Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Alanine dehydrogenase

Gene

ald

Organism
Staphylococcus haemolyticus (strain JCSC1435)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the reversible reductive amination of pyruvate to L-alanine. May play a role in cell wall synthesis as L-alanine is an important constituent of the peptidoglycan layer (By similarity).By similarity

Catalytic activityi

L-alanine + H2O + NAD+ = pyruvate + NH3 + NADH.

Pathwayi: L-alanine degradation via dehydrogenase pathway

This protein is involved in step 1 of the subpathway that synthesizes NH(3) and pyruvate from L-alanine.
Proteins known to be involved in this subpathway in this organism are:
  1. Alanine dehydrogenase (ald)
This subpathway is part of the pathway L-alanine degradation via dehydrogenase pathway, which is itself part of Amino-acid degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes NH(3) and pyruvate from L-alanine, the pathway L-alanine degradation via dehydrogenase pathway and in Amino-acid degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei15SubstrateBy similarity1
Binding sitei74SubstrateBy similarity1
Active sitei95Proton donor/acceptorSequence analysis1
Binding sitei133NADBy similarity1
Binding sitei197NADBy similarity1
Binding sitei219NADBy similarity1
Active sitei269Proton donor/acceptorBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi177 – 178NADBy similarity2
Nucleotide bindingi238 – 239NADBy similarity2
Nucleotide bindingi266 – 269NADBy similarity4
Nucleotide bindingi298 – 301NADBy similarity4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
LigandNAD, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00527; UER00585

Names & Taxonomyi

Protein namesi
Recommended name:
Alanine dehydrogenase (EC:1.4.1.1)
Gene namesi
Name:ald
Ordered Locus Names:SH1216
OrganismiStaphylococcus haemolyticus (strain JCSC1435)
Taxonomic identifieri279808 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000000543 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002873241 – 373Alanine dehydrogenaseAdd BLAST373

Interactioni

Subunit structurei

Homohexamer. Trimer of dimer (By similarity).By similarity

Protein-protein interaction databases

STRINGi279808.SH1216

Structurei

3D structure databases

ProteinModelPortaliQ4L750
SMRiQ4L750
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AlaDH/PNT family.Curated

Phylogenomic databases

eggNOGiENOG4105DCF Bacteria
COG0686 LUCA
HOGENOMiHOG000022120
KOiK00259
OMAiCFETSKA
OrthoDBiPOG091H02R2

Family and domain databases

CDDicd05305 L-AlaDH, 1 hit
InterProiView protein in InterPro
IPR008141 Ala_DH
IPR008143 Ala_DH/PNT_CS2
IPR008142 AlaDH/PNT_CS1
IPR007886 AlaDH/PNT_N
IPR007698 AlaDH/PNT_NAD(H)-bd
IPR036291 NAD(P)-bd_dom_sf
PANTHERiPTHR42795 PTHR42795, 1 hit
PfamiView protein in Pfam
PF01262 AlaDh_PNT_C, 1 hit
PF05222 AlaDh_PNT_N, 1 hit
PIRSFiPIRSF000183 Alanine_dh, 1 hit
SMARTiView protein in SMART
SM01002 AlaDh_PNT_C, 1 hit
SM01003 AlaDh_PNT_N, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
TIGRFAMsiTIGR00518 alaDH, 1 hit
PROSITEiView protein in PROSITE
PS00836 ALADH_PNT_1, 1 hit
PS00837 ALADH_PNT_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q4L750-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKIGIPKEIK NNENRVGLSP SGVHALVEQG HTVLVEKDAG LGSFFEDKDY
60 70 80 90 100
KDAGADIVSE QSSVWDVEMV IKVKEPLEEE YKYFKEGLIL FTYLHLANEE
110 120 130 140 150
KLTQALVDNK VVGIAYETVQ LPDRSLPLLT PMSEVAGRMS AQVGSQFLQK
160 170 180 190 200
FNGGMGILLG GVPGVPKGKV SIIGGGQAGT NAAKIALGLG ANVTILDVNP
210 220 230 240 250
KRLAELDDLF DGRVNTIMSN PLNIENAVKE SDLVIGAVLI PGAKAPSLVT
260 270 280 290 300
EDMIKQMKDG SVIVDIAIDQ GGIFETTDKI TTHDDPTYVK HGVVHYAVAN
310 320 330 340 350
MPGAVPRTST IALNNATLPY AQLLASKGYR EAFKANHALS LGLNTYKGHV
360 370
THKGVAEAFG LEYTSVEDAL KED
Length:373
Mass (Da):39,966
Last modified:August 2, 2005 - v1
Checksum:i66E91EE689108294
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP006716 Genomic DNA Translation: BAE04525.1
RefSeqiWP_011275515.1, NC_007168.1

Genome annotation databases

EnsemblBacteriaiBAE04525; BAE04525; SH1216
GeneIDi24248377
KEGGisha:SH1216

Similar proteinsi

Entry informationi

Entry nameiDHA_STAHJ
AccessioniPrimary (citable) accession number: Q4L750
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: August 2, 2005
Last modified: April 25, 2018
This is version 99 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome