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UniProtKB - Q49HM9 (GRK7A_DANRE)
Protein
Rhodopsin kinase grk7a
Gene
grk7a
Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Functioni
Retina-specific kinase involved in the shutoff of the photoresponse and adaptation to changing light conditions via cone opsin phosphorylation, including rhodopsin (RHO).
1 PublicationMiscellaneous
Although the protein is present in a diversity of vertebrates ranging from bony fish to mammals, the mouse and rat orthologous proteins do not exist.
Catalytic activityi
Kineticsi
- KM=4.4 µM for rhodopsin1 Publication
- Vmax=773 nmol/min/mg enzyme1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 215 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 311 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 192 – 200 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- G protein-coupled receptor kinase activity Source: InterPro
- photoreceptor activity Source: ZFIN
- protein kinase activity Source: GO_Central
- rhodopsin kinase activity Source: ZFIN
GO - Biological processi
- cone photoresponse recovery Source: ZFIN
- phototransduction, visible light Source: ZFIN
- protein phosphorylation Source: GO_Central
- regulation of signal transduction Source: GO_Central
- visual perception Source: ZFIN
Keywordsi
Molecular function | Kinase, Serine/threonine-protein kinase, Transferase |
Biological process | Sensory transduction, Vision |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 2.7.11.14, 928 |
Reactomei | R-DRE-2514859, Inactivation, recovery and regulation of the phototransduction cascade |
SABIO-RKi | Q49HM9 |
Names & Taxonomyi
Protein namesi | Recommended name: Rhodopsin kinase grk7a (EC:2.7.11.141 Publication)Alternative name(s): G protein-coupled receptor kinase 7-1 G-protein-coupled receptor kinase 7A |
Gene namesi | Name:grk7a Synonyms:grk7-1 ORF Names:dkeyp-13a3.1 |
Organismi | Danio rerio (Zebrafish) (Brachydanio rerio) |
Taxonomic identifieri | 7955 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Danionidae › Danioninae › Danio |
Proteomesi |
|
Organism-specific databases
ZFINi | ZDB-GENE-050824-1, grk7a |
Subcellular locationi
Other locations
- Membrane By similarity; Lipid-anchor By similarity
Keywords - Cellular componenti
MembranePathology & Biotechi
Disruption phenotypei
Impaired cone response recovery and delayed dark adaptation.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000412812 | 1 – 546 | Rhodopsin kinase grk7aAdd BLAST | 546 | |
PropeptideiPRO_0000412813 | 547 – 549 | Removed in mature formBy similarity | 3 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 33 | PhosphoserineBy similarity | 1 | |
Modified residuei | 546 | Cysteine methyl esterSequence analysis | 1 | |
Lipidationi | 546 | S-geranylgeranyl cysteineSequence analysis | 1 |
Post-translational modificationi
Phosphorylation at Ser-33 is regulated by light and activated by cAMP.By similarity
Keywords - PTMi
Lipoprotein, Methylation, Phosphoprotein, PrenylationProteomic databases
PaxDbi | Q49HM9 |
PRIDEi | Q49HM9 |
Expressioni
Gene expression databases
Bgeei | ENSDARG00000020602, Expressed in retina and 8 other tissues |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 53 – 171 | RGSPROSITE-ProRule annotationAdd BLAST | 119 | |
Domaini | 186 – 449 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 264 | |
Domaini | 450 – 515 | AGC-kinase C-terminalPROSITE-ProRule annotationAdd BLAST | 66 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 522 – 549 | DisorderedSequence analysisAdd BLAST | 28 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 524 – 539 | Basic and acidic residuesSequence analysisAdd BLAST | 16 |
Sequence similaritiesi
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.Curated
Phylogenomic databases
eggNOGi | KOG0986, Eukaryota |
GeneTreei | ENSGT00940000160511 |
HOGENOMi | CLU_000288_63_41_1 |
InParanoidi | Q49HM9 |
OMAi | MSRVVFY |
OrthoDBi | 1104340at2759 |
PhylomeDBi | Q49HM9 |
TreeFami | TF313940 |
Family and domain databases
Gene3Di | 1.10.167.10, 1 hit |
InterProi | View protein in InterPro IPR000961, AGC-kinase_C IPR000239, GPCR_kinase IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR016137, RGS IPR036305, RGS_sf IPR044926, RGS_subdomain_2 IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit PF00615, RGS, 1 hit |
PRINTSi | PR00717, GPCRKINASE |
SMARTi | View protein in SMART SM00315, RGS, 1 hit SM00133, S_TK_X, 1 hit SM00220, S_TKc, 1 hit |
SUPFAMi | SSF48097, SSF48097, 1 hit SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS51285, AGC_KINASE_CTER, 1 hit PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit PS50132, RGS, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q49HM9-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MCDMGGLDNL VANTAYLKAQ GGDDKEMKKR RRSLSLPKPE QCASLRTSLD
60 70 80 90 100
KDFESLCEKQ PIGKKLFRQY LSQGGPECTT AAEFLDDLNE WELSESAARD
110 120 130 140 150
KARTNIINKF CKEGSKSSLT FLTGDVATKC KAVSDKDFEE VMGQVKTATK
160 170 180 190 200
EFLKGKPFTE YQASPFFDKF LQWKEYEKQP ISEKYFYEFR TLGKGGFGEV
210 220 230 240 250
CAVQVKNTGQ MYACKKLCKK RLKKKHGEKM ALLEKKILER VNSLFIVSLA
260 270 280 290 300
YAYDTKTHLC LVMSLMNGGD LKYHIYNIGE KGIEMDRIIY YTAQIATGIL
310 320 330 340 350
HLHDMDIVYR DMKPENVLLD SQGQCRLSDL GLAVEIAVGK TISQKAGTGA
360 370 380 390 400
YMAPEILNET PYRTSVDWWA LGCSIYEMVA GYTPFKGPDA KKEKVEKEEV
410 420 430 440 450
QRRILNEEPK FEHKNFDAAT IDIIKQFLKK KIDERLGCKN DDPRKHEWFK
460 470 480 490 500
SINFARLEAG LIDPPWVPKP NVVYAKDTGD IAEFSEIKGI EFDAKDDKFF
510 520 530 540
KEFSTGAVSI AWQQEMIDTG LFDELSDPNR KESSGGSDDD KKSGTCTLL
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 80 | T → I in AAX69081 (PubMed:16039565).Curated | 1 | |
Sequence conflicti | 385 | F → I in AAX69081 (PubMed:16039565).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY900004 mRNA Translation: AAX69081.1 AB212995 mRNA Translation: BAE92858.1 CR377211 Genomic DNA Translation: CAQ13370.1 BC163587 mRNA Translation: AAI63587.1 |
RefSeqi | NP_001027011.2, NM_001031841.3 |
Genome annotation databases
Ensembli | ENSDART00000100287; ENSDARP00000091060; ENSDARG00000020602 |
GeneIDi | 566120 |
KEGGi | dre:566120 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY900004 mRNA Translation: AAX69081.1 AB212995 mRNA Translation: BAE92858.1 CR377211 Genomic DNA Translation: CAQ13370.1 BC163587 mRNA Translation: AAI63587.1 |
RefSeqi | NP_001027011.2, NM_001031841.3 |
3D structure databases
SMRi | Q49HM9 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 7955.ENSDARP00000091060 |
Proteomic databases
PaxDbi | Q49HM9 |
PRIDEi | Q49HM9 |
Genome annotation databases
Ensembli | ENSDART00000100287; ENSDARP00000091060; ENSDARG00000020602 |
GeneIDi | 566120 |
KEGGi | dre:566120 |
Organism-specific databases
CTDi | 566120 |
ZFINi | ZDB-GENE-050824-1, grk7a |
Phylogenomic databases
eggNOGi | KOG0986, Eukaryota |
GeneTreei | ENSGT00940000160511 |
HOGENOMi | CLU_000288_63_41_1 |
InParanoidi | Q49HM9 |
OMAi | MSRVVFY |
OrthoDBi | 1104340at2759 |
PhylomeDBi | Q49HM9 |
TreeFami | TF313940 |
Enzyme and pathway databases
BRENDAi | 2.7.11.14, 928 |
Reactomei | R-DRE-2514859, Inactivation, recovery and regulation of the phototransduction cascade |
SABIO-RKi | Q49HM9 |
Miscellaneous databases
PROi | PR:Q49HM9 |
Gene expression databases
Bgeei | ENSDARG00000020602, Expressed in retina and 8 other tissues |
Family and domain databases
Gene3Di | 1.10.167.10, 1 hit |
InterProi | View protein in InterPro IPR000961, AGC-kinase_C IPR000239, GPCR_kinase IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR016137, RGS IPR036305, RGS_sf IPR044926, RGS_subdomain_2 IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit PF00615, RGS, 1 hit |
PRINTSi | PR00717, GPCRKINASE |
SMARTi | View protein in SMART SM00315, RGS, 1 hit SM00133, S_TK_X, 1 hit SM00220, S_TKc, 1 hit |
SUPFAMi | SSF48097, SSF48097, 1 hit SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS51285, AGC_KINASE_CTER, 1 hit PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit PS50132, RGS, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | GRK7A_DANRE | |
Accessioni | Q49HM9Primary (citable) accession number: Q49HM9 Secondary accession number(s): Q1XHL8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 21, 2011 |
Last sequence update: | September 21, 2011 | |
Last modified: | February 23, 2022 | |
This is version 110 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families