UniProtKB - Q4G0F8 (UBN1_MOUSE)
Protein
Ubinuclein-1
Gene
Ubn1
Organism
Mus musculus (Mouse)
Status
Functioni
Acts as a novel regulator of senescence. Involved in the formation of senescence-associated heterochromatin foci (SAHF), which represses expression of proliferation-promoting genes. Binds to proliferation-promoting genes. May be required for replication-independent chromatin assembly (By similarity).By similarity
GO - Molecular functioni
- DNA binding Source: UniProtKB-KW
- transcription factor binding Source: MGI
GO - Biological processi
- DNA replication-independent nucleosome assembly Source: MGI
- keratinocyte differentiation Source: MGI
Keywordsi
Molecular function | Chromatin regulator, DNA-binding |
Enzyme and pathway databases
Reactomei | R-MMU-2559584, Formation of Senescence-Associated Heterochromatin Foci (SAHF) |
Names & Taxonomyi
Protein namesi | Recommended name: Ubinuclein-1Alternative name(s): Ubiquitously expressed nuclear protein |
Gene namesi | Name:Ubn1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1891307, Ubn1 |
Subcellular locationi
Nucleus
- nucleoplasm By similarity
- PML body By similarity
Other locations
- tight junction 1 Publication
Note: Localized as a nuclear speckled-like pattern in proliferating primary fibroblasts. Colocalizes with HIRA, PML and SP100 in PML bodies of senescent cells. Colocalizes with CLDN1. Detected along the upper granular cell layer of epidermis. When overexpressed, accumulates in the nucleus in cells showing defective cytokinesis (By similarity). Colocalizes with TJP1.By similarity
Nucleus
- nuclear body Source: MGI
- nucleoplasm Source: MGI
- nucleus Source: MGI
- PML body Source: UniProtKB-SubCell
Other locations
- bicellular tight junction Source: UniProtKB
Keywords - Cellular componenti
Cell junction, Nucleus, Tight junctionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000370691 | 1 – 1135 | Ubinuclein-1Add BLAST | 1135 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 166 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 173 | PhosphoserineBy similarity | 1 | |
Modified residuei | 175 | PhosphoserineBy similarity | 1 | |
Modified residuei | 222 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 323 | PhosphoserineBy similarity | 1 | |
Modified residuei | 336 | PhosphoserineBy similarity | 1 | |
Modified residuei | 338 | PhosphoserineBy similarity | 1 | |
Modified residuei | 493 | PhosphoserineBy similarity | 1 | |
Modified residuei | 660 | PhosphoserineBy similarity | 1 | |
Modified residuei | 677 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1028 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
jPOSTi | Q4G0F8 |
PaxDbi | Q4G0F8 |
PeptideAtlasi | Q4G0F8 |
PRIDEi | Q4G0F8 |
PTM databases
iPTMneti | Q4G0F8 |
PhosphoSitePlusi | Q4G0F8 |
Expressioni
Tissue specificityi
Expressed in bile canaliculi in liver, in bronchiolar epithelium in lung and in tubular structures of gland ducts inside the olfactory epithelium and tongue epithelium.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000039473, Expressed in ear and 289 other tissues |
ExpressionAtlasi | Q4G0F8, baseline and differential |
Genevisiblei | Q4G0F8, MM |
Interactioni
Subunit structurei
Component of a complex that includes at least ASF1A, CABIN1, HIRA, histone H3.3 and UBN1.
Interacts with HIRA (via WD repeat domain); the interaction is direct.
Interacts with ASF1A, CEBPA, TJP1, TJP2 and TJP3 (By similarity).
By similarityGO - Molecular functioni
- transcription factor binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 228360, 2 interactors |
IntActi | Q4G0F8, 1 interactor |
MINTi | Q4G0F8 |
STRINGi | 10090.ENSMUSP00000061843 |
Miscellaneous databases
RNActi | Q4G0F8, protein |
Family & Domainsi
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 476 – 539 | Sequence analysisAdd BLAST | 64 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 181 – 261 | Lys-richAdd BLAST | 81 | |
Compositional biasi | 861 – 1032 | Ser-richAdd BLAST | 172 |
Sequence similaritiesi
Belongs to the ubinuclein family.Curated
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | KOG4786, Eukaryota |
GeneTreei | ENSGT00940000158857 |
HOGENOMi | CLU_007400_0_0_1 |
InParanoidi | Q4G0F8 |
OMAi | HQDPEPA |
OrthoDBi | 469344at2759 |
PhylomeDBi | Q4G0F8 |
TreeFami | TF326088 |
Family and domain databases
InterProi | View protein in InterPro IPR014840, HRD IPR026936, Ubinuclein-1 IPR026947, UBN_middle_dom |
PANTHERi | PTHR21669:SF12, PTHR21669:SF12, 1 hit |
Pfami | View protein in Pfam PF08729, HUN, 1 hit PF14075, UBN_AB, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
Isoform 1 (identifier: Q4G0F8-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSEPHRVQFT SVPGSLNPAF LKKSRKEEVG GTEQHQDCEP AAAAVRITLT
60 70 80 90 100
LFEPDHKRCP EFFYPELVKN IRGKVKGLHP GDKKKDVLDP FNDEEKERHK
110 120 130 140 150
VEALARKFEE KYGGKKRRKD RIQDLIDMGY GYDESDSFID NSEAYDELVP
160 170 180 190 200
ASLTTKYGGF YINSGTLQFR QASESEDDFI KEKKKKSPKK RKLKEGGEKI
210 220 230 240 250
KKKKKDDTYD KEKKSKKSKF SKAGFTALNA SKEKKKKKYS GSLSVREMLK
260 270 280 290 300
KFQKEKEAQK KREEEHKPVA VSSIEAQGLR ELEGTSDPLL SLFGSTSDND
310 320 330 340 350
LLQAATAMDS LTDLDLEQLL SESPEGSPFR DMDDGSDSLG VGLDQEFRQP
360 370 380 390 400
SSFPEGLPIP LEKRVKELAQ AARAAEGESK QKFFTQDING ILLDIEVQTR
410 420 430 440 450
ELTSQIRSGV FAYLASFLPC SKDALVKRAR KLHLYEQGGR LKEPLQKLKD
460 470 480 490 500
AIGRAMPEQV AKYQDECQAH TQAKVAKMLE EEKDKEQRER ICSDEEEDEE
510 520 530 540 550
KGGRRIMGPR KKFQWNDEIR ELLCQVVKIK LESRDLERNS KAQAWEDCVK
560 570 580 590 600
AFLDAEVKPL WPKGWMQART LFKESRRGHG HLTSLLAKKK VIAPSKIKMK
610 620 630 640 650
ESSVKLDKKV SVPSGQHGGP TTLLSEHQGG GLNTGANSRE HPSQATCGLT
660 670 680 690 700
DSVSVTLEDS LDEDLVRNPA SSVDAVSKEL ATLNSRAANS SEFTLPTPSK
710 720 730 740 750
APTEKVGGVL CTEEKRNFAK PSSSAPPPTN ALQSPLNFLA EQALALGQSS
760 770 780 790 800
QEKKPEGSGF KELSCQGPLS KGVPELHPSK AKHHNLPRTS HGPQAAAPVP
810 820 830 840 850
GPQVKVFHAG TQQQKSFTPP SPFVNKLQGP KATSPQCHRS LLQLVKTAAK
860 870 880 890 900
GQAFHATMPA SSGSSPASSS SAHKTTASNS TTISHPAKLH PTSSVGPSYK
910 920 930 940 950
NNPFAGSVSK HGASSSSPSP GGGAQVQSSV SGASLPGVQS PSAGQSASRA
960 970 980 990 1000
APSSAVKKTP VTQKLTLVAP PGGPNGDSGG GTQGVAKLLT SSLKPAAVSS
1010 1020 1030 1040 1050
VTSSTSLPKG TGGAVLLSNT SSLSLLSSSY KSNNPKLPGA MNSNSLGIIT
1060 1070 1080 1090 1100
QFPLHVLSFN ADSSAKAGVS KDAIVTGPAP GTFHHGLSHS LLAGLHSSPP
1110 1120 1130
HTAPLPHAAV STHVPQSLPD ASQLHGKGPV VPRKL
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A2R8VI01 | A0A2R8VI01_MOUSE | Ubinuclein-1 | Ubn1 | 231 | Annotation score: |
Sequence cautioni
The sequence EDK97277 differs from that shown. Reason: Erroneous gene model prediction.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 184 | K → N in BAB31587 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 681 | A → G in BAC39106 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 801 | G → A in BAC39106 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 969 | A → P in BAC39106 (PubMed:16141072).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_036972 | 1090 – 1120 | SLLAG…QSLPD → N in isoform 2. 1 PublicationAdd BLAST | 31 | |
Alternative sequenceiVSP_036973 | 1091 – 1135 | LLAGL…VPRKL → KFLSPASVPMLCAMLGPVCV WCNHGNIPHLWKLHSTRDSC FVPDWSAGCLGL in isoform 3. 1 PublicationAdd BLAST | 45 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK019173 mRNA Translation: BAB31587.1 AK020146 mRNA Translation: BAB32010.1 AK084046 mRNA Translation: BAC39106.1 AK137727 mRNA Translation: BAE23479.1 AK147571 mRNA Translation: BAE28001.1 CH466521 Genomic DNA Translation: EDK97277.1 Sequence problems. BC060723 mRNA Translation: AAH60723.1 BC098372 mRNA Translation: AAH98372.1 |
CCDSi | CCDS27931.1 [Q4G0F8-1] CCDS88873.1 [Q4G0F8-2] |
RefSeqi | NP_080942.1, NM_026666.3 [Q4G0F8-1] XP_006521879.1, XM_006521816.3 [Q4G0F8-1] XP_006521880.1, XM_006521817.3 [Q4G0F8-1] XP_006521882.1, XM_006521819.3 XP_017172375.1, XM_017316886.1 [Q4G0F8-1] |
Genome annotation databases
Ensembli | ENSMUST00000052449; ENSMUSP00000061843; ENSMUSG00000039473 [Q4G0F8-1] ENSMUST00000229126; ENSMUSP00000155263; ENSMUSG00000039473 [Q4G0F8-2] ENSMUST00000230703; ENSMUSP00000155223; ENSMUSG00000039473 [Q4G0F8-1] |
GeneIDi | 170644 |
KEGGi | mmu:170644 |
UCSCi | uc007ybq.1, mouse [Q4G0F8-1] uc007ybr.1, mouse [Q4G0F8-2] uc007ybs.1, mouse [Q4G0F8-3] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK019173 mRNA Translation: BAB31587.1 AK020146 mRNA Translation: BAB32010.1 AK084046 mRNA Translation: BAC39106.1 AK137727 mRNA Translation: BAE23479.1 AK147571 mRNA Translation: BAE28001.1 CH466521 Genomic DNA Translation: EDK97277.1 Sequence problems. BC060723 mRNA Translation: AAH60723.1 BC098372 mRNA Translation: AAH98372.1 |
CCDSi | CCDS27931.1 [Q4G0F8-1] CCDS88873.1 [Q4G0F8-2] |
RefSeqi | NP_080942.1, NM_026666.3 [Q4G0F8-1] XP_006521879.1, XM_006521816.3 [Q4G0F8-1] XP_006521880.1, XM_006521817.3 [Q4G0F8-1] XP_006521882.1, XM_006521819.3 XP_017172375.1, XM_017316886.1 [Q4G0F8-1] |
3D structure databases
SMRi | Q4G0F8 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 228360, 2 interactors |
IntActi | Q4G0F8, 1 interactor |
MINTi | Q4G0F8 |
STRINGi | 10090.ENSMUSP00000061843 |
PTM databases
iPTMneti | Q4G0F8 |
PhosphoSitePlusi | Q4G0F8 |
Proteomic databases
jPOSTi | Q4G0F8 |
PaxDbi | Q4G0F8 |
PeptideAtlasi | Q4G0F8 |
PRIDEi | Q4G0F8 |
Protocols and materials databases
Antibodypediai | 11234, 146 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000052449; ENSMUSP00000061843; ENSMUSG00000039473 [Q4G0F8-1] ENSMUST00000229126; ENSMUSP00000155263; ENSMUSG00000039473 [Q4G0F8-2] ENSMUST00000230703; ENSMUSP00000155223; ENSMUSG00000039473 [Q4G0F8-1] |
GeneIDi | 170644 |
KEGGi | mmu:170644 |
UCSCi | uc007ybq.1, mouse [Q4G0F8-1] uc007ybr.1, mouse [Q4G0F8-2] uc007ybs.1, mouse [Q4G0F8-3] |
Organism-specific databases
CTDi | 29855 |
MGIi | MGI:1891307, Ubn1 |
Phylogenomic databases
eggNOGi | KOG4786, Eukaryota |
GeneTreei | ENSGT00940000158857 |
HOGENOMi | CLU_007400_0_0_1 |
InParanoidi | Q4G0F8 |
OMAi | HQDPEPA |
OrthoDBi | 469344at2759 |
PhylomeDBi | Q4G0F8 |
TreeFami | TF326088 |
Enzyme and pathway databases
Reactomei | R-MMU-2559584, Formation of Senescence-Associated Heterochromatin Foci (SAHF) |
Miscellaneous databases
BioGRID-ORCSi | 170644, 2 hits in 19 CRISPR screens |
ChiTaRSi | Ubn1, mouse |
PROi | PR:Q4G0F8 |
RNActi | Q4G0F8, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000039473, Expressed in ear and 289 other tissues |
ExpressionAtlasi | Q4G0F8, baseline and differential |
Genevisiblei | Q4G0F8, MM |
Family and domain databases
InterProi | View protein in InterPro IPR014840, HRD IPR026936, Ubinuclein-1 IPR026947, UBN_middle_dom |
PANTHERi | PTHR21669:SF12, PTHR21669:SF12, 1 hit |
Pfami | View protein in Pfam PF08729, HUN, 1 hit PF14075, UBN_AB, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | UBN1_MOUSE | |
Accessioni | Q4G0F8Primary (citable) accession number: Q4G0F8 Secondary accession number(s): Q3UUZ4 Q9CS40 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 5, 2009 |
Last sequence update: | August 30, 2005 | |
Last modified: | December 2, 2020 | |
This is version 116 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families