UniProtKB - Q4H4E7 (BTRJ_BACCI)
Protein
[Butirosin acyl-carrier protein]--L-glutamate ligase
Gene
btrJ
Organism
Bacillus circulans
Status
Functioni
ATP-dependent ligase that catalyzes 2 steps in the biosynthesis of the side chain of the aminoglycoside antibiotics in the biosynthetic pathway of butirosin. Mediates the addition of one molecule of L-glutamate to a dedicated acyl-carrier protein. Following decarboxylation of the product by BtrK, adds a second L-glutamate molecule.1 Publication
Catalytic activityi
- EC:6.2.1.391 Publication
- 4-aminobutanoyl-[BtrI ACP] + ATP + L-glutamate = 4-(γ-L-glutamylamino)butanoy-[BtrI ACP] + ADP + H+ + phosphate1 PublicationEC:6.2.1.391 Publication
Cofactori
Mg2+1 Publication, Mn2+1 PublicationNote: Binds 2 magnesium or manganese ions per subunit.1 Publication
: butirosin biosynthesis Pathwayi
This protein is involved in the pathway butirosin biosynthesis, which is part of Antibiotic biosynthesis.1 PublicationView all proteins of this organism that are known to be involved in the pathway butirosin biosynthesis and in Antibiotic biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 298 | Magnesium or manganese 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 312 | Magnesium or manganese 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 312 | Magnesium or manganese 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 314 | Magnesium or manganese 2PROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 174 – 231 | ATPPROSITE-ProRule annotationAdd BLAST | 58 |
GO - Molecular functioni
- acid-thiol ligase activity Source: UniProtKB
- ATP binding Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- antibiotic biosynthetic process Source: UniProtKB
Keywordsi
Molecular function | Ligase |
Biological process | Antibiotic biosynthesis |
Ligand | ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | MetaCyc:MONOMER-17271 |
BRENDAi | 6.2.1.39, 649 |
UniPathwayi | UPA00964 |
Names & Taxonomyi
Protein namesi | Recommended name: [Butirosin acyl-carrier protein]--L-glutamate ligase (EC:6.2.1.39)Alternative name(s): Butirosin biosynthesis protein J |
Gene namesi | Name:btrJ |
Organismi | Bacillus circulans |
Taxonomic identifieri | 1397 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000421731 | 1 – 419 | [Butirosin acyl-carrier protein]--L-glutamate ligaseAdd BLAST | 419 |
Interactioni
Subunit structurei
Monomer.
1 PublicationFamily & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 144 – 345 | ATP-graspPROSITE-ProRule annotationAdd BLAST | 202 |
Family and domain databases
InterProi | View protein in InterPro IPR011761, ATP-grasp IPR003806, ATP-grasp_PylC-type |
Pfami | View protein in Pfam PF02655, ATP-grasp_3, 1 hit |
PROSITEi | View protein in PROSITE PS50975, ATP_GRASP, 1 hit |
i Sequence
Sequence statusi: Complete.
Q4H4E7-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKFTHPLDYY RLNGKQILWY MNIGEDQDSQ ASNYFPSVKD PQSEKIVVQQ
60 70 80 90 100
EQQLLFLARP QDTVFFHTMP EQAFLDYWKE RRLSLPSIIC CDKLSQVPDL
110 120 130 140 150
ERYTIIPFIV SDQLLELKRR YPHMDIIAPD LAVCREINHK FNTRRLMERN
160 170 180 190 200
GFNVTTGYFC SDIESLEHAY EQLISAGFSK CVLKVPYGSS GKGLKVIDNE
210 220 230 240 250
RNFRFLLNYI QNRQTNVDLL LEGWHPHRLS LTSQLFITEY EVHLLAVTEQ
260 270 280 290 300
IIDPNGVYKG TNFTPALSQS EAADYREEIL RAGELIRQMG YRGVLGIDSI
310 320 330 340 350
LDTNGELIPV IEINARLTQV TYILPLVIEQ KKRYEFVESR VLVFNSRADL
360 370 380 390 400
DFEDYENDLS EVTRDLPVRI DLYNFCKASG AFKNTYKLFV LVSAHNSEQL
410
IKARSLLDEL NTKMTTAVH
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB097196 Genomic DNA Translation: BAE07074.1 AJ781030 Genomic DNA Translation: CAG77428.2 |
Genome annotation databases
KEGGi | ag:BAE07074 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB097196 Genomic DNA Translation: BAE07074.1 AJ781030 Genomic DNA Translation: CAG77428.2 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Genome annotation databases
KEGGi | ag:BAE07074 |
Enzyme and pathway databases
UniPathwayi | UPA00964 |
BioCyci | MetaCyc:MONOMER-17271 |
BRENDAi | 6.2.1.39, 649 |
Family and domain databases
InterProi | View protein in InterPro IPR011761, ATP-grasp IPR003806, ATP-grasp_PylC-type |
Pfami | View protein in Pfam PF02655, ATP-grasp_3, 1 hit |
PROSITEi | View protein in PROSITE PS50975, ATP_GRASP, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | BTRJ_BACCI | |
Accessioni | Q4H4E7Primary (citable) accession number: Q4H4E7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 6, 2013 |
Last sequence update: | August 30, 2005 | |
Last modified: | December 2, 2020 | |
This is version 52 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |