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Protein

Omega-amidase NIT2

Gene

Nit2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Has a omega-amidase activity. The role of omega-amidase is to remove potentially toxic intermediates by converting alpha-ketoglutaramate and alpha-ketosuccinamate to biologically useful alpha-ketoglutarate and oxaloacetate, respectively.2 Publications

Catalytic activityi

A monoamide of a dicarboxylate + H2O = a dicarboxylate + NH3.1 Publication

Kineticsi

In solution, alpha-ketoglutaramate is in equilibrium with a cyclic form (2-hydroxy-5-oxoproline), and at pH 8.0 or above, the rate of ring opening is no longer limiting for the omega-amidase reaction.
  1. KM=0.0054 mM for alpha-ketoglutaramate (open-chain form)1 Publication
  1. Vmax=20.6 µmol/min/mg enzyme with alpha-ketoglutaramate as substrate1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei43Proton acceptorPROSITE-ProRule annotation1
Active sitei112Proton donorPROSITE-ProRule annotation1
Active sitei153NucleophilePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase

Enzyme and pathway databases

ReactomeiR-RNO-6798695 Neutrophil degranulation

Names & Taxonomyi

Protein namesi
Recommended name:
Omega-amidase NIT2 (EC:3.5.1.31 Publication)
Alternative name(s):
Nitrilase homolog 2
Gene namesi
Name:Nit2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 11

Organism-specific databases

RGDi1310494 Nit2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003202561 – 276Omega-amidase NIT2Add BLAST276

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei26PhosphoserineBy similarity1
Modified residuei68N6-acetyllysine; alternateBy similarity1
Modified residuei68N6-succinyllysine; alternateBy similarity1
Modified residuei123N6-succinyllysineBy similarity1
Modified residuei130N6-succinyllysineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ497B0
PRIDEiQ497B0

PTM databases

iPTMnetiQ497B0
PhosphoSitePlusiQ497B0

Expressioni

Gene expression databases

BgeeiENSRNOG00000027797 Expressed in 10 organ(s), highest expression level in adult mammalian kidney
GenevisibleiQ497B0 RN

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

BioGridi252479, 1 interactor
STRINGi10116.ENSRNOP00000034144

Structurei

3D structure databases

ProteinModelPortaliQ497B0
SMRiQ497B0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 248CN hydrolasePROSITE-ProRule annotationAdd BLAST245

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0806 Eukaryota
COG0388 LUCA
GeneTreeiENSGT00550000074838
HOGENOMiHOG000222700
HOVERGENiHBG105126
InParanoidiQ497B0
KOiK13566
OMAiTGKDHWQ
OrthoDBiEOG091G0IKZ
PhylomeDBiQ497B0
TreeFamiTF300747

Family and domain databases

Gene3Di3.60.110.10, 1 hit
InterProiView protein in InterPro
IPR003010 C-N_Hydrolase
IPR036526 C-N_Hydrolase_sf
PfamiView protein in Pfam
PF00795 CN_hydrolase, 1 hit
SUPFAMiSSF56317 SSF56317, 1 hit
PROSITEiView protein in PROSITE
PS50263 CN_HYDROLASE, 1 hit

Sequencei

Sequence statusi: Complete.

Q497B0-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSTFRLALIQ LQVSSIKSDN ITRACSLVRE AAKQGANIVS LPECFNSPYG
60 70 80 90 100
TNYFPEYAEK IPGESTKKLS EVAKENSIYL IGGSIPEEDD GKLYNTCAVF
110 120 130 140 150
GPDGNLLVKH RKIHLFDIDV PGKITFQESK TLSPGDSFST FDTPYCRVGL
160 170 180 190 200
GICYDMRFAE LAQIYARRGC QLLVYPGAFN MTTGPAHWEL LQRARAVDNQ
210 220 230 240 250
VYVATASPAR DEKASYVAWG HSTVVDPWGQ VLTKAGTEET ILYSDIDLKK
260 270
LSEIRQQIPI LKQKRADLYS VESKKP
Length:276
Mass (Da):30,701
Last modified:September 13, 2005 - v1
Checksum:i38C64D674B171EF3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC100637 mRNA Translation: AAI00638.1
RefSeqiNP_001029298.1, NM_001034126.1
UniGeneiRn.42859

Genome annotation databases

EnsembliENSRNOT00000029420; ENSRNOP00000034144; ENSRNOG00000027797
GeneIDi288174
KEGGirno:288174
UCSCiRGD:1310494 rat

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC100637 mRNA Translation: AAI00638.1
RefSeqiNP_001029298.1, NM_001034126.1
UniGeneiRn.42859

3D structure databases

ProteinModelPortaliQ497B0
SMRiQ497B0
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi252479, 1 interactor
STRINGi10116.ENSRNOP00000034144

PTM databases

iPTMnetiQ497B0
PhosphoSitePlusiQ497B0

Proteomic databases

PaxDbiQ497B0
PRIDEiQ497B0

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000029420; ENSRNOP00000034144; ENSRNOG00000027797
GeneIDi288174
KEGGirno:288174
UCSCiRGD:1310494 rat

Organism-specific databases

CTDi56954
RGDi1310494 Nit2

Phylogenomic databases

eggNOGiKOG0806 Eukaryota
COG0388 LUCA
GeneTreeiENSGT00550000074838
HOGENOMiHOG000222700
HOVERGENiHBG105126
InParanoidiQ497B0
KOiK13566
OMAiTGKDHWQ
OrthoDBiEOG091G0IKZ
PhylomeDBiQ497B0
TreeFamiTF300747

Enzyme and pathway databases

ReactomeiR-RNO-6798695 Neutrophil degranulation

Miscellaneous databases

PROiPR:Q497B0

Gene expression databases

BgeeiENSRNOG00000027797 Expressed in 10 organ(s), highest expression level in adult mammalian kidney
GenevisibleiQ497B0 RN

Family and domain databases

Gene3Di3.60.110.10, 1 hit
InterProiView protein in InterPro
IPR003010 C-N_Hydrolase
IPR036526 C-N_Hydrolase_sf
PfamiView protein in Pfam
PF00795 CN_hydrolase, 1 hit
SUPFAMiSSF56317 SSF56317, 1 hit
PROSITEiView protein in PROSITE
PS50263 CN_HYDROLASE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiNIT2_RAT
AccessioniPrimary (citable) accession number: Q497B0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: September 13, 2005
Last modified: October 10, 2018
This is version 82 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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