UniProtKB - Q4LDD4 (ARAP1_MOUSE)
Protein
Arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 1
Gene
Arap1
Organism
Mus musculus (Mouse)
Status
Functioni
Phosphatidylinositol 3,4,5-trisphosphate-dependent GTPase-activating protein that modulates actin cytoskeleton remodeling by regulating ARF and RHO family members. Is activated by phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3) binding. Can be activated by phosphatidylinositol 3,4-bisphosphate (PtdIns(3,4,5)P2) binding, albeit with lower efficiency. Has a preference for ARF1 and ARF5 (By similarity).By similarity
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 552 – 575 | C4-typePROSITE-ProRule annotationAdd BLAST | 24 |
GO - Molecular functioni
- DNA-binding transcription factor activity Source: UniProtKB
- GTPase activator activity Source: UniProtKB
- metal ion binding Source: UniProtKB-KW
- phosphatidylinositol-3,4,5-trisphosphate binding Source: MGI
- R-SMAD binding Source: BHF-UCL
- transcription factor binding Source: BHF-UCL
- transcription regulatory region sequence-specific DNA binding Source: UniProtKB
- type 1 angiotensin receptor binding Source: MGI
GO - Biological processi
- negative regulation of stress fiber assembly Source: MGI
- positive regulation of filopodium assembly Source: MGI
- positive regulation of GTPase activity Source: MGI
- positive regulation of receptor recycling Source: MGI
- positive regulation of transcription, DNA-templated Source: UniProtKB
- regulation of cell shape Source: MGI
- regulation of cellular component movement Source: MGI
- SMAD protein signal transduction Source: UniProtKB
- transforming growth factor beta receptor signaling pathway Source: UniProtKB
Keywordsi
Molecular function | GTPase activation |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-MMU-194840, Rho GTPase cycle R-MMU-8849469, PTK6 Regulates RTKs and Their Effectors AKT1 and DOK1 |
Names & Taxonomyi
Protein namesi | Recommended name: Arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 1Alternative name(s): Centaurin-delta-2By similarity Short name: Cnt-d2By similarity |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1916960, Arap1 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm By similarity
Golgi apparatus
- Golgi stack membrane By similarity; Peripheral membrane protein By similarity
Plasma membrane
- Cell membrane By similarity
Cytosol
- cytosol Source: MGI
Golgi apparatus
- Golgi apparatus Source: MGI
- Golgi cisterna membrane Source: UniProtKB-SubCell
- trans-Golgi network Source: MGI
Nucleus
- nucleoplasm Source: MGI
Plasma Membrane
- plasma membrane Source: MGI
Other locations
- cytoplasmic vesicle Source: MGI
- intracellular membrane-bounded organelle Source: MGI
Keywords - Cellular componenti
Cell membrane, Cytoplasm, Golgi apparatus, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000367029 | 1 – 1452 | Arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 1Add BLAST | 1452 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 232 | PhosphoserineBy similarity | 1 | |
Modified residuei | 430 | PhosphoserineBy similarity | 1 | |
Modified residuei | 506 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 740 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1430 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1437 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
jPOSTi | Q4LDD4 |
PaxDbi | Q4LDD4 |
PeptideAtlasi | Q4LDD4 |
PRIDEi | Q4LDD4 |
ProteomicsDBi | 296277 [Q4LDD4-1] 296278 [Q4LDD4-2] 296279 [Q4LDD4-3] |
PTM databases
iPTMneti | Q4LDD4 |
PhosphoSitePlusi | Q4LDD4 |
SwissPalmi | Q4LDD4 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000032812, Expressed in bone marrow macrophage and 193 other tissues |
Genevisiblei | Q4LDD4, MM |
Interactioni
Subunit structurei
Interacts with TNFRSF10A.
By similarityGO - Molecular functioni
- R-SMAD binding Source: BHF-UCL
- transcription factor binding Source: BHF-UCL
- type 1 angiotensin receptor binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 213630, 1 interactor |
IntActi | Q4LDD4, 1 interactor |
STRINGi | 10090.ENSMUSP00000102624 |
Miscellaneous databases
RNActi | Q4LDD4, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 6 – 70 | SAMPROSITE-ProRule annotationAdd BLAST | 65 | |
Domaini | 329 – 421 | PH 1PROSITE-ProRule annotationAdd BLAST | 93 | |
Domaini | 442 – 531 | PH 2PROSITE-ProRule annotationAdd BLAST | 90 | |
Domaini | 537 – 662 | Arf-GAPPROSITE-ProRule annotationAdd BLAST | 126 | |
Domaini | 745 – 852 | PH 3PROSITE-ProRule annotationAdd BLAST | 108 | |
Domaini | 956 – 1141 | Rho-GAPPROSITE-ProRule annotationAdd BLAST | 186 | |
Domaini | 1174 – 1263 | Ras-associatingPROSITE-ProRule annotationAdd BLAST | 90 | |
Domaini | 1276 – 1398 | PH 4PROSITE-ProRule annotationAdd BLAST | 123 |
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 552 – 575 | C4-typePROSITE-ProRule annotationAdd BLAST | 24 |
Keywords - Domaini
Repeat, Zinc-fingerPhylogenomic databases
eggNOGi | KOG1117, Eukaryota |
GeneTreei | ENSGT00940000157424 |
HOGENOMi | CLU_002900_1_1_1 |
InParanoidi | Q4LDD4 |
OrthoDBi | 98944at2759 |
PhylomeDBi | Q4LDD4 |
TreeFami | TF105769 |
Family and domain databases
CDDi | cd04385, RhoGAP_ARAP, 1 hit |
Gene3Di | 1.10.150.50, 1 hit 1.10.555.10, 1 hit 2.30.29.30, 4 hits 3.30.40.160, 1 hit |
InterProi | View protein in InterPro IPR037278, ARFGAP/RecO IPR001164, ArfGAP_dom IPR038508, ArfGAP_dom_sf IPR011993, PH-like_dom_sf IPR001849, PH_domain IPR000159, RA_dom IPR008936, Rho_GTPase_activation_prot IPR037858, RhoGAP_ARAP IPR000198, RhoGAP_dom IPR001660, SAM IPR013761, SAM/pointed_sf |
Pfami | View protein in Pfam PF01412, ArfGap, 1 hit PF00169, PH, 3 hits PF00788, RA, 1 hit PF00620, RhoGAP, 1 hit PF07647, SAM_2, 1 hit |
PRINTSi | PR00405, REVINTRACTNG |
SMARTi | View protein in SMART SM00105, ArfGap, 1 hit SM00233, PH, 5 hits SM00324, RhoGAP, 1 hit SM00454, SAM, 1 hit |
SUPFAMi | SSF47769, SSF47769, 1 hit SSF48350, SSF48350, 1 hit SSF57863, SSF57863, 1 hit |
PROSITEi | View protein in PROSITE PS50115, ARFGAP, 1 hit PS50003, PH_DOMAIN, 4 hits PS50200, RA, 1 hit PS50238, RHOGAP, 1 hit PS50105, SAM_DOMAIN, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 9 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q4LDD4-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAEGYDAALS VAEWLRALHL EQYTALFEQH GLVWATECQG LSDAGLLDMG
60 70 80 90 100
MHLPGHRRRI LAGLHRAHAP PVPLPRPAPR PVPMKRHIFR SPPVPVTPPE
110 120 130 140 150
PPPTAGEDEG LPAAPPIPPR RSCLPPACFT PTSTAAPDPV LPPLPAKRHL
160 170 180 190 200
VEPSVPPVPP RTGPPYPQAS LLAKEELLLP SVSPRSQPEP AETPSTLLPA
210 220 230 240 250
FPQGPLQPPS PPPCPPVIPP KPPRLLPEFD DSDYDDVPEE GPGAPASVMT
260 270 280 290 300
KEEPLPSRVP RAVRVASLLS EGEELSGDDS EDDDDHAYEG IPNGGWPTSG
310 320 330 340 350
LNPPLRSLIP DLPLHPMDEL PGGPTPITPV IKAGWLDKNP PQGSYIYQKR
360 370 380 390 400
WVRLDADYLR YFDSNKDAYS KRFVPVACIC RVAPIGDQKF EVITNNRTFA
410 420 430 440 450
FRAESDVERN EWMQALQQAV VEHRARFRLS SASVLGVRGS EQPDRAGSLE
460 470 480 490 500
LRGFKNKLYV AVTGDKVQLY KNLEEFHLGI GITFIDMNVG NVKEVDRRSF
510 520 530 540 550
DLTTPYRIFS FSADSELEKE QWLEAMQGAI AEALSTSEVA ERIWAAAPNR
560 570 580 590 600
FCADCGAAQP DWASINLCVV ICKRCAGEHR GLGAGVSKVR SLKMDRKVWT
610 620 630 640 650
EALIQLFLHL GNGPGNHFWA ANVPPSEALE PSSSPGARRY HLEAKYREGK
660 670 680 690 700
YRRYHPLFGN QEELDKALCA AVTTTDLAET QALLGCGAGV SCFSGDPAAP
710 720 730 740 750
TPLALAEQAG QTLQMEFLRN NQSTEVPRLD SVKPLEKHYS VTLPTVSHSG
760 770 780 790 800
FLYKTASAGK PLQDRRAREE FSRRWCVLSD GVLSYYENER AVTPNGEIRA
810 820 830 840 850
SEIVCLAVSP LDTHGFEHTF EVYTEGERLY LFGLENAELA HEWVKCIAKA
860 870 880 890 900
FVPPLAEDLL ARDFERLGRL PCKAGLSLQQ AQEGWFALTG SELRAVFPEG
910 920 930 940 950
PWEEPLQLRK LQELSIQGDS ENQVLVLVER RRTLYIQGER RLDFMAWLGV
960 970 980 990 1000
IQKAAASLGD TLSEQQLGDS DIPVIVYRCV DYITQCGLTS EGIYRKCGQT
1010 1020 1030 1040 1050
SKTQRLLDSL RQDARSVHLK EGEQHVDDVS SALKRFLRDL PDGLFTRAQR
1060 1070 1080 1090 1100
LAWLEASEIE DEEEKISRYR ELLVHLPPVN RATVKALISH LYCVQCFSDT
1110 1120 1130 1140 1150
NQMNTHNLAI VFGPTLFQTD GQDYKAGKVV EDLINHYVVV FSVDEEELRK
1160 1170 1180 1190 1200
QREEVTAIVK MRVAGTASGT QHAGDFICTV YLEEKKVETE QHVKIPASMT
1210 1220 1230 1240 1250
AEELTLEILD RRNVSIREKD YWTCFEVNEK EEAERPLHFA EKVLPIVHGL
1260 1270 1280 1290 1300
GIDSHLVVKK YQSMEAMLLY LASRVGDTKH GMMKFREDRS LLGLGLPSGG
1310 1320 1330 1340 1350
FHDRYFILNS SCLRLYKEVR SQRPWSGAPE TSHRPEKEWP VKSLKVYLGV
1360 1370 1380 1390 1400
KKKLRPPTCW GFTVVHETEK HEKQQWYLCC DTQMELREWF ATFLSVQHDG
1410 1420 1430 1440 1450
LVWPSEPSRV SRAVPEVRMG SVSLIPLRGS ENEMRRSVAA FTADPLSLLR
HV
Computationally mapped potential isoform sequencesi
There are 9 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE9PUB0 | E9PUB0_MOUSE | Arf-GAP with Rho-GAP domain, ANK re... | Arap1 | 727 | Annotation score: | ||
D3YWW7 | D3YWW7_MOUSE | Arf-GAP with Rho-GAP domain, ANK re... | Arap1 | 111 | Annotation score: | ||
D3YY40 | D3YY40_MOUSE | Arf-GAP with Rho-GAP domain, ANK re... | Arap1 | 63 | Annotation score: | ||
D3Z2X3 | D3Z2X3_MOUSE | Arf-GAP with Rho-GAP domain, ANK re... | Arap1 | 76 | Annotation score: | ||
A0A1B0GSK4 | A0A1B0GSK4_MOUSE | Arf-GAP with Rho-GAP domain, ANK re... | Arap1 | 34 | Annotation score: | ||
D3Z2S2 | D3Z2S2_MOUSE | Arf-GAP with Rho-GAP domain, ANK re... | Arap1 | 57 | Annotation score: | ||
D3YZJ7 | D3YZJ7_MOUSE | Arf-GAP with Rho-GAP domain, ANK re... | Arap1 | 54 | Annotation score: | ||
D3YYF5 | D3YYF5_MOUSE | Arf-GAP with Rho-GAP domain, ANK re... | Arap1 | 19 | Annotation score: | ||
D3YVS8 | D3YVS8_MOUSE | Arf-GAP with Rho-GAP domain, ANK re... | Arap1 | 12 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 153 | P → S in AAI41180 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 280 | S → A in AAI41180 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 924 | V → G in CAF21318 (Ref. 1) Curated | 1 | |
Sequence conflicti | 1331 | T → R in AAH57922 (PubMed:15489334).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_053047 | 1 – 248 | Missing in isoform 3. 1 PublicationAdd BLAST | 248 | |
Alternative sequenceiVSP_053048 | 1322 – 1332 | Missing in isoform 2 and isoform 3. 2 PublicationsAdd BLAST | 11 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ621558 mRNA Translation: CAF21318.1 AK137455 mRNA Translation: BAE23358.1 AK140646 mRNA Translation: BAE24434.1 AK150132 mRNA Translation: BAE29329.1 BC057922 mRNA Translation: AAH57922.1 BC091762 mRNA Translation: AAH91762.1 BC141179 mRNA Translation: AAI41180.1 AK129213 mRNA Translation: BAC98023.1 |
CCDSi | CCDS21510.1 [Q4LDD4-3] CCDS40041.1 [Q4LDD4-2] |
RefSeqi | NP_001035200.1, NM_001040111.1 [Q4LDD4-2] NP_001035201.1, NM_001040112.1 [Q4LDD4-3] NP_081456.2, NM_027180.3 [Q4LDD4-3] NP_932764.1, NM_198096.1 XP_006508249.1, XM_006508186.3 [Q4LDD4-1] XP_006508250.1, XM_006508187.1 [Q4LDD4-1] XP_006508251.1, XM_006508188.3 [Q4LDD4-1] XP_011240199.1, XM_011241897.2 [Q4LDD4-1] |
Genome annotation databases
Ensembli | ENSMUST00000084895; ENSMUSP00000081957; ENSMUSG00000032812 [Q4LDD4-3] ENSMUST00000084896; ENSMUSP00000081958; ENSMUSG00000032812 [Q4LDD4-1] ENSMUST00000107010; ENSMUSP00000102624; ENSMUSG00000032812 [Q4LDD4-2] |
GeneIDi | 69710 |
KEGGi | mmu:69710 |
UCSCi | uc009iok.1, mouse [Q4LDD4-2] uc009iol.1, mouse [Q4LDD4-1] uc009iom.1, mouse [Q4LDD4-3] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ621558 mRNA Translation: CAF21318.1 AK137455 mRNA Translation: BAE23358.1 AK140646 mRNA Translation: BAE24434.1 AK150132 mRNA Translation: BAE29329.1 BC057922 mRNA Translation: AAH57922.1 BC091762 mRNA Translation: AAH91762.1 BC141179 mRNA Translation: AAI41180.1 AK129213 mRNA Translation: BAC98023.1 |
CCDSi | CCDS21510.1 [Q4LDD4-3] CCDS40041.1 [Q4LDD4-2] |
RefSeqi | NP_001035200.1, NM_001040111.1 [Q4LDD4-2] NP_001035201.1, NM_001040112.1 [Q4LDD4-3] NP_081456.2, NM_027180.3 [Q4LDD4-3] NP_932764.1, NM_198096.1 XP_006508249.1, XM_006508186.3 [Q4LDD4-1] XP_006508250.1, XM_006508187.1 [Q4LDD4-1] XP_006508251.1, XM_006508188.3 [Q4LDD4-1] XP_011240199.1, XM_011241897.2 [Q4LDD4-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5XHZ | X-ray | 1.32 | C/D | 80-90 | [»] | |
SMRi | Q4LDD4 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 213630, 1 interactor |
IntActi | Q4LDD4, 1 interactor |
STRINGi | 10090.ENSMUSP00000102624 |
PTM databases
iPTMneti | Q4LDD4 |
PhosphoSitePlusi | Q4LDD4 |
SwissPalmi | Q4LDD4 |
Proteomic databases
jPOSTi | Q4LDD4 |
PaxDbi | Q4LDD4 |
PeptideAtlasi | Q4LDD4 |
PRIDEi | Q4LDD4 |
ProteomicsDBi | 296277 [Q4LDD4-1] 296278 [Q4LDD4-2] 296279 [Q4LDD4-3] |
Protocols and materials databases
Antibodypediai | 30876, 172 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000084895; ENSMUSP00000081957; ENSMUSG00000032812 [Q4LDD4-3] ENSMUST00000084896; ENSMUSP00000081958; ENSMUSG00000032812 [Q4LDD4-1] ENSMUST00000107010; ENSMUSP00000102624; ENSMUSG00000032812 [Q4LDD4-2] |
GeneIDi | 69710 |
KEGGi | mmu:69710 |
UCSCi | uc009iok.1, mouse [Q4LDD4-2] uc009iol.1, mouse [Q4LDD4-1] uc009iom.1, mouse [Q4LDD4-3] |
Organism-specific databases
CTDi | 116985 |
MGIi | MGI:1916960, Arap1 |
Rougei | Search... |
Phylogenomic databases
eggNOGi | KOG1117, Eukaryota |
GeneTreei | ENSGT00940000157424 |
HOGENOMi | CLU_002900_1_1_1 |
InParanoidi | Q4LDD4 |
OrthoDBi | 98944at2759 |
PhylomeDBi | Q4LDD4 |
TreeFami | TF105769 |
Enzyme and pathway databases
Reactomei | R-MMU-194840, Rho GTPase cycle R-MMU-8849469, PTK6 Regulates RTKs and Their Effectors AKT1 and DOK1 |
Miscellaneous databases
BioGRID-ORCSi | 69710, 1 hit in 52 CRISPR screens |
ChiTaRSi | Arap1, mouse |
PROi | PR:Q4LDD4 |
RNActi | Q4LDD4, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000032812, Expressed in bone marrow macrophage and 193 other tissues |
Genevisiblei | Q4LDD4, MM |
Family and domain databases
CDDi | cd04385, RhoGAP_ARAP, 1 hit |
Gene3Di | 1.10.150.50, 1 hit 1.10.555.10, 1 hit 2.30.29.30, 4 hits 3.30.40.160, 1 hit |
InterProi | View protein in InterPro IPR037278, ARFGAP/RecO IPR001164, ArfGAP_dom IPR038508, ArfGAP_dom_sf IPR011993, PH-like_dom_sf IPR001849, PH_domain IPR000159, RA_dom IPR008936, Rho_GTPase_activation_prot IPR037858, RhoGAP_ARAP IPR000198, RhoGAP_dom IPR001660, SAM IPR013761, SAM/pointed_sf |
Pfami | View protein in Pfam PF01412, ArfGap, 1 hit PF00169, PH, 3 hits PF00788, RA, 1 hit PF00620, RhoGAP, 1 hit PF07647, SAM_2, 1 hit |
PRINTSi | PR00405, REVINTRACTNG |
SMARTi | View protein in SMART SM00105, ArfGap, 1 hit SM00233, PH, 5 hits SM00324, RhoGAP, 1 hit SM00454, SAM, 1 hit |
SUPFAMi | SSF47769, SSF47769, 1 hit SSF48350, SSF48350, 1 hit SSF57863, SSF57863, 1 hit |
PROSITEi | View protein in PROSITE PS50115, ARFGAP, 1 hit PS50003, PH_DOMAIN, 4 hits PS50200, RA, 1 hit PS50238, RHOGAP, 1 hit PS50105, SAM_DOMAIN, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ARAP1_MOUSE | |
Accessioni | Q4LDD4Primary (citable) accession number: Q4LDD4 Secondary accession number(s): B2RUJ3 Q6ZQ48 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 24, 2009 |
Last sequence update: | March 24, 2009 | |
Last modified: | April 7, 2021 | |
This is version 140 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references