UniProtKB - Q4J8E7 (ARGX_SULAC)
Protein
Glutamate--LysW ligase ArgX
Gene
argX
Organism
Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)
Status
Functioni
Catalyzes the ATP-dependent formation of a covalent bond between the amino group of glutamate and the gamma-carboxyl group of the C-terminal glutamate residue in LysW.1 Publication
Catalytic activityi
Cofactori
Mg2+By similarityNote: Binds 2 magnesium ions per subunit.By similarity
: L-arginine biosynthesis Pathwayi
This protein is involved in the pathway L-arginine biosynthesis, which is part of Amino-acid biosynthesis.1 PublicationView all proteins of this organism that are known to be involved in the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 87 | ATPBy similarity | 1 | |
Binding sitei | 127 | ATPBy similarity | 1 | |
Binding sitei | 192 | SubstrateBy similarity | 1 | |
Binding sitei | 202 | ATPBy similarity | 1 | |
Metal bindingi | 237 | Magnesium 1By similarity | 1 | |
Metal bindingi | 250 | Magnesium 1By similarity | 1 | |
Metal bindingi | 250 | Magnesium 2By similarity | 1 | |
Metal bindingi | 252 | Magnesium 2By similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 131 – 137 | ATPPROSITE-ProRule annotation | 7 | |
Nucleotide bindingi | 167 – 178 | ATPPROSITE-ProRule annotationAdd BLAST | 12 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- ligase activity Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- arginine biosynthetic process Source: UniProtKB-UniPathway
- cellular protein modification process Source: InterPro
- lysine biosynthetic process Source: InterPro
Keywordsi
Molecular function | Ligase |
Biological process | Amino-acid biosynthesis, Arginine biosynthesis |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | MetaCyc:MONOMER-18311 SACI330779:G1G4L-1585-MONOMER |
BRENDAi | 6.3.2.B19 6160 |
UniPathwayi | UPA00068 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:argX1 Publication Ordered Locus Names:Saci_1621 |
Organismi | Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770) |
Taxonomic identifieri | 330779 [NCBI] |
Taxonomic lineagei | Archaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Sulfolobus › |
Proteomesi |
|
Pathology & Biotechi
Disruption phenotypei
Cells lacking this gene require arginine for growth.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000422991 | 1 – 282 | Glutamate--LysW ligase ArgXAdd BLAST | 282 |
Interactioni
Subunit structurei
Homotetramer.
Interacts with LysW.
By similarityProtein-protein interaction databases
STRINGi | 330779.Saci_1621 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 91 – 277 | ATP-graspPROSITE-ProRule annotationAdd BLAST | 187 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 203 – 204 | Substrate bindingBy similarity | 2 | |
Regioni | 256 – 260 | Substrate bindingBy similarity | 5 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 259 – 260 | GF motif that is essential for ArgX substrate specificity1 Publication | 2 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | arCOG01589 Archaea COG0189 LUCA |
HOGENOMi | HOG000228553 |
KOi | K19412 |
OMAi | VDKSMTS |
OrthoDBi | 42812at2157 |
Family and domain databases
Gene3Di | 3.30.1490.20, 1 hit |
InterProi | View protein in InterPro IPR011761 ATP-grasp IPR013651 ATP-grasp_RimK-type IPR013815 ATP_grasp_subdomain_1 IPR011870 LysX_arch IPR016185 PreATP-grasp_dom_sf IPR004666 RpS6_RimK/Lys_biosynth_LsyX |
Pfami | View protein in Pfam PF08443 RimK, 1 hit |
SUPFAMi | SSF52440 SSF52440, 1 hit |
TIGRFAMsi | TIGR02144 LysX_arch, 1 hit TIGR00768 rimK_fam, 1 hit |
PROSITEi | View protein in PROSITE PS50975 ATP_GRASP, 1 hit |
i Sequence
Sequence statusi: Complete.
Q4J8E7-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRVALVVDIV RQEEKLIAKA LEKFQLQYDV INVAQEPLPF NKALGRYDVA
60 70 80 90 100
IIRPISMYRA LYASAVLESA GVHTINSSDT ISLCGDKILT YSKLYREGIP
110 120 130 140 150
IPDSIIAMSS DAALKAYEQK GFPLIDKPPI GSWGRLVSLI RDIFEGKTII
160 170 180 190 200
EHRELMGNSA LKVHIVQEYI NYKSRDIRCI VIGSELLGCY ARNIPSNEWR
210 220 230 240 250
ANIALGGYPS QIEVDHKLKE TVLKATSIIG GEFVSIDVME HQSKNYVINE
260 270 280
FNDVPEFKGF MLATNIDVAE ELVSYVKNNY LR
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP000077 Genomic DNA Translation: AAY80933.1 |
RefSeqi | WP_011278435.1, NC_007181.1 |
Genome annotation databases
EnsemblBacteriai | AAY80933; AAY80933; Saci_1621 |
GeneIDi | 3474177 |
KEGGi | sai:Saci_1621 |
PATRICi | fig|330779.12.peg.1560 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP000077 Genomic DNA Translation: AAY80933.1 |
RefSeqi | WP_011278435.1, NC_007181.1 |
3D structure databases
SMRi | Q4J8E7 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 330779.Saci_1621 |
Genome annotation databases
EnsemblBacteriai | AAY80933; AAY80933; Saci_1621 |
GeneIDi | 3474177 |
KEGGi | sai:Saci_1621 |
PATRICi | fig|330779.12.peg.1560 |
Phylogenomic databases
eggNOGi | arCOG01589 Archaea COG0189 LUCA |
HOGENOMi | HOG000228553 |
KOi | K19412 |
OMAi | VDKSMTS |
OrthoDBi | 42812at2157 |
Enzyme and pathway databases
UniPathwayi | UPA00068 |
BioCyci | MetaCyc:MONOMER-18311 SACI330779:G1G4L-1585-MONOMER |
BRENDAi | 6.3.2.B19 6160 |
Family and domain databases
Gene3Di | 3.30.1490.20, 1 hit |
InterProi | View protein in InterPro IPR011761 ATP-grasp IPR013651 ATP-grasp_RimK-type IPR013815 ATP_grasp_subdomain_1 IPR011870 LysX_arch IPR016185 PreATP-grasp_dom_sf IPR004666 RpS6_RimK/Lys_biosynth_LsyX |
Pfami | View protein in Pfam PF08443 RimK, 1 hit |
SUPFAMi | SSF52440 SSF52440, 1 hit |
TIGRFAMsi | TIGR02144 LysX_arch, 1 hit TIGR00768 rimK_fam, 1 hit |
PROSITEi | View protein in PROSITE PS50975 ATP_GRASP, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ARGX_SULAC | |
Accessioni | Q4J8E7Primary (citable) accession number: Q4J8E7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 24, 2013 |
Last sequence update: | August 2, 2005 | |
Last modified: | September 18, 2019 | |
This is version 70 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Complete proteome, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - PATHWAY comments
Index of metabolic and biosynthesis pathways