UniProtKB - Q4FZC9 (SYNE3_MOUSE)
Nesprin-3
Syne3
Functioni
GO - Molecular functioni
- actin filament binding Source: UniProtKB
- cytoskeleton-nuclear membrane anchor activity Source: MGI
GO - Biological processi
- cytoskeleton organization Source: UniProtKB
- establishment of protein localization to membrane Source: UniProtKB
- nuclear migration Source: GO_Central
- nucleus localization Source: GO_Central
- regulation of cell shape Source: UniProtKB
Names & Taxonomyi
Protein namesi | Recommended name: Nesprin-3Alternative name(s): KASH domain-containing protein 3 Short name: KASH3 Nuclear envelope spectrin repeat protein 3 |
Gene namesi | Name:Syne3 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2442408, Syne3 |
Subcellular locationi
Endoplasmic reticulum
Nucleus
Endoplasmic reticulum
- rough endoplasmic reticulum Source: UniProtKB-SubCell
Nucleus
- meiotic nuclear membrane microtubule tethering complex Source: MGI
- nuclear envelope Source: UniProtKB
- nuclear membrane Source: MGI
- nuclear outer membrane Source: UniProtKB
Other locations
- cytoplasm Source: GO_Central
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 925 | CytoplasmicPROSITE-ProRule annotationAdd BLAST | 925 | |
Transmembranei | 926 – 946 | Helical; Anchor for type IV membrane proteinPROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 947 – 975 | Perinuclear spacePROSITE-ProRule annotationAdd BLAST | 29 |
Keywords - Cellular componenti
Endoplasmic reticulum, Membrane, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000281121 | 1 – 975 | Nesprin-3Add BLAST | 975 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 953 | Interchain (C-577 in SUN2); alternateBy similarity | ||
Disulfide bondi | 953 | Interchain (with C-759 in SUN1)By similarity |
Post-translational modificationi
Keywords - PTMi
Disulfide bondProteomic databases
EPDi | Q4FZC9 |
PaxDbi | Q4FZC9 |
PeptideAtlasi | Q4FZC9 |
PRIDEi | Q4FZC9 |
PTM databases
iPTMneti | Q4FZC9 |
PhosphoSitePlusi | Q4FZC9 |
SwissPalmi | Q4FZC9 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSMUSG00000054150, Expressed in retinal neural layer and 92 other tissues |
Genevisiblei | Q4FZC9, MM |
Interactioni
Subunit structurei
Core component of LINC complexes which are composed of inner nuclear membrane SUN domain-containing proteins coupled to outer nuclear membrane KASH domain-containing nesprins. SUN and KASH domain-containing proteins seem to bind each other promiscuously; however, differentially expression of LINC complex constituents can give rise to specific assemblies.
Interacts with SUN1 and SUN2; probably forming respective LINC complexes.
Interacts with PLEC (via actin-binding domain).
Interacts with DST.
Interacts with SYNE1.
Interacts (via KASH domain) with TOR1A (ATP-bound); the interaction is required for SYNE3 nuclear envelope localization.
4 PublicationsGO - Molecular functioni
- actin filament binding Source: UniProtKB
Protein-protein interaction databases
IntActi | Q4FZC9, 2 interactors |
STRINGi | 10090.ENSMUSP00000093090 |
Miscellaneous databases
RNActi | Q4FZC9, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 220 – 325 | Spectrin 1Add BLAST | 106 | |
Repeati | 647 – 740 | Spectrin 2Add BLAST | 94 | |
Domaini | 917 – 975 | KASHPROSITE-ProRule annotationAdd BLAST | 59 |
Domaini
Sequence similaritiesi
Keywords - Domaini
Repeat, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502QQSI, Eukaryota |
GeneTreei | ENSGT00440000039367 |
HOGENOMi | CLU_012764_0_0_1 |
InParanoidi | Q4FZC9 |
OMAi | RDIKAQW |
OrthoDBi | 87219at2759 |
PhylomeDBi | Q4FZC9 |
TreeFami | TF331132 |
Family and domain databases
InterProi | View protein in InterPro IPR012315, KASH IPR018159, Spectrin/alpha-actinin IPR002017, Spectrin_repeat IPR030267, SYNE3 |
PANTHERi | PTHR47535:SF2, PTHR47535:SF2, 1 hit |
Pfami | View protein in Pfam PF10541, KASH, 1 hit PF00435, Spectrin, 2 hits |
SMARTi | View protein in SMART SM01249, KASH, 1 hit SM00150, SPEC, 3 hits |
PROSITEi | View protein in PROSITE PS51049, KASH, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MTQQPQEDFE RSVEDAQAWM KVIQEQLQVN DNTKGPRAAL EARLRETEKI
60 70 80 90 100
CQLESEGMVK VELVLRAAEA LLATCQEGQK PEILARLRDI KSQWEETVTY
110 120 130 140 150
MTHCHSRIEW VWLHWSEYLL AQDEFYRWFQ KMVVALEPPV ELQLGLKEKQ
160 170 180 190 200
WQLSHAQVLL HNVDNQAVLL DRLLEEAGSL FSRIGDPSVD EDAQKRMKAE
210 220 230 240 250
YDAVKARAQR RVDLLAQVAQ DHEQYREDVN EFQLWLKAVV EKVHSCLGRN
260 270 280 290 300
CKLATELRLS TLQDIAKDFP RGEESLKRLE EQAVGVIQNT SPLGAEKISG
310 320 330 340 350
ELEEMRGVLE KLRVLWKEEE GRLRGLLQSR GDCEQQIQQL EAELGDFKKS
360 370 380 390 400
LQRLAQEGLE PTVKTATEDE LVAQWRLFSG TRAALASEEP RVDRLQTQLK
410 420 430 440 450
KLVTFPDLQS LSDSVVATIQ EYQSMKGKNT RLHNATRAEL WQRFQRPLND
460 470 480 490 500
LQLWKALAQR LLDITASLPD LASIHTFLPQ IEAALTESSR LKEQLAMLQL
510 520 530 540 550
KTDLLGSIFG QERAATLLEQ VTSSVRDRDL LHNSLLQRKS KLQSLLVQHK
560 570 580 590 600
DFGVAFDPLN RKLLDLQARI QAEKGLPRDL PGKQVQLLRL QGLQEEGLDL
610 620 630 640 650
GTQIEAVRPL AHGNSKHQQK VDQISCDQQA LQRSLEDLVD RCQQNVREHC
660 670 680 690 700
TFSHRLSELQ LWITMATQTL ESHQGDVRLW DAESQEAGLE TLLSEIPEKE
710 720 730 740 750
VQVSLLQALG QLVMKKSSPE GATMVQEELR KLMESWQALR LLEENMLSLM
760 770 780 790 800
RNQQLQRTEV DTGKKQVFTN NIPKAGFLIN PQDPIPRRQH GANPLEGHDL
810 820 830 840 850
PEDHPQLLRD FEQWLQAENS KLRRIITMRV ATAKDLRTRE VKLQELEARI
860 870 880 890 900
PEGQHLFENL LRLRPARDPS NELEDLRYRW MLYKSKLKDS GHLLTESSPG
910 920 930 940 950
ELTAFQKSRR QKRWSPCSLL QKACRVALPL QLLLLLFLLL LFLLPAGEEE
960 970
RSCALANNFA RSFALMLRYN GPPPT
Sequence cautioni
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_023979 | 1 – 87 | Missing in isoform 2. 1 PublicationAdd BLAST | 87 | |
Alternative sequenceiVSP_023980 | 88 – 105 | RDIKS…MTHCH → MTTRRGPERPWRQGFERQ in isoform 2. 1 PublicationAdd BLAST | 18 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK029216 mRNA Translation: BAC26351.1 Frameshift. AK030542 mRNA Translation: BAC27012.1 BC099694 mRNA Translation: AAH99694.1 |
CCDSi | CCDS36542.1 [Q4FZC9-1] CCDS36543.1 [Q4FZC9-2] |
RefSeqi | NP_001036164.1, NM_001042699.2 [Q4FZC9-1] NP_766088.2, NM_172500.3 [Q4FZC9-2] XP_006515715.2, XM_006515652.3 [Q4FZC9-1] XP_006515716.1, XM_006515653.2 [Q4FZC9-1] XP_006515717.1, XM_006515654.3 [Q4FZC9-1] |
Genome annotation databases
Ensembli | ENSMUST00000067005; ENSMUSP00000065771; ENSMUSG00000054150 [Q4FZC9-2] ENSMUST00000095439; ENSMUSP00000093090; ENSMUSG00000054150 [Q4FZC9-1] ENSMUST00000109927; ENSMUSP00000105553; ENSMUSG00000054150 [Q4FZC9-2] |
GeneIDi | 212073 |
KEGGi | mmu:212073 |
UCSCi | uc007oxr.2, mouse [Q4FZC9-1] uc007oxs.2, mouse [Q4FZC9-2] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK029216 mRNA Translation: BAC26351.1 Frameshift. AK030542 mRNA Translation: BAC27012.1 BC099694 mRNA Translation: AAH99694.1 |
CCDSi | CCDS36542.1 [Q4FZC9-1] CCDS36543.1 [Q4FZC9-2] |
RefSeqi | NP_001036164.1, NM_001042699.2 [Q4FZC9-1] NP_766088.2, NM_172500.3 [Q4FZC9-2] XP_006515715.2, XM_006515652.3 [Q4FZC9-1] XP_006515716.1, XM_006515653.2 [Q4FZC9-1] XP_006515717.1, XM_006515654.3 [Q4FZC9-1] |
3D structure databases
SMRi | Q4FZC9 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | Q4FZC9, 2 interactors |
STRINGi | 10090.ENSMUSP00000093090 |
PTM databases
iPTMneti | Q4FZC9 |
PhosphoSitePlusi | Q4FZC9 |
SwissPalmi | Q4FZC9 |
Proteomic databases
EPDi | Q4FZC9 |
PaxDbi | Q4FZC9 |
PeptideAtlasi | Q4FZC9 |
PRIDEi | Q4FZC9 |
Protocols and materials databases
Antibodypediai | 54248, 151 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000067005; ENSMUSP00000065771; ENSMUSG00000054150 [Q4FZC9-2] ENSMUST00000095439; ENSMUSP00000093090; ENSMUSG00000054150 [Q4FZC9-1] ENSMUST00000109927; ENSMUSP00000105553; ENSMUSG00000054150 [Q4FZC9-2] |
GeneIDi | 212073 |
KEGGi | mmu:212073 |
UCSCi | uc007oxr.2, mouse [Q4FZC9-1] uc007oxs.2, mouse [Q4FZC9-2] |
Organism-specific databases
CTDi | 161176 |
MGIi | MGI:2442408, Syne3 |
Phylogenomic databases
eggNOGi | ENOG502QQSI, Eukaryota |
GeneTreei | ENSGT00440000039367 |
HOGENOMi | CLU_012764_0_0_1 |
InParanoidi | Q4FZC9 |
OMAi | RDIKAQW |
OrthoDBi | 87219at2759 |
PhylomeDBi | Q4FZC9 |
TreeFami | TF331132 |
Miscellaneous databases
BioGRID-ORCSi | 212073, 4 hits in 17 CRISPR screens |
PROi | PR:Q4FZC9 |
RNActi | Q4FZC9, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000054150, Expressed in retinal neural layer and 92 other tissues |
Genevisiblei | Q4FZC9, MM |
Family and domain databases
InterProi | View protein in InterPro IPR012315, KASH IPR018159, Spectrin/alpha-actinin IPR002017, Spectrin_repeat IPR030267, SYNE3 |
PANTHERi | PTHR47535:SF2, PTHR47535:SF2, 1 hit |
Pfami | View protein in Pfam PF10541, KASH, 1 hit PF00435, Spectrin, 2 hits |
SMARTi | View protein in SMART SM01249, KASH, 1 hit SM00150, SPEC, 3 hits |
PROSITEi | View protein in PROSITE PS51049, KASH, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SYNE3_MOUSE | |
Accessioni | Q4FZC9Primary (citable) accession number: Q4FZC9 Secondary accession number(s): Q8BMM1, Q8C117 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 20, 2007 |
Last sequence update: | August 30, 2005 | |
Last modified: | February 10, 2021 | |
This is version 112 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families