UniProtKB - Q4R8B6 (F264_MACFA)
Protein
6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4
Gene
PFKFB4
Organism
Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Status
Functioni
Synthesis and degradation of fructose 2,6-bisphosphate.By similarity
Catalytic activityi
Activity regulationi
The most important regulatory mechanism of these opposing activities is by phosphorylation and dephosphorylation of the enzyme.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 79 | Fructose 6-phosphateBy similarity | 1 | |
Binding sitei | 103 | Fructose 6-phosphateBy similarity | 1 | |
Active sitei | 129 | Sequence analysis | 1 | |
Binding sitei | 131 | Fructose 6-phosphateBy similarity | 1 | |
Binding sitei | 137 | Fructose 6-phosphateBy similarity | 1 | |
Active sitei | 159 | Sequence analysis | 1 | |
Binding sitei | 173 | Fructose 6-phosphateBy similarity | 1 | |
Binding sitei | 194 | Fructose 6-phosphateBy similarity | 1 | |
Binding sitei | 198 | Fructose 6-phosphateBy similarity | 1 | |
Binding sitei | 256 | Fructose 2,6-bisphosphateBy similarity | 1 | |
Active sitei | 257 | Tele-phosphohistidine intermediateBy similarity | 1 | |
Binding sitei | 263 | Fructose 2,6-bisphosphateBy similarity | 1 | |
Binding sitei | 269 | Fructose 2,6-bisphosphate; via amide nitrogenBy similarity | 1 | |
Binding sitei | 306 | Fructose 2,6-bisphosphateBy similarity | 1 | |
Active sitei | 326 | Proton donor/acceptorBy similarity | 1 | |
Binding sitei | 337 | Fructose 2,6-bisphosphateBy similarity | 1 | |
Binding sitei | 351 | Fructose 2,6-bisphosphateBy similarity | 1 | |
Binding sitei | 355 | Fructose 2,6-bisphosphateBy similarity | 1 | |
Binding sitei | 366 | Fructose 2,6-bisphosphateBy similarity | 1 | |
Sitei | 391 | Transition state stabilizerBy similarity | 1 | |
Binding sitei | 392 | Fructose 2,6-bisphosphateBy similarity | 1 | |
Binding sitei | 396 | Fructose 2,6-bisphosphateBy similarity | 1 | |
Binding sitei | 428 | ATPBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 46 – 54 | ATPBy similarity | 9 | |
Nucleotide bindingi | 168 – 173 | ATPBy similarity | 6 | |
Nucleotide bindingi | 348 – 351 | ATPBy similarity | 4 | |
Nucleotide bindingi | 392 – 396 | ATPBy similarity | 5 |
GO - Molecular functioni
- 6-phosphofructo-2-kinase activity Source: UniProtKB-EC
- ATP binding Source: UniProtKB-KW
- fructose-2,6-bisphosphate 2-phosphatase activity Source: UniProtKB-EC
GO - Biological processi
- fructose 2,6-bisphosphate metabolic process Source: InterPro
- fructose metabolic process Source: InterPro
Keywordsi
Molecular function | Hydrolase, Kinase, Multifunctional enzyme, Transferase |
Ligand | ATP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4Short name: 6PF-2-K/Fru-2,6-P2ase 4 Short name: PFK/FBPase 4 Alternative name(s): 6PF-2-K/Fru-2,6-P2ase testis-type isozyme Including the following 2 domains: Fructose-2,6-bisphosphatase (EC:3.1.3.46) |
Gene namesi | Name:PFKFB4 ORF Names:QtsA-12874 |
Organismi | Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) |
Taxonomic identifieri | 9541 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Cercopithecidae › Cercopithecinae › Macaca |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000268186 | 1 – 469 | 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4Add BLAST | 469 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 444 | Phosphothreonine; by PKCSequence analysis | 1 |
Keywords - PTMi
PhosphoproteinInteractioni
Subunit structurei
Homodimer.
By similarityProtein-protein interaction databases
STRINGi | 9541.XP_005547086.1 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 249 | 6-phosphofructo-2-kinaseAdd BLAST | 249 | |
Regioni | 250 – 469 | Fructose-2,6-bisphosphataseAdd BLAST | 220 |
Sequence similaritiesi
In the C-terminal section; belongs to the phosphoglycerate mutase family.Curated
Phylogenomic databases
eggNOGi | KOG0234, Eukaryota |
GeneTreei | ENSGT00950000182835 |
OMAi | HRNRPQN |
OrthoDBi | 392001at2759 |
Family and domain databases
CDDi | cd07067, HP_PGM_like, 1 hit |
Gene3Di | 3.40.50.1240, 1 hit |
InterProi | View protein in InterPro IPR003094, 6Pfruct_kin IPR013079, 6Phosfructo_kin IPR013078, His_Pase_superF_clade-1 IPR029033, His_PPase_superfam IPR027417, P-loop_NTPase IPR001345, PG/BPGM_mutase_AS |
PANTHERi | PTHR10606, PTHR10606, 1 hit |
Pfami | View protein in Pfam PF01591, 6PF2K, 1 hit PF00300, His_Phos_1, 1 hit |
PRINTSi | PR00991, 6PFRUCTKNASE |
SMARTi | View protein in SMART SM00855, PGAM, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit SSF53254, SSF53254, 1 hit |
PROSITEi | View protein in PROSITE PS00175, PG_MUTASE, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
Q4R8B6-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MASPRELTQN PLKKIWMPYS NGRPALHACQ RGVCMTNCPT LIVMVGLPAR
60 70 80 90 100
GKTYISKKLT RYLNWIGVPT REFNVGQYRR NMVKTYKSFE FFLPDNEEGL
110 120 130 140 150
KIRKQCALAA LRDVRRFLSE EGGHVAVFDA TNTTRERRAT IFNFGEQNGY
160 170 180 190 200
KTFFVESICV DPEVIAANIV QVKLGSPDYV NRDSDEATED FMRRIECYEN
210 220 230 240 250
SYESLDEDLD RDLSYIKIMD VGQSYVVNRV ADHIQSRIVY YLMNIHVTPR
260 270 280 290 300
SIYLCRHGES ELNLKGRIGG DPGLSPRGRE FAKSLAQFIS DQNIKDLKVW
310 320 330 340 350
TSQMKRTIQT AEALGVPYEQ WKVLNEIDAG VCEEMTYEEI QDNYPLEFAL
360 370 380 390 400
RDQDKYRYRY PKGESYEDLV QRLEPVIMEL ERQENVLVIC HQAVMRCLLA
410 420 430 440 450
YFLDKAAEQL PYLKCPLHTV LKLTPVAYGC KVESIFLNVA AVNTHRDRPQ
460
NVDISRPPEE ALVTVPAHQ
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A2K5TUD5 | A0A2K5TUD5_MACFA | 6-phosphofructo-2-kinase | 372 | Annotation score: | |||
A0A2K5TUE4 | A0A2K5TUE4_MACFA | 6-phosphofructo-2-kinase | 434 | Annotation score: | |||
A0A2K5TUF5 | A0A2K5TUF5_MACFA | Uncharacterized protein | 93 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB168541 mRNA Translation: BAE00656.1 |
RefSeqi | NP_001270781.1, NM_001283852.1 |
Genome annotation databases
Ensembli | ENSMFAT00000022319; ENSMFAP00000003658; ENSMFAG00000001535 |
GeneIDi | 101925992 |
KEGGi | mcf:101925992 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB168541 mRNA Translation: BAE00656.1 |
RefSeqi | NP_001270781.1, NM_001283852.1 |
3D structure databases
SMRi | Q4R8B6 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9541.XP_005547086.1 |
Genome annotation databases
Ensembli | ENSMFAT00000022319; ENSMFAP00000003658; ENSMFAG00000001535 |
GeneIDi | 101925992 |
KEGGi | mcf:101925992 |
Organism-specific databases
CTDi | 5210 |
Phylogenomic databases
eggNOGi | KOG0234, Eukaryota |
GeneTreei | ENSGT00950000182835 |
OMAi | HRNRPQN |
OrthoDBi | 392001at2759 |
Family and domain databases
CDDi | cd07067, HP_PGM_like, 1 hit |
Gene3Di | 3.40.50.1240, 1 hit |
InterProi | View protein in InterPro IPR003094, 6Pfruct_kin IPR013079, 6Phosfructo_kin IPR013078, His_Pase_superF_clade-1 IPR029033, His_PPase_superfam IPR027417, P-loop_NTPase IPR001345, PG/BPGM_mutase_AS |
PANTHERi | PTHR10606, PTHR10606, 1 hit |
Pfami | View protein in Pfam PF01591, 6PF2K, 1 hit PF00300, His_Phos_1, 1 hit |
PRINTSi | PR00991, 6PFRUCTKNASE |
SMARTi | View protein in SMART SM00855, PGAM, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit SSF53254, SSF53254, 1 hit |
PROSITEi | View protein in PROSITE PS00175, PG_MUTASE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | F264_MACFA | |
Accessioni | Q4R8B6Primary (citable) accession number: Q4R8B6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 12, 2006 |
Last sequence update: | January 23, 2007 | |
Last modified: | December 2, 2020 | |
This is version 68 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families