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Protein

Thiamine thiazole synthase

Gene

thi4

Organism
Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the biosynthesis of the thiazole moiety of thiamine. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylate (ADT), an adenylated thiazole intermediate, using free sulfide as a source of sulfur.UniRule annotation

Cofactori

Fe2+UniRule annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei43NAD; via amide nitrogenUniRule annotation1
Binding sitei70NAD; via amide nitrogen and carbonyl oxygenUniRule annotation1
Binding sitei134NAD; via amide nitrogen and carbonyl oxygenUniRule annotation1
Metal bindingi164Iron; shared with adjacent protomerUniRule annotation1
Metal bindingi179IronUniRule annotation1
Binding sitei229NAD; via amide nitrogenUniRule annotation1
Binding sitei239GlycineUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi62 – 63NADUniRule annotation2
Nucleotide bindingi162 – 164NAD; shared with adjacent protomerUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processThiamine biosynthesis
LigandIron, Metal-binding, NAD

Enzyme and pathway databases

UniPathwayi
UPA00060

Names & Taxonomyi

Protein namesi
Recommended name:
Thiamine thiazole synthaseUniRule annotation (EC:4.-.-.-UniRule annotation)
Gene namesi
Name:thi4UniRule annotation
Ordered Locus Names:Saci_0854
OrganismiSulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)
Taxonomic identifieri330779 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
Proteomesi
  • UP000001018 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003007391 – 265Thiamine thiazole synthaseAdd BLAST265

Interactioni

Subunit structurei

Homooctamer; tetramer of dimers.UniRule annotation

Protein-protein interaction databases

STRINGi330779.Saci_0854

Structurei

3D structure databases

ProteinModelPortaliQ4JAF8
SMRiQ4JAF8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the THI4 family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00574 Archaea
COG1635 LUCA
HOGENOMiHOG000106048
KOiK22699
OMAiMWGGGMM
OrthoDBiPOG093Z09PU

Family and domain databases

Gene3Di3.50.50.60, 1 hit
HAMAPiMF_00304 Thi4, 1 hit
InterProiView protein in InterPro
IPR036188 FAD/NAD-bd_sf
IPR002922 Thi4_fam
IPR022828 Thi4_prok
SUPFAMiSSF51905 SSF51905, 1 hit
TIGRFAMsiTIGR00292 TIGR00292, 1 hit

Sequencei

Sequence statusi: Complete.

Q4JAF8-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSDSIKIKAI DEVKISRYII KQTMEDWMNF VENDVVIVGA GPAGMSAAYY
60 70 80 90 100
LAKHGLKTLV FERRLSFGGG IGGGAMLFHK LVIESPADEV LKEMNIRLEK
110 120 130 140 150
VEDGVYIVDS AEFMAKLAAS AIDAGAKIIH GVTVDDVIFR ENPLRVAGVA
160 170 180 190 200
VEWTATQMAG LHVDPVFISA KAVVDATGHD AEVVAVASRK IPELGIVIPG
210 220 230 240 250
ERSAYSEMAE KLTVEQTGVV APGLYVAGMS VTEVRGLPRM GPIFGSMVLS
260
GKKVAEDIIK DLRNS
Length:265
Mass (Da):28,388
Last modified:August 2, 2005 - v1
Checksum:iC1D84D14A0148EB4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000077 Genomic DNA Translation: AAY80221.1
RefSeqiWP_011277723.1, NC_007181.1

Genome annotation databases

EnsemblBacteriaiAAY80221; AAY80221; Saci_0854
GeneIDi3472725
KEGGisai:Saci_0854
PATRICifig|330779.12.peg.818

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000077 Genomic DNA Translation: AAY80221.1
RefSeqiWP_011277723.1, NC_007181.1

3D structure databases

ProteinModelPortaliQ4JAF8
SMRiQ4JAF8
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi330779.Saci_0854

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAY80221; AAY80221; Saci_0854
GeneIDi3472725
KEGGisai:Saci_0854
PATRICifig|330779.12.peg.818

Phylogenomic databases

eggNOGiarCOG00574 Archaea
COG1635 LUCA
HOGENOMiHOG000106048
KOiK22699
OMAiMWGGGMM
OrthoDBiPOG093Z09PU

Enzyme and pathway databases

UniPathwayi
UPA00060

Family and domain databases

Gene3Di3.50.50.60, 1 hit
HAMAPiMF_00304 Thi4, 1 hit
InterProiView protein in InterPro
IPR036188 FAD/NAD-bd_sf
IPR002922 Thi4_fam
IPR022828 Thi4_prok
SUPFAMiSSF51905 SSF51905, 1 hit
TIGRFAMsiTIGR00292 TIGR00292, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTHI4_SULAC
AccessioniPrimary (citable) accession number: Q4JAF8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: August 2, 2005
Last modified: October 10, 2018
This is version 82 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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Main funding by: National Institutes of Health

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