UniProtKB - Q4K5U4 (SYI_PSEF5)
Protein
Isoleucine--tRNA ligase
Gene
ileS
Organism
Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5)
Status
Functioni
Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation
Catalytic activityi
- EC:6.1.1.5UniRule annotation
Cofactori
Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 567 | Aminoacyl-adenylateUniRule annotation | 1 | |
Binding sitei | 611 | ATPUniRule annotation | 1 | |
Metal bindingi | 906 | ZincUniRule annotation | 1 | |
Metal bindingi | 909 | ZincUniRule annotation | 1 | |
Metal bindingi | 926 | ZincUniRule annotation | 1 | |
Metal bindingi | 929 | ZincUniRule annotation | 1 |
GO - Molecular functioni
- aminoacyl-tRNA editing activity Source: InterPro
- ATP binding Source: UniProtKB-UniRule
- isoleucine-tRNA ligase activity Source: UniProtKB-UniRule
- tRNA binding Source: InterPro
- zinc ion binding Source: UniProtKB-UniRule
GO - Biological processi
- isoleucyl-tRNA aminoacylation Source: UniProtKB-UniRule
Keywordsi
Molecular function | Aminoacyl-tRNA synthetase, Ligase |
Biological process | Protein biosynthesis |
Ligand | ATP-binding, Metal-binding, Nucleotide-binding, Zinc |
Enzyme and pathway databases
BioCyci | PPRO220664:G1G4K-5489-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)Alternative name(s): Isoleucyl-tRNA synthetaseUniRule annotation Short name: IleRSUniRule annotation |
Gene namesi | Name:ileSUniRule annotation Ordered Locus Names:PFL_5321 |
Organismi | Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5) |
Taxonomic identifieri | 220664 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › |
Proteomesi |
|
Subcellular locationi
- Cytoplasm UniRule annotation
GO - Cellular componenti
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000098446 | 1 – 943 | Isoleucine--tRNA ligaseAdd BLAST | 943 |
Proteomic databases
PRIDEi | Q4K5U4 |
Interactioni
Subunit structurei
Monomer.
UniRule annotationProtein-protein interaction databases
STRINGi | 220664.PFL_5321 |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 58 – 68 | 'HIGH' regionAdd BLAST | 11 | |
Motifi | 608 – 612 | 'KMSKS' region | 5 |
Domaini
IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0060, Bacteria |
HOGENOMi | CLU_001493_7_1_6 |
OMAi | HLGTAWN |
OrthoDBi | 32262at2 |
Family and domain databases
CDDi | cd07960, Anticodon_Ia_Ile_BEm, 1 hit |
Gene3Di | 3.40.50.620, 1 hit 3.90.740.10, 1 hit |
HAMAPi | MF_02002, Ile_tRNA_synth_type1, 1 hit |
InterProi | View protein in InterPro IPR001412, aa-tRNA-synth_I_CS IPR002300, aa-tRNA-synth_Ia IPR033708, Anticodon_Ile_BEm IPR002301, Ile-tRNA-ligase IPR023585, Ile-tRNA-ligase_type1 IPR013155, M/V/L/I-tRNA-synth_anticd-bd IPR014729, Rossmann-like_a/b/a_fold IPR009080, tRNAsynth_Ia_anticodon-bd IPR009008, Val/Leu/Ile-tRNA-synth_edit IPR010663, Znf_FPG/IleRS |
Pfami | View protein in Pfam PF08264, Anticodon_1, 1 hit PF00133, tRNA-synt_1, 1 hit PF06827, zf-FPG_IleRS, 1 hit |
PRINTSi | PR00984, TRNASYNTHILE |
SUPFAMi | SSF47323, SSF47323, 1 hit SSF50677, SSF50677, 1 hit |
TIGRFAMsi | TIGR00392, ileS, 1 hit |
PROSITEi | View protein in PROSITE PS00178, AA_TRNA_LIGASE_I, 1 hit |
i Sequence
Sequence statusi: Complete.
Q4K5U4-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTDYKATLNL PDTAFPMKAG LPQREPQILQ RWDSIGLYGK LREIGKDRPK
60 70 80 90 100
FVLHDGPPYA NGSIHIGHAV NKILKDMIIR SKTLAGFDAP YVPGWDCHGL
110 120 130 140 150
PIEHKVEVTH GKNLSSDQTR ELCRAYASEQ IEGQKAEFIR LGVLGDWANP
160 170 180 190 200
YKTMNFANEA GEIRALAEMV KGGFVFKGLK PVNWCFDCGS ALAEAEVEYQ
210 220 230 240 250
DKKSATIDVA FPVADEAKLA GAFGLAKLAK PAAIVIWTTT PWTIPANQAL
260 270 280 290 300
NVHPEFNYAL VDTGERLLVL AEELVESCLA RYNLEGSVIA TAPGSALELI
310 320 330 340 350
NFRHPFYDRL SPVYLAEYVE LGAGTGVVHS APAYGEDDFV TCKRYGMVND
360 370 380 390 400
DILNPVQSNG VYATSLEFFG GQFIWKANPA IVDKLSEVGA LLHTETISHS
410 420 430 440 450
YMHCWRHKTP LIYRATAQWF VGMDKQPAAG DTLRQRALQA IEDTQFVPAW
460 470 480 490 500
GQARLHSMIA NRPDWCISRQ RNWGVPIPFF LNKESGDLHP RTVELMEEVA
510 520 530 540 550
QRVEQEGIEA WFKMDAAELL GDEAAQYDKI SDTLDVWFDS GTTHWHVLRG
560 570 580 590 600
SHPMGHESGP RADLYLEGSD QHRGWFHSSL LTGCAIDNHA PYRELLTHGF
610 620 630 640 650
TVDENGRKMS KSLGNVIAPQ KVNDTLGADI MRLWVSATDY SGEMAVSDQI
660 670 680 690 700
LQRSADAYRR IRNTARFLLS NLSGFNPATD LLPAEDMLAL DRWAVDRTLL
710 720 730 740 750
LQRELELHYG EYRFWNVYSK VHNFCVQELG GFYLDIIKDR QYTTGANSKA
760 770 780 790 800
RRSAQTALYH ISEALVRWIA PILAFTADEL WEYLPGERNE SVMLNTWYEG
810 820 830 840 850
LTELPADFEL GREYWEGVMA VKVAVNKELE VQRAAKAVGG NLQAEVTLFA
860 870 880 890 900
EDGLAADLAK LSNELRFVLI TSTASLAPFA QAPADAVATE VPGLKLKVVK
910 920 930 940
SAFPKCARCW HCREDVGVHP EHPEICGRCV DNISGAGEVR HYA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP000076 Genomic DNA Translation: AAY94531.1 |
RefSeqi | WP_011063549.1, NC_004129.6 |
Genome annotation databases
EnsemblBacteriai | AAY94531; AAY94531; PFL_5321 |
KEGGi | pfl:PFL_5321 |
PATRICi | fig|220664.5.peg.5431 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP000076 Genomic DNA Translation: AAY94531.1 |
RefSeqi | WP_011063549.1, NC_004129.6 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
STRINGi | 220664.PFL_5321 |
Proteomic databases
PRIDEi | Q4K5U4 |
Genome annotation databases
EnsemblBacteriai | AAY94531; AAY94531; PFL_5321 |
KEGGi | pfl:PFL_5321 |
PATRICi | fig|220664.5.peg.5431 |
Phylogenomic databases
eggNOGi | COG0060, Bacteria |
HOGENOMi | CLU_001493_7_1_6 |
OMAi | HLGTAWN |
OrthoDBi | 32262at2 |
Enzyme and pathway databases
BioCyci | PPRO220664:G1G4K-5489-MONOMER |
Family and domain databases
CDDi | cd07960, Anticodon_Ia_Ile_BEm, 1 hit |
Gene3Di | 3.40.50.620, 1 hit 3.90.740.10, 1 hit |
HAMAPi | MF_02002, Ile_tRNA_synth_type1, 1 hit |
InterProi | View protein in InterPro IPR001412, aa-tRNA-synth_I_CS IPR002300, aa-tRNA-synth_Ia IPR033708, Anticodon_Ile_BEm IPR002301, Ile-tRNA-ligase IPR023585, Ile-tRNA-ligase_type1 IPR013155, M/V/L/I-tRNA-synth_anticd-bd IPR014729, Rossmann-like_a/b/a_fold IPR009080, tRNAsynth_Ia_anticodon-bd IPR009008, Val/Leu/Ile-tRNA-synth_edit IPR010663, Znf_FPG/IleRS |
Pfami | View protein in Pfam PF08264, Anticodon_1, 1 hit PF00133, tRNA-synt_1, 1 hit PF06827, zf-FPG_IleRS, 1 hit |
PRINTSi | PR00984, TRNASYNTHILE |
SUPFAMi | SSF47323, SSF47323, 1 hit SSF50677, SSF50677, 1 hit |
TIGRFAMsi | TIGR00392, ileS, 1 hit |
PROSITEi | View protein in PROSITE PS00178, AA_TRNA_LIGASE_I, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SYI_PSEF5 | |
Accessioni | Q4K5U4Primary (citable) accession number: Q4K5U4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 20, 2005 |
Last sequence update: | August 2, 2005 | |
Last modified: | February 10, 2021 | |
This is version 104 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Aminoacyl-tRNA synthetases
List of aminoacyl-tRNA synthetase entries - SIMILARITY comments
Index of protein domains and families