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Protein

Dual specificity phosphatase 28

Gene

DUSP28

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Has phosphatase activity with the synthetic substrate 6,8-difluoro-4-methylumbelliferyl phosphate (in vitro) (PubMed:24531476, PubMed:29121083). Has almost no detectable activity with phosphotyrosine, even less activity with phosphothreonine and displays complete lack of activity with phosphoserine (PubMed:29121083). The poor activity with phosphotyrosine may be due to steric hindrance by bulky amino acid sidechains that obstruct access to the active site (PubMed:29121083).2 Publications

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.
[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

pH dependencei

Optimum pH is 6.0.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei103Phosphocysteine intermediate1 Publication1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protein phosphatase

Names & Taxonomyi

Protein namesi
Recommended name:
Dual specificity phosphatase 28 (EC:3.1.3.16, EC:3.1.3.48)
Gene namesi
Name:DUSP28
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

EuPathDBiHostDB:ENSG00000188542.9
HGNCiHGNC:33237 DUSP28
neXtProtiNX_Q4G0W2

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi102Y → H: Decreases the already low catalytic activity. 1 Publication1
Mutagenesisi103C → S: Loss of catalytic activity. 1 Publication1
Mutagenesisi105N → A: Increases activity with phosphotyrosine; when associated with V-107. 1 Publication1
Mutagenesisi107R → V: Increases activity with phosphotyrosine; when associated with A-105. 1 Publication1

Organism-specific databases

DisGeNETi285193
OpenTargetsiENSG00000188542
PharmGKBiPA162384124

Polymorphism and mutation databases

BioMutaiDUSP28
DMDMi121943916

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003028401 – 176Dual specificity phosphatase 28Add BLAST176

Proteomic databases

EPDiQ4G0W2
PaxDbiQ4G0W2
PeptideAtlasiQ4G0W2
PRIDEiQ4G0W2
ProteomicsDBi62133

PTM databases

DEPODiQ4G0W2
iPTMnetiQ4G0W2
PhosphoSitePlusiQ4G0W2

Expressioni

Gene expression databases

BgeeiENSG00000188542 Expressed in 186 organ(s), highest expression level in deltoid
CleanExiHS_DUSP28
ExpressionAtlasiQ4G0W2 baseline and differential
GenevisibleiQ4G0W2 HS

Organism-specific databases

HPAiCAB069448
HPA047456

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

BioGridi130042, 31 interactors
STRINGi9606.ENSP00000344235

Structurei

Secondary structure

1176
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ4G0W2
SMRiQ4G0W2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini76 – 138Tyrosine-protein phosphataseAdd BLAST63

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1718 Eukaryota
COG2453 LUCA
GeneTreeiENSGT00760000118853
HOGENOMiHOG000233766
HOVERGENiHBG051422
InParanoidiQ4G0W2
KOiK14165
OMAiCINVSKQ
OrthoDBiEOG091G0RMR
PhylomeDBiQ4G0W2
TreeFamiTF105128

Family and domain databases

CDDicd00127 DSPc, 1 hit
Gene3Di3.90.190.10, 1 hit
InterProiView protein in InterPro
IPR000340 Dual-sp_phosphatase_cat-dom
IPR024950 DUSP
IPR029021 Prot-tyrosine_phosphatase-like
IPR000387 TYR_PHOSPHATASE_dom
IPR020422 TYR_PHOSPHATASE_DUAL_dom
PANTHERiPTHR10159 PTHR10159, 1 hit
PfamiView protein in Pfam
PF00782 DSPc, 1 hit
SMARTiView protein in SMART
SM00195 DSPc, 1 hit
SUPFAMiSSF52799 SSF52799, 1 hit
PROSITEiView protein in PROSITE
PS50056 TYR_PHOSPHATASE_2, 1 hit
PS50054 TYR_PHOSPHATASE_DUAL, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q4G0W2-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGPAEAGRRG AASPVPPPLV RVAPSLFLGS ARAAGAEEQL ARAGVTLCVN
60 70 80 90 100
VSRQQPGPRA PGVAELRVPV FDDPAEDLLA HLEPTCAAME AAVRAGGACL
110 120 130 140 150
VYCKNGRSRS AAVCTAYLMR HRGLSLAKAF QMVKSARPVA EPNPGFWSQL
160 170
QKYEEALQAQ SCLQGEPPAL GLGPEA
Length:176
Mass (Da):18,324
Last modified:August 30, 2005 - v1
Checksum:i3BB208F7373814B0
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
H7C234H7C234_HUMAN
Dual-specificity phosphatase 28
DUSP28
141Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC036198 mRNA Translation: AAH36198.1
CCDSiCCDS33418.1
RefSeqiNP_001028747.1, NM_001033575.1
UniGeneiHs.369297
Hs.672568

Genome annotation databases

EnsembliENST00000343217; ENSP00000344235; ENSG00000188542
ENST00000405954; ENSP00000385885; ENSG00000188542
GeneIDi285193
KEGGihsa:285193
UCSCiuc002vzg.3 human

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC036198 mRNA Translation: AAH36198.1
CCDSiCCDS33418.1
RefSeqiNP_001028747.1, NM_001033575.1
UniGeneiHs.369297
Hs.672568

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5Y15X-ray2.10A/B1-176[»]
5Y16X-ray2.40A/B1-176[»]
ProteinModelPortaliQ4G0W2
SMRiQ4G0W2
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi130042, 31 interactors
STRINGi9606.ENSP00000344235

PTM databases

DEPODiQ4G0W2
iPTMnetiQ4G0W2
PhosphoSitePlusiQ4G0W2

Polymorphism and mutation databases

BioMutaiDUSP28
DMDMi121943916

Proteomic databases

EPDiQ4G0W2
PaxDbiQ4G0W2
PeptideAtlasiQ4G0W2
PRIDEiQ4G0W2
ProteomicsDBi62133

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000343217; ENSP00000344235; ENSG00000188542
ENST00000405954; ENSP00000385885; ENSG00000188542
GeneIDi285193
KEGGihsa:285193
UCSCiuc002vzg.3 human

Organism-specific databases

CTDi285193
DisGeNETi285193
EuPathDBiHostDB:ENSG00000188542.9
GeneCardsiDUSP28
HGNCiHGNC:33237 DUSP28
HPAiCAB069448
HPA047456
neXtProtiNX_Q4G0W2
OpenTargetsiENSG00000188542
PharmGKBiPA162384124
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1718 Eukaryota
COG2453 LUCA
GeneTreeiENSGT00760000118853
HOGENOMiHOG000233766
HOVERGENiHBG051422
InParanoidiQ4G0W2
KOiK14165
OMAiCINVSKQ
OrthoDBiEOG091G0RMR
PhylomeDBiQ4G0W2
TreeFamiTF105128

Miscellaneous databases

GenomeRNAii285193
PROiPR:Q4G0W2

Gene expression databases

BgeeiENSG00000188542 Expressed in 186 organ(s), highest expression level in deltoid
CleanExiHS_DUSP28
ExpressionAtlasiQ4G0W2 baseline and differential
GenevisibleiQ4G0W2 HS

Family and domain databases

CDDicd00127 DSPc, 1 hit
Gene3Di3.90.190.10, 1 hit
InterProiView protein in InterPro
IPR000340 Dual-sp_phosphatase_cat-dom
IPR024950 DUSP
IPR029021 Prot-tyrosine_phosphatase-like
IPR000387 TYR_PHOSPHATASE_dom
IPR020422 TYR_PHOSPHATASE_DUAL_dom
PANTHERiPTHR10159 PTHR10159, 1 hit
PfamiView protein in Pfam
PF00782 DSPc, 1 hit
SMARTiView protein in SMART
SM00195 DSPc, 1 hit
SUPFAMiSSF52799 SSF52799, 1 hit
PROSITEiView protein in PROSITE
PS50056 TYR_PHOSPHATASE_2, 1 hit
PS50054 TYR_PHOSPHATASE_DUAL, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiDUS28_HUMAN
AccessioniPrimary (citable) accession number: Q4G0W2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: August 30, 2005
Last modified: November 7, 2018
This is version 103 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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