UniProtKB - Q4DBX5 (ITPA1_TRYCC)
Protein
Inosine triphosphate pyrophosphatase 1
Gene
Tc00.1047053504103.6
Organism
Trypanosoma cruzi (strain CL Brener)
Status
Functioni
Pyrophosphatase that hydrolyzes non-canonical purine nucleotides such as inosine triphosphate (ITP), deoxyinosine triphosphate (dITP) or xanthosine 5'-triphosphate (XTP) to their respective monophosphate derivatives. The enzyme does not distinguish between the deoxy- and ribose forms. Probably excludes non-canonical purines from RNA and DNA precursor pools, thus preventing their incorporation into RNA and DNA and avoiding chromosomal lesions.UniRule annotation
Catalytic activityi
- a ribonucleoside 5'-triphosphate + H2O = a ribonucleoside 5'-phosphate + diphosphate + H+UniRule annotationEC:3.6.1.9UniRule annotation
- a 2'-deoxyribonucleoside 5'-triphosphate + H2O = a 2'-deoxyribonucleoside 5'-phosphate + diphosphate + H+UniRule annotationEC:3.6.1.9UniRule annotation
Cofactori
Mg2+UniRule annotation, Mn2+UniRule annotationNote: Binds 1 divalent metal cation per subunit; can use either Mg2+ or Mn2+.UniRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 48 | Magnesium or manganeseUniRule annotation | 1 | |
Binding sitei | 61 | SubstrateUniRule annotation | 1 | |
Metal bindingi | 77 | Magnesium or manganeseUniRule annotation | 1 | |
Binding sitei | 177 | SubstrateUniRule annotation | 1 |
GO - Molecular functioni
- metal ion binding Source: UniProtKB-KW
- NADH pyrophosphatase activity Source: UniProtKB-EC
- nucleoside-triphosphate diphosphatase activity Source: UniProtKB-UniRule
- nucleotide binding Source: UniProtKB-KW
GO - Biological processi
- deoxyribonucleoside triphosphate catabolic process Source: UniProtKB-UniRule
- nucleotide metabolic process Source: UniProtKB-KW
Keywordsi
Molecular function | Hydrolase |
Biological process | Nucleotide metabolism |
Ligand | Magnesium, Manganese, Metal-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Inosine triphosphate pyrophosphatase 1UniRule annotation (EC:3.6.1.9UniRule annotation)Short name: ITPase 1UniRule annotation Short name: Inosine triphosphatase 1UniRule annotation Alternative name(s): Non-canonical purine NTP pyrophosphatase 1UniRule annotation Non-standard purine NTP pyrophosphatase 1UniRule annotation Nucleoside-triphosphate diphosphatase 1UniRule annotation Nucleoside-triphosphate pyrophosphatase 1UniRule annotation Short name: NTPase 1UniRule annotation |
Gene namesi | ORF Names:Tc00.1047053504103.6 |
Organismi | Trypanosoma cruzi (strain CL Brener) |
Taxonomic identifieri | 353153 [NCBI] |
Taxonomic lineagei | Eukaryota › Euglenozoa › Kinetoplastida › Trypanosomatidae › Trypanosoma › Schizotrypanum › |
Proteomesi |
|
Subcellular locationi
- Cytoplasm UniRule annotation
GO - Cellular componenti
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000413123 | 1 – 196 | Inosine triphosphate pyrophosphatase 1Add BLAST | 196 |
Proteomic databases
PaxDbi | Q4DBX5 |
Interactioni
Subunit structurei
Homodimer.
UniRule annotationProtein-protein interaction databases
STRINGi | 5693.XP_811877.1 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 20 – 25 | Substrate bindingUniRule annotation | 6 | |
Regioni | 77 – 78 | Substrate bindingUniRule annotation | 2 | |
Regioni | 153 – 156 | Substrate bindingUniRule annotation | 4 |
Sequence similaritiesi
Belongs to the HAM1 NTPase family.UniRule annotation
Phylogenomic databases
eggNOGi | KOG3222 Eukaryota COG0127 LUCA |
KOi | K01519 |
OMAi | VGFGWDS |
OrthoDBi | 1298391at2759 |
Family and domain databases
CDDi | cd00515 HAM1, 1 hit |
Gene3Di | 3.90.950.10, 1 hit |
HAMAPi | MF_03148 HAM1_NTPase, 1 hit |
InterProi | View protein in InterPro IPR002637 Ham1p-like IPR027502 ITPase IPR029001 ITPase-like_fam |
PANTHERi | PTHR11067 PTHR11067, 1 hit |
Pfami | View protein in Pfam PF01725 Ham1p_like, 1 hit |
SUPFAMi | SSF52972 SSF52972, 1 hit |
TIGRFAMsi | TIGR00042 TIGR00042, 1 hit |
i Sequence
Sequence statusi: Complete.
Q4DBX5-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAEGSALLRG SSNHKVTLVT GNDGKRREVQ ACLEGHVLVE NVNLDLPEMQ
60 70 80 90 100
SDSVFEISRN KALMAYDITK SPVLVEDTAL CFDALGGLPG PYVKWFFERI
110 120 130 140 150
GPTGLIKLLE GFDTRRAYAT CVFTYCASPD VVLQFEGRCD GRIVEAPRGE
160 170 180 190
GGFGWDSVFE PDEGCGQTFA EMQDEEKNRI SPRAKALVAL KAHFCL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AAHK01000682 Genomic DNA Translation: EAN90026.1 |
RefSeqi | XP_811877.1, XM_806784.1 |
Genome annotation databases
EnsemblProtistsi | EAN90026; EAN90026; Tc00.1047053504103.6 |
GeneDBi | TcCLB.504103.6:pep |
GeneIDi | 3542909 |
KEGGi | tcr:504103.6 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AAHK01000682 Genomic DNA Translation: EAN90026.1 |
RefSeqi | XP_811877.1, XM_806784.1 |
3D structure databases
SMRi | Q4DBX5 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 5693.XP_811877.1 |
Proteomic databases
PaxDbi | Q4DBX5 |
Genome annotation databases
EnsemblProtistsi | EAN90026; EAN90026; Tc00.1047053504103.6 |
GeneDBi | TcCLB.504103.6:pep |
GeneIDi | 3542909 |
KEGGi | tcr:504103.6 |
Phylogenomic databases
eggNOGi | KOG3222 Eukaryota COG0127 LUCA |
KOi | K01519 |
OMAi | VGFGWDS |
OrthoDBi | 1298391at2759 |
Family and domain databases
CDDi | cd00515 HAM1, 1 hit |
Gene3Di | 3.90.950.10, 1 hit |
HAMAPi | MF_03148 HAM1_NTPase, 1 hit |
InterProi | View protein in InterPro IPR002637 Ham1p-like IPR027502 ITPase IPR029001 ITPase-like_fam |
PANTHERi | PTHR11067 PTHR11067, 1 hit |
Pfami | View protein in Pfam PF01725 Ham1p_like, 1 hit |
SUPFAMi | SSF52972 SSF52972, 1 hit |
TIGRFAMsi | TIGR00042 TIGR00042, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ITPA1_TRYCC | |
Accessioni | Q4DBX5Primary (citable) accession number: Q4DBX5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 19, 2011 |
Last sequence update: | September 13, 2005 | |
Last modified: | October 16, 2019 | |
This is version 65 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) |
Miscellaneousi
Keywords - Technical termi
Complete proteome, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families