UniProtKB - Q4KM49 (SYYC_RAT)
Protein
Tyrosine--tRNA ligase, cytoplasmic
Gene
Yars1
Organism
Rattus norvegicus (Rat)
Status
Functioni
Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr).By similarity
Catalytic activityi
- EC:6.1.1.1
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 39 | TyrosineBy similarity | 1 | |
Binding sitei | 166 | TyrosineBy similarity | 1 | |
Binding sitei | 170 | TyrosineBy similarity | 1 | |
Binding sitei | 173 | TyrosineBy similarity | 1 | |
Binding sitei | 188 | TyrosineBy similarity | 1 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- tRNA binding Source: UniProtKB-KW
- tyrosine-tRNA ligase activity Source: GO_Central
GO - Biological processi
- tyrosyl-tRNA aminoacylation Source: InterPro
Keywordsi
Molecular function | Aminoacyl-tRNA synthetase, Ligase, RNA-binding, tRNA-binding |
Biological process | Protein biosynthesis |
Ligand | ATP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Tyrosine--tRNA ligase, cytoplasmic (EC:6.1.1.1)Alternative name(s): Tyrosyl-tRNA synthetase Short name: TyrRS Cleaved into the following chain: |
Gene namesi | Name:Yars1 Synonyms:Yars |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 1307616, Yars |
Subcellular locationi
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000423288 | 1 – 528 | Tyrosine--tRNA ligase, cytoplasmicAdd BLAST | 528 | |
Initiator methioninei | Removed; alternateBy similarity | |||
ChainiPRO_0000239691 | 2 – 528 | Tyrosine--tRNA ligase, cytoplasmic, N-terminally processedAdd BLAST | 527 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineBy similarity | 1 | |
Modified residuei | 2 | N-acetylglycine; in Tyrosine--tRNA ligase, cytoplasmic, N-terminally processedBy similarity | 1 | |
Modified residuei | 197 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 205 | PhosphoserineBy similarity | 1 | |
Modified residuei | 206 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 386 | PhosphoserineBy similarity | 1 | |
Modified residuei | 474 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 482 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 490 | N6-acetyllysineBy similarity | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
jPOSTi | Q4KM49 |
PaxDbi | Q4KM49 |
PeptideAtlasi | Q4KM49 |
PRIDEi | Q4KM49 |
PTM databases
iPTMneti | Q4KM49 |
PhosphoSitePlusi | Q4KM49 |
Interactioni
Subunit structurei
Homodimer.
By similarityProtein-protein interaction databases
BioGRIDi | 260303, 1 interactor |
STRINGi | 10116.ENSRNOP00000010674 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 364 – 468 | tRNA-bindingPROSITE-ProRule annotationAdd BLAST | 105 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 44 – 52 | 'HIGH' regionBy similarity | 9 | |
Motifi | 222 – 226 | 'KMSKS' regionBy similarity | 5 |
Sequence similaritiesi
Belongs to the class-I aminoacyl-tRNA synthetase family.Curated
Phylogenomic databases
eggNOGi | KOG2144, Eukaryota KOG2241, Eukaryota |
HOGENOMi | CLU_035267_3_0_1 |
InParanoidi | Q4KM49 |
OrthoDBi | 852081at2759 |
PhylomeDBi | Q4KM49 |
Family and domain databases
CDDi | cd00805, TyrRS_core, 1 hit |
Gene3Di | 3.40.50.620, 1 hit |
InterProi | View protein in InterPro IPR002305, aa-tRNA-synth_Ic IPR012340, NA-bd_OB-fold IPR014729, Rossmann-like_a/b/a_fold IPR002547, tRNA-bd_dom IPR002307, Tyr-tRNA-ligase |
Pfami | View protein in Pfam PF00579, tRNA-synt_1b, 1 hit PF01588, tRNA_bind, 1 hit |
PRINTSi | PR01040, TRNASYNTHTYR |
SUPFAMi | SSF50249, SSF50249, 1 hit |
TIGRFAMsi | TIGR00234, tyrS, 1 hit |
PROSITEi | View protein in PROSITE PS50886, TRBD, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q4KM49-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGDAPSPEEK LHLITRNLQE VLGEEKLKEI LKERELKVYW GTATTGKPHV
60 70 80 90 100
AYFVPMSKIA DFLKAGCEVT ILFADLHAYL DNMKAPWELL ELRTSYYENV
110 120 130 140 150
IKAMLESIGV PLEKLKFTKG TDYQLSKEYT LDVYRLSSLV TQHDAKKAGA
160 170 180 190 200
EVVKQVEHPL LSGLLYPGLQ ALDEEYLKVD AQFGGIDQRK IFTFAEKYLP
210 220 230 240 250
TLGYSKRVHL MNPMVPGLTG SKMSSSEEES KIDLLDRKED VKKKLKKAFC
260 270 280 290 300
EPGNVENNGV LSFVKHVLFP LKSEFVILRD EKWGGNKTYT IYQELEKDFA
310 320 330 340 350
AEVVHPGDLK NSVEVALNKL LDPIREKFNT PALKKLASAA YPDPSKQKPT
360 370 380 390 400
AKGPAKSSEP EEIIPSRLDI RVGKILSVEK HPDADSLYVE KIDVGEAEPR
410 420 430 440 450
TVVSGLVQFV PKEELQDRLV VVLCNLKPQK MRGVDSQGML LCASVEGVSR
460 470 480 490 500
QVEPLDPPAG SAPGERVFVQ GYEKGQPDEE LKPKKKVFEK LQADFKISDD
510 520
CVAQWKQTNF MTKLGFVSCK SLKGGNIS
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A0H2UHG0 | A0A0H2UHG0_RAT | Tyrosine--tRNA ligase | Yars Yars1, rCG_31483 | 564 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC098795 mRNA Translation: AAH98795.1 |
RefSeqi | NP_001020867.2, NM_001025696.1 |
Genome annotation databases
GeneIDi | 313047 |
KEGGi | rno:313047 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC098795 mRNA Translation: AAH98795.1 |
RefSeqi | NP_001020867.2, NM_001025696.1 |
3D structure databases
SMRi | Q4KM49 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 260303, 1 interactor |
STRINGi | 10116.ENSRNOP00000010674 |
PTM databases
iPTMneti | Q4KM49 |
PhosphoSitePlusi | Q4KM49 |
Proteomic databases
jPOSTi | Q4KM49 |
PaxDbi | Q4KM49 |
PeptideAtlasi | Q4KM49 |
PRIDEi | Q4KM49 |
Genome annotation databases
GeneIDi | 313047 |
KEGGi | rno:313047 |
Organism-specific databases
CTDi | 8565 |
RGDi | 1307616, Yars |
Phylogenomic databases
eggNOGi | KOG2144, Eukaryota KOG2241, Eukaryota |
HOGENOMi | CLU_035267_3_0_1 |
InParanoidi | Q4KM49 |
OrthoDBi | 852081at2759 |
PhylomeDBi | Q4KM49 |
Miscellaneous databases
PROi | PR:Q4KM49 |
Gene expression databases
Genevisiblei | Q4KM49, RN |
Family and domain databases
CDDi | cd00805, TyrRS_core, 1 hit |
Gene3Di | 3.40.50.620, 1 hit |
InterProi | View protein in InterPro IPR002305, aa-tRNA-synth_Ic IPR012340, NA-bd_OB-fold IPR014729, Rossmann-like_a/b/a_fold IPR002547, tRNA-bd_dom IPR002307, Tyr-tRNA-ligase |
Pfami | View protein in Pfam PF00579, tRNA-synt_1b, 1 hit PF01588, tRNA_bind, 1 hit |
PRINTSi | PR01040, TRNASYNTHTYR |
SUPFAMi | SSF50249, SSF50249, 1 hit |
TIGRFAMsi | TIGR00234, tyrS, 1 hit |
PROSITEi | View protein in PROSITE PS50886, TRBD, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SYYC_RAT | |
Accessioni | Q4KM49Primary (citable) accession number: Q4KM49 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 13, 2006 |
Last sequence update: | January 23, 2007 | |
Last modified: | December 2, 2020 | |
This is version 115 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - Aminoacyl-tRNA synthetases
List of aminoacyl-tRNA synthetase entries