UniProtKB - Q4TWH8 (NS1AB_HASV5)
Non-structural polyprotein 1AB
ORF1
Functioni
Contains the viral protease participating in the cleavage of the polyprotein into functional products. It contains also the activities necessary for replication of genomic RNA, as well as transcription of subgenomic mRNA.
Protein covalently attached to the 5' extremity of the genomic and subgenomic RNAs.
By similarityCatalytic activityi
- EC:2.7.7.48PROSITE-ProRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 461 | Charge relay system; for serine protease activityBy similarity | 1 | |
Active sitei | 489 | Charge relay system; for serine protease activityBy similarity | 1 | |
Active sitei | 551 | Charge relay system; for serine protease activityBy similarity | 1 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- cysteine-type peptidase activity Source: UniProtKB-KW
- RNA binding Source: InterPro
- RNA-directed 5'-3' RNA polymerase activity Source: UniProtKB-KW
- serine-type endopeptidase activity Source: InterPro
- serine-type exopeptidase activity Source: InterPro
GO - Biological processi
- transcription, DNA-templated Source: InterPro
- viral RNA genome replication Source: InterPro
Keywordsi
Molecular function | Hydrolase, Nucleotidyltransferase, Protease, RNA-directed RNA polymerase, Thiol protease, Transferase |
Biological process | Viral RNA replication |
Ligand | ATP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Non-structural polyprotein 1ABCleaved into the following 6 chains: |
Gene namesi | Name:ORF1 |
Organismi | Human astrovirus-5 (HAstV-5) |
Taxonomic identifieri | 35741 [NCBI] |
Taxonomic lineagei | Viruses › Riboviria › Orthornavirae › Pisuviricota › Stelpaviricetes › Stellavirales › Astroviridae › Mamastrovirus › |
Virus hosti | Homo sapiens (Human) [TaxID: 9606] |
Proteomesi |
|
Subcellular locationi
- Host membrane Curated; Multi-pass membrane protein Curated
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 154 – 174 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 239 – 259 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 286 – 306 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 313 – 333 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 344 – 364 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Host membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000327283 | 1 – 1416 | Non-structural polyprotein 1ABAdd BLAST | 1416 | |
ChainiPRO_0000327284 | 1 – 175 | Protein p19Sequence analysisAdd BLAST | 175 | |
ChainiPRO_0000327285 | 176 – 419 | Transmembrane protein 1ASequence analysisAdd BLAST | 244 | |
ChainiPRO_0000327286 | 420 – 664 | Serine protease p27Sequence analysisAdd BLAST | 245 | |
ChainiPRO_0000419588 | 665 – 755 | VPgSequence analysisAdd BLAST | 91 | |
ChainiPRO_0000327287 | 756 – 913 | Protein p20Sequence analysisAdd BLAST | 158 | |
ChainiPRO_0000327288 | 914 – 1416 | RNA-directed RNA polymerase p57Sequence analysisAdd BLAST | 503 |
Post-translational modificationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 175 – 176 | CleavageSequence analysis | 2 | |
Sitei | 419 – 420 | CleavageSequence analysis | 2 | |
Sitei | 664 – 665 | CleavageSequence analysis | 2 | |
Sitei | 755 – 756 | CleavageSequence analysis | 2 | |
Sitei | 913 – 914 | CleavageSequence analysis | 2 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1161 – 1287 | RdRp catalyticPROSITE-ProRule annotationAdd BLAST | 127 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 752 – 815 | DisorderedSequence analysisAdd BLAST | 64 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 104 – 146 | Sequence analysisAdd BLAST | 43 | |
Coiled coili | 587 – 614 | Sequence analysisAdd BLAST | 28 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 779 – 799 | Basic and acidic residuesSequence analysisAdd BLAST | 21 |
Sequence similaritiesi
Keywords - Domaini
Coiled coil, Transmembrane, Transmembrane helixFamily and domain databases
Gene3Di | 2.40.10.10, 2 hits 3.30.70.270, 1 hit |
InterProi | View protein in InterPro IPR045835, Astro_1A IPR045833, Astro_p19 IPR045836, Astro_VPg IPR043502, DNA/RNA_pol_sf IPR022068, Mamastrovirus_p20 IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR043128, Rev_trsase/Diguanyl_cyclase IPR001205, RNA-dir_pol_C IPR007094, RNA-dir_pol_PSvirus |
Pfami | View protein in Pfam PF19415, Astro_1A, 1 hit PF19414, Astro_p19, 1 hit PF19416, Astro_VPg, 1 hit PF12285, DUF3621, 1 hit PF00680, RdRP_1, 1 hit |
SUPFAMi | SSF50494, SSF50494, 1 hit SSF56672, SSF56672, 1 hit |
PROSITEi | View protein in PROSITE PS50507, RDRP_SSRNA_POS, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsiribosomal frameshifting. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAHGEPYYSS KPDKDFNFGS TMARRQMTPT MVTKLPKFVR NSPQVYDWIV
60 70 80 90 100
RGLIFPTTGK TYFQRVVVIT GGFEDGTYGS FAFDGREWVE IYPIEHLNLM
110 120 130 140 150
SSLKLIHKAN ALQERLRLSQ EEKATLTLDV QFLQHENVRL KELISKPEPR
160 170 180 190 200
KIQMKWIIVG AVLTFLSLIP GGYAQSQTNN TIFTDVIAAC KYSTETLTEN
210 220 230 240 250
LDLRIKLALA NITISDKLDA VRQILNFAFV PRSHWLRTVF YYIHYYEMWN
260 270 280 290 300
IFMFVLAIGT VMRSTRPGTD LITLATSHLS GFRMAVLPTI PFHTTMTLWV
310 320 330 340 350
MNTLMVCYYF DNLLAITMAI LAPILGIIFL CFMEDSNYVS QIRGLIATAV
360 370 380 390 400
LIAGGHACLT LTGTTTSLFV VILTCRFVRM ATIFIGTRFE IRDANGKVVA
410 420 430 440 450
TVPTRIKNAA FDFFQRLKQS GVRVGVNEFV VIKPGALCVI DTPEGKGTGF
460 470 480 490 500
FSGNDIVTAA HVVGNNTFVN VCYEGLMYEA KVRYMPEKDI AFITCPGDLH
510 520 530 540 550
PTARLKLSKN PDYSCVTVMA YVNEDLVVST AAAMVHGNTL SYAVRTQDGM
560 570 580 590 600
SGAPVCDKYG RVLAVHQTNT GYTGGAVIID PADFHPVKAP SQVELLKEEI
610 620 630 640 650
ERLKAQLNSA AENPSTVITQ QPTATLEQKS VNDSDVVDLV RTAMEREMKI
660 670 680 690 700
LRDEINGILA PFLQKKKGKT KHGRGRVRRN LRKGVKLLTE EEYRELLEKG
710 720 730 740 750
LDRETFLDLI DRIIGERSGY PDYDDEDYYD EDDDGWGMVG DDVEFDYTEV
760 770 780 790 800
INFDQAKPTP APRTTKPKPC PEPEAETQPL DLSQKKDKQL EHEQQVVKPT
810 820 830 840 850
KPQKNDPQPY SQTYGKAPIW ESYDFDWDED DAKFILPAPP RLTKADEIVL
860 870 880 890 900
GSKIVKLRTI IETAIKTQNY SALPEAVFEL DKAAYEAGLE GFLQRVKSKN
910 920 930 940 950
KAPKKLQRAP EDQGAQNYHS LDAWKSLLEP PRERRCVPAN FPLLGHLPID
960 970 980 990 1000
RPIFDDKKPR DDLLGLLPEP TWHAFEEYGP TTWGPQAFIK SFDKFFYAEP
1010 1020 1030 1040 1050
IDFFSEYPQL CAFADWATYR EFRYLEDTRV IHITATEKNT DSTPAYPKMN
1060 1070 1080 1090 1100
YFDTEEKYLE SYGWAPYIRE FTRVFKGDKP EVLWYLFLKK EIIKEEKIRN
1110 1120 1130 1140 1150
SDIRQIVCAD PIYTRIGACL EAHQNALMKQ HTETSVGQCG WSPMEGGFKK
1160 1170 1180 1190 1200
TMQRLVNKGN KYFIEFDWTR YDGTIPPSLF RHIKEIRWNF INKDQREKYR
1210 1220 1230 1240 1250
HVHEWYVDNL LNRHVLLPSG EVTLQTRGNP SGQFSTTMDN NMINFWLQAF
1260 1270 1280 1290 1300
EFAYFNGPNK DLWKTYDTVV YGDDRLSTTP SVPENYEERV IDMYRDIFGM
1310 1320 1330 1340 1350
WVKPGKVICR ESIVGLSFCG FTVNADLEPV PTSPEKLMAS LLKPYKILPD
1360 1370 1380 1390 1400
LESLHGKLLC YQLLAAFMAE DHPFKVYVEH CLSRTAKQLR DSGLPARLTE
1410
EQLHRIWRGG PKKCDG
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | DQ028633 Genomic RNA Translation: AAY46273.1 Sequence problems. |
Keywords - Coding sequence diversityi
Ribosomal frameshiftingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | DQ028633 Genomic RNA Translation: AAY46273.1 Sequence problems. |
3D structure databases
SMRi | Q4TWH8 |
ModBasei | Search... |
Family and domain databases
Gene3Di | 2.40.10.10, 2 hits 3.30.70.270, 1 hit |
InterProi | View protein in InterPro IPR045835, Astro_1A IPR045833, Astro_p19 IPR045836, Astro_VPg IPR043502, DNA/RNA_pol_sf IPR022068, Mamastrovirus_p20 IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR043128, Rev_trsase/Diguanyl_cyclase IPR001205, RNA-dir_pol_C IPR007094, RNA-dir_pol_PSvirus |
Pfami | View protein in Pfam PF19415, Astro_1A, 1 hit PF19414, Astro_p19, 1 hit PF19416, Astro_VPg, 1 hit PF12285, DUF3621, 1 hit PF00680, RdRP_1, 1 hit |
SUPFAMi | SSF50494, SSF50494, 1 hit SSF56672, SSF56672, 1 hit |
PROSITEi | View protein in PROSITE PS50507, RDRP_SSRNA_POS, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | NS1AB_HASV5 | |
Accessioni | Q4TWH8Primary (citable) accession number: Q4TWH8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 8, 2008 |
Last sequence update: | April 8, 2008 | |
Last modified: | February 23, 2022 | |
This is version 70 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Viral Protein Annotation Program |