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UniProtKB - Q4QQW3 (HOT_RAT)
Protein
Hydroxyacid-oxoacid transhydrogenase, mitochondrial
Gene
Adhfe1
Organism
Rattus norvegicus (Rat)
Status
Functioni
Catalyzes the cofactor-independent reversible oxidation of gamma-hydroxybutyrate (GHB) to succinic semialdehyde (SSA) coupled to reduction of 2-ketoglutarate (2-KG) to D-2-hydroxyglutarate (D-2-HG). L-3-hydroxybutyrate (L-3-OHB) is also a substrate for HOT when using 2-KG as hydrogen acceptor, resulting in the formation of D-2-HG (By similarity).
By similarityCatalytic activityi
Kineticsi
- KM=0.3 mM for GHB1 Publication
- KM=0.4 mM for D-2-HG1 Publication
- KM=0.018 mM for 2-KG1 Publication
- KM=0.005 mM for SSA1 Publication
- KM=3 mM for L-3-OHB1 Publication
GO - Molecular functioni
- alcohol dehydrogenase (NAD+) activity Source: GO_Central
- hydroxyacid-oxoacid transhydrogenase activity Source: UniProtKB
- metal ion binding Source: InterPro
GO - Biological processi
- glutamate catabolic process via 2-oxoglutarate Source: UniProtKB
- lipid metabolic process Source: UniProtKB-KW
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Lipid metabolism |
Enzyme and pathway databases
Reactomei | R-RNO-880009, Interconversion of 2-oxoglutarate and 2-hydroxyglutarate |
SABIO-RKi | Q4QQW3 |
Names & Taxonomyi
Protein namesi | Recommended name: Hydroxyacid-oxoacid transhydrogenase, mitochondrial (EC:1.1.99.24)Short name: HOT Alternative name(s): Alcohol dehydrogenase iron-containing protein 1 Short name: ADHFe1 |
Gene namesi | Name:Adhfe1 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 1308863, Adhfe1 |
Subcellular locationi
Mitochondrion
- Mitochondrion 1 Publication
Mitochondrion
- mitochondrion Source: UniProtKB
Keywords - Cellular componenti
MitochondrionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – ? | MitochondrionSequence analysis | ||
ChainiPRO_0000322999 | ? – 467 | Hydroxyacid-oxoacid transhydrogenase, mitochondrial |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 445 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 452 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
PaxDbi | Q4QQW3 |
PRIDEi | Q4QQW3 |
PTM databases
iPTMneti | Q4QQW3 |
PhosphoSitePlusi | Q4QQW3 |
Expressioni
Tissue specificityi
Expressed in kidney and liver.1 Publication
Gene expression databases
Bgeei | ENSRNOG00000007069, Expressed in liver and 21 other tissues |
Genevisiblei | Q4QQW3, RN |
Interactioni
Protein-protein interaction databases
IntActi | Q4QQW3, 1 interactor |
STRINGi | 10116.ENSRNOP00000009462 |
Family & Domainsi
Sequence similaritiesi
Belongs to the iron-containing alcohol dehydrogenase family. Hydroxyacid-oxoacid transhydrogenase subfamily.Curated
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | KOG3857, Eukaryota |
GeneTreei | ENSGT00390000003849 |
HOGENOMi | CLU_007207_0_7_1 |
InParanoidi | Q4QQW3 |
OMAi | IRFGPGC |
OrthoDBi | 776004at2759 |
PhylomeDBi | Q4QQW3 |
TreeFami | TF105710 |
Family and domain databases
CDDi | cd08190, HOT, 1 hit |
InterProi | View protein in InterPro IPR001670, ADH_Fe/GldA IPR039697, Alcohol_dehydrogenase_Fe IPR042157, HOT |
PANTHERi | PTHR11496, PTHR11496, 1 hit |
Pfami | View protein in Pfam PF00465, Fe-ADH, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q4QQW3-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAAAARARVT HLLRHLQSTA CQCPTHSHTY SQVPGLSPSG KTTDYAFEMA
60 70 80 90 100
VSNIRYGAGV TKEVGMDLQN MGAKNVCLMT DKNLSQLPPV QIVMDSLSKN
110 120 130 140 150
GISFQVYDNV RVEPTDGSFM DAIEFAKKGA FDAYVAVGGG STMDTCKAAN
160 170 180 190 200
LYACSPHSEF LDYVNAPIGK GKPVTVPLKP LIAVPTTSGT GSETTGVAIF
210 220 230 240 250
DYEHLKVKTG IASRAIKPTL GLVDPLHTLH MPCQVVANSG FDVLCHALES
260 270 280 290 300
YTAIPYSMRS PCPSNPIQRP AYQGSNPISD IWAVHALRIV AKYLKRAVRN
310 320 330 340 350
PDDLEARSSM HLASAFAGIG FGNAGVHLCH GMSYPISGLV KTYKAKEYNV
360 370 380 390 400
DHPLVPHGLS VVLTSPAVFT FTAQMFPERH LETAEILGAN IRTAKIQDAG
410 420 430 440 450
PVLADALRKF LFDLNVDDGL AALGYSKDDI PSLVKGTLPQ ERVTKLAPRA
460
QSEEDLSALF EASMKLY
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC097945 mRNA Translation: AAH97945.1 |
RefSeqi | NP_001020594.1, NM_001025423.1 |
Genome annotation databases
Ensembli | ENSRNOT00000009462; ENSRNOP00000009462; ENSRNOG00000007069 |
GeneIDi | 362474 |
KEGGi | rno:362474 |
UCSCi | RGD:1308863, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC097945 mRNA Translation: AAH97945.1 |
RefSeqi | NP_001020594.1, NM_001025423.1 |
3D structure databases
SMRi | Q4QQW3 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | Q4QQW3, 1 interactor |
STRINGi | 10116.ENSRNOP00000009462 |
PTM databases
iPTMneti | Q4QQW3 |
PhosphoSitePlusi | Q4QQW3 |
Proteomic databases
PaxDbi | Q4QQW3 |
PRIDEi | Q4QQW3 |
Genome annotation databases
Ensembli | ENSRNOT00000009462; ENSRNOP00000009462; ENSRNOG00000007069 |
GeneIDi | 362474 |
KEGGi | rno:362474 |
UCSCi | RGD:1308863, rat |
Organism-specific databases
CTDi | 137872 |
RGDi | 1308863, Adhfe1 |
Phylogenomic databases
eggNOGi | KOG3857, Eukaryota |
GeneTreei | ENSGT00390000003849 |
HOGENOMi | CLU_007207_0_7_1 |
InParanoidi | Q4QQW3 |
OMAi | IRFGPGC |
OrthoDBi | 776004at2759 |
PhylomeDBi | Q4QQW3 |
TreeFami | TF105710 |
Enzyme and pathway databases
Reactomei | R-RNO-880009, Interconversion of 2-oxoglutarate and 2-hydroxyglutarate |
SABIO-RKi | Q4QQW3 |
Miscellaneous databases
PROi | PR:Q4QQW3 |
Gene expression databases
Bgeei | ENSRNOG00000007069, Expressed in liver and 21 other tissues |
Genevisiblei | Q4QQW3, RN |
Family and domain databases
CDDi | cd08190, HOT, 1 hit |
InterProi | View protein in InterPro IPR001670, ADH_Fe/GldA IPR039697, Alcohol_dehydrogenase_Fe IPR042157, HOT |
PANTHERi | PTHR11496, PTHR11496, 1 hit |
Pfami | View protein in Pfam PF00465, Fe-ADH, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | HOT_RAT | |
Accessioni | Q4QQW3Primary (citable) accession number: Q4QQW3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 18, 2008 |
Last sequence update: | July 19, 2005 | |
Last modified: | February 23, 2022 | |
This is version 91 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families