4U2R1
PRIDEiQ4U2R1

PTM databases

iPTMnetiQ4U2R1
PhosphoSitePlusiQ4U2R1

Expressioni

Tissue specificityi

Highest levels are found in brain and testis with lower levels in heart, lung, liver, skeletal muscle and kidney. Little expression detected in spleen.1 Publication

Gene expression databases

BgeeiENSMUSG00000030451 Expressed in 299 organ(s), highest expression level in skeletal muscle tissue
ExpressionAtlasiQ4U2R1 baseline and differential
GenevisibleiQ4U2R1 MM

Interactioni

Subunit structurei

Interacts (when phosphorylated at Thr-4829 and sumoylated) with RNF8 (via FHA domain); this interaction increases after ionising radiation (IR) treatment. Interacts with XPA. Interacts with NEURL4. Via its interaction with NEURL4, may indirectly interact with CCP110 and CEP97.By similarity

GO - Molecular functioni

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridi200274, 63 interactors
IntActiQ4U2R1, 59 interactors
STRINGi10090.ENSMUSP00000075579

Structurei

3D structure databases

ProteinModelPortaliQ4U2R1
SMRiQ4U2R1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati416 – 462RCC1 1-1Add BLAST47
Repeati463 – 513RCC1 1-2Add BLAST51
Repeati514 – 569RCC1 1-3Add BLAST56
Repeati570 – 621RCC1 1-4Add BLAST52
Repeati624 – 675RCC1 1-5Add BLAST52
Repeati676 – 727RCC1 1-6Add BLAST52
Repeati729 – 779RCC1 1-7Add BLAST51
Domaini1208 – 1284Cytochrome b5 heme-bindingPROSITE-ProRule annotationAdd BLAST77
Domaini1860 – 1933MIB/HERC2PROSITE-ProRule annotationAdd BLAST74
Domaini2760 – 2937DOCPROSITE-ProRule annotationAdd BLAST178
Repeati2959 – 3010RCC1 2-1Add BLAST52
Repeati3011 – 3065RCC1 2-2Add BLAST55
Repeati3066 – 3117RCC1 2-3Add BLAST52
Repeati3119 – 3169RCC1 2-4Add BLAST51
Repeati3172 – 3223RCC1 2-5Add BLAST52
Repeati3225 – 3275RCC1 2-6Add BLAST51
Repeati3276 – 3327RCC1 2-7Add BLAST52
Repeati3953 – 4004RCC1 3-1Add BLAST52
Repeati4006 – 4058RCC1 3-2Add BLAST53
Repeati4060 – 4110RCC1 3-3Add BLAST51
Repeati4112 – 4164RCC1 3-4Add BLAST53
Repeati4166 – 4216RCC1 3-5Add BLAST51
Repeati4218 – 4268RCC1 3-6Add BLAST51
Repeati4270 – 4320RCC1 3-7Add BLAST51
Domaini4459 – 4796HECTPROSITE-ProRule annotationAdd BLAST338

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili948 – 981Sequence analysisAdd BLAST34

Domaini

The ZZ-type zinc finger mediates binding to SUMO1, and at low level SUMO2.By similarity
The RCC1 repeats are grouped into three seven-bladed beta-propeller regions.By similarity

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri2703 – 2750ZZ-typePROSITE-ProRule annotationAdd BLAST48

Keywords - Domaini

Coiled coil, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1426 Eukaryota
COG5021 LUCA
COG5184 LUCA
GeneTreeiENSGT00910000144004
HOVERGENiHBG081598
InParanoidiQ4U2R1
KOiK10595
OMAiIMEMGFT
OrthoDBiEOG091G000E
TreeFamiTF320636

Family and domain databases

CDDicd08664 APC10-HERC2, 1 hit
cd00078 HECTc, 1 hit
Gene3Di2.130.10.30, 3 hits
2.30.30.30, 1 hit
2.30.30.920, 1 hit
2.60.120.260, 1 hit
3.10.120.10, 1 hit
InterProiView protein in InterPro
IPR004939 APC_su10/DOC_dom
IPR006624 Beta-propeller_rpt_TECPR
IPR021097 CPH_domain
IPR001199 Cyt_B5-like_heme/steroid-bd
IPR036400 Cyt_B5-like_heme/steroid_sf
IPR008979 Galactose-bd-like_sf
IPR000569 HECT_dom
IPR035983 Hect_E3_ubiquitin_ligase
IPR037976 HERC2_APC10
IPR010606 Mib_Herc2
IPR037252 Mib_Herc2_sf
IPR009091 RCC1/BLIP-II
IPR000408 Reg_chr_condens
IPR014722 Rib_L2_dom2
IPR000433 Znf_ZZ
PfamiView protein in Pfam
PF03256 ANAPC10, 1 hit
PF11515 Cul7, 1 hit
PF00173 Cyt-b5, 1 hit
PF00632 HECT, 1 hit
PF06701 MIB_HERC2, 1 hit
PF00415 RCC1, 16 hits
PF00569 ZZ, 1 hit
PRINTSiPR00633 RCCNDNSATION
SMARTiView protein in SMART
SM01337 APC10, 1 hit
SM01117 Cyt-b5, 1 hit
SM00119 HECTc, 1 hit
SM00706 TECPR, 4 hits
SM00291 ZnF_ZZ, 1 hit
SUPFAMiSSF159034 SSF159034, 1 hit
SSF49785 SSF49785, 1 hit
SSF50985 SSF50985, 3 hits
SSF55856 SSF55856, 1 hit
SSF56204 SSF56204, 1 hit
PROSITEiView protein in PROSITE
PS50255 CYTOCHROME_B5_2, 1 hit
PS51284 DOC, 1 hit
PS50237 HECT, 1 hit
PS51416 MIB_HERC2, 1 hit
PS00626 RCC1_2, 1 hit
PS50012 RCC1_3, 19 hits
PS01357 ZF_ZZ_1, 1 hit
PS50135 ZF_ZZ_2, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All

Isoform 12 Publications (identifier: Q4U2R1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MPSESFCLAA QSRLDSKWLK TDIQLAFTRD GLCGLWNEMV KDGEIVYTGT 
        60         70         80         90        100
ELAQNRELPL RKDDGVDAQS GTKKEDLNDK EKKEEEETPA PVYRAKSILE 
       110        120        130        140        150
SWVWGRQPDV NELKECLSVL VKEQQALAVQ SATTTLSALR LKQRLVILER 
       160        170        180        190        200
YFIALNRTVF QENVKVKWKS SSISVPPTEK KSARPTGRGV EGLARVGSRA 
       210        220        230        240        250
ALSFAFAFLR RAWRSGEDAD LCSELLQESL DALRALPEAS LFDESTVSSV 
       260        270        280        290        300
WLEVVERATR FLRSVVTGDV HGTPGTKGPG GVPLQDQHLA LAILLELAVQ 
       310        320        330        340        350
RGTLSQMLSA ILLLLQLWDS GAQETDNERS AQGTSAPLLP LLQRFQSIIC 
       360        370        380        390        400
SKDVPHTESD MHLLSGPLSP NESFLRYLTL PQDNELAIDL RQTAVVVMAH 
       410        420        430        440        450
LDRLATPCMP PLCSSPTSHK GSLQEVIGWG LIGWKYYANV IGPIQCEGLA 
       460        470        480        490        500
SLGVMQVACA EKRFLILSRN GRVYTQAYNS DMLAPQLVQG LASRNIVKIA 
       510        520        530        540        550
AHSDGHHYLA LAATGEVYSW GCGDGGRLGH GDTVPLEEPK VISAFSGKQA 
       560        570        580        590        600
GKHVVHIACG STYSAAITAE GELYTWGRGN YGRLGHGSSE DEAIPMLVAG 
       610        620        630        640        650
LKGLKVIDVA CGSGDAQTLA VTENGQVWSW GDGDYGKLGR GGSDGCKTPK 
       660        670        680        690        700
LIEKLQDLDV IKVRCGSQFS IALTKDGQVY SWGKGDNQRL GHGTEEHVRY 
       710        720        730        740        750
PKLLEGLQGK KVIDVAAGST HCLALTEDSE VHSWGSNDQC QHFDTLRVTK 
       760        770        780        790        800
PEPTALPGLD SKHIVGIACG PAQSFAWSSC SEWSIGLRVP FVVDICSMTF 
       810        820        830        840        850
EQLDLLLRQV SEGMDGTADW PPPQEKECMA VATLNLLRLQ LHAAISHQVD 
       860        870        880        890        900
PEFLGLGLGS VLLNSLKQTV VTLASSAGVL STVQSAAQAV LQSGWSVLLP 
       910        920        930        940        950
TAEERARALS ALLPCTVSGN EVNISPGRRF MIDLLVGSLM ADGGLESALN 
       960        970        980        990       1000
AAITAEIQDI EAKKEAQKEK EIDEQEASAS TFHRSRTPLD KDLINTGIYE 
      1010       1020       1030       1040       1050
SSGKQCLPLV QLIQQLLRNI ASQTVARLKD VARRISSCLD FEQQSCERSA 
      1060       1070       1080       1090       1100
SLDLLLRFQR LLISKLYPGE KIGPISDTSS PELMGVGSLL KKYTALVCTH 
      1110       1120       1130       1140       1150
IGDILPVAAS IASSSWQHFA EVACVMEGDF TGVLLPELVV SIVLLLSKNA 
      1160       1170       1180       1190       1200
SLMQEAGAIP LLGGLLEHLD RFNHLAPGKE RDDHEELAWP GIMESFFTGQ 
      1210       1220       1230       1240       1250
NCRNNEEVTL IRKADLENHN KDGGFWTVID GKVYGIKDFQ TQSLTGNSIL 
      1260       1270       1280       1290       1300
AQFAGEDPVV ALEAALQFED TQESMHAFCV GQYLEPDQEV VTIPDLGSLS 
      1310       1320       1330       1340       1350
SPLIDTERNL GLLLGLHASY LAMSTPLSPV EVECAKWLQS SIFSGGLQTS 
      1360       1370       1380       1390       1400
QIHYSYNEEK DEDHCSSPGG TPISKSRLCS HRWALGDHSQ AFLQAIADNN 
      1410       1420       1430       1440       1450
IQDYNVKDFL CQIERYCRQC HLTTPITFPP EHPVEEVGRL LLCCLLKHED 
      1460       1470       1480       1490       1500
LGHVALSLVH VGTLGIEQVK HRTLPKSVVD VCRVVYQAKC SLIKTHQEQG 
      1510       1520       1530       1540       1550
RSYKEVCAPV IERLRFLFNE LRPAVCSDLS IMSKFKLLGS LPRWRRIAQK 
      1560       1570       1580       1590       1600
IIRERRKKRV PKKPESIDSE EKIGNEESDL EEACVLPHSP INVDKRPISM 
      1610       1620       1630       1640       1650
KSPKDKWQPL LNTVTGVHKY KWLKQNVQGL YPQSALLNTI VEFALKEEPV 
      1660       1670       1680       1690       1700
DVEKMRKCLL KQLERAEVRL EGIDTILKLA AKSFLLPSVQ YAMFCGWQRL 
      1710       1720       1730       1740       1750
IPEGIDIGEP LTDCLRDVDL IPPFNRMLLE VTFGKLYAWA VQNIRSVLMD 
      1760       1770       1780       1790       1800
ASARFKELGI QPVPLQTITN ENPAGPSLGT IPQARFLLVM LSMLTLQHGA 
      1810       1820       1830       1840       1850
NNLDLLLNSG TLALTQTALR LIGPTCDSVE DDMNASARGA SATVLEETRK 
      1860       1870       1880       1890       1900
ETAPVQLPVS GPELAAMMKI GTRVMRGVDW KWGDQDGPPP GLGRVIGELG 
      1910       1920       1930       1940       1950
EDGWIRVQWD TGSTNSYRMG KEGKYDLKLV ELPVSSQPSA EDSDTEDDSE 
      1960       1970       1980       1990       2000
AEQGERNIHP TAMMLTSVIN LLQTLCLSVG VHADIMQSEA TKTLCGLLRM 
      2010       2020       2030       2040       2050
LVESGTTDKP APPDRLVARE QHRSWCTLGF VRSIALTPQA CGALSSPRWI 
      2060       2070       2080       2090       2100
TLLMKVVEGH APFTAASLQR QILAVHLLQA VLPSWDKTER ARDMKCLVEK 
      2110       2120       2130       2140       2150
LFGFLGSLLT TCSSDVPLLR ESTLRKRRAR PQASLTATHS STLAEEVVGL 
      2160       2170       2180       2190       2200
LRTLHSLTQW NGLINKYINS QLCSVTQSYA GKTSERAQLE DYFPDSENLE 
      2210       2220       2230       2240       2250
VGGLMAVLAV IGGIDGRLRL GGQVMHDEFG EGTVTRITPK GRITVQFCDM 
      2260       2270       2280       2290       2300
RMCRVCPLNQ LKPLPAVAFS VNNLPFTEPM LSVWAELVNL AGSKLEKHKT 
      2310       2320       2330       2340       2350
KKSAKPAFAG QVDLDLLRSQ QLKLYILKAG RALLSHQDKL RQILSQPAVQ 
      2360       2370       2380       2390       2400
GTGTLQTDDG AAASPDLGDM SPEGPQPPMI LLQQLLSSAT QPSPVKAIFD 
      2410       2420       2430       2440       2450
KQELEAAALA LCQCLAVEST HPSSPGCEDC SSSEATTPVS VQHIHLARAK 
      2460       2470       2480       2490       2500
KRRQSPAPAL PIVVQLMEMG FPRKNIEFAL KSLTGTSGNA SGLPGVEALV 
      2510       2520       2530       2540       2550
GWLLDHSDVQ VTEFSDAETL SDEYSDEEVV EDVDDTPYPV AAGAVVTESQ 
      2560       2570       2580       2590       2600
TYKKRADFLS NDDYAVYVRE NVQVGMMVRC CRTYEEVCEG DVGKVIKLDR 
      2610       2620       2630       2640       2650
DGLHDLNVQC DWQQKGGTYW VRYIHVELIG YPPPSSSSHI KIGDKVRVKA 
      2660       2670       2680       2690       2700
SVTTPKYKWG SVTHQSVGLV KAFSANGKDI IVDFPQQSHW TGLLSEMELV 
      2710       2720       2730       2740       2750
PSIHPGVTCD GCQTFPINGS RFKCRNCDDF DFCETCFKTK KHNTRHTFGR 
      2760       2770       2780       2790       2800
INEPGQSAVF CGRSGKQLKR CHSSQPGMLL DSWSRMVKSL NVSSSVNQAS 
      2810       2820       2830       2840       2850
RLIDGSEPCW QSSGSQGKHW IRLEIFPDVL VHRLKMIVDP ADSSYMPSLV 
      2860       2870       2880       2890       2900
VVSGGNSLNN LIELKTININ QTDTTVPLLS DCAEYHRYIE IAIKQCRSSG 
      2910       2920       2930       2940       2950
IDCKIHGLIL LGRIRAEEED LAAVPFLASD NEEEEDDKGS TGSLIRKKTP 
      2960       2970       2980       2990       3000
GLESTATIRT KVFVWGLNDK DQLGGLKGSK IKVPSFSETL SALNVVQVAG 
      3010       3020       3030       3040       3050
GSKSLFAVTV EGKVYSCGEA TNGRLGLGMS SGTVPIPRQI TALSSYVVKK 
      3060       3070       3080       3090       3100
VAVHSGGRHA TALTVDGKVF SWGEGDDGKL GHFSRMNCDK PRLIEALKTK 
      3110       3120       3130       3140       3150
RIRDIACGSS HSAALTSSGE LYTWGLGEYG RLGHGDNTTQ LKPKMVKVLL 
      3160       3170       3180       3190       3200
GHRVIQVACG SRDAQTLALT DEGLVFSWGD GDFGKLGRGG SEGCNIPQNI 
      3210       3220       3230       3240       3250
ERLNGQGVCQ IECGAQFSLA LTKSGVVWTW GKGDYFRLGH GSDVHVRKPQ 
      3260       3270       3280       3290       3300
VVEGLRGKKI VHVAVGALHC LAVTDSGQVY AWGDNDHGQQ GNGTTTVNRK 
      3310       3320       3330       3340       3350
PTLVQGLEGQ KITRVACGSS HSVAWTTVDV ATPSVHEPVL FQTARDPLGA 
      3360       3370       3380       3390       3400
SYLGVPSDAD SSSSSNKISG ANNCKPNRPS LAKILLSLEG NLAKQQALSH 
      3410       3420       3430       3440       3450
ILTALQIMYA RDAVVGALMP AGMLAPVECP SFSSSAPASD VSAMASPMHM 
      3460       3470       3480       3490       3500
EDSTLAADLE DRLSPNLWQE KREIVSSEDA VTPSAVTPSA PSASSRPFIP 
      3510       3520       3530       3540       3550
VTDDPGAASI IAETMTKTKE DVESQNKTSG PEPQSLDEFT SLLIPDDTRV 
      3560       3570       3580       3590       3600
VVELLKLSVC SRAGDKGREV LSAVLSGMGT AYPQVADMLL ELCVTELEDV 
      3610       3620       3630       3640       3650
ATDSQSGRLS SQPVVVESSH PYTDDTSTSG TVKIPGAEGL RVEFDRQCST 
      3660       3670       3680       3690       3700
ERRHDPLTVM DGVNRIVSVR SGREWSDWSS ELRIPGDELK WKFISDGSVN 
      3710       3720       3730       3740       3750
GWGWRFTVYP IMPAAGPKDL LSDRCVLSCP SMDLVTCLLD FRLNLTSNRS 
      3760       3770       3780       3790       3800
IVPRLAASLA ACAQLSALAA SHRMWALQRL RRLLTTEFGQ SININRLLGE 
      3810       3820       3830       3840       3850
NDGESRALSF TGSALAALVK GLPEALQRQF EYEDPIVRGG KQLLHSPFFK 
      3860       3870       3880       3890       3900
VLVALACDLE LDTLPCCAET HKWAWFRRYC MASRVAVALD KRTPLPRLFL 
      3910       3920       3930       3940       3950
DEVAKKIREL MADSESMDVL HESHSIFKRE QDEQLVQWMN RRPDDWTLSA 
      3960       3970       3980       3990       4000
GGSGTIYGWG HNHRGQLGGI EGAKVKVPTP CEALATLRPV QLIGGEQTLF 
      4010       4020       4030       4040       4050
AVTADGKLYA TGYGAGGRLG IGGTESVSTP TLLESIQHVF IKKVAVNSGG 
      4060       4070       4080       4090       4100
KHCLALSSEG EVYSWGEAED GKLGHGNRSP CDRPRVIESL RGIEVVDVAA 
      4110       4120       4130       4140       4150
GGAHSACVTA AGDLYTWGKG RYGRLGHSDS EDQLKPKLVE ALQGHRVIDI 
      4160       4170       4180       4190       4200
ACGSGDAQTL CLTDDDTVWS WGDGDYGKLG RGGSDGCKVP MKIDSLTGLG 
      4210       4220       4230       4240       4250
VVKVECGSQF SVALTKSGAV YTWGKGDYHR LGHGSDDHVR RPRQVQGLQG 
      4260       4270       4280       4290       4300
KKVIAIATGS LHCVCCTEDG EVYTWGDNDE GQLGDGTTNA IQRPRLVAAL 
      4310       4320       4330       4340       4350
QGKKVNRVAC GSAHTLAWST SKPASAGKLP AQVPMEYNHL QEIPIIALRN 
      4360       4370       4380       4390       4400
RLLLLHHISE LFCPCIPMFD LEGSLDETGL GPSVGFDTLR GILISQGKEA 
      4410       4420       4430       4440       4450
AFRKVVQATM VRDRQHGPVV ELNRIQVKRS RSKGGLAGPD GTKSVFGQMC 
      4460       4470       4480       4490       4500
AKMSSFSPDS LLLPHRVWKV KFVGESVDDC GGGYSESIAE ICEELQNGLT 
      4510       4520       4530       4540       4550
PLLIVTPNGR DESGANRDCY LLNPATRAPV HCSMFRFLGV LLGIAIRTGS 
      4560       4570       4580       4590       4600
PLSLNLAEPV WKQLAGMSLT IADLSEVDKD FIPGLMYIRD NEATSEEFEA 
      4610       4620       4630       4640       4650
MSLPFTVPSA SGQDIQLSSK HTHITLDNRA EYVRLAINYR LHEFDEQVAA 
      4660       4670       4680       4690       4700
VREGMARVVP VPLLSLFTGY ELETMVCGSP DIPLHLLKSV ATYKGIEPSA 
      4710       4720       4730       4740       4750
SLVQWFWEVM ESFSNTERSL FLRFVWGRTR LPRTIADFRG RDFVIQVLDK 
      4760       4770       4780       4790       4800
YNPPDHFLPE SYTCFFLLKL PRYSCKQVLE EKLKYAIHFC KSIDTDDYAR 
      4810       4820       4830 
IALTGEPAAD DSSEDSDNED ADSFASDSTQ DYLTGH
Length:4,836
Mass (Da):527,456
Last modified:July 27, 2011 - v3
Checksum:i481BC90E231C93D8
GO
Isoform 21 Publication (identifier: Q4U2R1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     3637-3672: Missing.

Note: No experimental confirmation available.Curated
Show »
Length:4,800
Mass (Da):523,388
Checksum:i3E3BA1D6DE63BAF7
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0U1RNG9A0A0U1RNG9_MOUSE
E3 ubiquitin-protein ligase HERC2
Herc2
167Annotation score:
A0A0U1RPZ3A0A0U1RPZ3_MOUSE
E3 ubiquitin-protein ligase HERC2
Herc2
965Annotation score:
A0A0U1RP01A0A0U1RP01_MOUSE
E3 ubiquitin-protein ligase HERC2
Herc2
40Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti692H → L in AAC31431 (PubMed:9689098).Curated1
Sequence conflicti724A → V in AAD08658 (PubMed:9949213).Curated1
Sequence conflicti747R → G in AAD08658 (PubMed:9949213).Curated1
Sequence conflicti756L → F in AAD08658 (PubMed:9949213).Curated1
Sequence conflicti929R → P in AAD08658 (PubMed:9949213).Curated1
Sequence conflicti1114S → N in AAD08658 (PubMed:9949213).Curated1
Sequence conflicti1235G → D in AAD08658 (PubMed:9949213).Curated1
Sequence conflicti2348A → P in AAC31431 (PubMed:9689098).Curated1
Sequence conflicti2470G → S in BAE36828 (PubMed:16141072).Curated1
Sequence conflicti2523E → D in AAC31431 (PubMed:9689098).Curated1
Sequence conflicti2567Y → F in AAC31431 (PubMed:9689098).Curated1
Sequence conflicti2572V → L in AAC31431 (PubMed:9689098).Curated1
Sequence conflicti3095E → Q in AAD08658 (PubMed:9949213).Curated1
Sequence conflicti3107C → Y in AAD08658 (PubMed:9949213).Curated1
Sequence conflicti3114A → P in AAD08658 (PubMed:9949213).Curated1
Sequence conflicti3161S → T in AAC31431 (PubMed:9689098).Curated1
Sequence conflicti3385 – 3386LL → VV in AAD08658 (PubMed:9949213).Curated2
Sequence conflicti3508A → V in AAD08658 (PubMed:9949213).Curated1
Sequence conflicti4069E → K in BAD90404 (Ref. 5) Curated1
Sequence conflicti4187C → S in AAC31431 (PubMed:9689098).Curated1
Sequence conflicti4604P → S in BAE36593 (PubMed:16141072).Curated1
Sequence conflicti4716T → A in AAC31431 (PubMed:9689098).Curated1
Sequence conflicti4723R → C in AAD08658 (PubMed:9949213).Curated1
Sequence conflicti4730R → S in AAC31431 (PubMed:9689098).Curated1
Sequence conflicti4752N → Y in AAC31431 (PubMed:9689098).Curated1
Sequence conflicti4790C → S in AAC31431 (PubMed:9689098).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0519753637 – 3672Missing in isoform 2. 1 PublicationAdd BLAST36

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF061529 mRNA Translation: AAC31431.1
AF071173 mRNA Translation: AAD08658.1
AC102121 Genomic DNA No translation available.
AC102150 Genomic DNA No translation available.
AK141515 mRNA Translation: BAE24711.1
AK148361 mRNA Translation: BAE28504.1
AK161826 mRNA Translation: BAE36593.1
AK162270 mRNA Translation: BAE36828.1
AK162708 mRNA Translation: BAE37031.1
AK220338 mRNA Translation: BAD90404.1
BC044667 mRNA Translation: AAH44667.1
BC054829 mRNA Translation: AAH54829.1
CCDSiCCDS21318.1 [Q4U2R1-1]
RefSeqiNP_034548.2, NM_010418.2 [Q4U2R1-1]
XP_006540700.1, XM_006540637.3 [Q4U2R1-1]
XP_006540701.1, XM_006540638.2
XP_006540702.1, XM_006540639.1 [Q4U2R1-2]
UniGeneiMm.20929

Genome annotation databases

EnsembliENSMUST00000076226; ENSMUSP00000075579; ENSMUSG00000030451 [Q4U2R1-1]
ENSMUST00000164095; ENSMUSP00000131573; ENSMUSG00000030451 [Q4U2R1-1]
ENSMUST00000205303; ENSMUSP00000145997; ENSMUSG00000030451 [Q4U2R1-2]
GeneIDi15204
KEGGimmu:15204
UCSCiuc009hdv.1 mouse [Q4U2R1-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF061529 mRNA Translation: AAC31431.1
AF071173 mRNA Translation: AAD08658.1
AC102121 Genomic DNA No translation available.
AC102150 Genomic DNA No translation available.
AK141515 mRNA Translation: BAE24711.1
AK148361 mRNA Translation: BAE28504.1
AK161826 mRNA Translation: BAE36593.1
AK162270 mRNA Translation: BAE36828.1
AK162708 mRNA Translation: BAE37031.1
AK220338 mRNA Translation: BAD90404.1
BC044667 mRNA Translation: AAH44667.1
BC054829 mRNA Translation: AAH54829.1
CCDSiCCDS21318.1 [Q4U2R1-1]
RefSeqiNP_034548.2, NM_010418.2 [Q4U2R1-1]
XP_006540700.1, XM_006540637.3 [Q4U2R1-1]
XP_006540701.1, XM_006540638.2
XP_006540702.1, XM_006540639.1 [Q4U2R1-2]
UniGeneiMm.20929

3D structure databases

ProteinModelPortaliQ4U2R1
SMRiQ4U2R1
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200274, 63 interactors
IntActiQ4U2R1, 59 interactors
STRINGi10090.ENSMUSP00000075579

PTM databases

iPTMnetiQ4U2R1
PhosphoSitePlusiQ4U2R1

Proteomic databases

PaxDbiQ4U2R1
PeptideAtlasiQ4U2R1
PRIDEiQ4U2R1

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000076226; ENSMUSP00000075579; ENSMUSG00000030451 [Q4U2R1-1]
ENSMUST00000164095; ENSMUSP00000131573; ENSMUSG00000030451 [Q4U2R1-1]
ENSMUST00000205303; ENSMUSP00000145997; ENSMUSG00000030451 [Q4U2R1-2]
GeneIDi15204
KEGGimmu:15204
UCSCiuc009hdv.1 mouse [Q4U2R1-1]

Organism-specific databases

CTDi8924
MGIiMGI:103234 Herc2
RougeiSearch...

Phylogenomic databases

eggNOGiKOG1426 Eukaryota
COG5021 LUCA
COG5184 LUCA
GeneTreeiENSGT00910000144004
HOVERGENiHBG081598
InParanoidiQ4U2R1
KOiK10595
OMAiIMEMGFT
OrthoDBiEOG091G000E
TreeFamiTF320636

Enzyme and pathway databases

UniPathwayi
UPA00143

ReactomeiR-MMU-3108214 SUMOylation of DNA damage response and repair proteins
R-MMU-5693565 Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks
R-MMU-5693571 Nonhomologous End-Joining (NHEJ)
R-MMU-69473 G2/M DNA damage checkpoint
R-MMU-983168 Antigen processing: Ubiquitination & Proteasome degradation

Miscellaneous databases

ChiTaRSiHerc2 mouse
PROiPR:Q4U2R1
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000030451 Expressed in 299 organ(s), highest expression level in skeletal muscle tissue
ExpressionAtlasiQ4U2R1 baseline and differential
GenevisibleiQ4U2R1 MM

Family and domain databases

CDDicd08664 APC10-HERC2, 1 hit
cd00078 HECTc, 1 hit
Gene3Di2.130.10.30, 3 hits
2.30.30.30, 1 hit
2.30.30.920, 1 hit
2.60.120.260, 1 hit
3.10.120.10, 1 hit
InterProiView protein in InterPro
IPR004939 APC_su10/DOC_dom
IPR006624 Beta-propeller_rpt_TECPR
IPR021097 CPH_domain
IPR001199 Cyt_B5-like_heme/steroid-bd
IPR036400 Cyt_B5-like_heme/steroid_sf
IPR008979 Galactose-bd-like_sf
IPR000569 HECT_dom
IPR035983 Hect_E3_ubiquitin_ligase
IPR037976 HERC2_APC10
IPR010606 Mib_Herc2
IPR037252 Mib_Herc2_sf
IPR009091 RCC1/BLIP-II
IPR000408 Reg_chr_condens
IPR014722 Rib_L2_dom2
IPR000433 Znf_ZZ
PfamiView protein in Pfam
PF03256 ANAPC10, 1 hit
PF11515 Cul7, 1 hit
PF00173 Cyt-b5, 1 hit
PF00632 HECT, 1 hit
PF06701 MIB_HERC2, 1 hit
PF00415 RCC1, 16 hits
PF00569 ZZ, 1 hit
PRINTSiPR00633 RCCNDNSATION
SMARTiView protein in SMART
SM01337 APC10, 1 hit
SM01117 Cyt-b5, 1 hit
SM00119 HECTc, 1 hit
SM00706 TECPR, 4 hits
SM00291 ZnF_ZZ, 1 hit
SUPFAMiSSF159034 SSF159034, 1 hit
SSF49785 SSF49785, 1 hit
SSF50985 SSF50985, 3 hits
SSF55856 SSF55856, 1 hit
SSF56204 SSF56204, 1 hit
PROSITEiView protein in PROSITE
PS50255 CYTOCHROME_B5_2, 1 hit
PS51284 DOC, 1 hit
PS50237 HECT, 1 hit
PS51416 MIB_HERC2, 1 hit
PS00626 RCC1_2, 1 hit
PS50012 RCC1_3, 19 hits
PS01357 ZF_ZZ_1, 1 hit
PS50135 ZF_ZZ_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiHERC2_MOUSE
AccessioniPrimary (citable) accession number: Q4U2R1
Secondary accession number(s): E9PZT6
, O88473, Q3TRJ8, Q3TS47, Q3TST2, Q3UFQ6, Q3URH7, Q5DU32, Q7TPR5, Q80VV7, Q9QYT1, Q9Z168, Q9Z171
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: July 27, 2011
Last modified: November 7, 2018
This is version 132 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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Main funding by: National Institutes of Health

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