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UniProtKB - Q4LB35 (FOL1_SCHPO)
Protein
Folic acid synthesis protein fol1
Gene
fol1
Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Functioni
Catalyzes three sequential steps of tetrahydrofolate biosynthesis.
By similarityCatalytic activityi
- EC:4.1.2.25By similarity
- 6-hydroxymethyl-7,8-dihydropterin + ATP = (7,8-dihydropterin-6-yl)methyl diphosphate + AMP + H+By similarityEC:2.7.6.3By similarity
- (7,8-dihydropterin-6-yl)methyl diphosphate + 4-aminobenzoate = 7,8-dihydropteroate + diphosphateBy similarityEC:2.5.1.15By similarity
Cofactori
Mg2+By similarity
: tetrahydrofolate biosynthesis Pathwayi
This protein is involved in step 3 and 4 of the subpathway that synthesizes 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate from 7,8-dihydroneopterin triphosphate. This subpathway is part of the pathway tetrahydrofolate biosynthesis, which is itself part of Cofactor biosynthesis.View all proteins of this organism that are known to be involved in the subpathway that synthesizes 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate from 7,8-dihydroneopterin triphosphate, the pathway tetrahydrofolate biosynthesis and in Cofactor biosynthesis.
Pathwayi: tetrahydrofolate biosynthesis
This protein is involved in step 1 of the subpathway that synthesizes 7,8-dihydrofolate from 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate and 4-aminobenzoate. This subpathway is part of the pathway tetrahydrofolate biosynthesis, which is itself part of Cofactor biosynthesis.View all proteins of this organism that are known to be involved in the subpathway that synthesizes 7,8-dihydrofolate from 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate and 4-aminobenzoate, the pathway tetrahydrofolate biosynthesis and in Cofactor biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 472 | MagnesiumBy similarity | 1 | |
Binding sitei | 511 | 6-hydroxymethyl-7,8-dihydropterin diphosphateBy similarity | 1 | |
Binding sitei | 546 | 6-hydroxymethyl-7,8-dihydropterin diphosphateBy similarity | 1 | |
Binding sitei | 565 | 6-hydroxymethyl-7,8-dihydropterin diphosphateBy similarity | 1 | |
Binding sitei | 637 | 6-hydroxymethyl-7,8-dihydropterin diphosphateBy similarity | 1 | |
Binding sitei | 677 | 6-hydroxymethyl-7,8-dihydropterin diphosphateBy similarity | 1 |
GO - Molecular functioni
- 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase activity Source: PomBase
- ATP binding Source: UniProtKB-KW
- dihydroneopterin aldolase activity Source: PomBase
- dihydropteroate synthase activity Source: PomBase
- kinase activity Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- folic acid biosynthetic process Source: PomBase
- tetrahydrofolate biosynthetic process Source: PomBase
Keywordsi
Molecular function | Kinase, Lyase, Multifunctional enzyme, Transferase |
Biological process | Folate biosynthesis |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
UniPathwayi | UPA00077;UER00154 UPA00077;UER00155 UPA00077;UER00156 |
Names & Taxonomyi
Protein namesi | Recommended name: Folic acid synthesis protein fol1Including the following 3 domains: Alternative name(s): 7,8-dihydroneopterin aldolase FASA FASB Alternative name(s): 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase Short name: PPPK FASC Alternative name(s): Dihydropteroate pyrophosphorylase FASD |
Gene namesi | Name:fol1 ORF Names:SPBC1734.03, SPBC337.19 |
Organismi | Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) |
Taxonomic identifieri | 284812 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › |
Proteomesi |
|
Organism-specific databases
PomBasei | SPBC1734.03, fol1 |
VEuPathDBi | FungiDB:SPBC1734.03 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Cytosol
- cytosol Source: PomBase
Mitochondrion
- mitochondrial envelope Source: GO_Central
Other locations
- cytoplasm Source: PomBase
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000343164 | 1 – 733 | Folic acid synthesis protein fol1Add BLAST | 733 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 281 | Phosphotyrosine1 Publication | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | Q4LB35 |
PaxDbi | Q4LB35 |
PRIDEi | Q4LB35 |
PTM databases
iPTMneti | Q4LB35 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 276399, 3 interactors |
STRINGi | 4896.SPBC1734.03.1 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 465 – 724 | Pterin-bindingPROSITE-ProRule annotationAdd BLAST | 260 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 55 – 167 | DHNA 1Add BLAST | 113 | |
Regioni | 179 – 277 | DHNA 2Add BLAST | 99 | |
Regioni | 295 – 454 | HPKAdd BLAST | 160 | |
Regioni | 467 – 733 | DHPSAdd BLAST | 267 | |
Regioni | 712 – 714 | 6-hydroxymethyl-7,8-dihydropterin diphosphate bindingBy similarity | 3 |
Sequence similaritiesi
In the N-terminal section; belongs to the DHNA family.Curated
In the central section; belongs to the HPPK family.Curated
In the C-terminal section; belongs to the DHPS family.Curated
Phylogenomic databases
eggNOGi | KOG2544, Eukaryota |
HOGENOMi | CLU_008023_2_0_1 |
InParanoidi | Q4LB35 |
OMAi | LFESEPM |
Family and domain databases
CDDi | cd00534, DHNA_DHNTPE, 2 hits cd00739, DHPS, 1 hit cd00483, HPPK, 1 hit |
Gene3Di | 3.20.20.20, 1 hit 3.30.1130.10, 2 hits 3.30.70.560, 1 hit |
InterProi | View protein in InterPro IPR045031, DHP_synth IPR006390, DHP_synth_dom IPR011005, Dihydropteroate_synth-like IPR006157, FolB_dom IPR016261, Folic_acid_synth IPR043133, GTP-CH-I_C/QueF IPR000550, Hppk IPR035907, Hppk_sf IPR000489, Pterin-binding_dom |
PANTHERi | PTHR20941, PTHR20941, 1 hit |
Pfami | View protein in Pfam PF02152, FolB, 2 hits PF01288, HPPK, 1 hit PF00809, Pterin_bind, 1 hit |
PIRSFi | PIRSF000741, Folic_acid_synth, 1 hit |
SMARTi | View protein in SMART SM00905, FolB, 2 hits |
SUPFAMi | SSF51717, SSF51717, 1 hit SSF55083, SSF55083, 1 hit |
TIGRFAMsi | TIGR01496, DHPS, 1 hit TIGR01498, folK, 1 hit |
PROSITEi | View protein in PROSITE PS00793, DHPS_2, 1 hit PS50972, PTERIN_BINDING, 1 hit |
i Sequence
Sequence statusi: Complete.
Q4LB35-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKSLVNLWGI YPFIRNNSLH GFAKIPRIVS TIPRQLRFSS LRHPTRQMDL
60 70 80 90 100
IHDTVVVENL NTFAVVGQDQ WKRKEPQPVQ IDVYMRNNVQ LAGEKDELKS
110 120 130 140 150
TIHYGIASKL LRKEIEGSFF TTPKDLVNKI ASLCFEDVID TSHVSIKLTL
160 170 180 190 200
PKCVLRSKNG LHYYAERERN STSNFVDRIE FSDLELATIL GIHAFERQEK
210 220 230 240 250
QRVCLNISFA NTEVEALEIA RAIAEYVEQS AFLTIEALVV NLSKYLCFTK
260 270 280 290 300
NLDDISIKAE KPSAITFANA SAVQIYRTRS YFLQESLHKY ESTKNKIAYL
310 320 330 340 350
SFGSNIGDKF EQIQTALSML HKIEGIRVLD VSPLYETEPM YYKDQPSFLN
360 370 380 390 400
GVCKIETRMS PINLLRACQS IEQEMGRIKT ILKGPRCIDL DIVLYEDCVY
410 420 430 440 450
ESEVLTIPHL GLQEREFVLR PLLALSPDLV HPYTHQPLQE ALDKLPSQGI
460 470 480 490 500
RLYSSFDNKK IINGALTMGI LNVTPDSFSD GGKVSQNNIL EKAKSMVGDG
510 520 530 540 550
ASILDIGGQS TKPGADPVSV EEELRRVIPM ISLLRSSGIT VPISIDTYYS
560 570 580 590 600
KVAKLAIEAG ANIINDVTGG MGDEKMLPLA ASLQVPICIM HMRGTPETMK
610 620 630 640 650
ALSIYEKDIV EEVAVELSSR VEAAVQSGVH RYNIILDPGF GFAKTPKQSA
660 670 680 690 700
GLLGRLHELM KKPQFKDMHW LSGPSRKGFT GYFTGDASPK DRIWGTSACV
710 720 730
TASVLQGVSI VRVHDTKEMS KVVGMANAIR YVP
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CU329671 Genomic DNA Translation: CAA21289.2 |
PIRi | T39650 |
RefSeqi | NP_595420.2, NM_001021327.2 |
Genome annotation databases
EnsemblFungii | SPBC1734.03.1; SPBC1734.03.1:pep; SPBC1734.03 |
GeneIDi | 2539851 |
KEGGi | spo:SPBC1734.03 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CU329671 Genomic DNA Translation: CAA21289.2 |
PIRi | T39650 |
RefSeqi | NP_595420.2, NM_001021327.2 |
3D structure databases
AlphaFoldDBi | Q4LB35 |
SMRi | Q4LB35 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 276399, 3 interactors |
STRINGi | 4896.SPBC1734.03.1 |
PTM databases
iPTMneti | Q4LB35 |
Proteomic databases
MaxQBi | Q4LB35 |
PaxDbi | Q4LB35 |
PRIDEi | Q4LB35 |
Genome annotation databases
EnsemblFungii | SPBC1734.03.1; SPBC1734.03.1:pep; SPBC1734.03 |
GeneIDi | 2539851 |
KEGGi | spo:SPBC1734.03 |
Organism-specific databases
PomBasei | SPBC1734.03, fol1 |
VEuPathDBi | FungiDB:SPBC1734.03 |
Phylogenomic databases
eggNOGi | KOG2544, Eukaryota |
HOGENOMi | CLU_008023_2_0_1 |
InParanoidi | Q4LB35 |
OMAi | LFESEPM |
Enzyme and pathway databases
UniPathwayi | UPA00077;UER00154 UPA00077;UER00155 UPA00077;UER00156 |
Miscellaneous databases
PROi | PR:Q4LB35 |
Family and domain databases
CDDi | cd00534, DHNA_DHNTPE, 2 hits cd00739, DHPS, 1 hit cd00483, HPPK, 1 hit |
Gene3Di | 3.20.20.20, 1 hit 3.30.1130.10, 2 hits 3.30.70.560, 1 hit |
InterProi | View protein in InterPro IPR045031, DHP_synth IPR006390, DHP_synth_dom IPR011005, Dihydropteroate_synth-like IPR006157, FolB_dom IPR016261, Folic_acid_synth IPR043133, GTP-CH-I_C/QueF IPR000550, Hppk IPR035907, Hppk_sf IPR000489, Pterin-binding_dom |
PANTHERi | PTHR20941, PTHR20941, 1 hit |
Pfami | View protein in Pfam PF02152, FolB, 2 hits PF01288, HPPK, 1 hit PF00809, Pterin_bind, 1 hit |
PIRSFi | PIRSF000741, Folic_acid_synth, 1 hit |
SMARTi | View protein in SMART SM00905, FolB, 2 hits |
SUPFAMi | SSF51717, SSF51717, 1 hit SSF55083, SSF55083, 1 hit |
TIGRFAMsi | TIGR01496, DHPS, 1 hit TIGR01498, folK, 1 hit |
PROSITEi | View protein in PROSITE PS00793, DHPS_2, 1 hit PS50972, PTERIN_BINDING, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | FOL1_SCHPO | |
Accessioni | Q4LB35Primary (citable) accession number: Q4LB35 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 2008 |
Last sequence update: | October 3, 2012 | |
Last modified: | May 25, 2022 | |
This is version 104 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Schizosaccharomyces pombe
Schizosaccharomyces pombe: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families