UniProtKB - Q4GZT4 (ABCG2_BOVIN)
Broad substrate specificity ATP-binding cassette transporter ABCG2
ABCG2
Functioni
Broad substrate specificity ATP-dependent transporter of the ATP-binding cassette (ABC) family that actively extrudes a wide variety of physiological compounds, dietary toxins and xenobiotics from cells. Involved in porphyrin homeostasis, mediating the export of protoporphyrin IX (PPIX) from both mitochondria to cytosol and cytosol to extracellular space, it also functions in the cellular export of heme. Also mediates the efflux of sphingosine-1-P from cells. Acts as a urate exporter functioning in both renal and extrarenal urate excretion (By similarity).
In kidney, it also functions as a physiological exporter of the uremic toxin indoxyl sulfate (By similarity).
Also involved in the excretion of steroids like estrone 3-sulfate/E1S, 3beta-sulfooxy-androst-5-en-17-one/DHEAS, and other sulfate conjugates (By similarity).
Mediates the secretion of the riboflavin and biotin vitamins into milk. Extrudes pheophorbide a, a phototoxic porphyrin catabolite of chlorophyll, reducing its bioavailability (By similarity).
Plays an important role in the exclusion of xenobiotics from the brain. It confers to cells a resistance to multiple drugs and other xenobiotics including mitoxantrone, pheophorbide, camptothecin, methotrexate, azidothymidine, and the anthracyclines daunorubicin and doxorubicin, through the control of their efflux (By similarity).
In placenta, it limits the penetration of drugs from the maternal plasma into the fetus. May play a role in early stem cell self-renewal by blocking differentiation (By similarity).
By similarityCatalytic activityi
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- ATP + H2O + sphing-4-enine 1-phosphate(in) = ADP + H+ + phosphate + sphing-4-enine 1-phosphate(out)By similarityThis reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- 3β-sulfooxy-androst-5-en-17-one(in) + ATP + H2O = 3β-sulfooxy-androst-5-en-17-one(out) + ADP + H+ + phosphateBy similarityThis reaction proceeds in the forwardBy similarity direction.
- 4-methylumbelliferone sulfate(in) + ATP + H2O = 4-methylumbelliferone sulfate(out) + ADP + H+ + phosphateBy similarityThis reaction proceeds in the forwardBy similarity direction.
- 5,7-dimethyl-2-methylamino-4-(3-pyridylmethyl)-1,3-benzothiazol-6-yl β-D-glucuronate(in) + ATP + H2O = 5,7-dimethyl-2-methylamino-4-(3-pyridylmethyl)-1,3-benzothiazol-6-yl β-D-glucuronate(out) + ADP + H+ + phosphateBy similarityThis reaction proceeds in the forwardBy similarity direction.
- 4-methylumbelliferone β-D-glucuronate(in) + ATP + H2O = 4-methylumbelliferone β-D-glucuronate(out) + ADP + H+ + phosphateBy similarityThis reaction proceeds in the forwardBy similarity direction.
- 5,7-dimethyl-2-methylamino-4-(3-pyridylmethyl)-1,3-benzothiazol-6-yl sulfate(in) + ATP + H2O = 5,7-dimethyl-2-methylamino-4-(3-pyridylmethyl)-1,3-benzothiazol-6-yl sulfate(out) + ADP + H+ + phosphateBy similarityThis reaction proceeds in the forwardBy similarity direction.
- 17β-estradiol 17-O-(β-D-glucuronate)(in) + ATP + H2O = 17β-estradiol 17-O-(β-D-glucuronate)(out) + ADP + H+ + phosphateBy similarityThis reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- ATP + H(2)O + xenobiotic(Side 1) = ADP + phosphate + xenobiotic(Side 2).By similarity EC:7.6.2.2
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 210 | ATPBy similarity | 1 | |
Binding sitei | 242 | ATPBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 79 – 86 | ATPPROSITE-ProRule annotation | 8 | |
Nucleotide bindingi | 183 – 189 | ATPBy similarity | 7 |
GO - Molecular functioni
- ABC-type xenobiotic transporter activity Source: UniProtKB-EC
- ATPase-coupled transmembrane transporter activity Source: UniProtKB
- ATP binding Source: UniProtKB-KW
- biotin transmembrane transporter activity Source: UniProtKB
- efflux transmembrane transporter activity Source: UniProtKB
- riboflavin transmembrane transporter activity Source: UniProtKB
- urate transmembrane transporter activity Source: UniProtKB
GO - Biological processi
- biotin transport Source: UniProtKB
- lipid transport Source: UniProtKB-KW
- renal urate salt excretion Source: UniProtKB
- riboflavin transport Source: UniProtKB
- transmembrane transport Source: UniProtKB
Keywordsi
Molecular function | Translocase |
Biological process | Lipid transport, Transport |
Ligand | ATP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Broad substrate specificity ATP-binding cassette transporter ABCG2Curated (EC:7.6.2.2By similarity)Alternative name(s): ATP-binding cassette sub-family G member 2 Urate exporter CD_antigen: CD338 |
Gene namesi | Name:ABCG2 |
Organismi | Bos taurus (Bovine) |
Taxonomic identifieri | 9913 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos |
Proteomesi |
|
Subcellular locationi
Mitochondrion
- Mitochondrion membrane By similarity; Multi-pass membrane protein Sequence analysis
Plasma membrane
- Cell membrane By similarity; Multi-pass membrane protein Sequence analysis
- Apical cell membrane By similarity; Multi-pass membrane protein Sequence analysis
Note: Enriched in membrane lipid rafts.By similarity
Mitochondrion
- mitochondrial membrane Source: UniProtKB-SubCell
Plasma Membrane
- apical plasma membrane Source: UniProtKB
- brush border membrane Source: UniProtKB
- plasma membrane Source: GO_Central
Other locations
- integral component of membrane Source: UniProtKB-KW
- membrane raft Source: UniProtKB
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 395 | CytoplasmicSequence analysisAdd BLAST | 395 | |
Transmembranei | 396 – 416 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 417 – 428 | ExtracellularSequence analysisAdd BLAST | 12 | |
Transmembranei | 429 – 449 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 450 – 477 | CytoplasmicSequence analysisAdd BLAST | 28 | |
Transmembranei | 478 – 498 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 499 – 506 | ExtracellularSequence analysis | 8 | |
Transmembranei | 507 – 527 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 528 – 535 | CytoplasmicSequence analysis | 8 | |
Transmembranei | 536 – 556 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 557 – 630 | ExtracellularSequence analysisAdd BLAST | 74 | |
Transmembranei | 631 – 651 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 652 – 655 | CytoplasmicSequence analysis | 4 |
Keywords - Cellular componenti
Cell membrane, Membrane, MitochondrionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000244032 | 1 – 655 | Broad substrate specificity ATP-binding cassette transporter ABCG2Add BLAST | 655 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 592 ↔ 608 | By similarity | ||
Glycosylationi | 596 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 600 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 603 | InterchainBy similarity |
Post-translational modificationi
Keywords - PTMi
Disulfide bond, Glycoprotein, PhosphoproteinProteomic databases
PaxDbi | Q4GZT4 |
PRIDEi | Q4GZT4 |
Interactioni
Subunit structurei
Homodimer; disulfide-linked. The minimal functional unit is a homodimer, but the major oligomeric form in plasma membrane is a homotetramer with possibility of higher order oligomerization up to homododecamers.
By similarityProtein-protein interaction databases
STRINGi | 9913.ENSBTAP00000051068 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 36 – 285 | ABC transporterPROSITE-ProRule annotationAdd BLAST | 250 | |
Domaini | 389 – 651 | ABC transmembrane type-2Add BLAST | 263 |
Domaini
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0061, Eukaryota |
HOGENOMi | CLU_000604_57_8_1 |
InParanoidi | Q4GZT4 |
Family and domain databases
Gene3Di | 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR013525, ABC_2_trans IPR003439, ABC_transporter-like_ATP-bd IPR030256, ABCG2 IPR043926, ABCG_dom IPR027417, P-loop_NTPase |
PANTHERi | PTHR48041:SF92, PTHR48041:SF92, 1 hit |
Pfami | View protein in Pfam PF01061, ABC2_membrane, 1 hit PF19055, ABC2_membrane_7, 1 hit PF00005, ABC_tran, 1 hit |
SMARTi | View protein in SMART SM00382, AAA, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS50893, ABC_TRANSPORTER_2, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MSSNSYEVSI PMSKKLNGIP ETTSKDLQTL TEGAVLSFHN ICYRVKVKTG
60 70 80 90 100
FLLCRKTIEK EILANINGVM KPGLNAILGP TGGGKSSLLD ILAARKDPHG
110 120 130 140 150
LSGDVLINGA PRPANFKCNS GYVVQDDVVM GTLTVRENLQ FSAALRLPTT
160 170 180 190 200
MTSYEKNERI NKVIQELGLD KVADSKVGTQ FIRGVSGGER KRTSIAMELI
210 220 230 240 250
TDPSILFLDE PTTGLDSSTA NAVLLLLKRM SKQGRTIIFS IHQPRYSIFK
260 270 280 290 300
LFDSLTLLAS GRLMFHGPAQ EALGYFGAIG FRCEPYNNPA DFFLDIINGD
310 320 330 340 350
SSAVVLNRED IGDEANETEE PSKKDTPLIE KLAEFYVNSS FFKETKVELD
360 370 380 390 400
KFSGDQRRKK LPSYKEVTYA TSFCHQLKWI SRRSFKNLLG NPQASIAQLI
410 420 430 440 450
VTVFLGLVIG AIFYDLKNDP AGIQNRAGVL FFLTTNQCFS SVSAVELLVV
460 470 480 490 500
EKKLFIHEYI SGYYRVSSYF FGKLLSDLLP MRMLPSIIFT CITYFLLGLK
510 520 530 540 550
PKVEAFFIMM LTLMMVAYSA SSMALAIAAG QSVVSIATLL MTISFVFMMI
560 570 580 590 600
FSGLLVNLKT VVPWLSWLQY LSIPRYGYAA LQHNEFLGQN FCPGLNVTTN
610 620 630 640 650
NTCSYAICTG EEFLTNQGID ISPWGLWKNH VALACMIVIF LTIAYLKLLF
LKKFS
Sequence cautioni
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 578 | Y → S Affecting milk fat and protein concentration. 1 Publication | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ871176 Genomic DNA Translation: CAI38796.1 Different initiation. |
RefSeqi | XP_005207851.1, XM_005207794.2 XP_005207852.1, XM_005207795.3 XP_005207853.1, XM_005207796.2 XP_005207854.1, XM_005207797.3 XP_010804335.1, XM_010806033.2 XP_010804337.1, XM_010806035.2 |
Genome annotation databases
GeneIDi | 536203 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ871176 Genomic DNA Translation: CAI38796.1 Different initiation. |
RefSeqi | XP_005207851.1, XM_005207794.2 XP_005207852.1, XM_005207795.3 XP_005207853.1, XM_005207796.2 XP_005207854.1, XM_005207797.3 XP_010804335.1, XM_010806033.2 XP_010804337.1, XM_010806035.2 |
3D structure databases
AlphaFoldDBi | Q4GZT4 |
SMRi | Q4GZT4 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9913.ENSBTAP00000051068 |
Proteomic databases
PaxDbi | Q4GZT4 |
PRIDEi | Q4GZT4 |
Genome annotation databases
GeneIDi | 536203 |
Organism-specific databases
CTDi | 9429 |
Phylogenomic databases
eggNOGi | KOG0061, Eukaryota |
HOGENOMi | CLU_000604_57_8_1 |
InParanoidi | Q4GZT4 |
Family and domain databases
Gene3Di | 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR013525, ABC_2_trans IPR003439, ABC_transporter-like_ATP-bd IPR030256, ABCG2 IPR043926, ABCG_dom IPR027417, P-loop_NTPase |
PANTHERi | PTHR48041:SF92, PTHR48041:SF92, 1 hit |
Pfami | View protein in Pfam PF01061, ABC2_membrane, 1 hit PF19055, ABC2_membrane_7, 1 hit PF00005, ABC_tran, 1 hit |
SMARTi | View protein in SMART SM00382, AAA, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS50893, ABC_TRANSPORTER_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ABCG2_BOVIN | |
Accessioni | Q4GZT4Primary (citable) accession number: Q4GZT4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 27, 2006 |
Last sequence update: | June 27, 2006 | |
Last modified: | May 25, 2022 | |
This is version 109 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families