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UniProtKB - Q4P9K9 (CHS8_USTMA)
Protein
Chitin synthase 8
Gene
CHS8
Organism
Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus)
Status
Functioni
Polymerizes chitin, a structural polymer of the cell wall and septum, by transferring the sugar moiety of UDP-GlcNAc to the non-reducing end of the growing chitin polymer (Probable). Involved in mating tube and dikaryotic hyphae formation and required for the formation of invading hyphae during plant infection (PubMed:16314447).
Curated1 PublicationCatalytic activityi
- [(1→4)-N-acetyl-β-D-glucosaminyl](n) + UDP-N-acetyl-α-D-glucosamine = [(1→4)-N-acetyl-β-D-glucosaminyl](n+1) + H+ + UDPEC:2.4.1.16
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 108 – 115 | ATPPROSITE-ProRule annotation | 8 |
GO - Molecular functioni
- actin binding Source: UniProtKB-KW
- ATP binding Source: UniProtKB-KW
- chitin synthase activity Source: GO_Central
- cytoskeletal motor activity Source: InterPro
GO - Biological processi
- cell wall organization Source: UniProtKB-KW
Keywordsi
Molecular function | Actin-binding, Glycosyltransferase, Motor protein, Myosin, Transferase |
Biological process | Cell wall biogenesis/degradation |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 2.4.1.16, 6587 |
Names & Taxonomyi
Protein namesi | Recommended name: Chitin synthase 8 (EC:2.4.1.16)Alternative name(s): Chitin-UDP acetyl-glucosaminyl transferase 8 Myosin chitin synthase 1 |
Gene namesi | Name:CHS8 Synonyms:MCS1 ORF Names:UMAG_03204 |
Organismi | Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus) |
Taxonomic identifieri | 237631 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Basidiomycota › Ustilaginomycotina › Ustilaginomycetes › Ustilaginales › Ustilaginaceae › Ustilago › |
Proteomesi |
|
Organism-specific databases
VEuPathDBi | FungiDB:UMAG_03204 |
Subcellular locationi
Plasma membrane
- Cell membrane 1 Publication; Multi-pass membrane protein Sequence analysis
Other locations
- Cytoplasmic vesicle membrane 1 Publication; Multi-pass membrane protein Sequence analysis
Note: A constitutive cytoplasmic pool is present that localizes to intracellular microvesicles termed chitosomes. Chitosomes constitute a separate secretory route distinct from the typical secretory pathway and serve as a vehicle for delivering the enzyme to the sites on the cell surface where polysaccharide sythesis takes place. Localizes to septa of yeast-like cells and to the basal septum separating the living tip cell from the vacuolated part in hyphae. Also localizes to the growing bud tip in yeast-like cells and in a tip-ward gradient at the hyphal apex.2 Publications
Cytoskeleton
- myosin complex Source: UniProtKB-KW
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Other locations
- cell periphery Source: GO_Central
- cell septum Source: GO_Central
- cytoplasmic vesicle membrane Source: UniProtKB-SubCell
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 929 – 949 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 965 – 985 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 1232 – 1252 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 1604 – 1624 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 1626 – 1646 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 1653 – 1673 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 1680 – 1700 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Cell membrane, Cytoplasmic vesicle, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000270624 | 1 – 2005 | Chitin synthase 8Add BLAST | 2005 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 164 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 364 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 390 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 546 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 1076 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 1650 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 1770 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 1794 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 1882 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
GlycoproteinProteomic databases
PRIDEi | Q4P9K9 |
Interactioni
GO - Molecular functioni
- actin binding Source: UniProtKB-KW
Protein-protein interaction databases
IntActi | Q4P9K9, 1 interactor |
MINTi | Q4P9K9 |
STRINGi | 5270.UM03204P0 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 5 – 773 | Myosin motorPROSITE-ProRule annotationAdd BLAST | 769 | |
Domaini | 1949 – 2002 | DEK C-terminalSequence analysisAdd BLAST | 54 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 585 – 631 | DisorderedSequence analysisAdd BLAST | 47 | |
Regioni | 647 – 669 | Actin-bindingPROSITE-ProRule annotationAdd BLAST | 23 | |
Regioni | 1796 – 1821 | DisorderedSequence analysisAdd BLAST | 26 | |
Regioni | 1912 – 1950 | DisorderedSequence analysisAdd BLAST | 39 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 585 – 606 | Polar residuesSequence analysisAdd BLAST | 22 | |
Compositional biasi | 1912 – 1933 | Polar residuesSequence analysisAdd BLAST | 22 |
Sequence similaritiesi
In the N-terminal section; belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.Curated
Keywords - Domaini
Repeat, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG2571, Eukaryota KOG4229, Eukaryota |
HOGENOMi | CLU_000192_0_2_1 |
InParanoidi | Q4P9K9 |
OMAi | MRYDDKR |
OrthoDBi | 20724at2759 |
Family and domain databases
CDDi | cd14879, MYSc_Myo17, 1 hit |
Gene3Di | 3.40.850.10, 2 hits |
InterProi | View protein in InterPro IPR004835, Chitin_synth IPR001199, Cyt_B5-like_heme/steroid-bd IPR036400, Cyt_B5-like_heme/steroid_sf IPR014876, DEK_C IPR036961, Kinesin_motor_dom_sf IPR001609, Myosin_head_motor_dom IPR036037, MYSc_Myo17 IPR029044, Nucleotide-diphossugar_trans IPR027417, P-loop_NTPase |
PANTHERi | PTHR22914, PTHR22914, 1 hit |
Pfami | View protein in Pfam PF00173, Cyt-b5, 1 hit PF08766, DEK_C, 1 hit PF00063, Myosin_head, 1 hit |
PRINTSi | PR00193, MYOSINHEAVY |
SMARTi | View protein in SMART SM01117, Cyt-b5, 2 hits SM00242, MYSc, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit SSF53448, SSF53448, 1 hit SSF55856, SSF55856, 1 hit |
PROSITEi | View protein in PROSITE PS51456, MYOSIN_MOTOR, 1 hit |
i Sequence
Sequence statusi: Complete.
Q4P9K9-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSALDEVAKL SQLTNITPDT IFSVLRDRFY AGLPYTALSD SILVSVNPYA
60 70 80 90 100
SSGNRNSDDT LREYTSDYRQ TNKQLRAATL PPHIFAHACN AYFYMRRTGQ
110 120 130 140 150
DQSLLMAGDT SSGKSEVRRL ALRALIDLSV APPGKKGSKL GVQIPSAEYI
160 170 180 190 200
LEALGNSRTL ENSNASRFGK YTELQFSDSG KLVGAKTLDY YLEKNRVVSA
210 220 230 240 250
ASSERNFHIF HYMVAGASDE EKQYLGIHDA ASFRYLGQAS RNMDTQDAAK
260 270 280 290 300
FDRLKLAFKN VGFSKRNVAS ICQVLAAILH LGNIEFHYDR QRTQDSATIR
310 320 330 340 350
NPEVLDKVAE YLGISSKSLE EALTYKTKMI RNEVCTILLD ADGASDHRDD
360 370 380 390 400
LAKSLYSLLF AWVNESLNEK LCRDDFDTFI GLLDLPGFQN LSKGNSLDQF
410 420 430 440 450
CVNFACENLH RFMLRSVFEK RRDEFADEGI SHLSPEVPYF DNAETLRLMT
460 470 480 490 500
NQPGGLIHIM DDQARRMPKK TDQTMIEAFG KRWGNHPSFK VGPADRSGFS
510 520 530 540 550
SFTISHYNSA VTYTSENLLE KNSEVVSTDF VSLLRGNPQE SGKLRNDSSQ
560 570 580 590 600
SSGSTIPFIR GIFNTKVLKT QSHPKNDQTI VAAQQSVKPM RAPSTRRPNR
610 620 630 640 650
GNTIKRTNTI KKADDDDSDE DAADAADAST SKKNAVRCVA GDFRGALDLL
660 670 680 690 700
LETLEDTKTW FTLCLRPNDN QLPNQFEARV VKQQITTLGL SEMSRKLLNE
710 720 730 740 750
YSVSMTYEEF CQRYADVPSL QAVQMRDAVS GEAKQKFSAA RQVMSWSDQE
760 770 780 790 800
AVSGRVKVFL SHTAFRELED ELRAADAEEV KNNEKRAQLD ADAAARGESD
810 820 830 840 850
PFSPVAVLAD DYTRSRSNDF VGAYGDPFKE RSSVALPLVG RGAAGNEDDL
860 870 880 890 900
EEVKSQYSGM SGTLARHSFV GGLSGAPSFV ASEAYAPSRN MFADMGKNGL
910 920 930 940 950
NEKAGTAAFT EEPLGEVAEE VGVSSTRRKW VALTWAITFW IPSFILSRFR
960 970 980 990 1000
SLKRPDIRMA WREKLAINLI IWFICACAVF VIVVLGNLIC PKQHVYSPTE
1010 1020 1030 1040 1050
FASHKGDSSF TAIRGEVFDL SNLVASHKTI VPVVPANSIL AYAGEDATPI
1060 1070 1080 1090 1100
FPVQVNALCN GVDGNVSPWV QLSNENSTDK HAQYHDFRSY HIDDARPDWY
1110 1120 1130 1140 1150
YESMWLLRSN YRVGFMGYTT DGIRDILSEG RAVAIYRGDI YDVSDYIKQG
1160 1170 1180 1190 1200
NQGVLRAPDG FQAPANTNRK FMSDAIISLI AQNPGKDITK QLDNLPLDPV
1210 1220 1230 1240 1250
VLDRQRVCLR NLYFIGKVDH RNSPQCRFAQ YILLALSLFM VAILGFKFLA
1260 1270 1280 1290 1300
ALQFGRARKP EDHDKFVICQ VPCYTEGEES MRKTINSLAA LKYDDKRKLL
1310 1320 1330 1340 1350
FIICDGMIVG SGNDRPTPRI VLDILGADPN LEPEALSFLS LGEGSKQHNM
1360 1370 1380 1390 1400
AKIYSGLYEH HGHVVPYIVV VKCGKPSERS RPGNRGKRDS QLVLMRFLNK
1410 1420 1430 1440 1450
VHFGLPMNPM ELEIYHQIKN VIGVNPSFYE YILQVDADTE VEAMSLNRFI
1460 1470 1480 1490 1500
SAFIRDKKVI GLCGETALSN AKASIITMLQ VYEYYISHYL AKAFESLFGS
1510 1520 1530 1540 1550
VTCLPGCFSM FRIRTPDTHR PLFIASQIVE DYAENRVDTL HTKNLLHLGE
1560 1570 1580 1590 1600
DRYLTTLVLK HFGKYKTIFV RDCKAWTVAP DDWKVLLSQR RRWINSTVHN
1610 1620 1630 1640 1650
LVELIFTPGL CGFCLFSMRF IVFIDLLSTI IAPVTVCYIV YLIVLVATAN
1660 1670 1680 1690 1700
GTVPLTAIIM LAAIYGCQAV IFLLNRKFEM IGWMIVYIIG IPIWSLFLPL
1710 1720 1730 1740 1750
YSFWHMDDFS WGNTRVVMGE KGQKVVLHEE GTFDPSEIPL QTWTDYENEL
1760 1770 1780 1790 1800
WERNSARSIG SIIEAARAEN KSLGSRAGSQ YAPSLYGQPM LPHNASFGHS
1810 1820 1830 1840 1850
PSPSYGGTPS QFGAFAPGPG SQIGGSQIGA GAGYFPQDAA RQSTYSIGGG
1860 1870 1880 1890 1900
YGGQAMSMYG LPPSSSFGVP TGGSGFMPQP FNTTASMYGY PQQVAATQSI
1910 1920 1930 1940 1950
YGGSQLGFGG GFATAEQQQQ QQQQQQAAGL SGSGGSKSPP REAVAGGLPS
1960 1970 1980 1990 2000
DSQIKLDIRS LIAESDLTTI TKKQLRAKLE QKYATSIESK KAFINSEIEN
VLSES
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CM003147 Genomic DNA Translation: KIS68630.1 |
RefSeqi | XP_011389642.1, XM_011391340.1 |
Genome annotation databases
EnsemblFungii | KIS68630; KIS68630; UMAG_03204 |
GeneIDi | 23563730 |
KEGGi | uma:UMAG_03204 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CM003147 Genomic DNA Translation: KIS68630.1 |
RefSeqi | XP_011389642.1, XM_011391340.1 |
3D structure databases
SMRi | Q4P9K9 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | Q4P9K9, 1 interactor |
MINTi | Q4P9K9 |
STRINGi | 5270.UM03204P0 |
Proteomic databases
PRIDEi | Q4P9K9 |
Genome annotation databases
EnsemblFungii | KIS68630; KIS68630; UMAG_03204 |
GeneIDi | 23563730 |
KEGGi | uma:UMAG_03204 |
Organism-specific databases
VEuPathDBi | FungiDB:UMAG_03204 |
Phylogenomic databases
eggNOGi | KOG2571, Eukaryota KOG4229, Eukaryota |
HOGENOMi | CLU_000192_0_2_1 |
InParanoidi | Q4P9K9 |
OMAi | MRYDDKR |
OrthoDBi | 20724at2759 |
Enzyme and pathway databases
BRENDAi | 2.4.1.16, 6587 |
Miscellaneous databases
PHI-basei | PHI:1114 PHI:2226 |
Family and domain databases
CDDi | cd14879, MYSc_Myo17, 1 hit |
Gene3Di | 3.40.850.10, 2 hits |
InterProi | View protein in InterPro IPR004835, Chitin_synth IPR001199, Cyt_B5-like_heme/steroid-bd IPR036400, Cyt_B5-like_heme/steroid_sf IPR014876, DEK_C IPR036961, Kinesin_motor_dom_sf IPR001609, Myosin_head_motor_dom IPR036037, MYSc_Myo17 IPR029044, Nucleotide-diphossugar_trans IPR027417, P-loop_NTPase |
PANTHERi | PTHR22914, PTHR22914, 1 hit |
Pfami | View protein in Pfam PF00173, Cyt-b5, 1 hit PF08766, DEK_C, 1 hit PF00063, Myosin_head, 1 hit |
PRINTSi | PR00193, MYOSINHEAVY |
SMARTi | View protein in SMART SM01117, Cyt-b5, 2 hits SM00242, MYSc, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit SSF53448, SSF53448, 1 hit SSF55856, SSF55856, 1 hit |
PROSITEi | View protein in PROSITE PS51456, MYOSIN_MOTOR, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CHS8_USTMA | |
Accessioni | Q4P9K9Primary (citable) accession number: Q4P9K9 Secondary accession number(s): A0A0D1E236 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 9, 2007 |
Last sequence update: | July 19, 2005 | |
Last modified: | February 23, 2022 | |
This is version 103 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families