UniProtKB - Q4LDG0 (S27A5_MOUSE)
Protein
Bile acyl-CoA synthetase
Gene
Slc27a5
Organism
Mus musculus (Mouse)
Status
Functioni
Acyl-CoA synthetase that catalyzes the activation of bile acids via formation of bile acid CoA thioesters which is necessary for their subsequent conjugation with glycine or taurine (PubMed:16618417). Catalyzes the activation of the primary bile acid, cholic acid (PubMed:16618417). Both primary bile acid (chenodeoxycholic acid) and secondary bile acids (deoxycholic acid and lithocholic acid) are the principal substrates (By similarity). Also exhibits acyl CoA synthetase activity that activates very long-chain fatty acids (VLCFAs) by catalyzing the formation of fatty acyl-CoA (By similarity). In vitro, also activates 3-alpha,7-alpha,12-alpha-trihydroxy-5- beta-cholestanate (THCA), the C27 precursor of cholic acid deriving from the de novo synthesis from cholesterol (By similarity). Exhibits long-chain fatty acids (LCFA) transport activity (PubMed:16618416). Plays an important role in hepatic fatty acid uptake and bile acid reconjugation and recycling but not in de novo synthesis of bile acids (PubMed:16618416, PubMed:16618417).By similarity2 Publications
Catalytic activityi
- EC:6.2.1.71 PublicationThis reaction proceeds in the forward1 Publication direction.
- (25R)-3α,7α,12α-trihydroxy-5β-cholestan-26-oate + ATP + CoA = (25R)-3α,7α,12α-trihydroxy-5β-cholestan-26-oyl-CoA + AMP + diphosphateBy similarityEC:6.2.1.7By similarityThis reaction proceeds in the forwardBy similarity direction.
- a very long-chain fatty acid + ATP + CoA = a very long-chain fatty acyl-CoA + AMP + diphosphateBy similarityThis reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the backward1 Publication direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 292 – 303 | AMPSequence analysisAdd BLAST | 12 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- cholate-CoA ligase activity Source: UniProtKB
- fatty acid transmembrane transporter activity Source: MGI
- long-chain fatty acid-CoA ligase activity Source: GO_Central
- long-chain fatty acid transporter activity Source: MGI
- protein-containing complex binding Source: MGI
- very long-chain fatty acid-CoA ligase activity Source: MGI
GO - Biological processi
- bile acid biosynthetic process Source: MGI
- bile acid metabolic process Source: MGI
- fatty acid transport Source: MGI
- ketone body biosynthetic process Source: MGI
- long-chain fatty acid import across plasma membrane Source: MGI
- long-chain fatty acid metabolic process Source: MGI
- triglyceride mobilization Source: MGI
- very long-chain fatty acid metabolic process Source: MGI
Keywordsi
Molecular function | Ligase |
Biological process | Fatty acid metabolism, Lipid metabolism |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-MMU-159418, Recycling of bile acids and salts R-MMU-193368, Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol R-MMU-193775, Synthesis of bile acids and bile salts via 24-hydroxycholesterol |
Protein family/group databases
TCDBi | 4.C.1.1.8, the fatty acid transporter (fat) family |
Names & Taxonomyi
Protein namesi | Recommended name: Bile acyl-CoA synthetaseBy similarity (EC:6.2.1.71 Publication)Short name: BACSBy similarity Alternative name(s): Bile acid-CoA ligase1 Publication Short name: BA-CoA ligase1 Publication Short name: BAL1 Publication Cholate--CoA ligase1 Publication Fatty acid transport protein 52 Publications Short name: FATP-52 Publications Solute carrier family 27 member 5 Very long-chain acyl-CoA synthetase-related protein1 Publication Short name: VLACS-related1 Publication Short name: VLACSR1 Publication |
Gene namesi | Name:Slc27a5 Synonyms:Acsb, Acsvl6, Fatp5, Vlacsr |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1347100, Slc27a5 |
Subcellular locationi
Plasma membrane
- Cell membrane 1 Publication; Multi-pass membrane protein Sequence analysis
Endoplasmic reticulum
- Endoplasmic reticulum membrane By similarity; Multi-pass membrane protein Sequence analysis
- Microsome By similarity
Endoplasmic reticulum
- endoplasmic reticulum Source: MGI
- integral component of endoplasmic reticulum membrane Source: MGI
Plasma Membrane
- basal plasma membrane Source: MGI
Other locations
- intracellular membrane-bounded organelle Source: MGI
- protein-containing complex Source: MGI
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 29 | CytoplasmicBy similarityAdd BLAST | 29 | |
Transmembranei | 30 – 50 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 55 – 75 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 76 – 689 | CytoplasmicBy similarityAdd BLAST | 614 |
Keywords - Cellular componenti
Cell membrane, Endoplasmic reticulum, Membrane, MicrosomePathology & Biotechi
Disruption phenotypei
Mice exhibit a severe bile acid conjugation defect (PubMed:16618417). Display a significant reduction in both liver lipid uptake and content and show a redistribution of lipids away from the liver to other tissues (PubMed:16618416). Hepatocytes show significantly reduced long-chain fatty acids (LCFA) uptake (PubMed:16618416).2 Publications
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000193214 | 1 – 689 | Bile acyl-CoA synthetaseAdd BLAST | 689 |
Proteomic databases
jPOSTi | Q4LDG0 |
MaxQBi | Q4LDG0 |
PaxDbi | Q4LDG0 |
PeptideAtlasi | Q4LDG0 |
PRIDEi | Q4LDG0 |
PTM databases
iPTMneti | Q4LDG0 |
PhosphoSitePlusi | Q4LDG0 |
SwissPalmi | Q4LDG0 |
Expressioni
Tissue specificityi
Liver-specific (at protein level) (PubMed:16618416, PubMed:9642112). In liver expressed in a periportal distribution (PubMed:11980911).3 Publications
Gene expression databases
Bgeei | ENSMUSG00000030382, Expressed in liver and 78 other tissues |
Genevisiblei | Q4LDG0, MM |
Interactioni
Protein-protein interaction databases
IntActi | Q4LDG0, 2 interactors |
MINTi | Q4LDG0 |
STRINGi | 10090.ENSMUSP00000032539 |
Miscellaneous databases
RNActi | Q4LDG0, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the ATP-dependent AMP-binding enzyme family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG1179, Eukaryota |
GeneTreei | ENSGT00940000157947 |
HOGENOMi | CLU_000022_46_2_1 |
InParanoidi | Q4LDG0 |
OMAi | GATFFTY |
OrthoDBi | 298283at2759 |
PhylomeDBi | Q4LDG0 |
TreeFami | TF313430 |
Family and domain databases
Gene3Di | 3.40.50.12780, 1 hit |
InterProi | View protein in InterPro IPR025110, AMP-bd_C IPR020845, AMP-binding_CS IPR000873, AMP-dep_Synth/Lig IPR042099, AMP-dep_Synthh-like_sf IPR030302, FATP5 |
PANTHERi | PTHR43107:SF4, PTHR43107:SF4, 1 hit |
Pfami | View protein in Pfam PF00501, AMP-binding, 1 hit PF13193, AMP-binding_C, 1 hit |
PROSITEi | View protein in PROSITE PS00455, AMP_BINDING, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
Q4LDG0-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGIWKKLTLL LLLLLLVGLG QPPWPAAMAL ALRWFLGDPT CLVLLGLALL
60 70 80 90 100
GRPWISSWMP HWLSLVGAAL TLFLLPLQPP PGLRWLHKDV AFTFKMLFYG
110 120 130 140 150
LKFRRRLNKH PPETFVDALE RQALAWPDRV ALVCTGSEGS SITNSQLDAR
160 170 180 190 200
SCQAAWVLKA KLKDAVIQNT RDAAAILVLP SKTISALSVF LGLAKLGCPV
210 220 230 240 250
AWINPHSRGM PLLHSVRSSG ASVLIVDPDL QENLEEVLPK LLAENIHCFY
260 270 280 290 300
LGHSSPTPGV EALGASLDAA PSDPVPASLR ATIKWKSPAI FIFTSGTTGL
310 320 330 340 350
PKPAILSHER VIQVSNVLSF CGCRADDVVY DVLPLYHTIG LVLGFLGCLQ
360 370 380 390 400
VGATCVLAPK FSASRFWAEC RQHGVTVILY VGEILRYLCN VPEQPEDKIH
410 420 430 440 450
TVRLAMGNGL RANVWKNFQQ RFGPIRIWEF YGSTEGNVGL MNYVGHCGAV
460 470 480 490 500
GRTSCILRML TPFELVQFDI ETAEPLRDKQ GFCIPVEPGK PGLLLTKVRK
510 520 530 540 550
NQPFLGYRGS QAESNRKLVA NVRRVGDLYF NTGDVLTLDQ EGFFYFQDRL
560 570 580 590 600
GDTFRWKGEN VSTGEVECVL SSLDFLEEVN VYGVPVPGCE GKVGMAAVKL
610 620 630 640 650
APGKTFDGQK LYQHVRSWLP AYATPHFIRI QDSLEITNTY KLVKSRLVRE
660 670 680
GFDVGIIADP LYILDNKAQT FRSLMPDVYQ AVCEGTWNL
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE9PXV4 | E9PXV4_MOUSE | Bile acyl-CoA synthetase | Slc27a5 | 418 | Annotation score: | ||
F6YDR6 | F6YDR6_MOUSE | Bile acyl-CoA synthetase | Slc27a5 | 195 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 88 | K → I in CAA11688 (PubMed:9642112).Curated | 1 | |
Sequence conflicti | 408 | N → T in AAC40189 (PubMed:9671728).Curated | 1 | |
Sequence conflicti | 568 | C → S in CAA11688 (PubMed:9642112).Curated | 1 | |
Sequence conflicti | 688 | N → K in CAA11688 (PubMed:9642112).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ223959 mRNA Translation: CAA11688.1 BC013335 mRNA Translation: AAH13335.1 BC013272 mRNA Translation: AAH13272.1 BC145823 mRNA Translation: AAI45824.1 BC145825 mRNA Translation: AAI45826.1 AF072760 mRNA Translation: AAC40189.1 |
CCDSi | CCDS20821.1 |
PIRi | JW0107 |
RefSeqi | NP_033538.2, NM_009512.2 |
Genome annotation databases
Ensembli | ENSMUST00000032539; ENSMUSP00000032539; ENSMUSG00000030382 |
GeneIDi | 26459 |
KEGGi | mmu:26459 |
UCSCi | uc009fey.3, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ223959 mRNA Translation: CAA11688.1 BC013335 mRNA Translation: AAH13335.1 BC013272 mRNA Translation: AAH13272.1 BC145823 mRNA Translation: AAI45824.1 BC145825 mRNA Translation: AAI45826.1 AF072760 mRNA Translation: AAC40189.1 |
CCDSi | CCDS20821.1 |
PIRi | JW0107 |
RefSeqi | NP_033538.2, NM_009512.2 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
IntActi | Q4LDG0, 2 interactors |
MINTi | Q4LDG0 |
STRINGi | 10090.ENSMUSP00000032539 |
Protein family/group databases
TCDBi | 4.C.1.1.8, the fatty acid transporter (fat) family |
PTM databases
iPTMneti | Q4LDG0 |
PhosphoSitePlusi | Q4LDG0 |
SwissPalmi | Q4LDG0 |
Proteomic databases
jPOSTi | Q4LDG0 |
MaxQBi | Q4LDG0 |
PaxDbi | Q4LDG0 |
PeptideAtlasi | Q4LDG0 |
PRIDEi | Q4LDG0 |
Protocols and materials databases
Antibodypediai | 1943, 294 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000032539; ENSMUSP00000032539; ENSMUSG00000030382 |
GeneIDi | 26459 |
KEGGi | mmu:26459 |
UCSCi | uc009fey.3, mouse |
Organism-specific databases
CTDi | 10998 |
MGIi | MGI:1347100, Slc27a5 |
Phylogenomic databases
eggNOGi | KOG1179, Eukaryota |
GeneTreei | ENSGT00940000157947 |
HOGENOMi | CLU_000022_46_2_1 |
InParanoidi | Q4LDG0 |
OMAi | GATFFTY |
OrthoDBi | 298283at2759 |
PhylomeDBi | Q4LDG0 |
TreeFami | TF313430 |
Enzyme and pathway databases
Reactomei | R-MMU-159418, Recycling of bile acids and salts R-MMU-193368, Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol R-MMU-193775, Synthesis of bile acids and bile salts via 24-hydroxycholesterol |
Miscellaneous databases
BioGRID-ORCSi | 26459, 1 hit in 17 CRISPR screens |
ChiTaRSi | Slc27a5, mouse |
PROi | PR:Q4LDG0 |
RNActi | Q4LDG0, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000030382, Expressed in liver and 78 other tissues |
Genevisiblei | Q4LDG0, MM |
Family and domain databases
Gene3Di | 3.40.50.12780, 1 hit |
InterProi | View protein in InterPro IPR025110, AMP-bd_C IPR020845, AMP-binding_CS IPR000873, AMP-dep_Synth/Lig IPR042099, AMP-dep_Synthh-like_sf IPR030302, FATP5 |
PANTHERi | PTHR43107:SF4, PTHR43107:SF4, 1 hit |
Pfami | View protein in Pfam PF00501, AMP-binding, 1 hit PF13193, AMP-binding_C, 1 hit |
PROSITEi | View protein in PROSITE PS00455, AMP_BINDING, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | S27A5_MOUSE | |
Accessioni | Q4LDG0Primary (citable) accession number: Q4LDG0 Secondary accession number(s): A6H6C1, O88694, Q91VD5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 22, 2005 |
Last sequence update: | November 22, 2005 | |
Last modified: | December 2, 2020 | |
This is version 128 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families