CYP27C1

Functionⁱ

Catalyzes the conversion of all-trans retinol (also called vitamin A1, the precursor of 11-cis retinal) to 3,4-didehydroretinol (also called vitamin A2, the precursor of 11-cis 3,4-didehydroretinal). Also acts on all-trans retinal and all-trans retinoic acid.1 Publication

Catalytic activityⁱ

All-trans-retinol + 2 reduced adrenodoxin + 2 H⁺ + O₂ = all-trans-3,4-didehydroretinol + 2 oxidized adrenodoxin + 2 H₂O.1 Publication

Cofactorⁱ

hemeBy similarity

Kineticsⁱ

K_M=
0.5 µM for all-trans retinol
1 Publication
Manual assertion based on experiment inⁱ
- Ref.5
  "Human cytochrome P450 27C1 catalyzes 3,4-desaturation of retinoids."
  Kramlinger V.M., Nagy L.D., Fujiwara R., Johnson K.M., Phan T.T., Xiao Y., Enright J.M., Toomey M.B., Corbo J.C., Guengerich F.P.
  FEBS Lett. 590:1304-1312(2016) [PubMed] [Europe PMC] [Abstract]
  Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.
K_M=
0.35 µM for all-trans retinal
1 Publication
Manual assertion based on experiment inⁱ
- Ref.5
  "Human cytochrome P450 27C1 catalyzes 3,4-desaturation of retinoids."
  Kramlinger V.M., Nagy L.D., Fujiwara R., Johnson K.M., Phan T.T., Xiao Y., Enright J.M., Toomey M.B., Corbo J.C., Guengerich F.P.
  FEBS Lett. 590:1304-1312(2016) [PubMed] [Europe PMC] [Abstract]
  Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.
K_M=
0.87 µM for all-trans retinoic acid
1 Publication
Manual assertion based on experiment inⁱ
- Ref.5
  "Human cytochrome P450 27C1 catalyzes 3,4-desaturation of retinoids."
  Kramlinger V.M., Nagy L.D., Fujiwara R., Johnson K.M., Phan T.T., Xiao Y., Enright J.M., Toomey M.B., Corbo J.C., Guengerich F.P.
  FEBS Lett. 590:1304-1312(2016) [PubMed] [Europe PMC] [Abstract]
  Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.

Sites

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Metal bindingⁱ	318	Iron (heme axial ligand)By similarity		1

GO - Molecular functionⁱ

11-cis-retinal 3,4-desaturase activity
Source: UniProtKB
Inferred from direct assayⁱ
- 27059013
11-cis retinal binding
Source: UniProtKB
Inferred from direct assayⁱ
- 27059013
all-trans retinal 3,4-desaturase activity
Source: UniProtKB
Inferred from direct assayⁱ
- 27059013
all-trans retinal binding
Source: UniProtKB
Inferred from direct assayⁱ
- 27059013
all-trans retinoic acid 3,4-desaturase activity
Source: UniProtKB
Inferred from direct assayⁱ
- 27059013
all-trans retinol 3,4-desaturase activity
Source: UniProtKB
Inferred from direct assayⁱ
- 27059013
all-trans-retinol binding
Source: UniProtKB
Inferred from direct assayⁱ
- 27059013
heme binding Source: InterPro
iron ion binding Source: InterPro
monooxygenase activity Source: UniProtKB-KW
retinoic acid binding
Source: UniProtKB
Inferred from direct assayⁱ
- 27059013

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

retinal metabolic process
Source: UniProtKB
Inferred from direct assayⁱ
- 27059013
retinoic acid metabolic process
Source: UniProtKB
Inferred from direct assayⁱ
- 27059013
retinol metabolic process
Source: UniProtKB
Inferred from direct assayⁱ
- 27059013

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Monooxygenase, Oxidoreductase
Ligand	Heme, Iron, Metal-binding

Chemistry databases

SwissLipidsⁱ	SLP:000001617

SwissLipidsⁱ

SLP:000001617

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Cytochrome P450 27C1 (EC:1.14.19.531 Publication) Alternative name(s): All-trans retinol 3,4-desaturase1 Publication
Gene namesⁱ	Name:CYP27C1
Organismⁱ	Homo sapiens (Human)
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Chromosome 2

Organism-specific databases

EuPathDBⁱ	HostDB:ENSG00000186684.12
Human Gene Nomenclature Database More...HGNCⁱ	HGNC:33480 CYP27C1
neXtProtⁱ	NX_Q4G0S4

Keywords - Cellular componentⁱ

Membrane

Pathology & Biotechⁱ

Organism-specific databases

Open Targets More...OpenTargetsⁱ	ENSG00000186684
PharmGKBⁱ	PA162383091

Polymorphism and mutation databases

BioMutaⁱ	CYP27C1
DMDMⁱ	296434415

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000293732	1 – 372	Cytochrome P450 27C1Add BLAST		372

Proteomic databases

EPDⁱ	Q4G0S4
PaxDbⁱ	Q4G0S4
PeptideAtlas More...PeptideAtlasⁱ	Q4G0S4
PRIDEⁱ	Q4G0S4
ProteomicsDBⁱ	62124

PTM databases

iPTMnetⁱ	Q4G0S4
PhosphoSitePlusⁱ	Q4G0S4

Expressionⁱ

Tissue specificityⁱ

Widely expressed, with highest levels in the liver, kidney and pancreas.1 Publication

Gene expression databases

Bgeeⁱ	ENSG00000186684 Expressed in 96 organ(s), highest expression level in tendon of biceps brachii
CleanExⁱ	HS_CYP27C1
ExpressionAtlasⁱ	Q4G0S4 baseline and differential
Genevisibleⁱ	Q4G0S4 HS

Organism-specific databases

Human Protein Atlas More...HPAⁱ	HPA062460 HPA068731

HPAⁱ

HPA062460
HPA068731

Interactionⁱ

Protein-protein interaction databases

BioGridⁱ	130927, 2 interactors
IntActⁱ	Q4G0S4, 1 interactor
STRINGⁱ	9606.ENSP00000334128

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q4G0S4
SMRⁱ	Q4G0S4
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

eggNOGⁱ	KOG0159 Eukaryota COG2124 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00900000140779
HOGENOMⁱ	HOG000253961
HOVERGENⁱ	HBG106909
InParanoidⁱ	Q4G0S4
KEGG Orthology (KO) More...KOⁱ	K17951
OMAⁱ	ALCHYAT
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0MI3
PhylomeDBⁱ	Q4G0S4
TreeFamⁱ	TF105094

Family and domain databases

Gene3Dⁱ	1.10.630.10, 1 hit
InterProⁱ	View protein in InterPro IPR001128 Cyt_P450 IPR017972 Cyt_P450_CS IPR002401 Cyt_P450_E_grp-I IPR036396 Cyt_P450_sf
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00067 p450, 1 hit
PRINTSⁱ	PR00463 EP450I PR00385 P450
SUPFAMⁱ	SSF48264 SSF48264, 1 hit
PROSITEⁱ	View protein in PROSITE PS00086 CYTOCHROME_P450, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Q4G0S4-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MRSVLRQRIL KPKDVAIYSG EVNQVIADLI KRIYLLRSQA EDGETVTNVN 
        60         70         80         90        100
DLFFKYSMEG VATILYESRL GCLENSIPQL TVEYIEALEL MFSMFKTSMY 
       110        120        130        140        150
AGAIPRWLRP FIPKPWREFC RSWDGLFKFS QIHVDNKLRD IQYQMDRGRR 
       160        170        180        190        200
VSGGLLTYLF LSQALTLQEI YANVTEMLLA GVDTTSFTLS WTVYLLARHP 
       210        220        230        240        250
EVQQTVYREI VKNLGERHVP TAADVPKVPL VRALLKETLR LFPVLPGNGR 
       260        270        280        290        300
VTQEDLVIGG YLIPKGTQLA LCHYATSYQD ENFPRAKEFR PERWLRKGDL 
       310        320        330        340        350
DRVDNFGSIP FGHGVRSCIG RRIAELEIHL VVIQLLQHFE IKTSSQTNAV 
       360        370 
HAKTHGLLTP GGPIHVRFVN RK

Length:372

Mass (Da):42,632

Last modified:May 18, 2010 - v2

Checksum:ⁱF6274FE61FCA3A8C

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	195	L → I in AAH39307 (PubMed:15815621).Curated		1

Natural variant

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Natural variantⁱVAR_033120	359	T → M. Corresponds to variant dbSNP:rs35075135Ensembl.		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AK131190 mRNA Translation: BAD18388.1 AC110926 Genomic DNA No translation available. BC039307 mRNA Translation: AAH39307.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS33285.1
NCBI Reference Sequences More...RefSeqⁱ	NP_001001665.3, NM_001001665.3
UniGeneⁱ	Hs.407639

Genome annotation databases

Ensemblⁱ	ENST00000335247; ENSP00000334128; ENSG00000186684 ENST00000409327; ENSP00000387198; ENSG00000186684
GeneIDⁱ	339761
KEGGⁱ	hsa:339761
UCSC genome browser More...UCSCⁱ	uc002tod.3 human

Keywords - Coding sequence diversityⁱ

Polymorphism

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q4G0S4	FLJ16008 protein, isoform CRA_a		HUMAN		372	UniRef100_Q4G0S4
UPI00003D4165		HUMAN		469
UPI00005B09F2		HUMAN		416

Protein	Similar proteins		Species	Score	Length	Source
Q4G0S4	FLJ16008 protein, isoform CRA_a		HUMAN		372	UniRef90_Q4G0S4
Uncharacterized protein		MACFA		542
Uncharacterized protein		AILME		476
Uncharacterized protein		SAIBB		372
CYP27C1 isoform 1		PANTR		372
+16

Protein	Similar proteins		Species	Score	Length	Source
Q4G0S4	Cytochrome P450 27C1		LITCT		537	UniRef50_Q4G0S4
Cytochrome P450 27C1		DANRE		540
FLJ16008 protein, isoform CRA_a		HUMAN		372
Uncharacterized protein		MACFA		542
Uncharacterized protein		AILME		476
+230

Cross-referencesⁱ

Web resourcesⁱ

Protein Spotlight

Seeing through the murk - Issue 192 of June 2017

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AK131190 mRNA Translation: BAD18388.1 AC110926 Genomic DNA No translation available. BC039307 mRNA Translation: AAH39307.1
CCDSⁱ	CCDS33285.1
RefSeqⁱ	NP_001001665.3, NM_001001665.3
UniGeneⁱ	Hs.407639

3D structure databases

ProteinModelPortalⁱ	Q4G0S4
SMRⁱ	Q4G0S4
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	130927, 2 interactors
IntActⁱ	Q4G0S4, 1 interactor
STRINGⁱ	9606.ENSP00000334128

Chemistry databases

SwissLipidsⁱ	SLP:000001617

SwissLipidsⁱ

SLP:000001617

PTM databases

iPTMnetⁱ	Q4G0S4
PhosphoSitePlusⁱ	Q4G0S4

Polymorphism and mutation databases

BioMutaⁱ	CYP27C1
DMDMⁱ	296434415

Proteomic databases

EPDⁱ	Q4G0S4
PaxDbⁱ	Q4G0S4
PeptideAtlasⁱ	Q4G0S4
PRIDEⁱ	Q4G0S4
ProteomicsDBⁱ	62124

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENST00000335247; ENSP00000334128; ENSG00000186684 ENST00000409327; ENSP00000387198; ENSG00000186684
GeneIDⁱ	339761
KEGGⁱ	hsa:339761
UCSCⁱ	uc002tod.3 human

Organism-specific databases

CTDⁱ	339761
EuPathDBⁱ	HostDB:ENSG00000186684.12
GeneCardsⁱ	CYP27C1
HGNCⁱ	HGNC:33480 CYP27C1
HPAⁱ	HPA062460 HPA068731
neXtProtⁱ	NX_Q4G0S4
OpenTargetsⁱ	ENSG00000186684
PharmGKBⁱ	PA162383091
GenAtlas: human gene database More...GenAtlasⁱ	Search...

Phylogenomic databases

eggNOGⁱ	KOG0159 Eukaryota COG2124 LUCA
GeneTreeⁱ	ENSGT00900000140779
HOGENOMⁱ	HOG000253961
HOVERGENⁱ	HBG106909
InParanoidⁱ	Q4G0S4
KOⁱ	K17951
OMAⁱ	ALCHYAT
OrthoDBⁱ	EOG091G0MI3
PhylomeDBⁱ	Q4G0S4
TreeFamⁱ	TF105094

Miscellaneous databases

ChiTaRSⁱ	CYP27C1 human
GeneWikiⁱ	CYP27C1
GenomeRNAiⁱ	339761
Protein Ontology More...PROⁱ	PR:Q4G0S4

Gene expression databases

Bgeeⁱ	ENSG00000186684 Expressed in 96 organ(s), highest expression level in tendon of biceps brachii
CleanExⁱ	HS_CYP27C1
ExpressionAtlasⁱ	Q4G0S4 baseline and differential
Genevisibleⁱ	Q4G0S4 HS

Family and domain databases

Gene3Dⁱ	1.10.630.10, 1 hit
InterProⁱ	View protein in InterPro IPR001128 Cyt_P450 IPR017972 Cyt_P450_CS IPR002401 Cyt_P450_E_grp-I IPR036396 Cyt_P450_sf
Pfamⁱ	View protein in Pfam PF00067 p450, 1 hit
PRINTSⁱ	PR00463 EP450I PR00385 P450
SUPFAMⁱ	SSF48264 SSF48264, 1 hit
PROSITEⁱ	View protein in PROSITE PS00086 CYTOCHROME_P450, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	C27C1_HUMAN
Accessionⁱ	Q4G0S4Primary (citable) accession number: Q4G0S4 Secondary accession number(s): Q6ZNI7
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
	Last sequence update:	May 18, 2010
	Last modified:	September 12, 2018
	This is version 105 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
Human chromosome 2
Human chromosome 2: entries, gene names and cross-references to MIM
Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations
Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations
Protein Spotlight
Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries

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UniProtKB - Q4G0S4 (C27C1_HUMAN)

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Cytochrome P450 27C1

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the ‘Function’ section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Chemistry databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Other locations

Other locations

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Organism-specific databases

Polymorphism and mutation databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Organism-specific databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Experimental Info

Natural variant

Sequence databases

Genome annotation databases

Keywords - Coding sequence diversityi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

<p>This subsection of the <a href="http://www.uniprot.org/manual/cross_references_section">Cross-references</a> section provides links to various web resources that are relevant for a specific protein.<p><a href='/help/web_resource' target='_top'>More...</a></p>Web resourcesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Chemistry databases

PTM databases

Polymorphism and mutation databases

Proteomic databases

Protocols and materials databases

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Organism-specific databases

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Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

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Functionⁱ

Kineticsⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Coding sequence diversityⁱ

Cross-referencesⁱ

Web resourcesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ