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Protein

Alanine dehydrogenase

Gene

ald

Organism
Staphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM 20229)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the reversible reductive amination of pyruvate to L-alanine. May play a role in cell wall synthesis as L-alanine is an important constituent of the peptidoglycan layer (By similarity).By similarity

Catalytic activityi

L-alanine + H2O + NAD+ = pyruvate + NH3 + NADH.

Pathwayi: L-alanine degradation via dehydrogenase pathway

This protein is involved in step 1 of the subpathway that synthesizes NH(3) and pyruvate from L-alanine.
Proteins known to be involved in this subpathway in this organism are:
  1. Alanine dehydrogenase (ald)
This subpathway is part of the pathway L-alanine degradation via dehydrogenase pathway, which is itself part of Amino-acid degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes NH(3) and pyruvate from L-alanine, the pathway L-alanine degradation via dehydrogenase pathway and in Amino-acid degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei15SubstrateBy similarity1
Binding sitei74SubstrateBy similarity1
Active sitei95Proton donor/acceptorSequence analysis1
Binding sitei133NADBy similarity1
Binding sitei197NADBy similarity1
Binding sitei219NADBy similarity1
Active sitei269Proton donor/acceptorBy similarity1
Binding sitei279NADBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi177 – 178NADBy similarity2
Nucleotide bindingi238 – 239NADBy similarity2
Nucleotide bindingi266 – 269NADBy similarity4
Nucleotide bindingi298 – 301NADBy similarity4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
LigandNAD, Nucleotide-binding

Enzyme and pathway databases

BioCyciSSAP342451:G1G2H-1074-MONOMER.
UniPathwayiUPA00527; UER00585.

Names & Taxonomyi

Protein namesi
Recommended name:
Alanine dehydrogenase (EC:1.4.1.1)
Gene namesi
Name:ald
Ordered Locus Names:SSP1057
OrganismiStaphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM 20229)
Taxonomic identifieri342451 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000006371 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002873251 – 371Alanine dehydrogenaseAdd BLAST371

Interactioni

Subunit structurei

Homohexamer. Trimer of dimer (By similarity).By similarity

Protein-protein interaction databases

STRINGi342451.SSP1057.

Structurei

3D structure databases

ProteinModelPortaliQ49YD9.
SMRiQ49YD9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AlaDH/PNT family.Curated

Phylogenomic databases

eggNOGiENOG4105DCF. Bacteria.
COG0686. LUCA.
HOGENOMiHOG000022120.
KOiK00259.
OMAiCFETSKA.
OrthoDBiPOG091H02R2.

Family and domain databases

CDDicd05305. L-AlaDH. 1 hit.
InterProiView protein in InterPro
IPR008141. Ala_DH.
IPR008143. Ala_DH/PNT_CS2.
IPR007886. AlaDH/PNT_N.
IPR007698. AlaDH/PNT_NAD(H)-bd.
IPR036291. NAD(P)-bd_dom_sf.
PANTHERiPTHR42795. PTHR42795. 1 hit.
PfamiView protein in Pfam
PF01262. AlaDh_PNT_C. 1 hit.
PF05222. AlaDh_PNT_N. 1 hit.
PIRSFiPIRSF000183. Alanine_dh. 1 hit.
SMARTiView protein in SMART
SM01002. AlaDh_PNT_C. 1 hit.
SM01003. AlaDh_PNT_N. 1 hit.
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR00518. alaDH. 1 hit.
PROSITEiView protein in PROSITE
PS00837. ALADH_PNT_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q49YD9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIIGIPKEIK NNENRVSLSP SGVHALVEQG HTVIVEKSAG LGSYFEDVDY
60 70 80 90 100
TEAGASIVNE QAEVWNVDMV MKVKEPLEEE FQYFKEGLIL FTYLHLANEE
110 120 130 140 150
KLTRALLENK VVGIAYETVQ LPDRTLPLLT PMSEVAGRMS AQIGAEFLQK
160 170 180 190 200
YKGGMGILLG GVPGVSKGRV SIIGGGQAGT NAAKIALGLG ADVTILDVNP
210 220 230 240 250
KRLQELEDLF DGRVHTIMSN PLNIEQCVKD SDLVIGAVLI PGAKAPNLVT
260 270 280 290 300
EDMVKEMRDG AVIVDIAIDQ GGIFETTDRI STHDDPTYKK HGVVHYAVAN
310 320 330 340 350
MPGAVPRTST IALNNATLPY AQQLASKGYL KALQDNHALS LGLNTINGEL
360 370
TNKGVAEALN LSYTDIESAL K
Length:371
Mass (Da):39,903
Last modified:September 13, 2005 - v1
Checksum:i9BC859AAC3B4AF64
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008934 Genomic DNA. Translation: BAE18202.1.
RefSeqiWP_011302900.1. NZ_MTGA01000033.1.

Genome annotation databases

EnsemblBacteriaiBAE18202; BAE18202; SSP1057.
GeneIDi3615425.
KEGGissp:SSP1057.
PATRICifig|342451.11.peg.1056.

Similar proteinsi

Entry informationi

Entry nameiDHA_STAS1
AccessioniPrimary (citable) accession number: Q49YD9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: September 13, 2005
Last modified: February 28, 2018
This is version 92 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome